Stimulatory effect of melanin-concentrating hormone on luteinising hormone release

We investigated the cytokine profile and peak levels of interleukin (IL) -6, IL-8, IL-10 and tumour necrosis factor (TNF) -alpha levels in 42 patients after allogeneic bone marrow transplantation (BMT). Eleven of them developed veno-occlusive disease (VOD) of the liver. Fourteen patients had moderate-to-severe acute graft-versus-host disease (aGvHD), 10 isolated bacteraemia and 7 had no major complication. Those who developed severe VOD (n=6) showed a short, very high IL-8 peak (median: 6632 pg/ml, range: 5546-10,000 vs. 280 pg/ml, 0-2042 in controls, p<0.01) 1-4 d after diagnosis of the liver disease. Five of these patients had high peak levels of IL-6. Five patients with mild VOD showed a lower increase in the cytokines tested. Bilirubin levels, at day of IL-8 peak, did not differ statistically between mild and severe VOD. The highest levels of IL-10 were found in those with aGvHD. IL-8 levels were also increased, but not to the same extent as in patients with severe VOD (p=0.01 vs. VOD). In patients with bacteraemia, very high levels of IL-6 were seen. In patients without major complications, the levels of cytokines were low. In conclusion, high levels of IL-8 occurred in severe VOD of the liver, which may be of value to determine prognosis.     

竖式烧结矿冷却塔内气固换热特性研究

通过搭建3.6 t/h竖式烧结矿冷却实验台,对烧结矿在下落过程中与空气的换热规律进行了研究,重点分析了不同气固比、冷却气体进口速度对气固换热过程的影响,并根据实验数据拟合出气固换热关联式。研究结果表明:随着气固比增加,冷却气体所携带的热量呈现出先增加后减小的趋势,当气固比在900~1 000 Nm3/t范围时,冷却气体携带的热量最大;随着冷却气体流速增加,气固换热系数不断增加,但其增长速率逐渐变慢;实验所得气固换热关联式为:Nu=1ε(17.67+1.26Re0.5),适用范围为:9 700≤Re≤25 000,0.80≤ε≤0.95,误差在±10%以内,具有一定的应用价值。     

Stimulatory effect of polyamines on polyphenylalanine synthesis on Mycobacterium smegmatis ribosomes

The augmented transposition flap plasty consists of a Z-plasty, to which a triangular flap from the dorsal ulnar aspect of the thumb is added. Remaining skin defects have to be covered by thick split thickness skin grafts. This procedure has been successfully during the past five years for the release of congenital or posttraumatic first web contractures of moderate severity.     

喷淋冷却塔在Consteel电炉上的应用

介绍喷淋冷却塔在Consteel电炉上的应用．     

无填料喷雾冷却塔的发展及改进思路

介绍了无填料喷雾冷却塔的发展过程，与传统的填料式冷却塔的结构和冷却效果进行比较，针对喷雾冷却塔结构上的缺点，提出几点改进思路。     

锌电积液空气冷却塔的改进实践

从电解车间空气冷却塔自身的结构特点出发,针对生产中存在的问题对其进行改造并使之性能优化。改造后塔体清理周期延长至半年,减少了酸雾逸出量,降低了对环境的污染,取得了较好的效果。     

喷雾式烟气排放冷却塔的设计方法

为提升烟气排放冷却塔后的集尘装置性能,大多企业已将以往使用的电除尘器更换为布袋除尘器。烟气冷却分为湿式冷却法和干式冷却法两种。采用干式冷却法不需要排放,也不需要进行排水处理,优点是喷雾水量少于湿式冷却法。介绍了干式冷却法的设计方法包括喷嘴的布置、风道实验和模拟测试方法。     

Stimulatory effect of N-methyl-D-aspartate on somatostatin gene expression in cultured hypothalamic neurons

The aim of the present study was to determine whether N-methyl-D-aspartate (NMDA) stimulates somatostatin gene function in primary cultures of hypothalamic neurons. Neurons were either shortly (for 3, 8, 24 and 72 h) or chronically (for 11 days) exposed to NMDA (20 microM). Medium and cellular somatostatin contents were determined by radioimmunoassay, and steady-state preprosomatostatin mRNA levels by Northern blot analysis with an oligonucleotide probe. DNA content was measured as a cellular viability control. After 8 h incubation, NMDA induced a significant 2-fold increase in somatostatin mRNA accumulation, with a maximal 4-fold increase after 24 h incubation. A significant and dose-dependent (1.7-fold and 2.5-fold at 20 and 100 microM, respectively) stimulatory effect was also observed after chronic treatment. The kinetic patterns for medium and cellular somatostatin contents were similar to those obtained for somatostatin mRNA levels. Total DNA content was not modified under any experimental condition. The augmentations in cellular somatostatin and somatostatin mRNA determined after 24 h or chronic exposure to NMDA were blocked by (+)-5-methyl-10.11-dihydro-5H-dibenzo(a,d')cyclohepten-5,10-imine hydrogen maleate (MK-801), an NMDA receptor antagonist. MK-801 alone significantly (P < 0.05) reduced somatostatin mRNA. The stimulatory effect of NMDA on somatostatin mRNA was specific since it was not accompanied by any change in glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA. After immunostaining with a specific antibody against somatostatin, no difference was observed in the number of immunostained neurons detected in control and NMDA exposed groups.(ABSTRACT TRUNCATED AT 250 WORDS)     

冷却水流量对大直径棒材冷却效果的影响规律

利用有限元耦合场数值模拟计算方法,对大直径棒材穿水冷却过程进行了三维非稳态数值模拟,研究了冷却水流量对冷却效果的影响规律,并分析了决定冷却水流量的两个因素——冷却器入口截面积及冷却水流流速对冷却效果的影响程度.模拟结果表明:当流量相对较小时,流量的改变对冷却效果的影响较大;当流量增大到一定值后,随着流量的继续增大,对冷却效果的影响逐渐减小;冷却水流流速对冷却效果的影响程度大于冷却器入口截面积.     

Stimulatory effect of low-power density He-Ne laser radiation on human fibroblasts in vitro

Cultures of human embryonic fibroblasts in vitro were subjected to helium-neon laser single and double irradiation to investigate the influence of low-energy laser irradiation on fibroblast proliferation. Mean increase in the cell number values of irradiated cells were compared with increase values of non-irradiated control samples of fibroblasts. He-Ne laser was used as a coherent source of monochromatic radiation at 632.8 nm, and Petri-dishes with cultured fibroblasts were irradiated in way to receive radiation of energy doses of 0.5; 1; 1.5 and 2J/cm2. Single He-Ne laser irradiation exhibited a significant stimulation effect on human fibroblast proliferation in cell-culture.     

Stimulatory effect of regucalcin on ATP-dependent calcium transport in rat liver plasma membranes

The effect of regucalcin, a calcium-binding protein isolated from rat liver cytoplasm, on ATP-dependent calcium transport in the plasma membrane vesicles of rat liver was investigated. (Ca(2+)- Mg2+)-ATPase activity in the liver plasma membranes was significantly increased by the presence of regucalcin (0.1-0.5 microM) in the enzyme reaction mixture. This increase was completely inhibited by the presence of sulfhydryl group modifying reagent Nethylmaleimide (5.0 mM NEM) or digitonin (0.04%), which can solubilize the membranous lipids. When ATP-dependent calcium uptake by liver plasma membrane vesicles was measured by using 45CaCl2, the presence of regucalcin (0.1-0.5 microM) in the reaction mixture caused a significant increase in the 45Ca2+ uptake. This increase was about 2-fold with 0.5 microM regucalcin addition. An appreciable increase was seen by 5 min incubation with regucalcin addition. The regucalcin-enhanced ATP-dependent 45Ca2+ uptake by the plasma membrane vesicles was completely inhibited by the presence of NEM (5.0 mM) or digitonin (0.04%). These results demonstrate that regucalcin activates (Ca(2+)-Mg2+)-ATPase in the liver plasma membranes and that it can stimulate ATP-dependent calcium transport across the plasma membranes.     

Stimulatory effect of lymphocyte-derived factor on catecholamine efflux from cultured bovine adrenal medullary cells

The effects of lymphocytes and their conditioned medium on catecholamine efflux and uptake were examined in cultured bovine adrenal medullary cells. Co-culture of adrenal medullary cells with lymphocytes for 3 days caused an increase in appearance of catecholamines in the culture medium. Treatment of adrenal medullary cells with a conditioned medium prepared from lymphocytes also enhanced the appearance of catecholamines in culture medium in time- (8-48 h) and concentration-dependent manners. Heat treatment of the conditioned medium at 60 and 100 degrees C for 10 min reduced its stimulatory effect to 59 and 20% of control, respectively. After gel filtration on a Sephadex G-25 column or dialysis (<8 kDa molecular mass cutoff), the stimulatory activity of the conditioned medium was found in a high molecular fraction. The conditioned medium had little effect on the activity of lactate dehydrogenase in the medium of cultured adrenal medullary cells and on desipramine-sensitive [3H]norepinephrine uptake by the cells. These findings suggest that lymphocytes release a heat-sensitive factor(s) (molecular mass of more than 8 kDa) which increases efflux of catecholamines from cultured adrenal medullary cells.     

韶钢4号高炉冷却塔工程地基处理及试验分析

韶钢4号高炉冷却塔工程,地质勘探表明,建设场地上层覆盖有5.1～7.0m厚的人工回填土,其承载力<130kPa.根据建筑荷载以及实际情况进行方案比选,采用了砂垫层换填法处理地基.经设计计算,砂垫层长、宽、厚分别为21.2、12.2和3.0m,并严格按现行有关规范进行施工.为了确保施工质量,砂垫层按照国标GBJ7-89附录四地基土载荷试验要点采用压重平台反力装置进行试验,结果表明:砂垫层累计沉降量为12.91～13.40mm,承载力基本值为240kPa,完全满足设计和使用要求,取得了缩短工期23d和节约工程投资20万元的经济效益.     

热装高炉高温煤气喷雾降温塔工艺计算

高炉原燃料热装工艺能显著提高铁前工序显热资源利用效率,为保证高温高炉煤气的净化回收系统安全,须在干法布袋除尘设施前设置喷雾蒸发降温塔。通过对干式喷雾蒸发降温理论进行分析,结合热装高炉操作变工况条件,推导出全套喷雾蒸发降温塔的理论计算方法。该方法可对塔内喷水量、降温塔出口煤气露点和含湿量、液滴蒸发时间、及塔体结构尺寸等关键技术参数进行便捷准确的计算,且易于计算机编程应用,对实际推广具有积极的意义。     

Stimulatory effect of saponin from Panax ginseng on immune function of lymphocytes in the elderly

In order to determine the epitope structure in peptide NP50-63, which has been reported to be the only Kk-restricted T-cell antigen within the influenza virus (A/PR/8/34) nucleoprotein, a series of 13 peptides truncated from C- and N-termini of NP50-63 were synthesized and their sensitizing activities against Kk-restricted nucleoprotein-specific cytotoxic T lymphocytes (CTLs) were examined. One of the 13 peptides, NP50-57, sensitized L929 cells at the nM level, which was 100-1000 times lower in concentration than that at which the other peptides sensitized these cells. The presence of NP50-57 in A/PR/8/34-infected L929 cells was also investigated. Acid extracts of virus-infected cells were separated on a reverse-phase HPLC column and then anion-exchange column. By both separations, only one peak of sensitizing activity against nucleoprotein-specific CTLs was observed. The position of the peak coincided with that of the elution of NP50-57. These results strongly suggest that NP50-57 is the natural epitope in the antigenic structure, NP50-63.     

Stimulatory effect of dihydroxyphenyl compounds on the aerotolerance of Spirillum volutans and Campylobacter fetus subspecies jejuni

Administration for different doses of cyproterone acetate (CPA) to male hamsters for 5 weeks slows down the growth of the adrenals and the increase in body weight. This effect is still present 4 weeks after cessation of CPA treatment, though some recovery can be seen. Histological and histometrical evaluations confirm an atrophy of the adrenal, particularly in the zona fasciculata and in higher doses also in the zona reticularis. After operative castration it is the zona reticularis which shows the most marked atrophic changes. It is therefore concluded, that CPA possesses besides its anti-androgenic properties an inhibiting corticoid-like effect on the adreno-pituitary feed-back mechanism.