酶联免疫吸附法在食品安全性指标检测中的 研究进展

随着我国社会经济的快速发展和人们生活水平的提高,食品安全问题日益成为人们关注的焦点。酶联免疫吸附法是在免疫学和细胞工程学基础上发展的一种微量检测技术,具有操作简便、灵敏度高等优点。本文总结了酶联免疫吸附法在农药残留、兽药残留、致病微生物、生物毒素、非法添加的非食用物质、重金属、过敏原和转基因食品等检测中的研究进展,评价了该检测方法的优缺点,并对其未来的发展方向进行了展望和建议。     

酶联免疫吸附技术及其在食品安全检测中的应用

主要综述酶联免疫吸附技术及其在农药残留、食品中违法添加的非食用物质、食品中生物毒素、食品中病原微生物、转基因食品等食品检测中的应用,并根据当前研究现状进行了展望。     

酶联免疫吸附法及其在食品分析中的应用

简单介绍了酶联免疫吸附法(ELISA),并且就其在食品分析中的应用进行了较详细的评述,主要包括ELISA用于食品微生物、食品中的毒素、残留农药和其他成分的检测。     

酶联免疫吸附法在食品检验中的应用进展

在食品安全管理方面,需要采用有效手段进行食品检验。基于此,本文对灵敏度高、特异性强和自动化程度高的酶联免疫吸附法展开了分析,并对该方法在食品农兽药残留、微生物和毒素检验中的应用进展进行了探讨,可为关注这一话题的人们提供参考。     

酶联免疫吸附法及其在食品分析中的应用

简单介绍了酶联免疫吸附法 (ELISA) ,并且就其在食品分析中的应用进行了较详细的评述 ,主要包括ELISA用于食品微生物、食品中的毒素、残留农药和其他成分的检测      

酶联免疫吸附法(ELISA)在乳制品检测中的应用

简单介绍了酶联免疫吸附法（ＥＬＩＳＡ）,并且就其在乳制品检测中的应用进行了较详细的评述,主要包括ＥＬＩＳＡ用于乳制品中的免疫球蛋白、乳铁蛋白和其他成分的测定。     

酶联免疫吸附法在植物性食品安全检测中的应用

文章概述了酶联免疫吸附法（ELISA）的基本原理及方法，详述了其在植物性食品安全检测中的应用，并对该方法的发展趋势及其在食品安全检测中的应用前景进行了展望。     

酶联免疫吸附法在沙门氏菌检测中的研究进展

酶联免疫吸附技术(ELISA)作为先进的免疫化学检测技术正越来越广泛地应用于沙门氏菌检测中.文中介绍了沙门氏菌的特性及其主要危害,阐述了ELISA的基本原理,并结合国内外研究成果,综述了目前ELISA法在沙门氏菌检测中几种常用的方法,主要有:直接ELISA法、竞争ELISA法、Dot-ELISA法、LPS-ELISA法和PCR-ELISA法,从而表明了在沙门氏菌检测中ELISA法具有良好的特异性和敏感性.     

免疫学检测肉类制品掺假研究进展

由于价格、原料、过敏原和宗教等原因,市场上肉类掺假的现象非常普遍。针对这种肉类掺假产生了多种检测方法,本文主要综述了以ELISA为主的免疫学快速检测方法。     

肉类掺假鉴别技术研究进展

随着现代分析检测技术的发展,肉类掺假鉴别技术也得到了长足的发展,主要包括感官鉴定技术、酶联免疫吸附(enzyme-linked immunosorbent assay,ELISA)技术、聚合酶链式反应(polymerase chain reaction,PCR)技术及生物质谱技术等。在这些技术中,感官鉴定技术操作简单,但准确度还有待改进。ELISA技术已有商品化试剂盒,由于受蛋白活性的影响,使其应用范围受到限制。PCR技术具有检测灵敏度高、结果重复性好等优势,但操作繁琐耗时。生物质谱技术通过多肽鉴定肉类掺假成为了一个新的发展方向。本文综述了现阶段常见肉类成分的检测技术,并对各种肉类掺假鉴别技术进行总结与分析,期望为肉类食品安全监测提供理论参考。     

Improved ELISA and dot-blot methods for the detection of whey proteins in meat products

Two techniques, ELISA and dot-blot, were applied to the qualitative detection of very low levels of whey proteins in liver pãtés. The use of an avidin-biotin amplification system for both methods led to a useful improvement of the detection limit. The detection level which was 4 g kg^?1 with the classical ELISA method was improved to I g kg^?1 with the amplified ELISA method. Using the dot-blot technique, the results showed that the minimum detectable level was 1–7 g kg^?1 for the classical method with nitrocellulose (NC), 0–7 g kg^?1 for the amplified method with NC, 0–7 g kg^?1 for the classical method with cyanogen bromide-activated NC (activated NC) and 0–3 g kg^?1 for the amplified method with activated NC.     

Species identification of meat products by ELISA

ELISA methods used in this study are proved to detect low contents of animal species (pork, beef, sheep and poultry), even in highly processed foods. They present the advantages of being robust, cheap and easy to perform. Nevertheless, F factors, determining the threshold values of the test, need to be validated for each species.     

Double-antibody ELISA for detection of trace amounts of pig meat in raw meat mixtures

Pre-rigor cooked beef is tender if the cooking produces severe shortening. This study was conducted to compare the effects of different heating rates on shortening and tenderness. Myofibrillar and cooking shortening and related changes were measured with physiograph recordings on pre-rigor M. triceps brachii strips suspended in paraffin oil during heating. Warner-Bratzler shear values were determined on M. triceps brachii samples heated at approximately the same rates at which the muscle strips were heated. Rapid heating (2°C/2min) produced more (p < 0·01) severe myofibrillar shortening that was complete at higher (p < 0·01) muscle temperatures than slow heating (2°C/12 min). Regardless of animal age, rapid heating resulted in a cooked product that was more (p < 0·01) tender than that produced by slow heating in the pre-rigor state and slow heating resulted in a more (p < 0·01) tender product than that achieved by rapid heating in the post-rigor state. Data on muscle shortening and from differential scanning calorimetry suggest that the tenderness produced from pre-rigor rapid heating results from a heat-induced active contraction.     

DNA条形码技术在肉及其制品中的应用

DNA条形码技术作为一种新的分子生物学检测技术在鉴定和区分各物种和物种间亲缘关系方面得到了广泛应用,该技术能实现对肉的快速、准确检测。DNA条形码已成为生物学领域发展最迅速的一种技术,该技术基于广泛的物种基因数据库信息,在肉品研究中具有良好的应用前景。本文简述DNA条形码技术的基本原理,并基于DNA水平上与其他相关技术进行比较,综述其在物种鉴定、肉品质量安全、商业欺诈等方面的应用,并对该技术在今后肉品科学研究中的应用进行展望。      

注水肉检测方法研究进展

注水肉不仅是以水充肉、缺斤少两的经济问题,更是食品安全问题,关系到公共健康。综述了多种常见的检测方法,如感官判断、试纸法、快速检测仪器法、干燥法及低场核磁共振分析法等。     

"瘦肉精"残留的危害与检测方法分析

主要介绍了"瘦肉精"的来源、作用机理、危害和检测方法,并对其监管过程中存在的问题进行了总结,以使消费者了解"瘦肉精"的危害.     

Sandwich ELISA for detection of horse meat in raw meat mixtures using antisera to muscle soluble proteins

A double-antibody sandwich ELISA (enzyme-linked immunosorbent assay) has been successfully developed for the detection of defined amounts of horse meat (1-50%) in unheated meat mixtures. The assay uses horse-specific antibodies obtained by immunoadsorption of the crude horse antisera onto immobilised sarcoplasmic extracts from chicken, beef and pig to remove cross-reacting antibodies. The purified antibodies bound to a solid support sequester horse muscle soluble proteins from meat mixtures. Further immunorecognition was made with the same antibodies conjugated to the enzyme horseradish peroxidase. Subsequent enzymic conversion of substrate gave clear optical density differences when assaying mixtures of minced beef and pig containing variable amounts of horse meat.     

Production of a horse-specific monoclonal antibody and detection of horse meat in raw meat mixtures by an indirect ELISA

A stable hybridoma cell line (DD3) has been produced secreting a monoclonal antibody specific for horse muscle proteins. The DD3 monoclonal antibody (mAb) did not show significant cross-reactivity when tested against beef, chicken, pig and soya proteins or bovine caseins, gelatine and bovine serum albumin. The DD3 mAb was used in an indirect ELISA format for the detection of defined amounts of horse meat (10–500 g kg-¹) in beef meat mixtures. Immunorecognition of monoclonal antibodies adsorbed to horse meat adsorbed onto the ELISA plate was made with rabbit anti-mouse immunoglobulins conjugated to the enzyme horseradish peroxidase. Subsequent enzymic conversion of the substrate gave clear optical density differences when assaying mixtures of minced beef containing different amounts of horse meat.