云南文山三七须根急性毒性和诱变毒性作用的研究

目的 探讨云南文山三七须根急性毒性和诱变毒性作用。方法 急性毒性采用一次限量法, 剂量设为10000 mg/kg BW。Ames实验使用的菌株为鼠伤寒沙门氏菌组氨酸缺陷型菌株TA97、TA98、TA100、TA102。实验分别设每皿5000、1000、200、40、8 μg剂量组, 同时设阴性、阳性对照组, 采用平板掺入法, 观察每皿回变菌落数。微核及精子畸形试验均使用SPF级昆明种小鼠, 实验设1250、2500、5000 mg/kg BW 3个剂量组、一个阴性对照组及一个阳性对照组。微核试验采用30 h给受试物法, 检测骨髓嗜多染红细胞微核率。精子畸形试验于首次染毒后第35 d处死动物, 观察精子畸形率。结果 云南文山三七须根对受试动物的急性毒性(LD50)>10000 mg/kg BW。与阴性对照组比较, 云南文山三七须根各剂量组在鼠伤寒沙门氏菌试验(Ames试验)中实验结果为阴性, 骨髓细胞微核试验表明未引起微核发生率增加, 对小鼠精子畸变无明显影响(P＞0.05)。结论 云南文山三七须根对昆明种小鼠的急性经口毒性属实际无毒, 在Ames实验中未呈现致突变作用, 未见诱导小鼠骨髓嗜多染红细胞微核形成的能力, 未见有致小鼠精子畸形作用。     

一种无溶剂饮水舱防腐涂料的毒性研究

目的 探讨一种无溶剂饮水舱防腐涂料的毒性作用。方法 急性经口毒性试验采用啮齿类动物急性固定剂量经口毒性试验一次限量法, 剂量设为20000mg/kgBW。微核试验设5000、2500、1250mg/kgBW 3个剂量组、阴性对照组及阳性对照组,采用30h给受试物法,检测骨髓嗜多染红细胞微核率。Ames试验分别设每皿200、100、40、10μl共四个剂量组,同时设阴性、阳性对照组,采用平板掺入法,观察每皿回变菌落数。结果 该种无溶剂饮水舱防腐涂料对受试动物的急性毒性(LD₅₀)＞20000mg/kgBW。与阴性对照组比较,骨髓细胞微核试验结果为阴性。三七须根各剂量组在Ames试验中未呈现致突变性。结论 在本实验条件和剂量范围内,该种无溶剂饮水舱防腐涂料对昆明种小鼠的急性经口毒性属实际无毒,未见诱导小鼠骨髓嗜多染红细胞微核形成的能力,在Ames实验中未见呈现致突变作用。     

内消旋玉米黄素的遗传毒性研究

目的:评价内消旋玉米黄素的遗传毒性,为其作为食品原料和食品添加剂应用提供毒理学依据。方法:采用Ames试验、小鼠骨髓嗜多染红细胞微核试验和小鼠精子畸形试验,评价内消旋玉米黄素的遗传毒性。内消旋玉米黄素Ames试验选用TA97、TA98、TA100和TA102四种测试菌株,试验设5000、1000、200和40μg/皿4个剂量组,并设阳性对照和自发回复突变对照;内消旋玉米黄素小鼠骨髓细胞微核试验,设1250、2500和5000mg/kg 3个给药组、阴性对照组和阳性对照组(环磷酰胺60mg/kg)。内消旋玉米黄素小鼠精子畸形试验,设1250、2500和5000mg/kg 3个给药组、阴性对照组和阳性对照组(丝裂霉素C 2.0 mg/kg)。结果:在不大于5000μg/皿剂量范围内,加入和不加入S9的内消旋玉米黄素各剂量组对TA97、TA98、TA100和TA102四种菌株的回变菌落数与阴性对照组比较差异均无统计学意义(P0.05),且无剂量-反应关系;在1250~5000mg/kg剂量范围内,内消旋玉米黄素小鼠骨髓嗜多染红细胞微核试验及小鼠精子畸形试验结果均为阴性。结论:在试验剂量范围内,内消旋玉米黄素未显示遗传毒性作用。     

红曲提取物的急性毒性和致突变性研究

研究红曲提取物的急性毒性和突变性.采用最大耐受剂量法研究急性毒性;采用鼠伤寒沙门氏菌回复突变试验(Ames试验)、骨髓细胞微核试验和小鼠精子畸形试验研究其致突变性.昆明种小鼠以最大给药量为15 g/kg灌胃,小鼠无明显中毒症状亦无死亡;Ames试验选用TA97、TA98、TA100、TA102四种菌株在加与不加S.两种情况下试验研究,结果显示为诱变阴性;小鼠骨髓细胞微核试验3个剂量组的微核发生率与阴性对照组比较无显著性差异;小鼠精子畸形试验中试验组的精子异常率与阴性对照组相比亦无明显差异.红曲提取物的毒性级别为无毒级;在试验条件下,未发现其有致突变性.     

盐肤木油致突变性研究

根据《食品安全性毒理学评价程序与方法》要求,采用小鼠骨髓细胞微核试验、小鼠精子畸形试验及Ames试验研究盐肤木油的致突变性.结果表明:盐肤木油各剂量组对小鼠骨髓细胞微核试验为阴性(P＞0.05),对小鼠精子无致畸作用(P＞0.05);对Ames试验中的TA97、TA98、TA100、TA102未显示致突变性.说明在试验条件下,盐肤木油无致突变作用.     

黑曲霉电子束辐照突变菌的遗传毒性评价

依据食品安全性评价中遗传毒性评价的方法,通过对电子束辐照诱变育种黑曲霉突变菌进行Ames试验、小鼠骨髓细胞微核试验、小鼠精子畸形试验研究,评价黑曲霉电子束辐照突变菌的遗传毒性。结果表明,在Ames试验中,黑曲霉突变菌各剂量组无论是否添加S9代谢活化系统,黑曲霉突变菌各剂量组的回变菌落数均未超过自发回变菌落数的2倍,表明黑曲霉突变菌对染色体无致突变作用。在小鼠骨髓细胞微核试验中,黑曲霉突变菌不能引起哺乳动物嗜多染红细胞的微核细胞率显著增加(p>0.05),黑曲霉突变菌对细胞染色体并未造成明显损伤,表明黑曲霉突变菌并无致突变作用。在小鼠精子畸形试验中,黑曲霉突变菌各剂量组的精子畸形率与阴性对照组相比无显著性差异(p>0.05),表明黑曲霉突变菌并未对小鼠的精子产生致畸作用。因此,在本实验条件下,Ames试验、小鼠骨髓细胞微核试验、小鼠精子畸形试验结果均为阴性。表明黑曲霉电子束辐照突变菌属于实际无毒级别,并无遗传毒性作用。     

薏苡仁油致突变性实验研究

按照《保健食品检验与评价技术规范》,采用Ames试验、小鼠骨髓细胞微核试验和小鼠精子畸形试验研究薏苡仁油的致突变性。结果表明:Ames试验、小鼠骨髓细胞微核试验和小鼠精子畸形试验结果均为阴性。因此,在本试验条件下,薏苡仁油未显示有致突变性。     

丹凤牡丹花遗传毒理学研究

目的通过3项致突变实验,了解丹凤牡丹花的遗传毒性,并对其安全性做出评价。方法骨髓细胞微核试验:通过2次经口灌胃小鼠后观察计数含微核PCE数。Ames试验:利用试验菌株的鼠伤寒沙门氏菌,氨酸缺陷型4株进行试验,最终计数各皿回变菌落数。小鼠精子畸形试验:给予试验样品每日灌胃小鼠,连续5d,末次灌胃后30d计数畸形精子数。结果骨髓细胞微核试验、Ames试验、小鼠精子畸形试验结果均为阴性。结论丹凤牡丹花遗传毒性无毒,安全性较高。     

辅酶Q10软胶囊的急性毒性和遗传毒性研究

目的:研究辅酶Q10软胶囊的急性毒性和遗传毒性,为辅酶Q10的进一步开发利用提供安全依据。方法:应用小鼠急性经口毒性试验、Ames试验、小鼠骨髓嗜多染红细胞微核试验和小鼠精子畸形试验对某种辅酶Q10软胶囊的毒性进行试验观察。结果:该辅酶Q10软胶囊对雌雄小鼠LD50均大于10 000mg/kg.bw,属实际无毒物质;Ames试验中受试物各剂量组回变菌落数均未超过自发回变菌落数的2倍,亦无剂量-反应关系,结果为阴性;小鼠骨髓嗜多染红细胞微核试验和小鼠精子畸形试验中各剂量组微核率、精子畸形率与阴性对照组之间无显著性差异(P0.05),而环磷酰胺阳性对照组与阴性对照组之间有极显著性差异(P0.01),证明试验结果均为阴性。结论:本次试验条件下,该辅酶Q10软胶囊属实际无毒级,未显示有急性毒性和遗传毒性作用。     

一种微生物絮凝剂急性毒性和诱变毒性作用的研究

目的研究一种微生物絮凝剂受试物的急性毒性和诱变毒性作用。方法分别对微生物絮凝剂进行了小鼠急性经口毒性试验、Ames试验、小鼠骨髓嗜多染红细胞微核试验。结果该微生物絮凝剂的小鼠急性经口毒性试验LD_(50)≥5000 mg/(kg BW);Ames试验中,各剂量组回复突变菌落数均未见明显增加,亦无剂量-反应关系;小鼠骨髓嗜多染红细胞微核试验中,微生物絮凝剂各剂量组微核细胞率与阴性对照组比较差异无显著性。结论该微生物絮凝剂对昆明种小鼠的急性经口毒性属实际无毒,在Ames试验中未呈现致突变作用,未见诱导小鼠骨髓嗜多染红细胞微核形成的能力。     

Reliable identification of grapevine rootstock varieties using RAPD PCR on woody samples

Since grapevine ( Vitis spp .) rootstock material is being traded increasingly as disbudded woody material a lack of distinctive morphological features on such material necessitates an alternative and reliable means of identification. Methods described here were developed for rapid and efficient extraction of DNA from woody samples rich in phenolic compounds and polysaccharides, and for subsequent identification of varieties by RAPD PCR. Using these methods, and with the application of only one selected RAPD primer, we were able to differentiate sixteen rootstock varieties, including the seven varieties most commonly used in Germany. Problems commonly encountered with reproducibility of RAPD patterns were avoided by choosing primers with a dinucleotide sequence and a high G/C content that allowed a rather high annealing temperature of 45°C. Methods described here should also be useful for other horticultural crops, especially those with woody tissues rich in phenolic compounds and polysaccharides.     

Aroma characterization of various apricot varieties using headspace–solid phase microextraction combined with gas chromatography–mass spectrometry and gas chromatography–olfactometry

The characterization of the aromatic profile of several apricot cultivars with molecular tracers in order to obtain objective data concerning the aromatic quality of this fruit was undertaken using headspace–solid phase microextraction (HS–SPME). Six apricot cultivars were selected according to their organoleptic characteristics: Iranien, Orangered, Goldrich, Hargrand, Rouge du Roussillon and A4025. The aromatic intensity of these varieties measured by HS–SPME–Olfactometry were defined and classified according to the presence and the intensity of grassy, fruity and apricot like notes. In the six varieties, 23 common volatile compounds were identified by HS–SPME–GC–MS. Finally, 10 compounds, ethyl acetate, hexyl acetate, limonene, β-cyclocitral, γ-decalactone, 6-methyl-5-hepten-2-one, linalool, β-ionone, menthone and (E)-hexen-2-al were recognized by HS–SPME–GC–O as responsible of the aromatic notes involved in apricot aroma and considered as molecular tracers of apricot aromatic quality which could be utilized to discriminate apricot varieties.     

Tests of potential functional barriers for laminated multilayer food packages. Part I: Low molecular weight permeants

The advent of the functional barrier concept in food packaging has brought with it a requirement for fast tests of permeation through potential barrier materials. In such tests it would be convenient for both foodstuffs and materials below the functional barrier (sub-barrier materials) to be represented by standard simulants. By means of inverse gas chromatography, liquid paraffin spiked with appropriate permeants was considered as a potential simulant of sub-barrier materials based on polypropylene (PP) or similar polyolefins. Experiments were performed to characterize the kinetics of the permeation of low molecular weight model permeants (octene, toluene and isopropanol) from liquid paraffin, through a surrogate potential functional barrier (25 μm-thick oriented PP) into the food simulants olive oil and 3% (w/v) acetic acid. These permeation results were interpreted in terms of three permeation kinetic models regarding the solubility of a particular model permeant in the post-barrier medium (i.e. the food simulant). The results obtained justify the development and evaluation of liquid sub-barrier simulants that would allow flexible yet rigorous testing of new laminated multilayer packaging materials.     

Analysis of benzo[a]pyrene in spiked fatty foods by second derivative synchronous spectrofluorimetry after microwave-assisted treatment of samples

A simple, rapid and inexpensive method has been developed for the determination of benzo[a     

The development of reference materials for paralytic shellfish poisoning toxins in lyophilized mussel. II: Certification study

This paper describes the second part of a project undertaken to develop certified mussel reference materials for paralytic shellfish poisoning toxins. In the first part two interlaboratory studies were undertaken to investigate the performance of the analytical methodology for several PSP toxins, in particular saxitoxin and decarbamoyl-saxitoxin in lyophilized mussels, and to set criteria for the acceptance of results to be applied during the certification exercise. Fifteen laboratories participated in this certification study and were asked to measure saxitoxin and decarbamoyl-saxitoxin in rehydrated lyophilized mussel material and in a saxitoxin-enriched mussel material. The participants were allowed to use a method of their choice but with an extraction procedure to be strictly followed. The study included extra experiments to verify the detection limits for both saxitoxin and decarbamoyl-saxitoxin. Most participants (13 of 15) were able to meet all the criteria set for the certification study. Results for saxitoxin.2HCl yielded a certified mass fraction of <0.07 mg/kg in the rehydrated lyophilized mussels. Results obtained for decarbamoyl-saxitoxin.2HCl yielded a certified mass fraction of 1.59+/-0.20 mg/kg. The results for saxitoxin.2HCl in enriched blank mussel yielded a certified mass fraction of 0.48 +/- 0.06 mg/kg. These certified reference materials for paralytic shellfish poisoning toxins in lyophilized mussel material are the first available for laboratories to test their method for accuracy and performance.     

Announcing a new international peer-reviewed journal:Food Science and Human Wellness now accepting manuscript submission in English

<正>We are pleased to announce the launch of a new international peer-reviewed journal-Food Science and Human Wellness,ISSN 2213-4530,which is an open access journal,produced and hosted by Elsevier B.V.on behalf of Beijing Academy of Food Sciences.Food Science and Human Wellness is an international peer-reviewed English journal that provides a forum for the dissemination of the     

Chinese CTP Market

According to Printing and Printing Equipment Industries Association of China（PEIAC）＇s statistics to the plate manufucturer in China, in 2013, the actual offset plate production has reached 346 million square meters in China. Among them, the CTP production volume was 245 million square meters, up by 11% than that of last year; the total sales of the CTP plate was 239 million square meters, up by 13%.     

Preparatory Work of Print China 2015 is Going Smoothly

正Held at Guangdong Modern International Exhibition Center,Print China 2015 will cover 7exhibition halls,besides the original Hall No.3,4,5,6,7,the newly built F zone of Hall 3 will be used too.The total area will be140,000 square meters.Hall 3:Offset and large printing equipment,package printing equipment,post press     

INFOPRINT 2014 was Successfully Held in Dongguan

Sponsored by Printing and Printing Equipment Industries Association of China （PEIAC） and organized by Print China magazine, the Seventeenth Beijing International Printing Information Conference （INFOPRINT 2014） was successfully held on 11th Dec. 2014 at Dongguan Exhibition International Hotel.