保健食品中总黄酮的提取及含量测定研究

建立了保健食品中总黄酮含量测定的分光光度法,研究了黄酮的最佳工艺条件.结果表明以芦丁为标准品所建立的保健食品中总黄酮含量的测定方法简便可行,重现性好.采用单因素分析法和正交实验L9(3 4),对保健食品中总黄酮的提取工艺进行了研究,得出片剂和粉荆中总黄酮最佳提取条件为:70%乙醇、温度80℃、料液比1:25(g/mL),提取时间2 h,提取次数为两次.     

金鸡菊总黄酮的提取及含量测定

建立了金鸡菊总黄酮含量测定的分光光度法，研究了黄酮提取的最佳工艺条件。结果表明：金鸡菊花中总黄酮含量为25．99％，以芦丁为标准品所建立的金鸡菊总黄酮含量的测定方法简便可行，重现性好。黄酮提取的最佳工艺条件为水浴温度859，提取时间1．5h，提取3次，乙醇浓度65％。     

新疆酸梅中黄酮类化合物的提取工艺研究

NaNO3、Al(NO3)3、NaOH与黄酮类化合物作用后生成红色络合物,以芦丁为标准品,使用分光光度计测定酸梅中总黄酮的含量并研究了黄酮提取的工艺条件.结果表明:酸梅中总黄酮含量为0.887%.黄酮提取的最佳工艺条件为乙醇浓度为65%、提取时间为2h、提取次数1次、水浴温度85℃.利用此法简便可行,重现性好.     

鸭儿芹总黄酮提取及含量测定研究

本文建立了鸭儿芹总黄酮含量测定的分光光度法,研究了黄酮提取的最佳工艺条件。结果表明:鸭儿芹总黄酮含量为4.47%,以芦丁为标准品所建立的鸭儿芹总黄酮含量的测定方法简便可行,重现性好。采用单因素和L9(34)正试实验设计对鸭儿芹总黄酮提取工艺进行了研究,得出鸭儿芹总黄酮最佳提取条件为:乙醇体积分数70%、温度70℃、料液比(g/ml)1:20、提取时间1.5h、提取3次。     

八角茴香总黄酮提取工艺研究

以八角为原料,研究乙醇回流法提取八角总黄酮。采用单因素和正交实验确定八角黄酮的最佳提取条件。结果:八角黄酮最佳提取条件为乙醇体积分数60%、提取时间2h、料液比1∶40、提取温度80℃。结论:在最佳工艺条件下,八角总黄酮得率为13.34%。     

蒲公英总黄酮的提取工艺及定性分析

探讨了蒲公英总黄酮的提取工艺.在单因素实验的基础上,通过正交实验,确定总黄酮提取的最佳工艺条件为:乙醇浓度75%,温度70℃,液料比28 :1,时间60min,在此条件下,总黄酮含量达到3.869%(以芦丁计).通过颜色反应和紫外光谱法对提取物进行了定性分析,结果表明,提取物中存在黄酮类化合物,其类型主要为黄酮醇类,还可能含有黄酮或二氢黄酮类.     

香椿叶总黄酮的微波提取及其清除DPPH自由基能力的研究

目的:研究微波辅助提取香椿叶中总黄酮的工艺条件和提取物清除DPPH自由基的能力.方法:在单因素试验的基础上,采用L16(45)正交试验法对香椿叶的微波提取工艺进行优选.以黄酮提取量为主参考指标,提取物以黄酮计的清除DPPH的IC50值为次参考指标,考察乙醇体积分数、固液比、微波提取时间、提取温度、提取次数对香椿叶总黄酮提取量的影响.结果:最佳工艺条件为:20 g原料,以料液比1:12加入70%的乙醇溶液,在80℃下每次微波提取20 min,共提取4次.在最佳参数组合下,每克香椿叶可提取总黄酮25.7705 mg,提取物以黄酮计的清除DPPH的IC50值为37.1073.结论:该工艺是微波法提取香椿叶总黄酮的最佳工艺.     

超声波提取豆荚总黄酮优化工艺与抗氧化性研究

以大豆豆荚为原料,以超声波法提取黄酮化合物.结果表明,豆荚黄酮的最佳提取工艺条件为:乙醇浓度70%,料液比1:25(w/v)、萃取时间20 min、超声波功率270 W、浸提次数为3次.在此最佳工艺条件下进行试验,测得豆荚总黄酮得率为8.49%.豆荚总黄酮具有较强的体外抗氧化活性.     

微波法提取新疆罗布麻叶总黄酮及其抗氧化能力的研究

采用L9(34)正交试验对新疆罗布麻叶的微波提取工艺进行优选,以黄酮提取量为参考指标,确定最佳提取工艺条件为:1:12(g/mL)的80%乙醇水溶液,70%微波提取10min,提取4次,罗布麻叶提取总黄酮含量为56mg/g.研究了提取物的抗氧化能力,新疆罗布麻叶总黄酮对DPPH自由基有强的清除活性,IC50值为6.7ug/mL,抗氧化效果优于芦丁次于VC.     

西番莲叶总黄酮的提取工艺研究

以总黄酮得率为考察指标,采用乙醇浸提法,在单因素实验的基础上,通过正交试验对西番莲叶中黄酮的提取工艺进行优化.结果表明其最佳提取工艺为:温度80℃,乙醇浓度60%,料液比1:50,提取时间为60min.此工艺条件下总黄酮得率可达2.81%.     

蛋白质水解度测定方法综述

对目前国内外常用的蛋白质水解度测定方法进行了综述，其中pH—state方法是通过滴定水解过程中释放的质子测定DH；OPA、TNBS及国内常用的水合茚三酮和甲醛等测定方法是利用游离氨基的反应测定DH。     

SEROLOGIC IDENTIFICATION OF SPECIES OF ORIGIN OF SAUSAGE MEATS

SUMMARY: A partially purified immunoglobulin G (lgG) solution prepared from the serum of species to be tested was heated to the specifications for sausages. The resulting supernatant fluid was decanted and the precipitate washed with saline and used to immunize rabbits. The supernatant fluid was used to sensitize tanned sheep red blood cells. The immune serum was rendered monospecific by absorptions with heterologous, heated lgG precipitates. A sample of monospecific immune serum was absorbed with a washed homogenate of sausage. Aliquots of the monospecific immune serum, both untreated and sausage absorbed, were tested with cells sensitized with the homologous heated lgG supematant fluid. A significant reduction of titer by sausage absorption indicated that the sausages contained the meat homologous to the immune serum.     

BASIS OF STABILITY OF AMINE SALTS OF LINOLEIC ACID

SUMMARY— The mechanism and generality of the known stabilization against autoxidation conferred on linoleic acid by certain basic amino acids, such as lysine and arginine, was investigated. Basic amino acids were the only class of compounds found to confer the effect. However, the smallest basic amino acid, 2,3-diaminopropionic acid, was not effective, nor was αβω-diaminc acid, 3,6-diaminohexanoic acid, although a simple isomer of lysine. The stabilization was observed only in the solid phase. Inclusion of sodium chloride in the solid matrix was deleterious to the effect. A large number of physical and chemical observations were made and correlated but it has not been possible to draw detailed conclusions about the mechanism of stabilization, nor can a detailed structure of the stabilized complex be suggested. The cause of the phenomenon appears to be closely associated with the physical arrangement of the ions in the crystal lattice.     

聚多元羧酸盐的合成及应用

研究了聚多元羧酸盐的合成方法及反应机理,将其应用于洗涤剂和PVC制品中分别代替三聚磷酸钠和邻苯二甲酸二丁酯,证明有良好效果。     

百年风尚

一场流光溢彩、赏心悦目的展览,一段百年风尚演进的传奇旅程,一次东西方文化艺术的完美对话。2013年9月13日,“博萃臻艺一中西方珍宝艺术展”在辽宁省博物馆举行了隆重的开幕仪式,法兰西共和国驻华大使白林女士、辽宁省文物局局长丁辉先生、辽宁省博物馆馆长马宝杰先生、卡地亚全球总裁兼首席执行官邓阁仕先生、卡地亚区域行政总裁（北亚洲）陆慧全先生、卡地亚中国区首席执行官陆意斯先生、辽宁省文物店总经理张春鹰先生,以及众多文化界与文博界的贵宾齐聚一堂,共同见证了这场文化艺术盛事。     

STABILITY OF AQUEOUS EMULSIONS OF THE ESSENTIAL OIL OF HOPS

Hop oil emulsions prepared from different varieties of hops have been found to exhibit enhanced physical stability on the addition of blends of the emulsifiers Span 20/Tween 80 or Span 60/Tween 60. Examination of the particle size and volume distributions of an emulsion by use of a Coulter Counter was found to be an excellent method of monitoring its stability. An indication as to the relative efficiency of emulsifiers can be obtained from Coulter Counter measurements on hop oil emulsions after storage for 4 days. The use of an ultracentrifuge provies a rapid means of testing emulsion stability and hence the effectiveness of emulsion stabilizers.     

EFFECTS OF TYPES OF FAT AND OF RATES AND TEMPERATURES OF COMMINUTION ON DISPERSION OF LIPIDS IN FRANKFURTERS

ABSTRACT— The effect of time, temperature and rpm of comminution of emulsions was determined on the dispersion of approximately 25% of beef fat, pork fat or cottonseed oil in frankfurters. The numbers of lipid particles 5 μ or less in diameter increased in frankfurters containing either beef or pork fat as comminution was continued to higher temperatures, with pork fat dispersed more thoroughly. Fat tended to separate from frankfurters containing beef fat in particles 200 μ or more in diameter. In contrast, no specific degree of dispersion of particles 5 μ or less in diameter consistently indicated emulsion stability, or its lack. Increased rpm during comminution produced an increased dispersion of beef or pork fat. Under the same conditions pork fat was dispersed more finely than beef fat. Dispersion of cottonseed oil produced finely dispersed particles beyond the resolution of light microscopy, as was confirmed by electron microscopy which showed a substantial number of particles to be less than 1 μ in diameter.     

DEVELOPMENT OF CHAINS OF CELLS OF SACCHAROMYCES CEREVISIAE DURING FERMENTATION

The lengths of chains of cells of Saccharomyces cerevisiae were studied during fermentation. Pitching yeast generally contained about half of the total number of cells as two-celled chains. The chain lengths varied during the subsequent fermentation and the variations were characteristic of the strain. Electronic counting assessments of chain length were unreliable.     

ANALYTICAL CHARACTERIZATION OF THE PRODUCTS OF NONENZYMATIC GLYCOSYLATION OF LYSOZYME

The quantitative analysis of the reaction products of the water activity dependent nonenzymatic glycosylation of lysozyme was not straightforward. Difficulties arose in the determination of the number of bound glucose molecules because glycosylation leads to glucose mediated protein aggregation, and the likely presence of a mixture of relatively labile Schiff-base intermediates, and the more stable ketoamine products generated by Amadori rearrangement. Polyacrylamide gel electrophoresis was used to monitor protein aggregation; periodate oxidation, nuclear magnetic resonance (NMR), and oxalic acid hydrolysis combined with HPLC, emerged as the most promising methods to quantitate the degree of glycosylation. Possible interpretations are advanced to explain the apparent discrepancies in degree of glycosylation suggested by the different analytical methods evaluated.     

矩阵乘积的行式,列式

给出了ｍ×ｍ矩阵与ｍ×ｎ矩阵的行（列）式的表达式．若Ａ＝ａ１１ａ１２…ａ１ｍａ２１ａ２２…ａ２ｍ……ａｍ１ａｍ２…ａｍｍＢ＝ｂ１１ｂ１２…ｂ１ｎｂ２１ｂ２２…ｂ２ｎ……ｂｍ１ｂｍ２…ｂｍｎ分别是ｍ×ｍ，ｍ×ｎ矩阵，则｜Ａ｜｜Ｂ｜＝｜ＡＢ｜＋∑ｉ１＜ｉ２＜…＜ｉｔｊ１＜ｊ２＜…＜ｊｔ１≤ｔ≤ｍｎ－ｔ≥ｍＮＢｉ１ｉ２…ｉｔｊ１ｊ２…ｊｔＮＡＢ１…ｍ（－１）ｓｔ＋１ｊｔ＋１…（－１）ｓｎｊｎ其中ｉ１，ｉ２，…，ｉｔ是１，２，…，ｍ中ｔ个数码；ｊ１，ｊ２，…，ｊｔ，ｊｔ＋１，…，ｊｎ是１，２，…，ｎ的一个排列；ｓｒ＝π（ｊ１，ｊ２，…，ｊｔ，ｊｒ）（ｒ＝１，２，…，ｎ）是排列ｊ１，ｊ２，…，ｊｔ，ｊｒ的反序数．