共查询到19条相似文献,搜索用时 62 毫秒
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CYP1B1酶(Cytochrome P450 1B1,CYP1B1)是细胞色素P450酶(Cytochrome P450 enzyme, CYPs)的一个亚族,能参与多种内、外源性化合物的氧化/还原反应,在多种恶性肿瘤中高表达。CYP1B1酶逐渐成为抗肿瘤药物设计研发的热门靶点,许多学者以CYP1B1酶为靶标蛋白设计的抑制剂,表现出较好的活性和巨大的应用潜力,尤其是在克服肿瘤耐药性中具有重要意义。目前报道的CYP1B1抑制剂种类繁多,活性最佳的化合物是α-萘黄酮(ANF)。主要介绍了较为常见的几类CYP1B1抑制剂,简述了各类化合物的特点以及酶抑制效果,并对CYP1B1抑制剂的发展前景进行了展望。 相似文献
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《化学试剂》2021,43(3):365-369
以一种柔性紫精衍生物1,1′-双(4-羧苄基)-4,4′-联吡啶二氯化物为配体,合成一种新型联吡啶羧酸铜配合物{[Cu(Bpybc)_(1.5)(H_2O)_2]·SO_4}_n,对其结构和组成进行红外光谱、X-射线单晶衍射和元素分析表征。该配合物属于三方晶系,空间群R-3,晶胞参数为:a=17.823 2(14)?,b=17.823 2(14)?,c=26.987(2)?,α=90°,β=90°,γ=120°,V=7 424.3(13)?~3,Z=6,R_(int)=0.134 7,R_1=0.095 0,T=293(2) K。此外,通过体外抑制活性实验研究了该配合物对蛋白酪氨酸磷酸酶1B(PTP1B)的抑制作用,该配合物能够有效抑制PTP1B的活性,其IC_(50)值为0.095μmol/L。 相似文献
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以藜芦醛(Ⅰ)为起始原料,经过溴代、还原、傅克烷基化、脱甲基4步反应,合成了化合物4,5-二-(2-溴-4,5-二羟基苯甲基)-1,2-二苯酚(Ⅵ),总产率为53.5%。采用1HNMR、13CNMR、HREIMS等进行了结构表征。通过比色法对化合物Ⅵ及中间产物4,5-二-(2-溴-4,5-二甲氧基苯甲基)-1,2-二甲氧基苯(Ⅴ)进行蛋白酪氨酸磷酸酶1B(protein tyrosine phosphatase 1B,PTP1B)抑制活性测定,结果显示化合物质量浓度为20 mg/L时,化合物Ⅴ和Ⅵ的PTP1B酶抑制率分别为25.08%和79.48%,表明化合物Ⅵ具有较好的PTP1B酶抑制活性。 相似文献
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目的建立同时测定五维B颗粒中维生素B1、维生素B2、烟酰胺三组分含量的高效液相色谱法.方法采用ODS C18柱、柱长150×4.6mm流动相为甲醇乙腈0.06%己烷磺酸钠和0.04%庚烷磺酸钠的混合液冰醋酸=4530241流速1.2mL/min柱温30℃波长275nm,外标法计算.结果线性范围分别是烟酰胺90~210μg/mL;r=0.999维生素B2 12~32μg/mL;r=0.999、维生素B1 25~65μg/mL;r=0.998,回收率分别为烟酰胺99.5%、维生素B2 99.7%、维生素B1 100.3%.结论本方法分离效果好、辅料无干扰、快速、简便、准确适合于该制剂3组分的同时测定. 相似文献
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以奇瑞商用车河南开封30JPH涂装线为例,介绍了水性漆B1B2工艺喷漆室、色漆闪干、流平段及面漆烘干室体的整体工艺规划及设备布局,对各工艺段的长度、参数、设备应用、材料选择与应用等进行了介绍. 相似文献
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Xiangrong Chen Mohamed F. Abdallah Charlotte Grootaert Andreja Rajkovic 《International journal of molecular sciences》2022,23(13)
Fumonisin B1 (FB1) and aflatoxin B1 (AFB1) are frequent contaminants of staple foods such as maize. Oral exposure to these toxins poses health hazards by disrupting cellular signaling. However, little is known regarding the multifaced mitochondrial dysfunction-linked toxicity of FB1 and AFB1. Here, we show that after exposure to FB1 and AFB1, mitochondrial respiration significantly decreased by measuring the oxygen consumption rate (OCR), mitochondrial membrane potential (MMP) and reactive oxygen species (ROS). The current work shows that the integrity of mitochondria (MMP and ROS), that is the central component of cell apoptosis, is disrupted by FB1 and AFB1 in undifferentiated Caco-2 and HepG2 cells as in vitro models for human intestine and liver, respectively. It hypothesizes that FB1 and AFB1 could disrupt the mitochondrial electron transport chain (ETC) to induce mitochondrial dysfunction and break the balance of transferring H+ between the mitochondrial inner membrane and mitochondrial matrix, however, the proton leak is not increasing and, as a result, ATP synthesis is blocked. At the sub-toxic exposure of 1.0 µg/mL for 24 h, i.e., a viability of 95% in Caco-2 and HepG2 cells, the mitochondrial respiration was, however, stimulated. This suggests that the treated cells could reserve energy for mitochondrial respiration with the exposure of FB1 and AFB1, which could be a survival advantage. 相似文献
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以阿维菌素和叔丁基二甲基硅基氯为原料合成 5 O 叔丁基二甲基硅基阿维菌素。其优惠工艺条件为 :反应温度 2 0℃ ,反应时间 2h,阿维菌素∶叔丁基二甲基硅基氯 =1∶3(mol/mol) ,产品收率≥ 90 % ,含量≥ 82 %。 相似文献
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Islam M. Y. Abdellatif Shaoze Yuan Renhu Na Shizue Yoshihara Haruyasu Hamada Takuya Suzaki Hiroshi Ezura Kenji Miura 《International journal of molecular sciences》2022,23(3)
Heat stress (HS) is a prevalent negative factor affecting plant growth and development, as it is predominant worldwide and threatens agriculture on a large scale. PHYTOCHROMES (PHYs) are photoreceptors that control plant growth and development, and the stress signaling response partially interferes with their activity. PHYA, B1, and B2 are the most well-known PHY types in tomatoes. Our study aimed to identify the role of tomato ‘Money Maker’ phyA and phyB1B2 mutants in stable and fluctuating high temperatures at different growth stages. In the seed germination and vegetative growth stages, the phy mutants were HS tolerant, while during the flowering stage the phy mutants revealed two opposing roles depending on the HS exposure period. The response of the phy mutants to HS during the fruiting stage showed similarity to WT. The most obvious stage that demonstrated phy mutants’ tolerance was the vegetative growth stage, in which a high degree of membrane stability and enhanced water preservation were achieved by the regulation of stomatal closure. In addition, both mutants upregulated the expression of heat-responsive genes related to heat tolerance. In addition to lower malondialdehyde accumulation, the phyA mutant enhanced proline levels. These results clarified the response of tomato phyA and phyB1B2 mutants to HS. 相似文献
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以苯胺和顺丁烯二酸酐为原料,维生素B1为催化剂,醋酸酐为脱水剂,在丙酮溶剂中采用两步法(醋酐法)合成N-苯基马来酰亚胺。通过对催化剂浓度、反应时间以及脱水剂醋酸酐与顺丁烯二酸酐的配比等工艺条件进行优化,实验结果表明,维生素B1有着很好的催化活性,脱水剂乙酸酐与顺丁烯二酸酐配比为1.6∶1时,催化剂浓度为1.5%(以顺丁烯二酸酐质量计)时,反应时间为4h时为最佳反应条件。在最佳反应条件下,产率可达到63.2%。对丙酮溶剂进行重复使用考察,结果证明溶剂重复使用性好。 相似文献
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Janik Riese Alina Gromann Felix Lührs Annabel Kleinwort Tobias Schulze 《International journal of molecular sciences》2021,22(7)
Background: Gram-negative infections of the peritoneal cavity result in profound modifications of peritoneal B cell populations and induce the migration of peritoneal B cells to distant secondary lymphoid organs. However, mechanisms controlling the egress of peritoneal B cells from the peritoneal cavity and their subsequent trafficking remain incompletely understood. Sphingosine-1-phosphate (S1P)-mediated signaling controls migratory processes in numerous immune cells. The present work investigates the role of S1P-mediated signaling in peritoneal B cell trafficking under inflammatory conditions. Methods: Differential S1P receptor expression after peritoneal B cell activation was assessed semi‑quantitatively using RT-PCR in vitro. The functional implications of differential S1P1 and S1P4 expression were assessed by transwell migration in vitro, by adoptive peritoneal B cell transfer in a model of sterile lipopolysaccharide (LPS)‑induced peritonitis and in the polymicrobial colon ascendens stent peritonitis (CASP) model. Results: The two sphingosine-1-phosphate receptors (S1PRs) expressed in peritoneal B cell subsets S1P1 and S1P4 are differentially regulated upon stimulation with the TLR4 agonist LPS, but not upon PMA/ionomycin or B cell receptor (BCR) crosslinking. S1P4 deficiency affects both the trafficking of activated peritoneal B cells to secondary lymphoid organs and the positioning of these cells within the functional compartments of the targeted organ. S1P4 deficiency in LPS-activated peritoneal B cells results in significantly reduced numbers of splenic innate response activator B cells. Conclusions: The S1P-S1PR system is implicated in the trafficking of LPS-activated peritoneal B cells. Given the protective role of peritoneal B1a B cells in peritoneal sepsis, further experiments to investigate the impact of S1P4-mediated signaling on the severity and mortality of peritoneal sepsis are warranted. 相似文献
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Rongxing Liu Ccile Mathieu Jrmy Berthelet Wenchao Zhang Jean-Marie Dupret Fernando Rodrigues Lima 《International journal of molecular sciences》2022,23(13)
Phosphorylation is an essential process in biological events and is considered critical for biological functions. In tissues, protein phosphorylation mainly occurs on tyrosine (Tyr), serine (Ser) and threonine (Thr) residues. The balance between phosphorylation and dephosphorylation is under the control of two super enzyme families, protein kinases (PKs) and protein phosphatases (PPs), respectively. Although there are many selective and effective drugs targeting phosphokinases, developing drugs targeting phosphatases is challenging. PTP1B, one of the most central protein tyrosine phosphatases (PTPs), is a key player in several human diseases and disorders, such as diabetes, obesity, and hematopoietic malignancies, through modulation of different signaling pathways. However, due to high conservation among PTPs, most PTP1B inhibitors lack specificity, raising the need to develop new strategies targeting this enzyme. In this mini-review, we summarize three classes of PTP1B inhibitors with different mechanisms: (1) targeting multiple aryl-phosphorylation sites including the catalytic site of PTP1B; (2) targeting allosteric sites of PTP1B; (3) targeting specific mRNA sequence of PTP1B. All three types of PTP1B inhibitors present good specificity over other PTPs and are promising for the development of efficient small molecules targeting this enzyme. 相似文献