贯叶金丝桃(贯叶连翘)研究进展

介绍了贯叶金丝桃的植物形态及分布,化学成分及分布规律,采集、干燥、加工、贮存及质量标准,药理及临床应用,国外管理现状等五方面的情况。     

贯叶连翘中金丝桃素的超声提取工艺研究

通过实验筛选贯叶连翘中金丝桃素的超声提取工艺,为生产提供依据。采用单因素实验结合正交实验方法研究超声提取条件,以金丝桃素的提取率为考察指标,分析了影响金丝桃素提取率的因素。结果表明,贯叶连翘中金丝桃素的最佳提取工艺为采用70%的乙醇为提取溶剂。15倍于药材量的溶剂,提取2次,每次40 min,提取率为92.42%。经过3批验证实验,筛选出的工艺稳定,适用于生产。     

高纯度金丝桃素生产工艺研究

利用大孔吸附树脂吸附法研究了金丝桃素的提取分离方法.结果表明,采用HZ 816大孔吸附树脂对贯叶连翘粗提物进行吸附纯化,以水、40%乙醇、60%乙醇洗去大量杂质后,以80%乙醇解吸,所得样品再经酸水结晶可得高纯度的金丝桃素.该方法简便,消耗低,产品纯度高,回收率高.     

HPLC法测定不同产地贯叶连翘中的金丝桃素含量

测定不同产地贯叶连翘中的金丝桃素含量.方法:采用HPLC方法测定金丝桃素含量.结果:不同产地贯叶连翘中的金丝桃素含量差异显著,5个样品中,其中陕西产的贯叶连翘中金丝桃素含量最高,湖南产贯叶连翘中的金丝桃素含量最低.     

贯叶连翘中金丝桃素提取分离方法研究

研究了从贯叶连翘中提取分离金丝桃素,探讨了提取剂、料液比、提取次数、提取温度、提取时间等因素对金丝桃素提取率的影响,并通过正交实验对提取工艺进行了优化。实验表明:提取最佳条件为将原料在45℃水浸泡2 h,以75%的甲醇为提取剂,超声强化20 m in(功率1000 W),按料液比1∶6和1∶4各提取1次,每次3 h,LSI-106型大孔树脂分离,以甲醇洗脱(洗脱速度1~2 mL/m in),提取率为96.36%,得率为5.33 mg/10 g,纯度达到94.71%,同时测得金丝桃素的稳定性(RSD=0.586%)良好。     

TLC-分光光度法测贯叶连翘中金丝桃素含量

:针对贯叶连翘中成分复杂 ,干扰物多的特点 ,采用薄层层析 (TL C)分离出金丝桃素后 ,再进行分光光度测定。薄层层析以石油醚 -乙酸乙酯 -甲醇 (1∶ 4∶ 3)为展开体系展开 ,金丝桃素的 Rf=0 .85。分光光度法测定的回归方程为 A=72 .0 0 C+0 .0 0 3(r=0 .9997) ,平均回收率为 97.9% (RSD=0 .87% )。     

贯叶连翘中金丝桃甙的提取工艺研究

探讨了贯叶连翘中金丝桃甙的提取工艺,研究表明,溶剂的极性,介质的酸碱性,提取液的含水量,提取物的浓缩,产品的干燥方式等工艺条件对其有效成分的纯度和提取率有很大的影响,所述结论为金丝桃甙的工业生产提供了依据。     

微波提取贯叶连翘中金丝桃素的工艺研究

通过微波提取方法研究贯叶连翘中金丝桃素的提取工艺。通过单因素实验研究了微波功率,液固比,乙醇浓度,微波温度以及微波时间对金丝桃素提取率的影响,通过二次回归通用旋转组合设计研究了微波功率,液固比,乙醇浓度,微波温度以及微波时间对提取率的影响。二次回归通用旋转组合设计的最优工艺条件是:17.5倍量的65%的乙醇溶液,在65℃下用700 W的微波提取64 min。通过微波提取方法较传统的浸提法具有耗时短,提取率高的特点,具有较好的应用前景。     

贯叶连翘中金丝桃甙的提取工艺研究

探讨了贯叶连翘中金丝桃甙的提取工艺。研究表明,溶剂的极性、介质的酸碱性、提取液的含水量、提取物的浓缩、产品的干燥方式等工艺条件对其有效成分的纯度和提取率有很大的影响。所述结论为金丝桃甙的工业生产提供了依据。     

超声波碱水法提取贯叶连翘总黄酮

用超声波碱水法提取贯叶连翘总黄酮,通过正交试验设计,对碱水浓度、提取时间、固液比进行优化,再对三种大孔吸附树脂进行静态吸附和洗脱效果的比较,选择适合的型号。结果是对得率影响最大的因素为碱水浓度,其次为固液比和提取时间;最佳提取工艺条件为30倍量的2%碱水,超声辅助提取60 min。在三种型号的大孔吸附树脂中AB-8的静态吸附及洗脱效果最好。     

乙醇法提取金丝桃素的研究

介绍了利用乙醇对贯叶连翘中金丝桃素进行提取的工艺研究。探讨了提取金丝桃素时提取剂用量、提取温度、提取时间和提取剂浓度对贯叶连翘提取物的得率和其中金丝桃素含量的影响。优选出最佳工艺条件,为工业化生产提供了理论依据。     

大孔树脂与黄酮类化合物的固液界面吸附性能的研究

通过比较D101-A、D138、DM-130这3种大孔树脂和聚酰胺树脂对苦荞粉提取液中黄酮类化合物的静态、动态吸附及解吸性能,并研究其相应的静态吸附动力学过程和黄酮类化合物的浓度对吸附性能的影响,以选择最佳的吸附树脂。试验结果表明:D-101A、D138、DM-130大孔树脂和聚酰胺树脂这4种树脂对黄酮类化合物的吸附能力非常接近,都可用来作为黄酮类化合物的吸附剂。     

Response Surface Optimized Ultrasonic-Assisted Extraction of Quercetin and Isolation of Phenolic Compounds From Hypericum perforatum L. by Column Chromatography

The effects of ultrasonic-assisted extraction factors for the main phenolic compound (quercetin) from Hypericum perforatum L. were optimized using the Box–Behnken design (BBD) combined with response surface methodology. The BBD was employed to evaluate the effects of extraction temperature (30–70°C), extraction time (20–80 min), methanol concentration (20–80%, v/v), and HCl concentration (0.8–2.0 M) on the content of one of the major phenolic compounds of quercetin. The extracts were analyzed by high performance liquid chromatography (HPLC). The major phenolic compounds of H. perforatum were isolated and the antioxidant capacity and total phenol content were determined in crude extract and fractions. The optimum conditions were determined as extraction temperature 67°C, extraction time 67 min, methanol concentration 77% (v/v), and HCl concentration 1.2 M. The predicted content of quercetin was 10.81 mg/g dried plant under the optimal conditions and the subsequent verification experiment with 11.09 mg/g dried plant confirmed the validity of the predicted model. The isolated compounds were identified as quercetin, cyanidin, protocatechuic acid, and kaempferol.     

An in vitro evaluation of Genipin-crosslinked and Hypericum perforatum incorporated novel membranes for skin tissue engineering applications

Incorporating medicinal plant extracts in membranes have a great potential as scaffolds for tissue engineering applications or vehicles for delivering therapeutic agents. Herein, Hypericum perforatum oil (0.25, 0.50, % vol/vol) loaded membranes were developed with Polyvinyl alcohol and chitosan polymer, where Genipin works as a chemical crosslinker to obtain a wound dressing material with acceptable characterization properties. Chemical groups, surface morphology, water uptake capacity, water vapor permeability rate, hydrophilicity, and mechanical properties of membranes were thoroughly investigated. Increasing oil concentration had a significant effect on the water uptake, surface morphology. and water vapor permeability rate of the membranes. Cytocompatibility of the membrane was also investigated with mouse embryonic fibroblasts (MEF) by 3-(4,5-dimethylthiazoyl-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay for direct and indirect cell culture studies. SEM was used to investigate the cell morphology on the membranes. The MTT assay findings prove that Genipin crosslinked H. perforatum oil loaded scaffolds are highly biocompatible and enhance the adhesion and proliferation of MEF cells. In addition to this, the genotoxicity test was performed to show DNA fragmentation. Results showed that the H. perforatum oil loaded polyvinyl alcohol-chitosan membrane presents suitable properties for potential skin tissue engineering applications.     

大孔树脂分离纯化沙芥黄酮的工艺研究

用大孔吸附树脂分离纯化沙芥总黄酮,比较了7种大孔树脂对沙芥总黄酮的静态吸附动力学特性,优选出D-4020型大孔吸附树脂分离纯化沙芥总黄酮,并对其进行动态吸附实验。结果表明,D-4020纯化沙芥总黄酮的最佳工艺参数为:上样液浓度0.4 mg/mL,pH值5,上样流速2 mL/min;使用4BV用量95%的乙醇作为洗脱剂,洗脱流速为2 mL/min。采用该工艺分离纯化沙芥总黄酮含量达40.91%。     

Preparative enrichment and purification of nevadensin from Lysionotus pauciflorus using macroporous resins

An efficient method using macroporous adsorption resins for preparative enrichment and purification of nevadensin from Lysionotus pauciflorus Maxim was developed. The results indicated that non-polar HPD-100 resin offered the best adsorption and desorption performance, its adsorption data were well-fitted to the Freundlich isotherm and the pseudo-second-order kinetics model. Dynamic adsorption and desorption experiments have been investigated for optimization of chromatographic parameters. Through one cycle of dynamic adsorption/desorption, the purity of nevadensin in the extract, increased about 8.82-fold from 8.58% to 75.70%, with a recovery yield of 69.90%. The results suggested that HPD-100 resin can separate nevadensin effectively from plant material.