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1.
C‐Alkyl amidine analogues of asymmetric Nω,Nω‐dimethyl‐L ‐arginine are dual‐targeted inhibitors of both human DDAH‐1 and nitric oxide (NO) synthase, and provide a promising scaffold for the development of therapeutics to control NO overproduction in a variety of pathologies including septic shock and some cancers. Using a two‐part click‐chemistry‐mediated activity probe, a homologated series of C‐alkyl amidines were ranked for their ability to inhibit DDAH‐1 within cultured HEK 293T cells. N5‐(1‐Iminopentyl)‐L ‐ornithine was determined to be the most potent compound in vitro (Kd=7 μM ) as well as in cultured cells, and the binding conformation and covalent reversible mode of inhibition was investigated by comparison of interactions made with DDAH‐1 and a catalytically inactive C274S variant, as gauged by X‐ray crystallography and isothermal titration calorimetry. By interrupting the ability of the inhibitor to form a covalent bond, the contribution of this interaction could be estimated. These results suggest that further stabilization of the covalent adduct is a promising strategy for lead optimization in the design of effective reagents to block NO synthesis. 相似文献
2.
New Insights into the Anti‐Inflammatory and Antioxidant Properties of Nitrated Phospholipids
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Tânia Melo Sara S. Marques Isabel Ferreira Maria Teresa Cruz Pedro Domingues Marcela A. Segundo Maria Rosário Marques Domingues 《Lipids》2018,53(1):117-131
Nitro‐fatty acids (NO2‐FA) have been widely studied with regard to their identification, structural characterization, and biological actions. NO2‐FA could also be present endogenously esterified to phospholipids (PL), and NO2‐PL were already detected in cardiac mitochondria from diabetic rats and cardiomyoblasts subjected to starvation. However, the biological actions of NO2‐PL have been overlooked. In this study, we evaluate the antioxidant and anti‐inflammatory potential of the nitrated 1‐palmitoyl‐2‐oleoyl‐sn‐glycero‐3‐phosphocholine (POPC) formed in vitro by incubation with NO2BF4, in a well‐recognized mimetic model of nitroxidative stress. Nitrated POPC showed anti‐radical ability to reduce both 2,2‐diphenyl‐1‐picrylhydrazyl radical (DPPH?) (IC20 = 225 ± 4 μg/mL; Trolox equivalent (TE) = 86 ± 6 μmol Trolox/g lipid) and 2,2′‐azino‐bis‐3‐ethylbenzothiazoline‐6‐sulfonic acid radical cation (ABTS?+) (IC50 = 124 ± 2 μg/mL; TE = 152 ± 9 μmol Trolox/g lipid). Also, higher lag times were achieved in oxygen radical absorbance capacity (ORAC) assay for nitrated POPC, indicating a faster reaction with oxygen‐derived radicals (TE = 1.03 ± 0.22 and TE = 1.30 ± 0.16 mmol Trolox/g lipid for nonmodified and nitrated POPC, respectively). Nitrated POPC showed the ability to inhibit lipid oxidation induced by the hydroxyl radical generated under Fenton reaction conditions, monitored by electrospray ionization (ESI) mass spectrometry (MS) using phosphatidylcholine (PtdCho) liposomes as a model of cell membrane. Nitrated POPC showed anti‐inflammatory potential, as assessed by the inhibition of inducible nitric oxide synthase (iNOS) expression in RAW 264.7 macrophages activated by the Toll‐like receptor 4 (TLR4) agonist lipopolysaccharide (LPS) in a well‐described in vitro model of inflammation. Altogether, this study provides new clues regarding the antioxidant and anti‐inflammatory potential of nitrated POPC, which should be explored in depth. 相似文献
3.
Dichlorophenylacrylonitriles as AhR Ligands That Display Selective Breast Cancer Cytotoxicity in vitro
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Jennifer R. Baker Dr. Jayne Gilbert Prof. Stefan Paula Dr. Xiao Zhu Dr. Jennette A. Sakoff Prof. Adam McCluskey 《ChemMedChem》2018,13(14):1447-1458
Knoevenagel condensation of 3,4‐dichloro‐ and 2,6‐dichlorophenylacetonitriles gave a library of dichlorophenylacrylonitriles. Our leads (Z)‐2‐(3,4‐dichlorophenyl)‐3‐(1H‐pyrrol‐2‐yl)acrylonitrile ( 5 ) and (Z)‐2‐(3,4‐dichlorophenyl)‐3‐(4‐nitrophenyl)acrylonitrile ( 6 ) displayed 0.56±0.03 and 0.127±0.04 μm growth inhibition (GI50) and 260‐fold selectivity for the MCF‐7 breast cancer cell line. A 2,6‐dichlorophenyl moiety saw a 10‐fold decrease in potency; additional nitrogen moieties (‐NO2) enhanced activity (Z)‐2‐(2,6‐dichloro‐3‐nitrophenyl)‐3‐(2‐nitrophenyl)acrylonitrile ( 26 ) and (Z)‐2‐(2,6‐dichloro‐3‐nitrophenyl)‐3‐(3‐nitrophenyl)acrylonitrile ( 27 ), with the corresponding ‐NH2 analogues (Z)‐2‐(3‐amino‐2,6‐dichlorophenyl)‐3‐(2‐aminophenyl)acrylonitrile ( 29 ) and (Z)‐2‐(3‐amino‐2,6‐dichlorophenyl)‐3‐(3‐aminophenyl)acrylonitrile ( 30 ) being more potent. Despite this, both 29 (2.8±0.03 μm ) and 30 (2.8±0.03 μm ) were found to be 10‐fold less cytotoxic than 6 . A bromine moiety effected a 3‐fold enhancement in solubility with (Z)‐3‐(5‐bromo‐1H‐pyrrol‐2‐yl)‐2‐(3,4‐dichlorophenyl)acrylonitrile 18 relative to 5 at 211 μg mL?1. Modeling‐guided synthesis saw the introduction of 4‐aminophenyl substituents (Z)‐3‐(4‐aminophenyl)‐2‐(3,4‐dichlorophenyl)acrylonitrile ( 35 ) and (Z)‐N‐(4‐(2‐cyano‐2‐(3,4‐dichlorophenyl)vinyl)phenyl)acetamide ( 38 ), with respective GI50 values of 0.030±0.014 and 0.034±0.01 μm . Other analogues such as 35 and 36 were found to have sub‐micromolar potency against our panel of cancer cell lines (HT29, colon; U87 and SJ‐G2, glioblastoma; A2780, ovarian; H460, lung; A431, skin; Du145, prostate; BE2‐C, neuroblastoma; MIA, pancreas; and SMA, murine glioblastoma), except compound 38 against the U87 cell line. A more extensive evaluation of 38 ((Z)‐N‐(4‐(2‐cyano‐2‐(3,4‐dichlorophenyl)vinyl)phenyl)acetamide) in a panel of drug‐resistant breast carcinoma cell lines showed 10–206 nm potency against MDAMB468, T47D, ZR‐75‐1, SKBR3, and BT474. Molecular Operating Environment docking scores showed a good correlation between predicted binding efficiencies and observed MCF‐7 cytotoxicity. This supports the use of this model in the development of breast‐cancer‐specific drugs. 相似文献
4.
In this article, the binding characteristics of the imprinted polymer P‐1[CoII(salen)] (salen: bis(2‐hydroxybenzaldehyde)ethylenediimine) to nitric oxide (NO) have been reported. P‐1[CoII(salen)] was characterized by Fourier transform infrared analysis, thermogravimetric analysis, and differential scanning calorimetry. Batch‐mode adsorption studies were carried out to investigate binding thermodynamics, kinetics, and selective recognition behavior of P‐1[CoII(salen)] to NO. The kinetics study indicates that binding of the polymer to NO fits the first‐order reaction kinetics with the rate constant k1 of 0.087 min?1. Langmuir and Freundlich equations were used to explain the equilibrium character of P‐1[CoII(salen)] binding to NO. The r2 and χ2 values suggest that total amount of NO bound by P‐1[CoII(salen)] can be best fitted by the Langmuir equation. The binding capacity (Bmax) of P‐1[CoII(salen)] was calculated to be 76.28 μmol/g, very close to the experimental value, 75 μmol/g. The thermodynamics and selectivity experiments showed that the affinity of P‐1[CoII(salen)] to NO was much higher than carbon dioxide (CO2) and oxygen (O2), suggesting that P‐1[CoII(salen)] is a promising functional material for NO storage and NO sensing. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013 相似文献
5.
Johannes Jacobi Renke Maas Michaela Arend Nada Cordasic Karl F. Hilgers 《International journal of molecular sciences》2014,15(4):5522-5535
The purpose of the work was to study the impact of the endogenous nitric oxide synthase (NOS) inhibitor asymmetric dimethylarginine (ADMA) and its degrading enzyme, dimethylarginine dimethylaminohydrolase (DDAH1), on atherosclerosis in subtotally nephrectomized (SNX) ApoE-deficient mice. Male DDAH1 transgenic mice (TG, n = 39) and C57Bl/6J wild-type littermates (WT, n = 27) with or without the deletion of the ApoE gene underwent SNX at the age of eight weeks. Animals were sacrificed at 12 months of age, and blood chemistry, as well as the extent of atherosclerosis within the entire aorta were analyzed. Sham treated (no renal mass reduction) ApoE-competent DDAH1 transgenic and wild-type littermates (n = 11) served as a control group. Overexpression of DDAH1 was associated with significantly lower ADMA levels in all treatment groups. Surprisingly, SNX mice did not exhibit higher ADMA levels compared to sham treated control mice. Furthermore, the degree of atherosclerosis in ApoE-deficient mice with SNX was similar in mice with or without overexpression of DDAH1. Overexpression of the ADMA degrading enzyme, DDAH1, did not ameliorate atherosclerosis in ApoE-deficient SNX mice. Furthermore, SNX in mice had no impact on ADMA levels, suggesting a minor role of this molecule in chronic kidney disease (CKD) in this mouse model. 相似文献
6.
Jelena M. Lazić Ljubica Vučićević Sanja Grgurić‐Šipka Dr. Kristina Janjetović Goran N. Kaluđerović Dr. Maja Misirkić Maja Gruden‐Pavlović Dr. Dušan Popadić Prof. Reinhard Paschke Dr. Vladimir Trajković Prof. Tibor J. Sabo Prof. 《ChemMedChem》2010,5(6):881-889
The present study describes the synthesis and anticancer activity of novel octahedral PtIV complexes with cyclohexyl functionalized ethylenediamine‐N,N′‐diacetate‐type ligands. Molecular mechanics calculations and density functional theory analysis revealed that s‐cis is the preferred geometry of these PtIV complexes with tetradentate‐coordinated (S,S)‐ethylenediamine‐N,N′‐di‐2‐(3‐cyclohexyl)propanoate. The viability of cancer cell lines (U251 human glioma, C6 rat glioma, L929 mouse fibrosarcoma, and B16 human melanoma) was assessed by measuring mitochondrial dehydrogenase activity and lactate dehydrogenase release. Cell‐cycle distribution, oxidative stress, caspase activation, and induction of autophagy were analyzed by flow cytometry using appropriate fluorescent reporter dyes. The cytotoxic activity of novel PtIV complexes against various cancer cell lines (IC50 range: 1.9–8.7 μM ) was higher than that of cisplatin (IC50 range: 10.9–67.0 μM ) and proceeded through completely different mechanisms. Cisplatin induced caspase‐dependent apoptosis associated with the cytoprotective autophagic response. In contrast, the new PtIV complexes caused rapid, caspase‐independent, oxidative stress‐mediated non‐apoptotic cell death characterized by massive cytoplasmic vacuolization, cell membrane damage, and the absence of protective autophagy. 相似文献
7.
Peter van der Maas Loes Harmsen Sander Weelink Bram Klapwijk Piet Lens 《Journal of chemical technology and biotechnology (Oxford, Oxfordshire : 1986)》2004,79(8):835-841
The biological reduction of nitric oxide (NO) in aqueous solutions of FeEDTA is an important key reaction within the BioDeNOx process, a combined physico‐chemical and biological technique for the removal of NOx from industrial flue gasses. To explore the reduction of nitrogen oxide analogues, this study investigated the full denitrification pathway in aqueous FeEDTA solutions, ie the reduction of NO3?, NO2?, NO via N2O to N2 in this unusual medium. This was done in batch experiments at 30 °C with 25 mmol dm?3 FeEDTA solutions (pH 7.2 ± 0.2). Also Ca2+ (2 and 10 mmol dm?3) and Mg2+ (2 mmol dm?3) were added in excess to prevent free, uncomplexed EDTA. Nitrate reduction in aqueous solutions of Fe(III)EDTA is accompanied by the biological reduction of Fe(III) to Fe(II), for which ethanol, methanol and also acetate are suitable electron donors. Fe(II)EDTA can serve as electron donor for the biological reduction of nitrate to nitrite, with the concomitant oxidation of Fe(II)EDTA to Fe(III)EDTA. Moreover, Fe(II)EDTA can also serve as electron donor for the chemical reduction of nitrite to NO, with the concomitant formation of the nitrosyl‐complex Fe(II)EDTA–NO. The reduction of NO in Fe(II)EDTA was found to be catalysed biologically and occurred about three times faster at 55 °C than NO reduction at 30 °C. This study showed that the nitrogen and iron cycles are strongly coupled and that FeEDTA has an electron‐mediating role during the subsequent reduction of nitrate, nitrite, nitric oxide and nitrous oxide to dinitrogen gas. Copyright © 2004 Society of Chemical Industry 相似文献
8.
Carla S. Jung Christian Wispel Klaus Zweckberger Christopher Beynon Daniel Hertle Oliver W. Sakowitz Andreas W. Unterberg 《International journal of molecular sciences》2014,15(3):4088-4103
Previous results on nitric oxide (NO) metabolism after traumatic brain injury (TBI) show variations in NO availability and controversial effects of exogenous nitric oxide synthase (NOS)-inhibitors. Furthermore, elevated levels of the endogenous NOS inhibitor asymmetric dimethylarginine (ADMA) were reported in cerebro-spinal fluid (CSF) after traumatic subarachnoid hemorrhage (SAH). Therefore, we examined whether ADMA and the enzymes involved in NO- and ADMA-metabolism are expressed in brain tissue after TBI and if time-dependent changes occur. TBI was induced by controlled cortical impact injury (CCII) and neurological performance was monitored. Expression of NOS, ADMA, dimethylarginine dimethylaminohydrolases (DDAH) and protein-arginine methyltransferase 1 (PRMT1) was determined by immunostaining in different brain regions and at various time-points after CCII. ADMA and PRMT1 expression decreased in all animals after TBI compared to the control group, while DDAH1 and DDAH2 expression increased in comparison to controls. Furthermore, perilesionally ADMA is positively correlated with neuroscore performance, while DDAH1 and DDAH2 are negatively correlated. ADMA and its metabolizing enzymes show significant temporal changes after TBI and may be new targets in TBI treatment. 相似文献
9.
Designing Dual Transglutaminase 2/Histone Deacetylase Inhibitors Effective at Halting Neuronal Death
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Dr. Manuela Basso Dr. Huan Huan Chen Dr. Debasmita Tripathy Dr. Mariarosaria Conte Dr. Kim Y. P. Apperley Dr. Angela De Simone Prof. Dr. Jeffrey W. Keillor Prof. Dr. Rajiv Ratan Dr. Angela Nebbioso Dr. Federica Sarno Prof. Dr. Lucia Altucci Dr. Andrea Milelli 《ChemMedChem》2018,13(3):227-230
In recent years there has been a clear consensus that neurodegenerative conditions can be better treated through concurrent modulation of different targets. Herein we report that combined inhibition of transglutaminase 2 (TG2) and histone deacetylases (HDACs) synergistically protects against toxic stimuli mediated by glutamate. Based on these findings, we designed and synthesized a series of novel dual TG2–HDAC binding agents. Compound 3 [(E)‐N‐hydroxy‐5‐(3‐(4‐(3‐oxo‐3‐(pyridin‐3‐yl)prop‐1‐en‐1‐yl)phenyl)thioureido)pentanamide] emerged as the most interesting of the series, being able to inhibit TG2 and HDACs both in vitro (TG2 IC50=13.3±1.5 μm , HDAC1 IC50=3.38±0.14 μm , HDAC6 IC50=4.10±0.13 μm ) and in cell‐based assays. Furthermore, compound 3 does not exert any toxic effects in cortical neurons up to 50 μm and protects neurons against toxic insults induced by glutamate (5 mm ) with an EC50 value of 3.7±0.5 μm . 相似文献
10.
Luis Aldámiz-Echevarría Fernando Andrade 《International journal of molecular sciences》2012,13(9):11288-11311
l-Arginine (Arg) is oxidized to l-citrulline and nitric oxide (NO) by the action of endothelial nitric oxide synthase (NOS). In contrast, protein-incorporated Arg residues can be methylated with subsequent proteolysis giving rise to methylarginine compounds, such as asymmetric dimethylarginine (ADMA) that competes with Arg for binding to NOS. Most ADMA is degraded by dimethylarginine dimethyaminohydrolase (DDAH), distributed widely throughout the body and regulates ADMA levels and, therefore, NO synthesis. In recent years, several studies have suggested that increased ADMA levels are a marker of atherosclerotic change, and can be used to assess cardiovascular risk, consistent with ADMA being predominantly absorbed by endothelial cells. NO is an important messenger molecule involved in numerous biological processes, and its activity is essential to understand both pathogenic and therapeutic mechanisms in kidney disease and renal transplantation. NO production is reduced in renal patients because of their elevated ADMA levels with associated reduced DDAH activity. These factors contribute to endothelial dysfunction, oxidative stress and the progression of renal damage, but there are treatments that may effectively reduce ADMA levels in patients with kidney disease. Available data on ADMA levels in controls and renal patients, both in adults and children, also are summarized in this review. 相似文献
11.
The chemical stability of perfluorinated and non‐perfluorinated low temperature fuel cell model compounds (MCs) against attack by hydroxyl radicals, HO•, is compared using a competition kinetics approach in aqueous solutions at ambient temperature. HO• radicals were generated in situ by UV photolysis of hydrogen peroxide in the electron spin resonance (ESR) resonator. Acetic acid (AA), trifluoroacetic acid (TFAA), methanesulfonic acid (MSA), trifluorosulfonic acid (TFSA), and perfluoro(2‐ethoxyethane)sulfonic acid (PFEESA) were chosen as MCs, while the rate constants of 5,5‐dimethyl‐1‐pyrroline‐N‐oxide (DMPO) and methanol (CH3OH) served as reference for the determination of relative rate constants by means of steady state ESR signal amplitudes. In decreasing order the rate constants are: kMSA = (4.8 ± 0.2) × 107 M–1 s–1, kAA = (4.2 ± 0.3) × 107 M–1 s–1, kPFEESA = (3.7 ± 0.1) × 106 M–1 s–1, kTFAA = (7.9 ± 0.2) × 105 M–1 s–1, and kTFSA < 1.0 × 105 M–1 s–1. Applying these results to perfluorinated fuel cell membranes like Nafion®, the main points of attack by HO• are concluded to be the ether groups of the side chains, followed by the remaining carboxyl groups from the manufacturing process of the polymers. 相似文献
12.
Determination of Ni and Zn in an Oil Matrix using Schiff Base‐Assisted Extraction Followed by a Flame Atomic Absorption Spectrometer: A Simple Strategy to Determine Ni and Zn
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Arda Karan Feyzullah Tokay Sema Bağdat 《Journal of the American Oil Chemists' Society》2018,95(7):769-777
In the present work, a new and simple Schiff base‐assisted extraction strategy for Ni and Zn from an edible oil matrix with subsequent determination using a flame atomic absorption spectrometer was suggested. According to the green approach, laborious sample‐pretreatment procedures were eliminated via complexation of the analytes with N,N′‐bis(4‐methoxysalycylidene)‐2‐hydroxy‐1,3‐propanediamine (4MSHP) and transferred from the oil phase to the aqueous phase. The complexation properties of 4MSHP, Ni, and Zn were investigated using UV–vis spectrophotometry. The experimental conditions that affect the extraction efficiency were optimized using central composite design. The optimum conditions for the extraction of Ni and Zn were as follows: a volume to oil mass ratio of 0.83 to 1.31 mL g?1 of 4MSHP solution; 62.3‐ and 50.6‐min, stirring time; 27.3 and 31.1 °C, temperature, respectively. The detection limits (3sbm?1) were 0.41 μg g?1 for Ni and 0.16 μg g?1 for Zn. Validation of the suggested work was performed by the analysis of organometallic standard‐doped n‐hexane solutions as certified reference materials under the optimum experimental conditions. The recovery percentages were warranted the accuracy and found as 98.2 ± 1.8% for Ni and 99.8 ± 1.2% for Zn. In addition, relative SD values were below 5% for both the analytes. The Student's t‐test showed that there was no significant difference between the found and doped amount of analytes at 95% confidence level. The features such as the detection technique, cheapness, eco‐friendly solvent usage, and practicality were better compared to the literature. 相似文献
13.
Aleksandra Kapuścik Dr. Pavel Hrouzek Dr. Marek Kuzma Simona Bártová Dr. Petr Novák Dr. Jouni Jokela Maren Pflüger Dr. Andreas Eger Dr. Harald Hundsberger Dr. Jiří Kopecký 《Chembiochem : a European journal of chemical biology》2013,14(17):2329-2337
Aeruginosin‐865 (Aer‐865), isolated from terrestrial cyanobacterium Nostoc sp. Luke?ová 30/93, is the first aeruginosin‐type peptide containing both a fatty acid and a carbohydrate moiety, and is the first aeruginosin to be found in the genus Nostoc. Mass spectrometry, chemical and spectroscopic analysis as well as one‐ and two‐dimensional NMR and chiral HPLC analysis of Marfey derivatives were applied to determine the peptidic sequence: D ‐Hpla, D ‐Leu, 5‐OH‐Choi, Agma, with hexanoic and mannopyranosyl uronic acid moieties linked to Choi. We used an AlphaLISA assay to measure the levels of proinflammatory mediators IL‐8 and ICAM‐1 in hTNF‐α‐stimulated HLMVECs. Aer‐865 showed significant reduction of both: with EC50 values of (3.5±1.5) μg mL?1 ((4.0±1.7) μM ) and (50.0±13.4) μg mL?1 ((57.8±15.5) μM ), respectively. Confocal laser scanning microscopy revealed that the anti‐inflammatory effect of Aer‐865 was directly associated with inhibition of NF‐κB translocation to the nucleus. Moreover, Aer‐865 did not show any cytotoxic effect. 相似文献
14.
Karin S. Altmann Dr. Antje Havemeyer Prof. Dr. Eric Beitz Prof. Dr. Bernd Clement 《Chembiochem : a European journal of chemical biology》2012,13(17):2599-2604
Free endogenous methylarginines, Nω‐monomethyl‐L ‐arginine (L ‐NMMA) and Nω,Nω′‐dimethyl‐L ‐arginine (ADMA), inhibit NO synthases (NOSs) and are metabolized by dimethylargininedimethylaminohydrolase (DDAH). A postulated metabolism has been shown several times for DDAH‐1, but the involvement of DDAH‐2 in the degradation of ADMA and L ‐NMMA is still a matter of debate. Determination of the isoform‐specific DDAH protein expression profiles in various porcine tissue types shows a correlation of DDAH activity only with DDAH‐1 levels. DDAH activity (measured as L ‐citrulline formation from the conversion of methylarginines and alternative DDAH substrates) was detected in DDAH‐1‐rich porcine tissue types, that is, kidney, liver, and brain, but not in DDAH‐2‐rich porcine fractions, that is, spleen and thyroid. Furthermore, several ex vivo studies showed DDAH activity to be important for L ‐citrulline formation in porcine tissue and indicated the absence of an endogenous DDAH inhibitor in porcine tissue. This study provides new insights into tissue distributions as well as substrate selectivity for both DDAH isoforms. Although DDAH‐1 is known to metabolize the endogenous NOS inhibitors L ‐NMMA and ADMA, a physiological function for DDAH‐2 has yet to be determined. Hence, determining DDAH activity by methylarginine conversion is not suitable for analyzing isoform selectivity of DDAH‐1 inhibitors as postulated. 相似文献
15.
Chetna Jadala Manda Sathish Pratibha Anchi Ramya Tokala Uppu Jaya Lakshmi Velma Ganga Reddy Dr. Nagula Shankaraiah Dr. Chandraiah Godugu Dr. Ahmed Kamal 《ChemMedChem》2019,14(24):2052-2060
Molecular hybridization approach is a promising structural modification tool to design new chemical entities (NCEs) by mimicking two different pharmacophoric units into one scaffold to enhance the biological properties. With this aim, combretastatin-A4 acids were integrated with sulfonyl piperazine scaffolds as a one molecular platform and evaluated for their in vitro antiproliferative activity against a panel of human cancer lines cell lines namely, lung (A549), mouse melanoma (B16F10), breast (MDA MB-231and MCF-7) and colon (HCT-15) by MTT assay. Amongst which the compound (E)-3-(4-Chlorophenyl)-1-(4-((4-chlorophenyl)sulfonyl)piperazin-1-yl)-2-(3,4,5-trimethoxyphenyl)prop-2-en-1-one ( 5 ab ) displayed significant IC50 values in the range of 0.36 to 7.08 μm against the selected cancer cell lines. Moreover, 5 ab was found to be the most potent member of this series with IC50 0.36±0.02 μm . Further investigations revealed that the compound 5 ab displayed significant inhibition of tubulin assembly with IC50 5.24±0.06 μm and molecular docking studies also disclosed the binding of 5 ab effectively in CA4 binding space at the colchicine binding site. The flow cytometric analysis demonstrated that the compound 5 ab caused cell cycle arrest at G2/M phase in A549 cells. Compound 5 ab induced apoptosis in A549 cells which was further evaluated by different staining assays such as DAPI and AO which undoubtedly speculated, the induction of apoptosis. To study the anti-migration with 5 ab , cell migration/scratch wound assay was performed and the extent of apoptosis was studied by Annexin-V, including mitochondrial potential by JC-1 staining. 相似文献
16.
Kalpana Tilekar Neha Upadhyay Prof. Franz-Josef Meyer-Almes Dr. Fulvio Loiodice Natalia Y. Anisimova Tatiana S. Spirina Darina V. Sokolova Galina B. Smirnova Dr. Jun-yong Choe Dr. Vadim S. Pokrovsky Prof. Antonio Lavecchia Prof. C S Ramaa 《ChemMedChem》2020,15(19):1813-1825
In search of novel and effective antitumor agents, pyrazoline-substituted pyrrolidine-2,5-dione hybrids were designed, synthesized and evaluated in silico, in vitro and in vivo for anticancer efficacy. All the compounds exhibited remarkable cytotoxic effects in MCF7 and HT29 cells. The excellent antiproliferative activity toward MCF7 (IC50=0.78±0.01 μM), HT29 (IC50=0.92±0.15 μM) and K562 (IC50=47.25±1.24 μM) cell lines, prompted us to further investigate the antitumor effects of the best compound S2 (1-(2-(3-(4-fluorophenyl)-5-(p-tolyl)-4,5-dihydro-1H-pyrazol-1-yl)-2-oxoethyl)pyrrolidine-2,5-dione). In cell-cycle analysis, S2 was found to disrupt the growth phases with increased cell population in G1/G0 phase and decreased cell population in G2/M phase. The excellent in vitro effects were also supported by inhibition of anti-apoptotic protein Bcl-2. In vivo tumor regression studies of S2 in HT29 xenograft nude mice, exhibited equivalent and promising tumor regression with maximum TGI, 66 % (i. p. route) and 60 % (oral route) at 50 mg kg−1 dose by both the routes, indicating oral bioavailability and antitumor efficacy. These findings advocate that hybridization of pyrazoline and pyrrolidine-2,5-dioes holds promise for the development of more potent and less toxic anticancer agents. 相似文献
17.
Comparison of the performance of copper oxide and yttrium oxide nanoparticle based hydroxylethyl cellulose electrolytes for supercapacitors
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Biodegradable solid polymer electrolyte (SPE) systems composed of hydroxylethyl cellulose blended with copper(II) oxide (CuO) and yttrium(III) oxide (Y2O3) nanoparticles as fillers, magnesium trifluoromethane sulfonate salt, and 1‐ethyl‐3‐methylimidazolium trifluoromethane sulfonate ionic liquid were prepared, and the effects of the incorporation of CuO and Y2O3 nanoparticles on the performance of the SPEs for electric double‐layer capacitors (EDLCs) were compared. The X‐ray diffraction results reveal that the crystallinity of the SPE complex decreased upon inclusion of the Y2O3 nanoparticles compared to CuO nanoparticles; this led to a higher ionic conductivity of the Y2O3‐based SPE [(3.08 ± 0.01) × 10?4 S/cm] as compared to CuO [(2.03 ± 0.01) × 10?4 S/cm]. The EDLC performances demonstrated that the cell based on CuO nanoparticles had superior performance in terms of the specific capacitance, energy, and power density compared to the Y2O3‐nanoparticle‐based cell. However, Y2O3‐nanoparticle‐based cell displayed a high cyclic retention (91.32%) compared to the CuO‐nanoparticle‐based cell (80.46%) after 3000 charge–discharge cycles. © 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2017 , 134 , 44636. 相似文献
18.
Manfred Schrewe Anders O. Magnusson Christian Willrodt Bruno Bühler Andreas Schmid 《Advanced Synthesis \u0026amp; Catalysis》2011,353(18):3485-3495
The alkane monooxygenase AlkBGT from Pseudomonas putida GPo1 constitutes a versatile enzyme system for the ω‐oxyfunctionalization of medium chain‐length alkanes. In this study, recombinant Escherichia coli W3110 expressing alkBGT was investigated as whole‐cell catalyst for the regioselective biooxidation of fatty acid methyl esters to terminal alcohols. The ω‐functionalized products are of general economic interest, serving as building blocks for polymer synthesis. The whole‐cell catalysts proved to functionalize fatty acid methyl esters with a medium length alkyl chain specifically at the ω‐position. The highest specific hydroxylation activity of 104 U gCDW−1 was obtained with nonanoic acid methyl ester as substrate using resting cells of E. coli W3110 (pBT10). In an optimized set‐up, maximal 9‐hydroxynonanoic acid methyl ester yields of 95% were achieved. For this specific substrate, apparent whole‐cell kinetic parameters were determined with a Vmax of 204±9 U gCDW−1, a substrate uptake constant (KS) of 142±17 μM, and a specificity constant Vmax/KS of 1.4 U gCDW−1 μM −1 for the formation of the terminal alcohol. The same E. coli strain carrying additional alk genes showed a different substrate selectivity. A comparison of biocatalysis with whole cells and enriched enzyme preparations showed that both substrate availability and enzyme specificity control the efficiency of the whole‐cell bioconversion of the longer and more hydrophobic substrate dodecanoic acid methyl ester. The efficient coupling of redox cofactor oxidation and product formation, as determined in vitro, combined with the high in vivo activities make E. coli W3110 (pBT10) a promising biocatalyst for the preparative synthesis of terminally functionalized fatty acid methyl esters. 相似文献
19.
Janna Geith ThomasM. Klaptke Jan Weigand Gerhard Holl 《Propellants, Explosives, Pyrotechnics》2004,29(1):3-8
The enthalpies of combustion (ΔcombH) of dinitrobiuret (DNB) and diaminotetrazolium nitrate (HDAT‐NO3) were determined experimentally using oxygen bomb calorimetry: ΔcombH(DNB)=5195±200 kJ kg−1, ΔcombH(HDAT‐NO3)=7900±300 kJ kg−1. The standard enthalpies of formation (ΔfH°) of DNB and HDAT‐NO3 were obtained on the basis of quantum chemical computations at the electron‐correlated ab initio MP2 (second order Møller‐Plesset perturbation theory) level of theory using a correlation consistent double‐zeta basis set (cc‐pVTZ): ΔfH°(DNB)=−353 kJ mol−1, −1 829 kJ kg−1; ΔfH°(HDAT‐NO3)=+254 kJ mol−1, +1 558 kJ kg−1. The detonation velocities (D) and detonation pressures (P) of DNB and HDAT‐NO3 were calculated using the empirical equations by Kamlet and Jacobs: D(DNB)=8.66 mm μs−1, P(DNB)=33.9 GPa, D(HDAT‐NO3)=8.77 mm μs−1, P(HDAT‐NO3)=33.3 GPa. 相似文献
20.
Zwitterion (Z) monomer 3‐[diallyl{3‐(diethoxyphosphoryl)propyl}ammonio]propane‐1‐sulfonate underwent cyclocopolymerization with sulfur dioxide to give a new alternating copolymer poly(Z‐alt‐SO2) in excellent yield (ca 90%). The polyzwitterion (±) (PZ) (i.e. poly(Z‐alt‐SO2), bearing a diethylphosphonate as well as a sulfonate functionality in each repeat unit, upon ester hydrolysis gave its corresponding pH‐responsive polyzwitterionic acid (±) (PZA). The pH‐induced equilibrations (+) cationic polyelectrolyte ? (±) PZA ? polyzwitterion/anion (± ?) (PZAN) ? polyzwitterion/dianion (± =) (PZDAN) permitted us to examine the effects of charge types and their densities on the interesting solubility and viscosity behaviours. The apparent protonation constants of the basic functionalities &tbond;N±PO32? in (± =) PZDAN and &tbond;N±PO3H1? in (± ?) PZAN in salt‐free water and 0.1 mol L?1 NaCl were determined using potentiometric titrations. (±) PZA at a meagre concentration of 20 ppm was found to be an effective antiscalant to inhibit the precipitation of CaSO4 from its supersaturated solution: after 500 and 800 min, the respective scale inhibitions of 86 and 98% indicated its potential use as an effective antiscalant in reverse osmosis plant. © 2014 Society of Chemical Industry 相似文献