Allelopathic effects ofPolygonum aviculare L.

We previously reported thatPolygonum aviculare has a strong allelopathic action againstCynodon dactylon (L.) Pers. and other test species. Moreover, we found that several phenolic compounds appeared to be important allelochemics in this activity. We have now isolated other potential inhibitors fromP. aviculare residues and soil underPolygonum stands, and none of these occurred in soil underC. dactylon stands. GC-MS analysis revealed that these additional inhibitors are long-chain fatty acids with 14–22 carbons. Nine were identified inP. aviculare residues and seven in soil underP. aviculare. Sodium salts of all the identified fatty acids inhibited seedling growth ofC. dactylon and at least some test strains of the nitrogen-fixing bacteria,Azotobacter andRhizobium.     

Allelopathic effects ofPolygonum aviculare L. I. Vegetational patterning

Polygonum aviculare was observed to spread rapidly into heavy stands ofCynodon dactylon (L.) Pers. resulting in death of the latter. This indicated a strong interference againstCynodon dactylon. Measurements of selected soil minerals and physical factors indicated that competition was probably not the chief cause of that interference. Soil collected under deadPolygonum was very inhibitory to all test species exceptSporobolus pyramidatus (Lam.) Hitchc., suggesting the presence of inhibitory compounds. Tops and roots ofPolygonum, root exudates, and leachate of the tops inhibited seed germination and seedling growth of most test species. Therefore, allelopathy apeared to be the dominant component of the interference, with competition probably accentuating its effects.Polygonum aviculare was inhibitory toGossypium barbadense L. andSorghum bicolor (L.) Moench, indicating that allelopathy is an important component of the interference byPolygonum against crop yields.     

Allelopathic effects ofPolygonum aviculare L. II. Isolation,characterization, and biological activities of phytotoxins

In earlier work, we found thatPolygonum aviculare had pronounced allelopathic effects against several test species. Four inhibitors were isolated from livingPolygonum plants, three of which were glucosides. Four different inhibitors were isolated fromPolygonum residues and soil underPolygonum stands, and none of these occurred in soil fromCynodon dactylon (L.) Pers. stands. Three of these were glycosides containing both fructose and cellobiose as the sugars. Color reactions of all the inhibitors indicated that they are phenolic in nature. All the inhibitors reduced seed germination and/or seedling growth ofChenopodium album L. Moreover some of them inhibited growth of different strains ofRhizobium andAzotobacter.     

Allelopathic effect of alfalfa (Medicago sativa) on bladygrass (Imperata cylindrica)

Greenhouse and laboratory experiments were conducted at the Agricultural and Water Resources Research Center Station, Baghdad, in 1985 and 1986 to investigate the possible allelopathic potential of alfalfa (Medicago saliva L.) and its decomposed residues on bladygrass (Imperata cylin-drica L. Beauv.), a noxious weed in Iraq, and to isolate, characterize, and quantify possible allelopathic agents in alfalfa residues and root exudates. Results indicated that decomposed alfalfa roots and their associated soil produced a 51–56% reduction in bladygrass seed germination. Root and shoot length of bladygrass seedlings were reduced by an average of 88%. Decayed and undecayed mixtures of alfalfa roots and soil at 0.0151 (w/w) inhibited bladygrass seedlings reproduced from rhizomes by 30 and 42%. It was found that root exudates of alfalfa seedlings caused significant reduction in shoot and root dry weights of bladygrass seedlings when alfalfa and bladygrass were grown together in nutrient culture. Caffeic, chlorogenic, isochloro-genic,p-coumaric,p-OH-benzoic, and ferulic acids were detected in alfalfa root exudates and residues. The highest amount (126 fig phenolic acids/g soil) of these compounds was found in alfalfa root residues after six months of decomposition in soil.     

Allelopathic potential ofnuphar lutea (L.) Sibth. & Sm. (Nymphaeaceae)

Aqueous extracts ofNuphar lutea (L.) Sibth. & Sm. leaves (blades plus petioles) and roots plus rhizomes were tested for allelopathic activity using lettuce seedling andLemna minor L. assay systems. The 12.5, 25, 125, and 250 parts per thousand (ppt) treatments of both extracts killed the lettuce seedlings. At 2.5 ppt of extract, radicle growth of lettuce was 29% of the control for leaves and 31% of the control for roots plus rhizomes.Lemna minor frond number was reduced to 34% of the control by the 25 ppt leaf extract and to 43% of the control by the 25 ppt roots plus rhizomes extract.L. minor was killed by concentrations of 125 ppt and above of both plant part extracts. As expected, the frond number and total chlorophyll content measured by theL. minor assay were highly correlated. Osmotic potentials below 143 mOsmol/kg had no influence onL. minor growth. Neither the osmotic potential nor the pH of the undiluted extracts ofN. lutea were in the range known to influence the growth of either lettuce seedlings orL. minor. Nuphar lutea extracts were many times more inhibitory than 16 other hydrophytes we previously examined.     

Effect of gamma irradiation on allelopathic potential ofSorghum bicolor against weeds and nitrification

The effect of low doses of gamma irradiation on the allelopathic potential ofSorghum bicolor against weeds and nitrification was investigated. The results revealed that all test doses (500, 1000 and 1500 rad) significantly increased the allelopathic activity of root exudates, aqueous extracts, and decaying residues aganst seed germination and seedling growth ofAmaranthus retroflexus. The results also indicated that all test doses stimulated the allelopathic potential of aqueous extracts and decaying residues against nitrification activity. The possible application of this approach in biological control is briefly discussed.     

Allelopathic potentials of alfalfa (Medicago sativa) saponins: Their relation to antifungal and hemolytic activities

A wheat seedling bioassay was used to indicate the relationship between the chemical structure of alfalfa saponins and their allelopathic activity. None of the tested saponins significantly influenced wheat germination. Seedling growth bioassays indicated significant differences among the activities of individual glycosides. The most active were medicagenic acid, its glycosides substituted at the C-3 position with glucose, and hederagenin monoglycoside. Medicagenic acid glycosides, substituted at the C-3 position with glucuronic acid, and zahnic acid tridesmoside were less active. No significant correlation was found among the allelopathic, hemolytic, and antifungal activities of the individual glycosides.     

Allelopathic effect of parthenium (Parthenium hysterophorus L.) extract and residue on some agronomic crops and weeds

Allelopathic effects of entire shoot extract, plant part extracts, and shoot residue of parthenium (Parthenium hysterophorus L.) on corn (Zea mays L.), ryegrass (Lolium multiflorum Lam.), wheat (Triticum aestivum L.), velvetleaf (Abutilon theophrasti Medik.), and soybean [Glycine max (L) Merr.] growth were examined. Parthenium shoot contained water-soluble materials that were toxic to root growth of velvetleaf and wheat. At 4% (w/ v) concentration, root growth of velvetleaf and wheat were reduced by 60 and 75%, respectively. The order of increasing sensitivity to parthenium was ryegrass, corn, wheat, and velvetleaf. There was a strong correlation between extract concentration and increased toxicity to test species. The toxicity of plant part extracts was also concentration dependent. At 1 and 2% (w/v), the inflorescence and leaves caused more root inhibition than stem extract. Parthenium shoot incorporated in soil at 1% (w/w) caused significantly more root inhibition of wheat than soybean, corn, and ryegrass. At 4% (w/w), root growth of all the test species was inhibited compared to the control. Toxicity of parthenium residue to wheat diminished with increasing periods of decomposition. Residue decomposed for four weeks was less toxic than the undecomposed residue.Florida Agricultural Experiment Stations Journal Series No. 7506.     

Evaluation of some weed extracts against field dodder on alfalfa (Medicago sativa)

Experiments were conducted to evaluate the effectiveness of some weed extracts against field dodderCuscuta campestris Yunck. on alfalfa (Medicago stiva L.) and to identify and quantify the phytotoxic agents of these extracts. All concentrations of aqueous extract of every weed showed significant effectiveness on dodder when compared to the untreated plant under lath house and field conditions. Control percentages of the (0.5 g) of Bermuda grass (Cynodon dactylon) and wall goosefoot (Chenopodium murale) ranged between 83 and 96, and the same concentration caused injury to alfalfa foliage up to 43% when applied in the field. Phytotoxic agents were identified as phenolic compounds such as chlorogenic, isochlorogenic,p-coumaric acids, and scopoletin. Their quantities varied with species; Bermuda grass had the highest content (32.2 g/g dry weight) followed by Johnson grass (Sorghum halepense), well goosefoot, and tumble pigweed (Amaranthus albus). These results might aid in screening for effective alternate approaches for controlling dodder on alfalfa planted for seeds.     

Biological suppression of nitrification by selected cultivars ofHelianthus annum L.

The allelopathic potential of different cultivars ofHelianthus annuus against nitrification was studied using the soil incubation method. The results indicated that aqueous extracts and residues of roots and shoots of all test cultivars significantly reduced the nitrification rate in soil. However, cultivars Local and Citosol were found to be more inhibitory to nitrification than others.     

Allelopathic potential of Macaranga tanarius (L.) muell.-arg

Macaranga tanarius is widely distributed in the abandoned lowlands of Taiwan where substantial amounts of leaves accumulate on the ground. A unique pattern of weed exclusion underneath trees is often found and thought to result from allelopathic interactions. Density-dependent phytotoxicity analysis of Lactuca sativa L. (lettuce) growing in soil mixed with the powder of M. tanarius leaves showed a significant deviation from the expected yield–density relationship. Lettuce growth was most suppressed in the low seed density experiment suggesting that the phytotoxins produced during leaf decomposition inhibit the growth of lettuce seedlings. Bidens pilosa and Leucaena leucocephala, growing in soil mixed with the leaf powder of M. tanarius were also suppressed. Aqueous leaf extracts were bioassayed against lettuce and B. pilosa, and exhibited a significant suppression in radicle growth. Compounds identified from leaves included nymphaeol-A (1), nymphaeol-B (2), nymphaeol-C (3), quercetin (4), abscisic acid (ABA) (5), blumenol A (6), blumenol B (7), roseoside II (8), tanariflavanone A (9), and tanariflavanone B (10). ABA was the major growth inhibitor. At concentrations of 20 ppm, ABA suppressed lettuce germination, while at 120 ppm it inhibited the growth of Miscanthus floridulus, Chloris barbata, and Bidens pilosa. At 600 ppm, quercetin, blumenol A, and blumenol B, caused 20–25% inhibition of radicle and shoot growth of M. floridulus. The amount of ABA in M. tanarius leaves was approximately 3–5 g g^–1 dry weight, significantly higher than previously reported. We conclude that the pattern of weed exclusion underneath stands of M. tanarius and its invasion into its adjacent grassland vegetation results from allelopathic interactions.     

Nitrogen requirements of corn (Zea mays L.) as affected by monocropping and intercropping with Alfalfa (Medicago sativa)

Intercropping perennials with corn has the potential to improve utilization of the growing season over monocropping corn in regions where a substantial portion of the growing season is too cool for corn growth. The biomass potential and fertilizer N requirements of monocropped corn (Zea mays) grown using conventional tillage were compared with those of corn intercropped with alfalfa (Medicago sativa) in 1987 and 1988. The intercropped alfalfa was harvested once prior to planting the corn each spring. Rotation effects on and N fertilizer requirements for monocropped corn following these treatments and also following monocropped alfalfa, were evaluated in 1989 and 1990. During the two years of intercropping for which data is presented, the critical intercropped corn biomass (13.05 Mg ha^–1) estimated using a quadratic-plus plateau model, was close to the monocropped corn biomass (13.01 Mg ha^–1), but an estimated 83 kg ha^–1 more N was required for intercropped corn to reach the critical biomass. Total biomass (intercropped corn and alfalfa) was 25% greater than that of the monocropped corn, and the total N uptake was 55% greater than that by monocropped corn over the two- year period. After rotation to monocropped corn using conventional tillage in 1989, corn biomass averaged over N rates following intercropping or monocropped corn was lower (P=0.01) than following monocropped alfalfa. Critical corn biomass estimated was highest following alfalfa and lowest following monocropped corn, and more N fertilizer was required to attain the critical biomass under continuous monocropped corn in 1989. Corn yields and N uptake values in 1990 were not significantly different among the cropping systems. The N fertilizer replacement values due to intercropping decreased from above 90 kg N ha^–1 in the first year of rotation to less than 40 kg N ha^–1 in the second year of rotation. Considering the higher potential for total biomass production and rotation benefit, intercropping is a viable alternative to conventional corn monoculture for forage production.     

Allelopathic compounds in leaves ofGliricidia sepium (Jacq.) kunth ex walp. and its effect onSorghum vulgare L.

Allelochemicals fromGliricidia sepium were extracted, identified, and quantified using HPLC. Fifteen toxic compounds, namely gallic acid, protocatechuic acid,p-hydroxybenzoic acid, gentisic acid,-resorcyclic acid, vanillic acid, syringic acid,p-coumaric acid,m-coumaric acid,o-coumaric acid, ferulic acid, sinapinic acid (trans andcis forms), coumarin, and myricetin were identified and quantified. These compounds from the plant extracts were tested on the seeds of the crop plant,Sorghum vulgare. Rate of germination of the seeds and root elongation were found to be inhibited by the various compounds of the extract. Different quantities ofGliricidia leaf mulch, viz., 400, 800, and 1200 g/m² applied to theSorghum grown fields, were found to effectively control weeds. Mulching improved the total yield ofSorghum. Leaf manuring and mulching showed better crop yield when applied up to 800 g ofGliricidia leaf/m². Crop yield was better in mulch-applied fields when compared to the manure-applied ones.     

Seed-germination inhibition by volatile alcohols and other compounds associated withAmaranthus palmeri residues

Effects of 3-pentanone and eight low molecular weight aliphatic alcohols and aldehydes identifed in the mixtures of volatiles released byAmaranthus palmeri S. Wats. (AMAPA) residues were determined on germination of onion, carrot, AMAPA, and tomato seeds. Three-day exposures to these volatiles significantly inhibited germination of these assay seeds, and the inhibition was dependent upon exposure time and concentration. Based on the degree of inhibition observed in both time- and concentration-dependent assays, the following activity series was obtained: 2-heptanol > 3-methyl-1-butanol, 1-hexanol > hexanal, 1-pentanol, 3-pentanone, acetaldehyde > ethanol, 2-methyl-1-propanol. The activities of this group of compounds with that of 2-heptanone appear to be additive and related to test compound volatility and hydrophilicity. 2-Heptanol and 2-heptanone also significantly inhibited the germination of other species, including shepherdspurse, soybean, lettuce, alfalfa, common purslane, oats, and lovegrass.Name of companies or commercial products are given solely for the purpose of providing specific information; their mention does not imply recommendation or endorsement by the U.S. Department of Agriculture over others not mentioned.     

Electroantennogram responses ofCampoletis sonorensis (hymenoptera: Ichneumonidae) to chemicals in cotton (Gossypium hirsutum L.)

Combined gas chromatography-electroantennogram (GC-EAG) recording ofCampoletis sonorensis (Cameron) responses to cotton plant volatile chemicals was performed.C. sonorensis antennal olfactory receptors respond differentially to green leaf, mono-, and sesquiterpene chemicals that have been identified previously in cotton. EAG depolarizations to green leaf chemicals were greater than to terpenes.     

Inhibition of seedling growth of crop species by recirculating root exudates ofBidens pilosa L.

The allelopathic effects of root exudates ofBidens pilosa L. on seedling growth ofLactuca sativa L.,Phaseolus vulgaris L.,Zea mays L., andSorghum bicolor (L.) Moench were studied using a root exudate recirculating system that allows continuous exposure of crop plants to allelopathic chemicals. This system maintains an undisturbed rhizosphere and eliminates competition and physical contact between the donor and acceptor plants. Comparison of responses to hydrophobic and hydrophilic root exudates is made possible by removal of hydrophobic compounds using XAD-4. Treatments consisted ofB. pilosa, B. pilosa with an Amberlite XAD-4 resin column attached to the donor pot to remove hydrophobic allelochemicals, and a donor pot without weeds.B. pilosa significantly inhibited seedling growth of all crop species tested. The crop species varied in response to the root exudates, withL. sativa being most sensitive. Larger and olderB. pilosa plants caused greater inhibition of seedling growth ofL. sativa andP. vulgaris than did smaller (younger)B. pilosa plants.B. pilosa with XAD-4 caused significantly less inhibition to all crop species, exceptZ. mays, thanB. pilosa without XAD-4, indicating that the hydrophobic exudates played an important role in the allelopathic growth inhibition. Variability in species response toB. pilosa with and without XAD-4 was probably due to differences in sensitivity to hydophobic and hydrophilic allelochemicals.Supported by a grant under USDA Agreement No. 83-CRSR-2-2293. Journal Series No. 2887 of the Hawaii Institute of Tropical Agriculture and Human Resources.     

CHANGES OVER TIME IN THE ALLELOCHEMICAL CONTENT OF TEN CULTIVARS OF RYE (<Emphasis Type="Italic">Secale cereale</Emphasis> L.)

Published studies focused on characterizing the allelopathy-based weed suppression by rye cover crop mulch have provided varying and inconsistent estimates of weed suppression. Studies were initiated to examine several factors that could influence the weed suppressiveness of rye: kill date, cultivar, and soil fertility. Ten cultivars of rye were planted with four rates of nitrogen fertilization, and tissue from each of these treatment combinations was harvested three times during the growing season. Concentrations of a known rye allelochemical DIBOA (2,4-dihydroxy-1,4-(2H)benzoxazine-3-one) were quantified from the harvested rye tissue using high performance liquid chromatography (HPLC). Phytotoxicity observed from aqueous extracts of the harvested rye tissue correlated with the levels of DIBOA recovered in harvested tissue. The amount of DIBOA in rye tissue varied depending on harvest date and rye cultivar, but was generally lower with all cultivars when rye was harvested later in the season. However, the late maturing variety Wheeler retained greater concentrations of DIBOA in comparison to other rye cultivars when harvested later in the season. The decline in DIBOA concentrations as rye matures, and the fact that many rye cultivars mature at different rates may help explain why estimates of weed suppression from allelopathic agents in rye have varied so widely in the literature.     

Effects of Water Deficit Stress, Shade, Weed Competition, and Kaolin Particle Film on Selected Foliar Free Amino Acid Accumulations in Cotton, Gossypium hirsutum (L.)

Leaves of cotton plants, Gossypium hirsutum L., stressed by water deficit, reduced daylight, and weed competition, or treated with a kaolin wettable powder formulation were analyzed for levels of 17 free amino acids (FAAs) using reversed-phase high-performance liquid chromatography. Water deficit stress resulted in heightened free proline levels (49.9-fold, P < 0.001) that were correlated with diffusive resistance (seconds per centimeter). Five other FAAs increased, and the amounts of total free essential (for insect growth and development) amino acids and total FAAs also increased (P 0.05). Cotton grown in 50% shade accumulated significantly more free arginine than control plants. In a small-plot weed competition assay, four FAAs increased and three FAAs decreased in association with weed competition, but because free proline levels were not altered and free arginine levels increased, other stresses aside from water deficit, possibly including shading by tall weeds, appear to have caused the changes. In a small-plot kaolin particle film assay, five FAAs were lower in cotton foliage sprayed weekly with kaolin. Because free proline was unaffected and free arginine was lower, it is possible that kaolin's reflectivity heightened light reception. The responses of free proline and arginine to the treatments used in these assays demonstrate that types and degrees of some stresses to cotton can be characterized by accumulations of certain FAAs. The study also demonstrates how some FAA levels can indicate degrees of cotton stress resulting from weed competition and from kaolin particle film application. Porometry and leaf water potential measurements assisted in corroborating some findings of the study.     

Metabolism and exudation of canavanine during development of alfalfa (Medicago sativa L. cv. verko)

The structural analog of amino acidl-arginine,l-canavanine (2-amino-4-guanidinooxybutyric acid), is found in 26 cultivars of alfalfa. Its concentration ranges from 6 to 16 mg/g of dry seeds. Canavanine represented more than 70% of the total soluble nitrogen in seeds. Practically all of the canavanine was stored in the cotyledons. Comparison is made of the canavanine content in the cultivars Verko and Europa harvested in different years. During sprouting, 29% of the guanidinooxy compound was translocated into the hypocotyl and radicle in 24 hr. In this early stage of seedling development, the level of the nonprotein amino acid, canavanine, increased threefold whereas the protein amino acid, arginine, as well as asparagine increased 11- and 35-fold, respectively. Two-day-old seedlings are capable of synthesizing canavanine derived from canaline up to 25%. Contrary to this finding in seedlings grown in the time range of 24 days, the guanidino compounds canavanine and arginine were metabolized rapidly, whereas asparagine increased. Furthermore, the toxic canavanine got into the environment of swelled seeds or into the rhizosphere of young seedlings and increased in the milieu to concentrations at 3–57M. In a biotest, this inhibited the growth of a tomato cell suspension culture as well as the growth of cabbage radicle.     

Growth response,yield and yield components of upland cotton (Gossypium hirsutum L) as affected by rates and time of nitrogen application in the Nigerian savannah

Effects of rate and time of nitrogen fertilization on growth, yield and yield components of upland cotton (Gossypium hirsutum L) were studied in two years (1975–76). Four rates of nitrogen application (0, 26, 52 and 78 kg ha^–1) timed at 3 or 8 weeks after sowing were compared. Seed cotton yield components increased significantly with increased N application at least up to 52 kg N ha^–1, with yield increases between 49% and 73%. Seed cotton yield was influenced by treatments mainly through boll number. Both crop growth rate and fruiting were enhanced by nitrogen fertilization. Applying N at 8 weeks (flowering) favoured yield only slightly over that at 3 weeks (thinning), but improved crop growth and fruiting by about 64% and 24%, respectively. There were significant N rate × time interactions in favour of fertilization at flowering. Applying 52 kg N ha^–1 at 8 weeks seems best for cotton in the Nigerian savannah.