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1.
Two unusual lipid classes were detected by thin-layer chromatography in the neutral lipids derived from goat cauda-epididymal
sperm plasma membrane. The lipids were identified as wax esters and 1-O-alkyl-2,3-diacylglycerols based on chromatographic properties, identity of their hydrolysis products, and infrared/1H nuclear magnetic resonance spectral evidence. The membrane containedca. 3 and 5 μg/mg protein of wax esters and alkyldiacylglycerols, respectively. The relative proportions of wax esters and alkyldiacylglycerols
in the total neutral lipids were 1.5% and 2.4%, respectively. The lipids contained fatty acids with chain lengths of C14 to C22. The major fatty acids of the wax esters were 14∶0, 16∶0, 16∶1ω7, 18∶0 and 18∶1ω9. The fatty acids in alkyldiacylglycerol
were 16∶0, 18∶0, 22∶5ω3 and 22∶6ω3. Alkyldiacylglycerol was particularly rich in docosahexaenoic acid 22∶6ω3) representing
30% of the total fatty acids. The alcohols of wax ester were all saturated with C20–C29 carbon chains. The deacylated products derived from alkyldiacylglycerols were identified as hexadecyl, octadecyl and octadec-9′-enyl
glycerol ethers. 相似文献
2.
Young male rats were fed ad libitum for 8 weeks a low iron fat-free (FF-Fe) diet or a fat-free diet supplemented with iron
(FF+Fe). The relative levels of 16∶1 to 16∶0 and 18∶1 to 18∶0 in the total fatty acids of liver and other tissues (plasma,
erythrocytes and intestinal mucosa) were considerably decreased because of a lack of dietary iron. In rats fed the FF-Fe diet,
the levels of essential fatty acids (18∶2ω6+20∶4ω6) in tissues were 2-to 3-fold greater than in the corresponding tissues
of rats fed the FF+Fe diet. Eicosatrienoic acid (20∶3ω9) levels in tissue lipids from rats fed the FF+Fe diet were high (8–16%),
whereas they were low (2–5%) in the case of animals fed the FF-Fe diet. The proportion of 20∶4 in total fatty acids of tissues
was 2-to 3-fold greater in rats fed the FF-Fe diet than when they were fed the FF+Fe diet. Therefore, the relative levels
of 20∶3ω9/20∶4ω6 varied from 1-2.9 in tissue lipids of rats fed the FF+Fe diet, while it varied only from 0.2–0.3 in animals
fed the FF-Fe diet. These results suggest that a lack of dietary iron may reduce the synthesis of 16∶1, 18∶1, 20∶3 and 20∶4
and the metabolism of 20∶4. 相似文献
3.
The lipid composition of six thermophilic fungi (Myriococcum albomyces, Mucor miehei, Papulaspora thermophila, Rhizopus sp.,Thielavia thermophila (+)Thielavia thermophila (−), andTorula thermophila) was examined. The relative per cent total lipids (4.9–26.3%), neutral lipids (55.5–88.3%), polar lipids (11.7–44.6%) and
the fatty acid profile of each lipid fraction was determined. The predominant fatty acids were 16∶0, 18∶0 and 18∶2, and lesser
amounts of 12∶0, 14∶0, 15∶0, 16∶1, 16∶2, 17∶0 and 18∶3 were present. The total lipids contained an average of 0.96 double
bonds per mole fatty acid (unsaturation index [USI]) the neutral lipids 0.86 USI and the polar lipids 0.84 USI, excluding
the values forTorula thermophila. These data show a high degree of saturation and are consistent with data reported for other fungal thermophiles.Torula thermophila possessed abnormally high USI values (1.15–1.50) and was cultured at three different temperatures (25, 45 and 51 C). As the
culture temperature ofTorula thermophila increased, the USI decreased. The USI of the polar lipids ofTorula thermophila at 25, 45 and 51 C were 1.50, 1.28 and 1.11, respectively. Thus the membrane lipids of this fungus appear unusual for a thermophile. 相似文献
4.
The total lipids and fatty acid composition ofEntomophthora coronata were determined. The fungus was grown on a chemically defined medium and a chemically nondefined medium (Sabouraud dextrose
yeast extract) for a period of 26 days. The organism contained from 16.2% to 44.6% total lipids depending upon the days of
growth. The major fatty acids were 12∶0 (5.5–9.0%), 13∶0 (1.2–8.2%), 14∶0 (33.5–43.5%), 16∶0 (9.7–13.9%), 18∶19 (20.4–22.4%), and 18∶29,12 (3.5–10.5%). Lesser amounts of 15∶0, 16∶1, 16∶2, 17∶0, 18∶0, two other 18∶2 (both having conjugated double bonds), 18∶36,9,12, another 18∶3 (conjugated double bonds), 20∶38,11,14, 20∶45,8,11,14, another 20∶4 (conjugated double bonds), and 24∶1 acids were found. Trace amounts of 20∶0, 20∶1, 20∶2, 22∶0 and 24∶0 were
also present. The relative percentage of most of the fatty acids did not vary appreciably with growth. However, 18∶29,12 and 20∶45,8,11,14 increased with age of the chemically defined culture. Peak E (18∶2, conjugated double bonds) increased and 13∶0 and 18∶36,9,12 decreased with age of the chemically nondefined culture. The fatty acids were predominately saturated (56.9–69.1%) and contained
a high percentage of shorter chain fatty acids (C 12 to C 15). The fatty acids of the chemically defined culture were more
unsaturated than the Sabouraud culture and the unsaturation increased with age of the culture. 相似文献
5.
The fatty chain compositions of 1-O-alk-1′-enyl-2-acyl, 1-0-alkyl-2-acyl, and 1,2-diacyl glycerophospholipids of the Japanese oysterCrassostrea gigas (Thunberg) were investigated. Major fatty chains in thesn-1 position of 1-alk-1′-enyl-2-acyl ethanolamine phospholipids (EPL) were 18∶0 (64.7%) and 20∶1 (11.1%). Majorsn-1 chains of alkenylacyl choline phospholipids (CPL) were 18∶0 (63.3%) and 16∶0 (22.2%). In the case of 1-alkyl-2-acyl EPL,
the predominant fatty chains in thesn-1 position were 18∶0 (51.5%), 16∶0 (16.0%) and 20∶1 (12.5%); in the case of 1-alkyl-2-acyl CPL, the majorsn-1 chains were 16∶0 (44.0%) and 14∶0 (23.4%). Saturated fatty chains were predominant in both EPL and CPL. Prominent fatty
acids in thesn-2 position of the alkenylacyl EPL were 22∶6n−3 (29.0%), 20∶5n−3 (19.0%) and 22∶2 NMID (non-methylene interrupted dienes,
16.6%) contributing to about 65% of the total fatty acids, while alkenylacyl CPL was rich in the saturated acids 16∶0 (32.0%)
and 18∶0 (9.2%). In the alkylacyl EPL, 16∶0, 18∶1n−9, 18∶0 and 16∶1n−7 were prominentsn-2 fatty acids and accounted for 30.6%, 10.0%, 9.8%, and 8.3%, respectively. Polyunsaturated fatty acids were detected, but
were present at extremely low percentages. Majorsn-2 fatty acids in alkylacyl CPL were 16∶0 (25.4%), 22∶6n−3 (16.0%) and 20∶5n−3 (8.4%). The major fatty acids of diacyl EPL
were 20∶5n−3 (22.3%), 16∶0 (17.9%), and 18∶0 (16.1%), and those of diacyl CPL were 16∶0 (30.4%), 20∶5n−3 (17.6%) and 18∶1n−7
(7.4%). 相似文献
6.
Ricardo J. Pollero 《Lipids》1983,18(1):12-17
Neutral lipid, phospholipids and fatty acids of the sea anemonePhymactis clematis from the south-west Atlantic were characterized and quantified in spring and autumn. Neutral lipids predominated over phospholipids
in both seasons. Triacylglycerol and diacylglycerol ethers were the major lipids. In spring, an increase of esterified sterols
was noted. The major fatty acids found were 22∶5ω3, 20∶5ω3 and 16∶0. The sea anemones were also incubated in vivo with either
[1-14C]linoleate or [1-14C] α-linolenate for 2 hr. Isotope incorporation into lipids and their transformations into higher fatty acids were examined.
Both precursors were incorporated into the lipids, mainly in triacylglycerols and mono-acylglycerols, while α-linolenate was
also incorporated into phospholipids. The radioactive linoleate was elongated to 20∶2, 22∶2 and 24∶2 fatty acids, but not
desaturated to 18∶3ω6. α-Linolenate was desaturated by Δ6 desaturase to 18∶4ω3. The specificity of Δ6-desaturase is discussed. 相似文献
7.
The fatty acid composition of three unicellular algal species used as food sources for larvae of the American oyster (Crassostrea virginica) 总被引:1,自引:0,他引:1
The total lipid and fatty acid content of 3 algal species,Pyramimonas virginica, Pseudoisochrysis paradoxa andChlorella sp., which have been successful as food sources for rearing larvae of the American oyster,Crassostrea virginica, was determined. Of the fatty acids of ω6 and ω3 families which have been shown to be essential fatty acids for normal growth
in many animals, only the ω6 fatty acids were found to be higher in these 3 species of algae than in the traditional oyster
larvae diet which consists of the algaeMonochrysis lutheri andIsochrysis galbana. The major fatty acid constituents of the total lipids of the 3 species were the C12, C14, C16 and C18 saturated fatty acids
and the C16 and C18 mono- and polyunsaturated acids. These components constituted 70–93% of the total lipid in cultures of
all ages. There were modest amounts of C20 and C22 polyunsaturated acids; some of these existed only in trace amounts. InP. virginica andChlorella sp., hexadecanoic acid was dominant (23–39%). The presence of large quantities of tetradecanoic acid (22–26%) and oleic acid
(17–21%) was characteristic ofP. paradoxa. Chlorella sp. had the highest proportion of octadecatrienoic acid (18∶3ω3) which accounted for up to 17% of the total lipids. γ-Linolenic
acid (18∶3ω6) was found only inChlorella sp., but in the 5th-day culture only. The lowest proportion of total polyethylenic acid was inP. paradoxa; however, lipid analyses showed this alga had the most lipid/individual cell. Some variations were observed in the fatty
acid composition with age of the culture.
Contribution No. 883 of the Virginia Institute of Marine Science, Gloucester Point, VA 23062. 相似文献
8.
Lih-Ling Wang Andrew C. Peng Andrew Proctor 《Journal of the American Oil Chemists' Society》1990,67(8):499-502
Lipids from five cultivars of highbush blueberries (Vaccinium corymbosum L.) were extracted and fractionated into neutral lipids (60–66%), glycolipids (20–22%) and phospholipids (14–18%). The major
fatty acids in all fractions were palmitic (16∶0), oleic (18∶1), linoleic (18∶2), and linolenic (18∶3) acids. All lipid classes
had a large concentration of C18 polyunsaturated acids (84–92%), indicating that blueberries are a rich source of linoleic and linolenic acids. Changes in
the fatty acid composition of neutral lipids and phospholipids were not significantly different among the five cultivars,
but significant differences were noted in the ratios of linoleic and linolenic acids in the glycolipids fraction. 相似文献
9.
The lipid composition of the yellow clam,Mesodesma mactroides, that lives in the northern beaches of the Buenos Aires province of Argentina was studied. The main nonpolar lipids are triglycerides
and alkoxyglycerides. Phosphatidyl choline, phosphatidyl ethanolamine, and phosphatidyl serine are the main phospholipids.
The predominant fatty acids are 16∶0, 16∶1ω7, 18∶0, 18∶1ω9, 20∶5ω3, and 22∶6ω3. The are mainly provided by the clam's food
and stored in the hepatopancreas. The content of polyunsaturated acids increases in summer together with an increase in nonpolar
lipids and is correlative with an increase in phytoplankton in the sea water. Sexual maturity modifies the lipid composition
of gametes. 相似文献
10.
The activity of the overt form of rat liver mitochondrial carnitine palmitoyltrasferase or CPT0 (EC 2.3.1.21) towards different fatty acid substrates was studied. The following non-esterified fatty acids (NEFA) and their
CoA esters in the presence of 1% bovine serum albumin (BSA) were tested: 16∶0, 18∶0, 18∶1, 18∶2, 18∶3ω3, 20∶4, 20∶5ω3 and
22∶6ω3. The data fit a square hyperbolic model for enzyme catalysis (p<0.001, non-linear regression). Asymptotic Vmax and K0.5, substrate concentration at one-half Vmax, were calculated using total concentrations of acyl-CoA, or unbound concentrations of NEFA. BSA was found to act as a true
substrate reservoir for NEFA in that the dissociation of the NEFA-BSA complex was 10–330 times faster than the CPT0 reaction. Regardless of form (NEFA or CoA ester), 18∶3ω3 gave the highest, while 22∶6ω3 and 18∶0 gave the lowest rates of
acylcarnitine synthesis. Except for 18∶3ω3 and 18∶2, Vmax for NEFA was generally lower than for acyl-CoA, with the greates differences observed for 20∶4, 20∶5ω3 and 22∶6ω3, suggesting
that acyl-CoA synthesis may also be important in the control of the entry of these fatty acids into the mitochondria. The
data provide an enzymatic rationale for the relatively low content of 18∶3ω3 in esterified lipid. 相似文献
11.
Milk fatty acids consist of about 20–25% palmitic acid (16∶0), with about 70% of 16∶0 esterified to thesn-2 position of the milk triacylglycerols. Hydrolysis of dietary triacylglycerols by endogenous lipases producessn-2 monoacylglycerols and free fatty acids, which are absorbed, reesterified, and then secreted into plasma. Unesterified 16∶0
is not well absorbed and readily forms soaps with calcium in the intestine. The positioning of 16∶0 at thesn-2 position of milk triacylglycerols could explain the high coefficient of absorption of milk fat. However, the milk lipase,
bile salt-stimulated lipase, has been suggested to complete the hydrolysis of milk fat to free fatty acids and glycerol. These
studies determined whether 16∶0 is absorbed from human milk assn-2 monopalmitin by comparison of the plasma triacylglycerol total andsn-2 position fatty acid composition between breast-fed and formula-fed term gestation infants. The human milk and formula had
21.0 and 22.3% of 16∶0, respectively, with 54.2 and 4.8% 16∶0 in the fatty acids esterified to the 2 position. The plasma
triacylglycerol total fatty acids had 26.0±0.6 and 26.2±0.6% of 16∶0, and thesn-2 position fatty acids had 23.3±3.3 and 7.4±0.7% of 16∶0 in the three-month-old exclusively breast-fed (n=17) and formula-fed
(n=18) infants, respectively. Marked differences were found in the plasma total and the 2 position phospholipid percentage
of 20∶4ω6, i.e., 11.6±0.3 and 6.9±0.6 (total), 17.7±1.4 and 9.7±0.6 (sn-2 position) and percentage of 22∶6ω3, 4.6±0.3 and 2.1±0.3 (total), 5.6±0.6 and 2.0±0.2 (sn-2 position) for the breast-fed and formula-fed infants, respectively. These studies provide convincing evidence that 16∶0
is absorbed from human milk assn-2 monoacyl-glycerol. The metabolic significance of the differences in positional distribution of fatty acids in the plasma
lipids of breast-fed and formula-fed infants is not known. 相似文献
12.
Néstor M. Carballeira Anastacio Emiliano Anthony Sostre José A. Restituyo Ileana M. González Gretchen M. Colon Carmen G. Tosteson Thomas R. Tosteson 《Lipids》1998,33(6):627-632
The fatty acid composition of a Pseudomonas sp. (Alteromonas) and its host, the dinoflagellate Ostreopsis lenticularis, vectors in ciguatera fish poisoning, has been studied. The major fatty acids in O. lenticularis were 16∶0, 20∶5n-3, and 22∶6n-3, but 18∶2n-6, 18∶3n-3, and 18∶n-3 were also identified. In contrast to other dinoflagellates,
18∶5n-3 was not detected in O. lenticularis. Even-chain fatty acids such as 9–16∶1, 11–18∶1, and 13–20∶1 predominated in the Pseudomonas sp. from O. lenticularis, but 16–20% of (E)-11-methyl-12-octadecenoic acid was also identified. The chirality of the latter was confirmed by total synthesis (28% overall
yield) starting from oxacyclotridecan-2-one. The fatty acid compositions of two other Pseudomonas species, from the palytoxin-producing zoanthids Palythoa mamillosa and P. caribdea, were also studied and were similar to that of the Pseudomonas sp. from O. lenticularis. The possibility of using some of these fatty acids as chemotaxonomic lipids in identifying marine animals that consume toxic
dinoflagellates or zoanthids is discussed. 相似文献
13.
Thirteen-day old rats were given intracranial injections of 1-14C linolenic acid (allcis 9,12,15 octa decatrienoic acid) and were sacrificed after 8 hr. Analysis of brain fatty acids showed that 16∶0, 18∶0, 18∶1,
18∶3, 20∶3, 20∶4, 20∶5, 22∶5, and 22∶6 were labeled. The total fatty acid methyl esters were separated into classes according
to degree of unsaturation on a AgNO3∶SiO2 impregnated plate. The bands were scraped off and the eluted fatty acids were first analyzed by radiogas liquid chromatography
and then subjected to reductive ozonolysis to determine double bond position. The saturated acids, 16∶0, and 18∶0, as well
as the mono-unsaturated 18∶1, must have been formed from radioactive acetate produced by β oxidation of the injected linolenate.
Among the polyunsaturated fatty acids, the triene fraction was characterized and identified as 18∶3 ε3 (Δ9,12,15), the starting material, and 20∶3 ω3 (Δ11,14,17); the tetraene fraction was identified as 20∶4 ω3 (Δ8,11,14,17); the pentaene fraction was identified as 20∶5 ω3 (Δ5,8,11,14,17) and 22∶5 ω3 (Δ7,10,13,16,19); and, finally, the hexaene fraction was shown to be 22∶6 ω3 (Δ4,7,10,13,16,19). The biosynthesis of these ω3 family fatty acids in the brain in situ is discussed. 相似文献
14.
The unsaponifiable lipids and total fatty acids of a nonphotosynthetic diatom,Nitzschia alba, have been examined. The major fatty acids were found to be 14∶0, 16∶0, 18∶1, and 20∶5; small amounts of 15∶0, 16∶1, 18∶0,
18∶2, 18∶3, and 20∶4 acids also were present. The unsaponifiable lipids consisted mostly of sterols, with only traces (<0.1%)
of hydrocarbons (chiefly C16, C18, and C28 normal olefins). The sterols contained brassicasterol (major) and clionasterol (minor), as well as traces of an unidentified
sterol; clionasterol was present only in glycosidically bound form. 相似文献
15.
R. A. Gibson 《Lipids》1983,18(11):743-752
The fatty acid methyl esters obtained by the esterification of total lipids extracted from 24 species of fin fish and 4 species
of invertebrates caught in the rivers and coastal waters of southern Australia were analyzed by gas chromatography. The lipids
of most species contained significant levels of arachidonic acid (0.7–15.8%) as well as the more common marine polyunsaturate,
eicosapentaenoic acid (0.7–15.9%). The major ω6 fatty acid present in most species was 20∶4; however, other fatty acids of
this series, including 18∶2, 22∶4 and 22∶5, were present. The level of total ω6 fatty acids ranged from 3.9 to 22.3% of the
total lipid. In general, the level of total ω3 polyunsaturates was higher than the total ω6 fatty acids with levels of ω3
fatty acids ranging from 9.6 to 48.2%. Only 2 fish (barramundi and gurnard perch) had ω6/ω3 ratios greater than 1.0. Most
of the Australian species examined contained low levels of fat (0.5–7.8% of fresh weight). Two species examined, callop (freshwater)
and blue groper (marine) contained sufficient quantities of both fat (7.7 and 7.8%) and arachidonic acid (4.8 and 9.3%) to
warrant consideration for commercial exploitation. 相似文献
16.
Ozonolysis studies of the monoenes of the fatty chain types in lipids of steer meibomian gland excreta (meibum) have confirmed
earlier structural assignments based on gas liquid chromatography (GLC) retention data and have assisted in assigning complete
structures to a group of recently identified ω-hydroxy fatty acids. The ω-hydroxy acids include straight-chain monoenoic acids
(85%), saturated anteiso and iso acids (13%), monoenoic acids of the latter group (1%) and, finally, saturates of the normal
monoenoic acids (1%). All the fatty chains of meibum can be biosynthesized by a unified process of chain buildup to primary
chain lengths of 12∶0–20∶0 for the straight evens, with 16∶0 predominating, 13∶0–21∶0 for the straight odds with 17∶0 predominating,
i16∶0 to i28∶0 for the iso and ai17∶0 to ai29∶0 for the anteiso chain types; then Δ9 desaturation of each of these chain types;
and finally chain elongation of 1–10 C2 units. Some chain degradation may also occur. The meibum lipid components involved are unsubstituted fatty acids, α-OH fatty
acids, ω-OH fatty acids, fatty alcohols and some other lipid components incompletely characterized. The carbon skeletons are
straight even, straight odd, iso and anteiso except that the α-OH fatty acids are only straight even and straight odd and
these chains are not elongated. All fatty chains are almost entirely saturated and monoenoic, the polyenes occurring in only
trace amounts. Biosynthesis of the fatty chains of human meibum evidently occurs similarly, except that considerably more
18∶0 than 16∶0 fatty acids are built up by the fatty acid synthetase, before desaturation and extension. 相似文献
17.
Rhodotorula gracilis CFR-1 has been evaluated for its potential to produce lipids. The yeast lipids closely resembled palmolein, a liquid fraction
of palm oil. It contained 2.3–3% free fatty acids, 64.4% tri-, 23.1% di-, and 6.1% mono-acylglycerols, 94.2% neutral and 5.8%
polar lipids. Most abundant fatty acids were C18∶1, C16∶0, C18∶2 and C18∶0 (43.8, 28.5, 13.5 and 4.5%). All fatty acids, irrespective
of the levels, followed definite patterns of increase or decrease during the advancement of fermentation. A pincers-shaped
curve was obtained when the total saturation and unsaturation were plotted. Use of different glucose and molasses-based media
did not show any significant overall effect on saturation (34.4–39.5%) and unsaturation (60.4–65.3%). Desaturation of fatty
acids was found to be a metabolic function occurring in the process of cell maturation. 相似文献
18.
The lipid classes, fatty acids of total and individual lipids and sterols of Antarctic krill (Euphausia superba Dana) from two areas of the Antarctic Ocean were analyzed by thin layer chromatography (TLC), gas liquid chromatography (GLC)
and gas liquid chromatography/mass spectrometry (GLC/MS). Basic differences in the lipid composition of krill from the Scotia
Sea (caught in Dec. 1977) and krill from the Gerlache Strait (caught in Mar. 1981) were not observed. The main lipid classes
found were: phosphatidylcholine (PC) (33–36%), phosphatidylethanolamine (PE) (5–6%), triacylglycerol (TG) (33–40%), free fatty
acids (FFA) (8–16%) and sterols (1.4–1.7%). Wax esters and sterol esters were present only in traces. More than 50 fatty acids
could be identified using GLC/MS, the major ones being 14∶0, 16∶0, 16∶1(n−7), 18∶1(n−9), 18∶1(n−7), 20∶5(n−3) and 22∶6(n−3).
Phytanic acid was found in a concentration of 3% of total fatty acids. Short, medium-chain and hydroxy fatty acids (C≤10)
were not detectable. The sterol fraction consisted of cholesterol, desmosterol and 22-dehydrocholesterol. 相似文献
19.
The fatty acid composition of different parts of the moss,Mnium cuspidatum, which contains up to 35% arachidonic acid in its lipids, was studied through the annual cycle and especially during the
period of rapid development of the reproductive parts. The content of 20∶4ω6 was highest in summer and lowest in winter; but
for 20∶5ω3, the reverse was found. Levels of the acids, 20∶5ω3, 18∶3ω3 and 16∶3ω3 showed parallel fluctuations through the
seasons of the year, and functionally they may substitute for each other. In contrast, 20∶5ω6 is at a high level when 18∶2ω6
is low. The latter acid accumulates in storage or dormant tissue and may be a reserve to form arachidonic acid for specific
requirements in cell membranes when rapid growth resumes. 相似文献
20.
The fatty acid composition of oyster larvae at various stages, as well as of the algal diet, were determined by gas liquid
chromatography (GC). Saturated fatty acids are the major fatty acid components in all larval stages and account for 34–62%,
30–35% and 35–81% of the neutral, polar and total lipids of algal-fed larvae respectively. Weight percentage of saturated
fatty acid in “starved” larvae was consistently higher (63–81%) during the whole period. The total polyunsaturated fatty acids
were higher in the polar lipids than in the neutral lipids. The concentration of the ω3 fatty acids also was comparatively
higher in the polar lipids than in the neutral lipids. In the total and neutral lipid fractions, the weight percentage of
polyunsaturated and ω3 fatty acids was higher in the eyed than in the pre-eyed (pediveliger) larvae. Eicosapentaenoic acid
(20∶5ω3) and 22∶6ω3 were not detected in lipids of “starved” and young larvae. There was an accumulation of 20∶5ω3, 22∶6ω3,
and total ω3 fatty acids in the older larvae. Lipid classes were separated by thin layer chromatography (TLC). There was no
qualitative change in lipid composition during larval development, but a marked increased of triacylglycerol in larvae up
to the stage of maturation in algae-fed larvae.
Contribution number 1195 of the Virginia Institute of Marine Science, Gloucester Point, VA 23062 相似文献