共查询到19条相似文献,搜索用时 109 毫秒
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建立测定三七养血胶囊中人参皂苷Rg1、Re、Rb1、Rd及三七皂苷R1的含量测定方法。方法为:色谱柱为InertsilC8-3柱(5μm,4.6×250mm),流动相为乙腈-水,梯度洗脱,检测波长为203nm。结果表明:人参皂苷Rg1、Re、Rb1、Rd及三七皂苷R1的进样量分别在1.74~69.61μg(r=0.9997)、0.23~9.59μg(r=0.9998)、1.47~59.17μg(r=0.9998)、0.20~8.05μg(r=0.9997)、0.45~18.35μg(r=0.9999)范围内与峰面积积分值呈良好的线性关系;平均回收率(n=6)分别为0.57%、0.78%、1.08%、0.43%、1.24%。该方法灵敏准确、重复性好,专属性强,可用于本制剂的质量控制和评价。 相似文献
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高效液相色谱法测定农田人参中9种人参皂苷单体含量 总被引:2,自引:0,他引:2
为评价农田人参质量,建立同时测定农田人参中9种人参皂苷单体含量的方法。采用反相高效液相色谱法(reversed phase-high performance liquid chromatography,RP-HPLC)对农田人参与伐林人参中9种人参皂苷单体含量进行比较分析,色谱条件:色谱柱(4.6mm×150mm,5μm),乙腈(A)-水(B)为流动相,梯度洗脱[0min(18%A)→24min(22%A)→26min(26%A)→30min(32%A)→50min(33.5%A)→55min(38%A)],流速为1.0mL/min,检测波长203 nm,柱温35℃。结果表明:农田人参含有与伐林人参相同种类的9种人参皂苷Rg 1、Re、Rf、Rg2、Rb1、Rc、Rb2、Rb3、Rd;6年生农田人参9种皂苷含量均高于6年生伐林人参,但除Rg1含量差异显著外(P<0.05),其他8种皂苷含量均不显著(P>0.05);4年生农田人参除Rg1、Rf显著高于4年生伐林人参(P<0.05)外,其他7种皂苷含量与4年生伐林人参差异均不显著(P>0.05)。农田人参中Rgl、Rb1的含量、Rgl和Re含量之和、Rgl和Re含量之和均超过中国药典、欧洲药典与美国药典的要求。 相似文献
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目的建立高效液相色谱法(high performance liquid chromatography,HPLC)测定人参中人参皂苷Rg_1、Re和Rb_1 3种人参皂苷的含量。方法采用月旭Boltimate~(TM)C_(18)新型核壳色谱柱对3种人参皂苷进行分离,以乙腈-1.0%磷酸水溶液为流动相进行梯度洗脱,采用HPLC进行检测。结果 3种人参皂苷在月旭Boltimate~(TM)C_(18)新型核壳色谱柱能够快速分离,且分离效果较好。3种人参皂苷的在0.4~4.0μg质量浓度范围内呈良好的线性关系,线性相关系数(R~2)分别为0.9998,0.9996和0.9997;Rg_1、Re和Rb_1检出限分别为0.003、0.001和0.002μg/kg;加标回收率为98.3%~101.3%,相对标准偏差RSD分别为0.8%、1.1%和1.5%(n=6)。结论本方法具有快速、柱压低及节省溶剂等优势,可用于人参提取物中人参皂苷的测定。 相似文献
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超高效液相色谱法检测6种人参皂苷含量 总被引:1,自引:0,他引:1
采用超声提取药食同源植物(人参、西洋参、三七)中原人参三醇皂苷(Rg1、Re、Rf)和原人参二醇皂苷(Rb1、Rc、Rd),建立了超高效液相色谱(UPLC)检测方法。以50%甲醇溶液为提取剂,料液比1∶80(g∶m L),超声时间30 min。采用乙腈和0.05%磷酸水为流动相,梯度洗脱,检测波长为203 nm。该方法在质量浓度5~1 000μg/m L范围内线性良好,6种皂苷的最低检出限在22.5~51.0 mg/kg之间,平均回收率98.1%~105.5%。该方法准确、灵敏度高、重现性好、省时快捷,适合日常、大批量样品的检测。用此方法测定市售人参、西洋参及三七样品,结果表明3类样品中原人参二醇类皂苷含量高于三醇类皂苷,三七样品中Rg1的含量比人参和西洋参高,西洋参中Re含量高于人参和三七样品,而Rf仅在人参样品中检测到。 相似文献
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目的 建立HPLC法同时测定腰息痛胶囊中的三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1的含量。方法 采用Omini MP C18(4.6 mm×200 mm,5μm)为色谱柱;流动相为乙腈-0.1%磷酸溶液,梯度洗脱;流速为0.7 ml/min;检测波长为203 nm;柱温为25℃。结果 三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1分别在40.402~1010.500,40.480~1012.000,41.480~1037.000μg范围内呈线性关系(r<0.99);平均加样回收率分别为97.8%,97.5%,98.7%(RSD<2.0%,n=6);精密度、重复性等试验均符合规定。结论 此法简便易行、结果科学,可为中西药复方制剂腰息痛胶囊中的3种皂苷类成分定量控制提供技术依据。 相似文献
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目的:建立心源素胶囊的定性定量方法。方法:采用薄层色谱法对西洋参和五味子进行定性鉴别,用HPLC-ELSD测定心源素中有效成分三七皂苷R1和人参皂苷Rg1、Re、Rb1的含量;色谱条件:Discovery C18色谱柱,乙腈(10%水)-0.1%冰醋酸水溶液,梯度洗脱,流速1.0mL/min,柱温30℃;漂移管温度105.6℃,载气流速3.0L/min。结果:在薄层色谱鉴别中能准确鉴定出心源素中的五味子和西洋参;三七皂苷R1和人参皂苷Rg1、Re、Rb1的线性范围分别为715~35.75μg/mL、1500~75μg/mL、1514~75.7μg/mL、2928~146.4μg/mL,平均回收率分别为88.53%(RSD=2.68%)、100.61%(RSD=1.95%)、100.01%(RSD=2.03%)、100.28%(RSD=2.40%)。结论:该方法可作为心源素胶囊的质量控制方法。 相似文献
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本文研究了抗抑郁活性三七叶总皂苷提取物中的20(S)-原人参二醇(PPD)型人参皂苷成分。采用对照品以及文献对比相结合的方法,初步分析该活性提取物的人参皂苷成分;采用高效液相色谱法,色谱条件为:甲醇-0.2%磷酸溶液(70:30)为流动相,检测波长203 nm,柱温为30℃,同时测定提取物中人参皂苷Rb_1、Rc、Rb_2、Rb_3,三七皂苷Fc这5个代表性的PPD型人参皂苷活性成分的含量。结果鉴别出Rb_1、Rc、Fc、Rb_2、Rb_3等15个成分,其中13个为PPD型人参皂苷。HPLC含量测定中,Rb_1、Rc、Fc、Rb_2、Rb_3分离良好,分离度1.5以上,平均加样回收率分别为98.92±2.67%(RSD=2.73%)、99.51±1.33%(RSD=1.34%)、100.09±1.45%(RSD=1.45%)、100.53±2.24(RSD=2.22%)、100.12±1.74%(RSD=1.74%)。本研究表明抗抑郁活性三七叶总皂苷主要是PPD型人参皂苷成分,其中Rb_1、Rc、Fc、Rb_2、Rb_3的总含量高达38.67±0.18%。 相似文献
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西洋参保健食品中7种人参皂苷的高效液相色谱法测定 总被引:1,自引:0,他引:1
《食品与药品》2015,(4)
目的建立同时测定西洋参保健食品中7种主要皂苷成分(人参皂苷Rg1、Rg2、Re、Rb1、Rb2、Rc和Rd)含量的高效液相色谱测定法(HPLC)。方法应用HPLC-梯度洗脱法,采用Kromasil C18(4.6 mm×250mm,5μm)色谱柱,以0.1%磷酸为流动相A,乙腈为流动相B进行梯度洗脱,流速1.0 m L/min,检测波长203 nm。结果人参皂苷Rg1、Re、Rb1、Rg2、Rc、Rb2和Rd的检测浓度线性范围分别为0.056~0.56,0.203~2.03,0.324~3.24,0.105~1.05,0.048~0.48,0.104~1.04和0.152~1.52 mg/m L,r值均大于0.999;平均加样回收率均大于98.7%,相对标准偏差(RSD)均小于1.9%(n=6)。结论此法能快速、准确地测定西洋参保健食品中7种主要皂苷的含量。 相似文献
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Y.M. Wang Y.Q. Ma S.C. Bi X.D. Ma R. Guan S.H. Wang M.Q. Lu F.S. Shi S.H. Hu 《Journal of dairy science》2019,102(3):2443-2452
Escherichia coli is a cause of subclinical and clinical mastitis in dairy cattle and goats, and sometimes causes severe clinical disease that may result in death of the animal. Previous investigation showed that ginsenoside Rg1 extracted from Panax ginseng C.A. Meyer (Araliaceae) has an anti-inflammatory effect on the sepsis induced by E. coli lipopolysaccharide via competitive binding to toll-like receptor 4. We hypothesized that intravenous injection of Rg1 had therapeutic effect on mastitis experimentally induced by intramammary infusion of lipopolysaccharide in lactating goats. In this study, 9 lactating goats were randomly assigned to 1 of the 3 groups: (1) lipopolysaccharide intramammary infusion + saline intravenous injection, (2) lipopolysaccharide intramammary infusion + Rg1 intravenous injection, and (3) saline intramammary administration + saline intravenous injection. Because no adverse clinical signs were observed after intramammary infusion of saline and intravenous injection of Rg1 in a preliminary experiment, and available qualified goats were limited in this study, this treatment was not included in this study. One udder half of each goat received intramammary infusion of lipopolysaccharide (50 μg/kg of body weight; groups 1 and 2) or saline solution (group 3), and the other half was infused with 2 mL of saline solution at h 0. Afterward, intravenous injections of saline solution (groups 1 and 3) or Rg1 (2.5 mg/kg of body weight; group 2) were administered at h 2 and 4 post-lipopolysaccharide challenge. Blood and milk samples were collected 3, 6, 9, 12, 15, 18, 21, 24, 48, and 72 h post-lipopolysaccharide challenge, and clinical signs were monitored hourly after lipopolysaccharide challenge within the first 10 h and at the same time points as blood samples. The results showed that Rg1 treatment downregulated rectal temperature, udder skin temperature, udder girth, milk somatic cell count, and N-acetyl-β-d-glucosaminidase and upregulated milk production, lactose, and recovered blood components, such as white blood cells, neutrophils, lymphocytes, total proteins, albumin, and globulin. Considering the positive therapeutic effect on lipopolysaccharide-induced mastitis in goats presented in this study as well as the anti-inflammatory activity found previously, the botanical Rg1 deserves further study as a therapeutic agent in the treatment of E. coli mastitis in dairy animals. 相似文献
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为了研究人参皂苷 Rg1 对疲劳模型小鼠抗疲劳作用及骨骼肌中线粒体转录因子 A(TFAM)和核呼吸因子-1(NRF-1)基因表达的影响,选取 70 只雄性 BALB/c 小鼠随机分为7组,除空白组外,小鼠腹腔注射环磷酰胺,造成免疫低下模型后进行小鼠负重游泳力竭实验。ELISA 法测定小鼠肝脏及骨骼肌中疲劳相关指标的变化情况,RT-PCR 法检测小鼠骨骼肌中 TFAM 和 NRF-1 基因表达水平。结果表明,人参皂苷 Rg1 中剂量组小鼠负重游泳的力竭时间最长,较空白组增加了 271.4%,且能显著增加肝糖原浓度。各剂量组小鼠肝脏 MDA 质量摩尔浓度下降,LDH 活性升高。中剂量人参皂苷 Rg1 可显著增加小鼠骨骼肌中 TFAM mRNA 和 NRF-1 mRNA 表达水平。人参皂苷 Rg1 可有效调节疲劳小鼠骨骼肌中疲劳相关生化指标的水平,具有较好的抗疲劳效果。 相似文献
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Red ginseng has a variety of bioactive functions and is widely used as an oriental medicinal herb and food ingredient. The aim of this study was to investigate the antiviral effect of red ginseng extract and ginsenosides against hepatitis A virus (HAV). To examine the antiviral effect against HAV, 0 to 10 μg/mL of red ginseng and purified ginsenoside Rb1 and Rg1 were pre‐treated or co‐treated on FRhK‐4 cells. The HAV titer decreased significantly in all groups pretreated with red ginseng or purified ginsenosides. The reduction of HAV was significant in FRhK‐4 cells pre‐treated only with red ginseng. Our results showed that red ginseng and ginsenoside Rg1 and Rb1 could decrease HAV titers. 相似文献
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目的建立高效液相色谱法同时检测抗疲劳类功能食品中红景天苷、淫羊藿苷、人参皂苷Rg1和葛根素4种有效成分的分析方法。方法样品中的待测组分经乙腈水溶液超声提取、过滤后,采用SHIFEIDO C_(18)柱(4.6mm×250mm,5μm)为分离柱,以乙腈(A)-水(B)为流动相,梯度洗脱流速为1.0mL/min;检测波长为203 nm;柱温为40℃,标准曲线法定量。结果 4种化合物分离度良好,线性相关系数均大于0.9996,回收率为96.4%~103.7%,检出限为5.2~50.0μg/g,定量限为15.5~151.6μg/g。结论本方法简便、准确,可用于抗疲劳类功能食品中功效成分的检测。 相似文献
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为探讨1-甲基环丙烯(1-methylcyclopropene,1-MCP)处理对‘粉红女士’苹果采后生理特性及其品质的影响。用1.0 μL/L的1-MCP处理‘粉红女士’苹果24 h,以不处理为对照,20℃条件下贮藏,定期测定贮藏期间的主要品质及相关生理指标。结果表明:1-MCP处理可保持苹果贮藏过程中的硬度、可滴定酸含量,延缓果皮油腻化的发生,显著抑制果实的呼吸强度和乙烯释放速率,推迟果实过氧化氢酶、过氧化物酶两种酶活性峰值的出现,并在一定程度上提高了超氧化物歧化酶和过氧化氢酶的活性,有效抑制丙二醛含量的积累。因此,1-MCP处理可较好地调控‘粉红女士’苹果的相关生理代谢,改善果实的贮藏品质,从而有效延缓果实采后的成熟衰老进程。 相似文献