The effect of selected hydrocolloids on the rheological properties of processed cheese analogues made with vegetable fats during the cooling phase

The aim of this work was to observe the influence of different hydrocolloids on changes in the rheological properties of processed cheese and their analogues within the process of cooling depending on the type of the fat used (butter, coconut fat and palm oil). κ‐carrageenan, ι‐carrageenan, λ‐carrageenan, arabic gum and locust bean gum were used at a concentration of 1.0% w/w. With the decreasing temperature during the cooling period, an increase in the complex modulus (G*) was observed in all samples tested. Within the cooling period, the sample with the addition of κ‐carrageenan showed both the most significant increase in G* in comparison with the control and the highest values of hardness after a 7‐day storage period regardless of the type of the fat used. The samples with coconut fat were assessed as the hardest. On the other hand, the samples with palm oil showed the lowest hardness (with the same hydrocolloid used). In the gelling hydrocolloids (κ‐ and ι‐carrageenan), only small changes in temperature of coil‐to‐helix transition were observed (in range of 2–7 °C) as a result of the addition of different types of fat.     

Influence of the casein/fat ratio in milk on the moisture in the non-fat substance in Cheddar cheese

For maximum profitability, the moisture in the non-fat substance (MNFS) in Cheddar cheese must be controlled. Commercial data are used to show that, with fixed manufacturing conditions, the MNFS in cheese may be increased by decreasing the casein/fat ratio in milk and vice versa. It is suggested that this method of varying MNFS may be useful in factories that have difficulty in regulating MNFS by other methods. The relationships between the casein/fat ratio in milk, manufacturing conditions and the fat in the dry matter, MNFS, fat and moisture percentages in cheese are explained.     

Composition,structure, rheological properties,and sensory texture of processed cheese analogues

Processed cheese analogues were made with different fat and moisture in nonfat solids (MNFS) contents and used to study relationships between composition, microscopic structure, rheological properties and sensory texture. Fat contents ranged from 58 to 230 g kg^?1 and MNFS from 487.6 to 603.3 g kg^?1. Regressions showed that those rheological parameters measured by uniaxial compression at large deformations and moderately high rates were best explained by linear combinations of MNFS, protein and fat. The moisture in the protein network acted as a plasticiser lowering the rheological parameters. Fat alone had little direct effect on the rheological characteristics under these conditions but at small deformations and low deformation rates it formed elastic inclusions that contributed to the properties. Examination of fracture surfaces in the SEM suggested that, during fracture, fat was squeezed out and acted as a lubricant. Maximum stress and work to maximum stress measured at moderately high deformation rates related best to the sensory results and described fracture of the cheeses. From the regressions, for 95% confidence limits, sensory analysis could theoretically measure changes within the range of composition tested of <0.1% in MNFS or fat. Of the rheological parameters only work to maximum stress was as sensitive. However, interaction between these compositional factors reduced the sensitivity in practice.     

Soya bean oil/soya protein isolate and carrageenan emulsions as fat replacer in fat‐reduced Oaxaca‐type cheese

Milk fat was replaced in Oaxaca‐type cheese with soya bean oil emulsions stabilised with soya protein isolate and different carrageenans (kappa, iota and lambda). Inclusion of soya bean oil emulsion increased yield, moisture and protein content and reduced fat content. Fat reduction and moisture incorporation provoked a tougher but less ductile texture. Specific interaction of carrageenans with milk proteins resulted in larger spreading area when melted and a softer and more adhesive texture, particularly in samples containing lambda‐carrageenan. These results are useful to improve the nutritional composition of Oaxaca‐type cheese, allowing the control of textural properties during cheese melting.     

Improvement of emulsifying properties of milk proteins with κ or λ carrageenan: effect of pH and ionic strength

The objective of this work was to determine the effect of λ‐carrageenan or κ‐carrageenan on the emulsion capacity, emulsion work and emulsion stability of milk proteins concentrate (MP) or sodium caseinate (SC) emulsions at different levels of pH and ionic strength. Incorporation of carrageenans to proteins emulsions resulted in an improvement of emulsifying properties at pH 6.0 and low ionic strength (0.2 m NaCl). Although emulsion capacity was high in MP than for SC, irrespectively of carrageenan employed, addition of λ‐carrageenan increased twofold emulsion work values (15 327 Ω s^?1 for MP and 11 455 Ω s^?1 for SC; around 6000 Ω s^?1 in the other treatments). Emulsion stability was high with λ‐carrageenan (9.8 s) than MP‐κ‐carrageenan or MP (7.45 and 7.40 s, respectively). Carrageenan improving of emulsion properties was because of the complex formation with MP, characteristic of this type of food system when pH was above of isoelectric point.     

The effects of lipase‐encapsulating carriers on the accelerated ripening of Kashar cheese

In this study, lipase enzymes were encapsulated in κ‐carragenan, gellan and sodium alginate using emulsion and extrusion techniques and were then added to cheese milk together with rennet. The effects of the encapsulating material and ripening period on the chemical, textural and sensory characteristics of Kashar cheese were investigated. The study demonstrated that sodium alginate, gellan and κ‐carrageenan could successfully be used as lipase carrier systems to accelerate the fat breakdown process during the ripening of Kashar cheese. Those samples treated with κ‐carrageenan capsules showed the highest rate of lipolysis and proteolysis compared to those treated with the other capsules.     

Low‐fat Cheddar cheese made using microparticulated whey proteins: Effect on yield and cheese quality

Influence of different levels (0, 0.15, 0.35 or 0.50%) of microparticulated whey protein (MWP) on yield and quality of low‐fat (~7.3 g/100 g) Cheddar cheese was investigated. MWP improved cheese yield due to the water‐binding ability of denatured whey protein. MWP addition decreased meltability but improved the textural properties beneficial for shredding and slicing, by decreasing sensory firmness. The results emphasise the role of MWP as an inert filler within cheese matrix, in improving cheese yield and creating a softer texture without compromising the sensory or overall quality of cheese, even with moisture increases in 0.35 or 0.50% MWP cheeses.     

Emulsifying properties of ovalbumin in mixtures with sulphated polysaccharides: effects of pH,ionic strength,heat and high‐pressure treatment

The influence of sulphated polysaccharides (dextran sulphate (DS), ι‐carrageenan (ι‐CAR) and κ‐carrageenan (κ‐CAR)) on the emulsifying properties of ovalbumin (OVA) has been investigated over a range of pressures and temperatures. Oil‐in‐water emulsions (10 g l⁻¹ protein, 200 ml l⁻¹ n‐tetradecane, pH 6.2) prepared with heat‐treated (80 °C for 10 min) mixtures of OVA + DS (1:0.25 by weight) had increased creaming stability but unchanged average droplet size compared to those made with untreated OVA and OVA + DS. Emulsions made with pressure‐processed (600 MPa for 20 min) OVA + DS (1:0.25 or 1:0.5 by weight) mixtures had the best emulsifying efficiency and stability. Under similar experimental conditions, replacement of DS with either ι‐ or κ‐CAR gave emulsions with larger droplets and more rapid serum separation, probably owing to depletion flocculation. High‐pressure treatment (600 MPa) of the OVA and mixed biopolymer solutions at pH 6.2 in the presence of salt (>0.04 M ) led to unstable emulsions, and so the protective effect of DS was lost. High‐pressure treatment (600 MPa) of emulsions prepared with native OVA or OVA + DS mixtures induced significant levels of flocculation, as indicated by changes in the average droplet size and creaming behaviour. © 2000 Society of Chemical Industry     

Influence of carboxymethylcellulose on the physicochemical,rheological and sensory attributes of a low‐fat Domiati cheese

The physicochemical, rheological and sensory attributes of a low‐fat Domiati cheese produced using carboxymethylcellulose (CMC), a hydrocolloid, at 0.4, 0.6, 0.8 and 1% (w?w) were examined during the ripening period. Results indicated that, as the carboxymethylcellulose content of cheese milk increased, cheese yield and moisture of low‐fat Domiati cheese significantly increased but the protein, salt and fat values significantly decreased. Rheological parameters were significantly lower in cheeses made with CMC. With regard to the sensory properties of the cheeses, low‐fat Domiati cheese made with 1% (w?w) CMC recorded the highest scores for sensory attributes.     

Instron texture profile of buffalo milk cheddar cheese as influenced by composition and ripening changes

Buffalo milk Cheddar cheese samples of different ages were analysed for compositional attributes (CA), ripening indices (RI) and Instron Textural Profile (ITP). All samples were compositionally alike, except for pH and salt-in-moisture (SM) contents. RI showed significant variations. CA and RI showed highly significant correlations within themselves and with each other, except for moisture with pH, SM with moisture, MNFS, Fat and FDM and Fat with MNFS. The ITPs of cheeses showed significant variations and had highly significant intercorrelations indicating their interdependence. CA (except moisture and MNFS) and RI showed a highly significant correlationship with ITPs. Moisture content showed a highly significant correlationship with all ITPs, except cohesiveness and springiness, where it was significant. MNFS content showed significant correlations only with hardness and brittleness. Stepwise regression analysis revealed that MI was the most predominant factor influencing cheese texture, followed by pH, SM, FDM and TVFA. Knowing Ca and RI, the textural properties of cheeses can be forecast through mathematical equations. Similarly the age of cheese can also be predicted if RI and/or textural properties are known.     

Physicochemical,textural and sensory properties of white soft cheese made from buffalo and cow milk mixtures

The physicochemical, textural and sensory properties of white soft cheeses made from three different buffalo and cow milk mixtures (100:0, 70:30 and 30:70) during 3‐month storage were studied. The increase in buffalo milk concentration resulted in increasing total cheese yield, dry matter (DM) and fat retention and fat in DM content. However, it caused reductions in moisture content, salt intake, hardness, chewiness, elasticity, sensory hardness and sensory cohesiveness of the samples. The percentage of water‐soluble nitrogen to total nitrogen increased during storage resulting in decreased fracturability, hardness (textural and sensory), cohesiveness (textural and sensory), springiness, chewiness and elasticity. The panellists evaluated the white soft cheese made with buffalo milk as the most acceptable.     

Effect of Lecithin on Cheese Yield

Cheese manufactured from milk containing three types of lecithin with different acetone-insoluble concentrations were compared with control cheese. A randomized block design with four treatments (three lecithins and one control) was replicated six times in the manufacture of 24 vats of cheese. Commercial lecithins (.05%) were added to the cheese milk at the time of starter addition. Cheese was manufactured by a Colby procedure. Milk was assayed for total solids, fat, total nitrogen, noncasein nitrogen, and acid degree value. Cheese was assayed for solids, acid degree value, and fat. Whey was assayed for total nitrogen, fat, and acid degree value. Milk and cheese weights were to the nearest. 1 g. Wet cheese yield increased by an average of 1.9% for cheese containing lecithin. Adjusted whey fats decreased and cheese fat increased slightly (not significant) in lecithin-treated milk and whey surface fat appeared to decrease. No treatment effect was observed for whey total nitrogen or acid degree values of cheese. Whey acid degree values were greater for the STA-SOL UF^TM, suggesting that the carrier oil on the whey surface contained some free fatty acids. Apparently, the increased yield was largely due to increased moisture content with a small increase from the milk fat. The resulting increase in fat may be an economic advantage to cheese manufacturers.     

Use of a β-glucan hydrocolloidal suspension in the manufacture of low-fat Cheddar cheese: manufacture, composition, yield and microstructure

Low‐fat Cheddar cheese was manufactured using a β‐glucan, hydrocolloidal fat replacer denoted as Nutrim. The composition, production efficiency, microstructure, and utility of replacing fat with Nutrim were examined. Cheese samples (designated as Nutrim‐I, and Nutrim‐II) containing Nutrim were produced with mean fat levels of 6.84 and 3.47%, respectively. A low‐fat cheese was also produced as a control with a mean fat level of 11.2%. Nutrim‐II cheese had significantly higher moisture, salt, and ash contents as compared with the low‐fat control cheese. The low‐fat control cheese had a higher yield normalized for 54% moisture and 1.5% salt content as compared with the Nutrim‐II cheese. Scanning electron microscopy revealed smaller and more uniform fat droplet voids in the Nutrim cheese than the low‐fat control, and a more dense, noncontinuous background protein matrix with globular clusters suggesting a physical buffering afforded by the presence of the β‐glucan hydrocolloid or its associated water.     

Replacement of fat with functional fibre in imitation cheese

The physical properties of imitation cheeses containing 21.3% dry matter (DM) resistant starch with moisture levels of 52–60%, as well as cheeses (60% moisture) containing 21.3–43.2% DM resistant starch were examined. Flowability of the heated cheese was assessed by an empirical method; the textural properties by texture profile analysis and dynamic rheology. Sensory analysis of cheeses was conducted by panellists using ranking tests. Increasing the moisture decreased cheese hardness did not affect flow but led to an undesirable ‘sticky’ and ‘clingy’ mouthfeel. Hardness increased linearly with increasing starch. Flow decreased at starch contents ⩾34.8% DM. Replacement of fat in the higher moisture cheeses resulted in the production of a good, firm cheese as assessed by a sensory panel. It was possible to incorporate up to 43.2% DM resistant starch into cheeses, a level higher than many ‘high fibre’ products, while maintaining acceptable functional properties.     

Effect of standardization of ewes'' milk for casein/fat ratio on the composition, sensory and rheological properties of Feta cheese

Ewes' milk standardized to four different casein/fat (C/F) ratios (0·80, 0·72, 0·67, 0·62) was used for Feta cheese manufacture. Cheese made from the low C/F ratio (0·62) milk had higher fat, fat in dry matter (FDM) and lower moisture and protein content than cheese made from the high C/F ratio (0·80) milk. With increase in C/F ratio, a significant decrease in fat, and FDM content and increase in protein content of Feta cheese was observed. The other components of cheese were not significantly affected by the C/F ratio of milk used. Also, the yield of cheese, expressed as kg of cheese/100 kg milk, decreased with increase in the C/F ratio of milk. On the other hand, yield expressed as kg of cheese with 56% moisture/kg milk fat increased with increasing C/F ratio. The sensory and rheological properties of cheese were not affected by the four C/F ratios of milk. Conclusions are drawn on the application of these results to Feta cheese manufacture.     

酶反应pH值对比萨干酪理化和功能特性的影响

研究了酶反应条件pH值对比萨干酪理化性质及功能特性的影响。选择在pH值为6.6与6.4时添加凝乳酶。通过对比萨干酪水分、脂肪及蛋白质质量分数的统计分析表明,酶反应条件pH值对比萨干酪的脂肪和蛋白质质量分数没有显著影响,对水分质量分数有显著影响。酶反应条件pH值为6.6比萨干酪水分质量分数为47.70%,pH值为6.4的水分质量分数为51.80%。通过测定干酪中pH值为4.6缓冲溶液及12%的TCA中可溶性氮质量分数,监测其水解情况。在贮藏过程中,可溶性氮质量分数不断增加。酶反应条件pH值影响比萨干酪的功能特性;酶反应条件pH值为6.6比萨干酪的硬度由最初为100.8N,50d后降低为88.9N,融化性增加了1.28cm,油析性增加了2.7%;pH值为6.4干酪功能特性的变化情况分别为:硬度降低为75.5N,融化性增加了2.16cm,油析性增加了3.1%。     

Composition, yield, and functionality of reduced-fat Oaxaca cheese: effects of using skim milk or a dry milk protein concentrate

The effect of adding either skim milk or a commercial dry milk protein concentrate (MPC) to whole milk on the composition, yield, and functional properties of Mexican Oaxaca cheese were investigated. Five batches of Oaxaca cheeses were produced. One batch (the control) was produced from whole milk containing 3.5% fat and 9% nonfat solids (SNF). Two batches were produced from milk standardized with skim milk to 2.7 and 1.8% fat, maintaining the SNF content at 9%. In the other 2 batches, an MPC (40% protein content) was used to standardize the milk to a SNF content of 10 and 11%, maintaining the milk fat content at 3.5%. The use of either skim milk or MPC caused a significant decrease in the fat percentage in cheese. The use of skim milk or MPC showed a nonsignificant tendency to lower total solids and fat recoveries in cheese. Actual, dry matter, and moisture-adjusted cheese yields significantly decreased with skim milk addition, but increased with MPC addition. However, normalized yields adjusted to milk fat and protein reference levels did not show significant differences between treatments. Considering skim milk-added and control cheeses, actual yield increased with cheese milk fat content at a rate of 1.34 kg/kg of fat (R = 0.88). In addition, cheese milk fat and SNF:fat ratio proved to be strong individual predictors of cheese moisture-adjusted yield (r² ≈ 0.90). Taking into account the results obtained from control and MPC-added cheeses, a 2.0-kg cheese yield increase rate per kg of milk MPC protein was observed (R = 0.89), with TS and SNF being the strongest predictors for moisture adjusted yield (r² ≈ 0.77). Reduced-fat Oaxaca cheese functionality differed from that of controls. In unmelted reduced-fat cheeses, hardness and springiness increased. In melted reduced-fat cheeses, meltability and free oil increased, but stretchability decreased. These changes were related to differences in cheese composition, mainly fat in dry matter and calcium in SNF.     

The effect of adding whey protein particles as inert filler on thermophysical properties of fat‐reduced semihard cheese type Gouda

The effect of added microparticulated whey protein (Simplesse^®) on textural and thermophysical properties of fat‐reduced semihard cheese type Gouda was investigated. Full‐fat, reduced‐fat and low‐fat cheeses were manufactured of comparable moisture content, each made of control milk and milk systems containing 1% Simplesse^®, respectively. Whey protein particles improved textural properties of reduced‐fat and low‐fat cheese. Meltability and flowability were significantly enhanced by an increased fat level, Simplesse^® addition and ripening time. The results emphasise the role of microparticulated whey proteins acting as an inert filler within the composite cheese matrix and allow textural and thermophysical properties of fat‐reduced cheeses to be adjusted towards cheese with higher fat content.     

Improving the quality of meat‐free sausages using κ‐carrageenan,konjac mannan and xanthan gum

High consumption of processed red meat can cause health issues. Therefore, production of high‐quality meat‐free food alternatives is necessary. The main objective of this study was to use hydrocolloids including κ‐carrageenan, konjac mannan and xanthan gum at 0, 0.3, 0.6, 1.0 and 1.5% w/w to improve the quality of meat‐free sausages formulated by soy protein isolated, texturised soy protein, corn starch, vegetable oil and spices. With the addition of the tested hydrocolloids, the lightness of the sausages improved. κ‐Carrageenan and konjac mannan significantly improved the water‐holding capacity and texture and reduced the cooking loss of the samples, while xanthan gum showed no considerable effect on these parameters. Konjac mannan and κ‐carrageenan (up to 0.6%) improved the general acceptability of the sausages, while inclusion of xanthan gum made no significant difference. Unlike xanthan gum, κ‐carrageenan followed by konjac mannan was highly successful in improving the overall quality of meat‐free sausages.     

The effects of carrageenan and pectin on some quality characteristics of low-fat beef frankfurters

Effects of carrageenan (0.3, 0.5, or 0.7%) and carrageenan (0.3, 0.5, or 0.7%) with a pectin gel (20%) on some quality characteristics of low-fat beef frankfurters were evaluated in comparison to a high-fat control (HFC) and a low-fat control (LFC). While low-fat frankfurters had <3.0% fat, 73-76% moisture, 13-14% protein, HFC had 17% fat, 59% moisture, and 14% protein. A reduction of 50-59% in cholesterol was determined in low fat beef frankfurters as compared to HFC (P<0.05). Better process yield and emulsion stability, and less purge were observed with increasing carrageenan concentration. Treatment groups showed higher water holding capacity (WHC) than LFC, and lower WHC than HFC (P<0.05). With increasing carrageenan concentration, WHC increased and penetrometer value decreased in low-fat frankfurters.