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微流控芯片发展与展望 总被引:4,自引:0,他引:4
介绍了微流控分析系统的一般特点、发展历史和近期的研究进展.分别讨论了微流控分析系统中有关流控系统、芯片材料、检测系统、集成化系统、分离系统、试样引入和前处理系统等研究领域的发展趋势,并对微流控分析系统的应用前景做出了展望. 相似文献
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SARS病毒的微流控芯片实验室系统检测 总被引:10,自引:0,他引:10
采用自行设计的RT-PCR试剂盒。在自制的由聚合酶链反应(PCR)——毛管电泳(CE)芯片、芯片热循环仪和激光诱导荧光芯片分析仪组成的微流控芯片系统上,对SARS病毒(重症急性呼吸综合症)和18例SARS患者咽拭子样品连行了分析和检测,实现了聚合酶链反应和电泳检测的微流控芯片在线分析。微流控芯片系统具有高检潮灵敏度、高分辨率、高检出率等优点,试剂消耗少和检测时间短,可能成为SARS早期检测的一种新的手段。 相似文献
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为解决在低聚焦流速下实现微流控芯片的高效聚焦和快速筛选功能及微流驱动控制系统小型化、低成本等技术问题,设计了一种微流控芯片及其微流驱动控制系统。该微流控芯片由控制层、PDMS薄膜层、流体通道层和玻璃基底等4层组成,流体通道层设置有1个主通道和3个分选通道,微流驱动控制系统中的液流控制装置采用单电动机时序驱动3个柱塞泵工作,控制层的热膨胀微阀门控制3个分选通道的开关。该微流控芯片及驱动系统实现了不同检测物的自动检测、聚焦、前进、静止、归类和筛选等功能。 相似文献
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提出了微流控芯片热压成型方法。介绍了热压成型的原理,并对微流控芯片热压成型机进行了总体设计。分析了热压成型机的液压系统及其器件选择方法,设计了热压成型机的整体液压回路,对液压系统进行了压力精度控制试验。试验结果表明,该系统控制精度在容许的范围之内。 相似文献
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针对微流控电泳芯片检测系统微型化、集成化的要求,分析了传统电泳芯片检测系统的优势和不足,提出一种以FPGA芯片为控制器的CCD多通道微流控电泳芯片检测系统。利用FPGA/NiosⅡ嵌入式系统解决方案,以EP2C8Q208芯片为核心,设计了CCD驱动及外围硬件电路。通过上位机软件进行数据处理,实现了荧光图谱的同步显示。实验结果表明:该系统能同时检测多通道微流控电泳芯片中各通道不同的荧光信号强度,具有较高的灵敏度和信噪比,对罗丹明B样品的最低检测浓度为1.0×10-6mol/L,能够满足多通道微流控电泳芯片检测的要求。 相似文献
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超声电机是一个复杂的机电耦合系统,其理论模型是电机设计中的重点和难点。本文对近20年来旋转型行波电机的理论建模进行了较全面总结,提出现今面临的新课题。提出经过子系统分析在状态空间中建立整个系统理论模型的思路,该模型将使超声电机的性能预测更准确更方便,为超声电机的优化设计打下基础。 相似文献
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State estimation is a widely adopted soft sensing technique that incorporates predictions from an accurate model of the process and measurements to provide reliable estimates of unmeasured variables. The reliability of such estimators is threatened by measurement related challenges and model inaccuracies. In this article, a method for benchmarking of state estimation techniques is proposed. This method can be used to quantify the performance and hence reliability of an estimator. The Hurst exponents of a posteriori filtering errors are analyzed to characterize a benchmark (minimum mean squared error) estimator, similar to the minimum variance control benchmark developed for control loops. A distance metric is then used to quantify the extent of deviation of an estimator from the benchmark. The proposed technique is developed for linear systems and extended to non-linear systems with single as well as multiple measurable variables. Simulation studies are carried out with Kalman based as well as Monte Carlo based estimators whose computational details are significantly different. Results reveal that the technique serves as a tool that can quantify the performance and assess the reliability of a state estimator. The strengths and limitations of the proposed technique are discussed with guidelines on applications and deployment of the technique in a real life system. 相似文献
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The current state of the art of the experimental approach to fretting is discussed. The mechanical response of fretting equipment is analysed to establish the limits of the reliable use of fretting rigs. The effect of stiffness and dynamic response of fretting test rigs are extensively discussed, and a simple one freedom-degree model is proposed to describe, in a first approach, the response of the tangential force measuring systems. 相似文献
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本文首先简要回顾了浑沌的发展历史,然后讨论了有浑沌的有关定义,探讨了浑沌的特征及有关分析方法,研究了浑沌系统的不变测度,等价变换以及控制问题。 相似文献
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Standard specification for power network quality diagnostic systems requires synchronization of sampling rate to the network frequency in every 10 s in order to ensure high accuracy of harmonic analysis. The total harmonic distortion (THD) cumulates measurement errors, therefore it is the most sensitive parameter to the frequency synchronization. This paper investigates two main effects putting impact on the THD computation accuracy. The first is the effect of the base clock rate of sampling hardware and the second is the practical deviation of network frequency during the 10 s synchronization period. The relative accuracy of THD calculation is examined in different real practical solutions and different synchronization methods are discussed and assessed. Theoretical proof and practical demonstration are given to show the needs of special synchronization methods in certain measuring circumstances. 相似文献
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首先介绍了面向对象的软件分析建模的重要性;并在分析比较目前流行的几种面向对象分析建模策略的基础上,着重介绍了在开发系统系列工具中采用的一种基于面向对象技术的分析建模策略;对分析建模过程中涉及的一些关键问题进行了探讨。 相似文献
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随动系统智能故障诊断与仿真综述 总被引:1,自引:0,他引:1
文章首先对随动系统的结构,性能及类型进行了介绍;然后重点对基于人工智能的随动系统故障诊断方法及相应软硬件检测平台的搭建分别进行介组,并给出了大量实例;最后针对随动系统试验条件有限的情况归纳了几种随动系统故障仿真方法。 相似文献