共查询到10条相似文献,搜索用时 46 毫秒
1.
2.
采用表面活性剂辅助法分别制备了铈、镧及其复合物的硫酸氧盐和掺加铜元素的硫酸氧铈,利用X射线衍射(XRD)、傅里叶红外光谱变换法(FT-IR)、BET比表面积测试法(BET)、程序升温还原法(TPR)、程序升温氧化(TPO)和氧脉冲吸附等技术手段对这些储氧材料进行了表征.结果表明,Ce2O2SO4、LaCeO2SO4的氧... 相似文献
3.
4.
研究通过浸渍—炭化法制备二维C/C复合材料层叠板的工艺参数,分析了不同基体前驱体和增密次数对材料的密度、厚度和收缩率、体积电阻率和层间剪切强度的影响,并用扫描电子显微镜进行断口分析。结果表明:选用残炭率较高的基体前驱体和适当的增密次数是制备低成本二维C/C复合材料层叠板的关键;相同纤维体积的层叠板基体炭含量越高,电阻率越小,导电性能越好;单位体积含有炭纤维越多,纤维受损几率就越大,产生结构缺陷几率越高,导致电阻率增加,导电性能下降;本实验中二维C/C复合材料层叠板制备工艺简单可行,层叠板的密度达到1.40g/cm^3以上,剪切强度为1.5MPa,断口呈脆性断裂特征。 相似文献
5.
6.
7.
Gleb Simanov Irene Dang Artem I. Fokin Ksenia Oguievetskaia Valrie Campanacci Jacqueline Cherfils Alexis M. Gautreau 《International journal of molecular sciences》2021,22(8)
During cell migration, protrusion of the leading edge is driven by the polymerization of Arp2/3-dependent branched actin networks. Migration persistence is negatively regulated by the Arp2/3 inhibitory protein Arpin. To better understand Arpin regulation in the cell, we looked for its interacting partners and identified both Tankyrase 1 and 2 (TNKS) using a yeast two-hybrid screening and coimmunoprecipitation with full-length Arpin as bait. Arpin interacts with ankyrin repeats of TNKS through a C-terminal-binding site on its acidic tail, which overlaps with the Arp2/3-binding site. Arpin was found to dissolve the liquid–liquid phase separation of TNKS upon overexpression. To uncouple the interactions of Arpin with TNKS and Arp2/3, we introduced point mutations in the Arpin tail and attempted to rescue the increased migration persistence of the Arpin knockout cells using random plasmid integration or compensating knock-ins at the ARPIN locus. Arpin mutations impairing interactions with either Arp2/3 or TNKS were insufficient to fully abolish Arpin activity. Only the mutation that affected both interactions rendered Arpin completely inactive, suggesting the existence of two independent pathways, whereby Arpin controls the migration persistence. 相似文献
8.
Rushuang Xu Shan He Di Ma Rui Liang Qing Luo Guanbin Song 《International journal of molecular sciences》2023,24(1)
Plectin, as a cytoskeleton-related protein, is involved in various physiological and pathological processes of many cell types. Studies have found that plectin affects cancer cell invasion and metastasis, but the exact mechanism is not fully understood. In this study, we aim to investigate the role of plectin in the migration of hepatocellular carcinoma (HCC) cells and explore its relevant molecular mechanism. Herein, we found that the expression of plectin in HCC tissue and cells was significantly increased compared with normal liver tissue and cells. After downregulation of plectin, the migration ability of HCC cells was significantly lower than that of the control group. Moreover, the expression of E-cadherin was upregulated and the expression of N-cadherin and vimentin was downregulated, suggesting that plectin downregulation suppresses epithelial mesenchymal transformation (EMT) of HCC cells. Mechanically, we found that plectin downregulation repressed the extracellular signal-regulated kinase 1/2 (ERK1/2) phosphorylation. Activation of ERK1/2 recovered the plectin downregulation-inhibited migration and EMT of HCC cells. Taken together, our results demonstrate that downregulation of plectin inhibits HCC cell migration and EMT through ERK1/2 signaling, which provides a novel prognostic biomarker and potential therapeutic target for HCC. 相似文献
9.
Tristen V. Tellman Lissette A. Cruz Brian J. Grindel Mary C. Farach-Carson 《International journal of molecular sciences》2021,22(6)
The Perlecan-Semaphorin 3A-Plexin A1-Neuropilin-1 (PSPN) Complex at the cell surface of prostate cancer (PCa) cells influences cell–cell cohesion and dyscohesion. We investigated matrix metalloproteinase-7/matrilysin (MMP-7)’s ability to digest components of the PSPN Complex in bone metastatic PCa cells using in silico analyses and in vitro experiments. Results demonstrated that in addition to the heparan sulfate proteoglycan, perlecan, all components of the PSPN Complex were degraded by MMP-7. To investigate the functional consequences of PSPN Complex cleavage, we developed a preformed microtumor model to examine initiation of cell dispersion after MMP-7 digestion. We found that while perlecan fully decorated with glycosaminoglycan limited dispersion of PCa microtumors, MMP-7 initiated rapid dyscohesion and migration even with perlecan present. Additionally, we found that a bioactive peptide (PLN4) found in perlecan domain IV in a region subject to digestion by MMP-7 further enhanced cell dispersion along with MMP-7. We found that digestion of the PSPN Complex with MMP-7 destabilized cell–cell junctions in microtumors evidenced by loss of co-registration of E-cadherin and F-actin. We conclude that MMP-7 plays a key functional role in PCa cell transition from a cohesive, indolent phenotype to a dyscohesive, migratory phenotype favoring production of circulating tumor cells and metastasis to bone. 相似文献
10.
Yu-Jen Wu Choo-Aun Neoh Chia-Yu Tsao Jui-Hsin Su Hsing-Hui Li 《International journal of molecular sciences》2015,16(7):16469-16482
Sinulariolide is an active compound isolated from the cultured soft coral Sinularia flexibilis. In this study, we investigate the migration and invasion effects of sinulariolide in hepatocellular carcinoma cell HA22T. Sinulariolide inhibited the migration and invasion effects of hepatocellular carcinoma cells in a concentration-dependent manner. The results of zymography assay showed that sinulariolide suppressed the activities of matrix metalloproteinase (MMP)-2 and MMP-9. Moreover, protein levels of MMP-2, MMP-9, and urokinase-type plasminogen activator (uPA) were reduced by sinulariolide in a concentration-dependent manner. Sinulariolide also exerted an inhibitory effect on phosphorylation of c-Jun N-terminal kinase (JNK), extracellular signal-regulated kinases (ERK), phosphatidylinositol 3-kinase (PI3K), Akt, Focal adhesion kinase (FAK), growth factor receptor-bound protein 2 (GRB2). Taken together, these results demonstrated that sinulariolide could inhibit hepatocellular carcinoma cell migration and invasion and alter HA22T cell metastasis by reduction of MMP-2, MMP-9, and uPA expression through the suppression of MAPKs, PI3K/Akt, and the FAK/GRB2 signaling pathway. These findings suggest that sinulariolide merits further evaluation as a chemotherapeutic agent for human hepatocellular carcinoma. 相似文献