Effects of La^3＋ on ATPase Activities of Plasma Membrane Vesicles Isolated from Casuarina Equisetifolia Seedlings under Acid Rain Stress

Asamainkindofmembranetransporter ,plasmamembraneATPasesfacilitatethetransportofminerals ,sugars,metabolites ,andothercompoundsacrossthemembraneofthecells ,onlybywhichcouldplantcellscommunicatewithenvironmenttotransfersubstanceandinformation ,whichisessentialforplantgrowthandcellularhomeostasis[1] .Astheexperimentmateri als ,thehighlypurifiedplasmamembranevesiclesfromCasuarinaequisetifoliaseedlingscultivatedunderartificialacidrainstress ,wereisolatedbyaqueoustwo-phasepartitioningmethods .Thest…     

Effects of Lanthanum and Europium on Rooting of Plantlet Eriobotrya Japonica Lindl. in vitro

ＲａｒｅＥａｒｔｈＥｌｅｍｅｎｔｓ (ＲＥＥｓ)ｈａｖｅｂｅｅｎｗｉｄｅｌｙｕｓｅｄｄｕｅｔｏｔｈｅｉｒｓｐｅｃｉａｌｅｌｅｃｔｒｉｃｓｔｒｕｃ ｔｕｒｅｓａｎｄｖａｒｉｅｔｉｅｓｏｆｂｉｏｌｏｇｉｃａｌａｃｔｉｖｉｔｙ .ＩｎＣｈｉｎａ ,ＲＥＥｓａｓｆｅｒｔｉｌｉｚｅｒａｄｄｉｔｉｖｅｓｉｎａｇｒｉｃｕｌ ｔｕｒｅｈａｖｅｂｅｅｎｅｘｔｅｎｓｉｖｅｌｙｕｓｅｄ .ＮｕｍｅｒｏｕｓｆｉｅｌｄｅｘｐｅｒｉｍｅｎｔｓａｎｄｐｏｔｓｔｕｄｉｅｓｓｕｇｇｅｓｔｔｈａｔＲＥＥｆｅｒｔｉｌｉｚｅｒａｐｐｌｉｃａｔｉ…     

Influence of Lanthanum on Phosphorus Uptake and Its Chemical Fractions in Rice Crop （Oryza Sativa）

Plantgrowthisrelatednotonlytothetotalphos phoruscontentinplantbutalsotoitschemicalfrac tions .Someresearchersbelievedthatdifferentchemi calfractionsplayeddifferentrolesinplantgrowth ,andthatplantwithlowerinorganic Pcontenthashigherphosphorusmetabolicpotential[1] .Wang[2 ] foundrareearthelements nitrateincreasesphosphorusuptakebywheat (TritcumaestvicumL .)inHoaglandnutrientsolutionculture .Wahidetal .[3] showedthatrareearthelementsappliedinsoildecreasesphosphoruscontentofcoconutseedlings .Dia…     

Effect of Lanthanum on Rice Growth and Physiological Parameters with Split-Root Nutrient Solution Culture

Ａｌｏｔｏｆｗｏｒｋｈａｓｂｅｅｎｄｏｎｅｏｎｔｈｅｅｆｆｅｃｔｏｆｒａｒｅｅａｒｔｈｅｌｅｍｅｎｔｓｏｎｐｌａｎｔｇｒｏｗｔｈａｎｄｐｈｙｓｉｏｌｏｇｉｃａｌａｃｔｉｖ ｉｔｉｅｓ .ＲｅｓｕｌｔｓｈａｖｅｓｈｏｗｎｔｈａｔＲＥＥｓａｐｐｌｉｅｄｂｙｆｏｌｉａｒｓｐｒａｙｓ ,ｓｅｅｄｔｒｅａｔｍｅｎｔｓｏｒａｄｄｉｔｉｏｎｔｏｔｈｅｓｏｉｌｓａｎｄｓｏ ｌｕｔｉｏｎｍｅｄｉａｃａｎｉｎｃｒｅａｓｅｇｒａｉｎｃｒｏｐｓ ,ｅｃｏｎｏｍｉｃｃｒｏｐｓ ,ａｎｄｖｅｇｅｔａｂｌｅｓｂｙ 5 0 %～ 12 % ,8 0 %…     

Toxic Effects of Lanthanum, Cerium, Chromium and Zinc on Potamogeton Malaianus

iththerapiddevelopmentofrareearthindustryandextensiveapplicationofrareearthinagriculture ,agreatmanyofrareearthelements (REEs)werere leasedintoenvironment ,especiallywaterenviron ment ,andtheconsequentseriousenvironmentpollu tionhasattractedpeople′satten…     

Lanthanum Relieved Toxic Effects Caused by Nickel in Hydrocharis dubia B L. Backer Leaves

Rareearthelementshaveextensivelybiologicaleffectsonplants ,animalsandhumanbeingsinthebiosphere[1,2 ] .Researchershavefoundthatrareearthelementscouldpromotetheresistanceofplantagainstsalt[3] ,inundation[4 ] ,aridity[5] andacidrain[6 ] etal .However ,fewreportswereontheirabilityofrelievingthestressofheavymetaltotheplant .Lanthanum(La) ,asanimportantrareearthelement ,alwayspar ticipatesinorinterfereswiththeprocessofbiologicalreactions[7] .ThispapermainlydealtwiththeeffectsofLaonHydrocharisdubia…     

Triton X-100 as a specific inhibitor of the mammalian NADH-ubiquinone oxidoreductase (Complex I)

Triton X-100 inhibits the NADH oxidase and rotenone-sensitive NADH-Q1 reductase activities of bovine heart submitochondrial particles (SMP) with an apparent Ki of 1x10-5 M (pH 8.0, 25 degrees C). The NADH-hexammineruthenium reductase, succinate oxidase, and the respiratory control ratio with succinate as the substrate in tightly coupled SMP are not affected at the inhibitor concentrations below 0.15 mM. The succinate-supported aerobic reverse electron transfer is less sensitive to the inhibitor (Ki=5x10-5 M) than NADH oxidase. Similar to rotenone, limited concentrations of Triton X-100 increase the steady-state level of NAD+ reduction when the nucleotide is added to tightly coupled SMP oxidizing succinate aerobically. Also similar to rotenone, Triton X-100 partially protects Complex I against the thermally induced deactivation and partially activates the thermally deactivated enzyme. The rate of the NADH oxidase inhibition by rotenone is drastically decreased in the presence of Triton X-100 which indicates a competition between these two inhibitors for a common specific binding site. In contrast to rotenone, the inhibitory effect of Triton X-100 is instantly reversed upon dilution of the reaction mixture. The NADH-Q1 reductase activity of SMP is inhibited non-competitively by added Q1 whereas a simple competition between Q1 and the inhibitor is seen for isolated Complex I. The results obtained show that Triton X-100 is a specific inhibitor of the ubiquinone reduction by Complex I and are in accord with our previous findings which suggest that different reaction pathways operate in the forward and reverse electron transfer at this segment of the mammalian respiratory chain.     

Toxicity of Lanthanum to Pathogenic Fungi and Its Morphological Characteristics

Rare earth (RE) ,a kind of natural mineral hasbeen used as a beneficial element to crops for quite alongti me . Withthe solution of a series of keytechnolo-gies such as extraction,separation of radioactive ingre-dients ,sanitary toxicology,and environmental safety,China became the first countryto apply commercial REproducts asfertilizer or growthsti mulatorin agriculture ,which produced considerable economic and social ben-efits[1 ,2].RE has been found to have some controllingeffects on many…     

Preparation and Properties of La^3＋ -Loaded Fluorion Adsorbent

ResearchonF- containingwastewatertreatmenthasbeenpaidgreatattentionbecauseitcanseriouslyharmbodyiffluorioncontentexceedspermittedlimit .Methodstotreatfluorion containingwastewaterarebasedon :adsorption ,reverseosmosis ,ionexchangeresin ,chemicalprecipitation ,coagulatingprecipita tion[1] ,etc .Amongthesemethods ,adsorptionismostwidelyusedtoremovefluorionforitshighefficiency ,easypreparingprocess ,cheaprawmaterial ,andothermerits .Duringmanydefluoridationmaterials ,rareearthcompoundsattackmore…     

Mental retardation, epilepsy, short stature, and skeletal dysplasia: confirmation of the Gurrieri syndrome

AIM: To study the relationship between the calcium and the release of platelet-activating factor (PAF) from rat peritoneal macrophages (PM?) stimulated by lipopolysaccharides (LPS). METHODS: The effects of tetrandrine (Tet), Fura 2-AM, and Bay k 8644 on the PAF release from rat PM? was investigated by the bio-assay of PAF. RESULTS: LPS stimulated PM? to release PAF, without increasing intracellular Ca2+ of PM?, Tet at 0.1, 1.0, 10, 100 mumol.L-1 and Fura 2-AM at 0.01, 0.1, 1.0, 10 mumol.L-1 could dose-dependently decrease the release of PAF (9.8 +/- 1.2, 6.5 +/- 1.6, 4.7 +/- 0.8, 3.4 +/- 0.4 microgram.L-1 and 9.2 +/- 1.7, 5.2 +/- 1.3, 3.7 +/- 0.4, 3.2 +/- 0.3 microgram.L-1, respectively, no drugs 11.8 +/- 1.2 micrograms.L-1), Bay k 8644 at 1.0, 5.0, 10 mumol.L-1 could dose-dependently increase the release of PAF (13.2 +/- 1.7, 16.2 +/- 1.4, 17.6 +/- 1.5 micrograms.L-1), and the effects of Tet and Fura 2-AM were completely or partly reversed by Bay k 8644 at 5.0 mumol.L-1. CONCLUSION: Although LPS did not increase intracellular Ca2+ of PM?, intracellular Ca2+ was necessary for PAF release from rat PM? stimulated by LPS.     

Weaning-induced development of delta-opioid receptors in rat brain: differential effects of guanine nucleotides and sodium upon ligand-receptor recognition

Cibacron Blue F3GA (CB) inhibited the activities of wheat leaves NADH:nitrate reductase and NADH:cytochrome-c reductase in a time-independent and concentration dependent manner. The methyl viologen:nitrate reductase activity of the enzyme was unaffected by various CB concentrations used in the experiment. Inhibition of NADH:nitrate reductase was of mixed type (partial competitive and pure noncompetitive) with respect to NADH and noncompetitive with respect to nitrate. The estimated inhibition constant (Ki) values were 1 microM for NADH and 8.4 microM for nitrate. The secondary plots of inhibition with respect to NADH, indicated a dissociation constant (KI) of 8.8 microM for the enzyme-NADH-CB complex. This KI being greater than the Ki suggested that the noncompetitive inhibition is predominant over the competitive inhibition at the NADH binding site.     

Effects of Neodymium on Nitrogen Metabolism of Plantlet Loquat in Vitro

RareEarthElements (REEs)havespecialelec tricstructuresandtheeffectsonvarietiesofbiologicalactivities .REEspromotingnitrogenmetabolismhasbeenmuchreported[1～4 ] .Ourpreviouspapersstudiedlanthanumandeurioumontherootingofloquatplant letinvitro ,showingthatLaandEucanstimulatethegrowthanddevelopmentofroot ,andenhancetheac tivitiesofnitratereductase (NR)significantly[5,6 ] .ltiswellknownthatnitratereductasecatalyzesthere ductionofNO3- toNH4 .Glutaminesynthetase (GS)andglutamatedehydrogenase…     

镧对绿菊花的生理影响

通过实验室水培试验 ,研究了镧对水生植物绿菊花的某些生理指标如叶绿素总量、抗坏血酸活性酶、过氧化氢酶、过氧化物酶等的影响 ,以及绿菊花对镧的吸收积累作用。结果显示 :短时间内 ,镧对绿菊花生理活性有一定的刺激作用。但长时间 (>7天 )以后高浓度的稀土元素对其生理活性有明显的抑制、破坏作用 ,而低浓度表现为促进—抑制—促进的过程。绿菊花对镧有吸收积累能力     

1，2-双(1′-苯基-3′-甲基-5′-氧代吡唑-4′-基)乙二酮-[1，2]与三正辛基氧化膦协同萃取RE(Ⅲ)的性能和机理研究

研究了1，2-双(1′-苯基-3′-甲基-5′-氧代吡唑-4′-基)乙二酮-[1，2](H     

Toxicity of Lanthanum Against Rhizoctonia solani and Its Effect on Disease-Related Enzymes

Thoroughexperimentsandpracticeaboutrare earth(RE)elementshavebeenconductedsince 1970.ScientistsinChinahavesolvedaseriesofkey technologiesandappliedREelementssuccessfullyin agricultureproduction,andmadeChinabecomethe firstcountryapplyingREasacommercialprod…     

镧对黑曲霉生理代谢的Hormesis效应

应用含有梯度外源镧(La)(0、16mg/L、64mg/L、256mg/L、512mg/L和1024 mg/L)的液体培养基培养黑曲霉,每间隔8h取样测定黑曲霉对还原糖和蛋白质的吸收和利用率。培养3天后,测定黑曲霉质膜三磷酸腺苷酶(ATPase),胞内乳酸脱氢酶(LDH)和淀粉酶活性,以及脂质过氧化产物(MDA)的变化。结果表明,在0～64mg/L外源La作用下,黑曲霉对还原糖和蛋白质的吸收与利用率、质膜Na+,K+-ATPase、Ca2+-ATPase、Mg2+-AT-Pase、胞内LDH酶和淀粉酶活性均随La3+的增加而升高,而MDA呈现下降趋势。在64mg/L～1024mg/L剂量范围内,上述生理指标则呈现相反的变化趋势。因此,La3+诱导了黑曲霉生理代谢的Hormesis效应。     

Instrumental and metabolic evaluation of patients affected by peripheral arterial occlusive disease (PAOD) following surgical revascularization surgery

Experiments were performed on eight subjects affected by peripheral arterial occlusive disease (PAOD) of the lower limbs. Each patient was submitted to Ecodoppler, angiography and the "Treadmill test". Two bioptic muscle of these patients. A sample was used for the spectrophotometric and spectrophotofluorimetric determinations of: glycogen, pyruvate, lactate, citrate, alpha-ketoglutarate, malate, aspartate, glutamate, AMP, ADP, ATP and creatine phosphate (CP). The other bioptic sample was used to determine the following enzyme activities: hexokinase, phosphofructokinase, pyruvate kinase, lactate dehydrogenase, citrate synthase, succinate dehydrogenase, malate dehydrogenase, total NADH cytochrome c reductase, cytochrome oxidase, aspartate aminotransferase and alanine aminotransferase. Patients showed an increase in lactate dehydrogenase, total NADH cytochrome c reductase and succinate dehydrogenase activities, a decrease in glycogen, ATP and CP concentrations. Telethermographic data showed patient muscle thermic emission quantitatively different from control group. The telethermographic test can be used as an additional diagnostic tool to determine and monitor the efficiency of a muscle undergoing metabolic failure.     

Effects of Lanthanum Chloride on Activity of Redox System in Plasma Membrane of Rice Seedling Roots

ａｒｅｅａｒｔｈｓｈａｖｅａｂｅｎｅｆｉｃｉａｌｅｆｆｅｃｔｓｏｎｐｒｏｍｏｔｉｎｇｔｈｅｃｒｏｐｇｒｏｗｔｈａｎｄｉｎｃｒｅａｓｉｎｇｉｔｓｙｉｅｌｄ .Ｓｏｍｅｒｅｓｅａｒｃｈｅｒｓｈａｖｅｄｅｍｏｎｓｔｒａｔｅｄｔｈａｔｔｈｅｒａｒｅｅａｒｔｈｓｃａｎｎｏｔｅｎｔｅｒｔｈｅｐｒｏｔｏｐｌａｓｔａｎｄｏｎｌｙｓｔａｙｏｕｔｓｉｄｅｔｈｅｐｌａｓｍａｍｅｍｂｒａｎｅ[1] .Ｔｈｅｒｅｉｓａｎｏｘｉｄａｔｉｏｎ ｒｅｄｕｃｔｉｏｎ (ｒｅｄｏｘ)ｓｙｓｔｅｍｏｎｔｈｅｐｌａｓｍａｍｅｍｂｒａｎｅｏｆｐｌ…     

Effect of Ln3＋ on Inhibition of Tobacco RuBPcase

Ｉｔｗａｓｐｒｏｖｅｄｂｙｐｒｅｖｉｏｕｓｗｏｒｋｓ[1] ｔｈａｔｒａｒｅｅａｒｔｈｅｌｅｍｅｎｔｓｐｒｏｍｏｔｅｔｈｅｇｒｏｗｔｈａｎｄｄｅ ｖｅｌｏｐｍｅｎｔｏｆｐｌａｎｔｓａｎｄｉｎｃｒｅａｓｅｔｈｅｙｉｅｌｄｏｆｃｒｏｐｓａｔｌｏｗｃｏｎｃｅｎｔｒａｔｉｏｎ ,ｂｕｔｗｈｅｎｔｈｅｃｏｎ ｃｅｎｔｒａｔｉｏｎｉｓｈｉｇｈ ,ｔｈｅｙｗｉｌｌｉｎｈｉｂｉｔｔｈｅｇｒｏｗｔｈｏｆｐｌａｎｔｓ .Ｉｔｗａｓａｌｓｏｐｒｏｖｅｄ[2 ,3] ｔｈａｔｒａｒｅｅａｒｔｈｅｌｅｍｅｎｔｓｅｘｈｉｂｉｔｂｏｔｈｐｏｓ…     

镧对太湖地区水稻土若干水解酶活性的影响

通过模拟试验研究了镧对太湖地区水稻土酸性磷酸酶、脲酶及蔗糖酶活性的影响。镧对水稻土酸性磷酸酶活性表现出抑制作用 ,当镧浓度大于 6 0 mg/kg时 ,抑制作用达到显著水平 ,最大抑制率达到 13%;镧对水稻土脲酶活性影响不显著 ;镧对水稻土蔗糖酶活性表现出一定的刺激作用 ,当镧浓度为 30 0 mg/kg时 ,刺激作用达到显著水平 ,最大刺激率为 10 %。