发酵型鸡肉调味汁的研制

利用干酪乳杆菌发酵鸡肉酶解液制备发酵型鸡肉调味汁。结果表明，发酵工艺条件为液体种子种龄12h、接种量8％、发酵温度37℃、发酵时间15h，得到的发酵液苦味明显消失、氨基态氮含量增加、鸡肉风味增强。此发酵液经调配得到发酵型鸡肉调味汁。     

米曲霉和鲁氏酵母协同发酵优化合浦珠母贝肉酶解液风味

为改善合浦珠母贝肉酶解液风味,采用米曲霉和鲁氏酵母对合浦珠母贝肉酶解液进行协同发酵,并采用正交试验法对其工艺条件进行优化。以发酵液风味值及游离氨基态氮含量为指标,研究菌种比、接种量、发酵温度和发酵时间对合浦珠母贝肉发酵液风味改善的影响。结果表明,米曲霉和鲁氏酵母协同发酵工艺的最佳条件为:米曲霉∶鲁氏酵母=1∶2、接种量4%、发酵温度32℃、发酵时间80 h,该条件下,所得合浦珠母贝肉酶解发酵液的风味值评分与游离氨基态含量分别为96. 77与2. 83 g/L,分别比发酵前提高了59. 40%与26. 34%,贝肉酶解液风味得到显著改善。该研究结果对以合浦珠母贝肉为原料开发海鲜调味料提供了理论基础。     

酵母菌发酵柑橘皮渣生产柠檬酸

以柑橘皮渣为原料,用酵母菌发酵生产柠檬酸,发酵液成分采用纸层层析进行鉴定,发酵液经过钙盐法处理获得柠檬酸固体。通过改变发酵温度、发酵时间、发酵液中甲醇添加量和发酵液中菌种添加量研究发酵最佳条件,根据L9（34）正交试验,最终确定酵母菌发酵柑橘皮渣生产柠檬酸的最佳条件为：发酵温度29℃,发酵时间108h,发酵液中添加8％的酵母活化液和2％的甲醇。     

基于鲶鱼头/鱼排制备美拉德反应物的研究

为提高革胡子鲶鱼(Clarias gariepinus)副产物的附加值,以鲶鱼头/鱼排为基料进行美拉德反应,制备鱼味调味料.将鲶鱼头/鱼排经过高压浸提、酶解、发酵等过程,分别得到酶解液(enzymatic hydrolysate,EH)、发酵液(fermentation broth,FB)、基于酶解的发酵液(ferme...     

乳酸菌发酵猪骨粉中游离钙的研究

本实验利用保加利亚乳杆菌和嗜热链球菌混合菌种对猪骨粉进行发酵,以期提高发酵液中游离钙的含量。通过单因素试验和正交试验,确定发酵猪骨粉的最佳工艺条件为：保加利亚乳杆菌:嗜热链球菌为菌浓度之比2:1,蔗糖添加量为6%、乳酸菌接种量为7%、骨粉添加量为6%,此时发酵液中游离钙的含量为0.9942%,钙磷比为2.3:1。利用乳酸菌发酵猪骨粉,能显著提高发酵液中游离钙的含量。     

“苹果C打”发酵果汁饮料生产工艺研究

以浓缩苹果汁为原料,添加多种乳酸菌发酵,制得发酵液,考察固形物含量、菌种、温度对发酵液的影响,最终确定了最佳发酵条件:浓缩苹果汁固形物含量25°BX,发酵温度42℃,发酵时间72h。再对发酵液进行二次调配,得到营养丰富,风味俱佳的含气发酵苹果汁饮料。     

猪骨素发酵及其产物的电泳分析

对红曲霉发酵猪骨素的条件进行了优化,研究了发酵过程中蛋白酶活力和水解度的变化,可溶性氮、肽基氮、游离氨态氮的变化规律以及产物的性质变化。结果表明,红曲发酵猪骨素的适宜工艺为:猪骨素浓度3%,温度30℃,摇床速度120 r/min,自然pH(6.7～7.0);酶活力和水解度在第5天达到最大,分别为35.92 U/mL和38.66%;游离氨态氮含量在发酵过程中逐渐增加,5 d之后趋于稳定,肽基氮含量在第5天时达到最大值为74.21%;可溶性氮含量在生长初期稍有下降,到第5天时其含量达到最大值为88.45%。Tricine-SDS-PAGE电泳结果显示,发酵过程中蛋白分子质量逐渐降低,发酵6 d,发酵液多肽分子质量最小为8.1 ku。     

糯米酒制作凝固型酸奶的工艺研究

试验利用糯米酒制作凝固型酸奶,米酒发酵液的最适灭菌条件为95℃、5min,原料奶总固体物最佳含量16%.对蔗糖添加量、米酒发酵液添加量、发酵温度、发酵时间和发酵剂添加量做单因素试验,以米酒酸奶的感官评价为指标,通过正交试验,得出凝固型米酒酸奶最佳工艺组合蔗糖添加量7%、米酒发酵液添加量18%、发酵剂添加量1%、发酵温度42℃、发酵时间4h.其中米酒发酵液添加量对米酒酸奶品质影响显著.     

鸡油菌孢子发酵型饮料的研究

主要探讨了以鸡油菌为原料,利用鸡油菌孢子液体深层发酵,获得菌丝体与发酵液的混合液。通过单因素试验及多因素多水平的正交试验确定出饮料的最佳配方,制作鸡油菌孢子发酵型饮料。对鸡油菌孢子发酵型饮料进行了感官质量的评定,结果表明:发酵120h时发酵液的感官最好,多糖含量最高,发酵饮料的因素影响主次顺序为发酵液>稳定剂>柠檬酸>白砂糖,发酵饮料的配方以46%的发酵液、0.22%的柠檬酸、0.18%的稳定剂(CMC-Na)、7.5%的白砂糖时制得的发酵饮料品质最佳。     

双亲灭活的原生质体融合株啤酒酵母DR9-2的构建及其特性的研究

啤酒酵母菌株JW1-1发酵液中的双乙酰、总高级醇的含量较低,但发酵度较低,发酵液中的乙醛含量较高;啤酒酵母菌株NW7-45发酵度较高,发酵液中的乙醛含量较低,但发酵液中的双乙酰、总高级醇的含量较高.为了得到优良的菌株,以紫外线灭活的菌株JW1-1原生质体和热灭活的菌株NW7-45原生质体为亲本进行融合.经三角瓶发酵筛选,得到较优良的融合株DR9-2和DR9-24.其中融合株DR9-2以12°Bx麦芽汁为培养基,用500L的发酵罐在12℃下发酵,发酵11d发酵液的发酵度为69.5%,发酵液中的双乙酰、乙醛和总高级醇的含量分别为0.0124mg/L、7.70mg/L和61.88mg/L.从融合株DR9-2的主要发酵特性和啤酒口感品评的结果表明,该菌株在啤酒酿造工业中具有应用前景.     

Reliable identification of grapevine rootstock varieties using RAPD PCR on woody samples

Since grapevine ( Vitis spp .) rootstock material is being traded increasingly as disbudded woody material a lack of distinctive morphological features on such material necessitates an alternative and reliable means of identification. Methods described here were developed for rapid and efficient extraction of DNA from woody samples rich in phenolic compounds and polysaccharides, and for subsequent identification of varieties by RAPD PCR. Using these methods, and with the application of only one selected RAPD primer, we were able to differentiate sixteen rootstock varieties, including the seven varieties most commonly used in Germany. Problems commonly encountered with reproducibility of RAPD patterns were avoided by choosing primers with a dinucleotide sequence and a high G/C content that allowed a rather high annealing temperature of 45°C. Methods described here should also be useful for other horticultural crops, especially those with woody tissues rich in phenolic compounds and polysaccharides.     

An internet compendium of analytical methods and spectroscopic information for monomers and additives used in food packaging plastics

An internet website (http://cpf.jrc.it/smt/) has been produced as a means of dissemination of methods of analysis and supporting spectroscopic information on monomers and additives used for food contact materials (principally packaging). The site which is aimed primarily at assisting food control laboratories in the European Union contains analytical information on monomers, starting substances and additives used in the manufacture of plastics materials. A searchable index is provided giving PM and CAS numbers for each of 255 substances. For each substance a data sheet gives regulatory information, chemical structures, physico-chemical information and background information on the use of the substance in particular plastics, and the food packaging applications. For monomers and starting substances (155 compounds) the infra-red and mass spectra are provided, and for additives (100 compounds); additionally proton NMR are available for about 50% of the entries. Where analytical methods have been developed for determining these substances as residual amounts in plastics or as trace amounts in food simulants these methods are also on the website. All information is provided in portable document file (PDF) format which means that high quality copies can be readily printed, using freely available Adobe Acrobat Reader software. The website will in future be maintained and up-dated by the European Commission's Joint Research Centre (JRC) as new substances are authorized for use by the European Commission (DG-ENTR formerly DGIII). Where analytical laboratories (food control or other) require reference substances these can be obtained free-ofcharge from a reference collection housed at the JRC and maintained in conjunction with this website compendium.     

Aroma characterization of various apricot varieties using headspace–solid phase microextraction combined with gas chromatography–mass spectrometry and gas chromatography–olfactometry

The characterization of the aromatic profile of several apricot cultivars with molecular tracers in order to obtain objective data concerning the aromatic quality of this fruit was undertaken using headspace–solid phase microextraction (HS–SPME). Six apricot cultivars were selected according to their organoleptic characteristics: Iranien, Orangered, Goldrich, Hargrand, Rouge du Roussillon and A4025. The aromatic intensity of these varieties measured by HS–SPME–Olfactometry were defined and classified according to the presence and the intensity of grassy, fruity and apricot like notes. In the six varieties, 23 common volatile compounds were identified by HS–SPME–GC–MS. Finally, 10 compounds, ethyl acetate, hexyl acetate, limonene, β-cyclocitral, γ-decalactone, 6-methyl-5-hepten-2-one, linalool, β-ionone, menthone and (E)-hexen-2-al were recognized by HS–SPME–GC–O as responsible of the aromatic notes involved in apricot aroma and considered as molecular tracers of apricot aromatic quality which could be utilized to discriminate apricot varieties.     

Tests of potential functional barriers for laminated multilayer food packages. Part I: Low molecular weight permeants

The advent of the functional barrier concept in food packaging has brought with it a requirement for fast tests of permeation through potential barrier materials. In such tests it would be convenient for both foodstuffs and materials below the functional barrier (sub-barrier materials) to be represented by standard simulants. By means of inverse gas chromatography, liquid paraffin spiked with appropriate permeants was considered as a potential simulant of sub-barrier materials based on polypropylene (PP) or similar polyolefins. Experiments were performed to characterize the kinetics of the permeation of low molecular weight model permeants (octene, toluene and isopropanol) from liquid paraffin, through a surrogate potential functional barrier (25 μm-thick oriented PP) into the food simulants olive oil and 3% (w/v) acetic acid. These permeation results were interpreted in terms of three permeation kinetic models regarding the solubility of a particular model permeant in the post-barrier medium (i.e. the food simulant). The results obtained justify the development and evaluation of liquid sub-barrier simulants that would allow flexible yet rigorous testing of new laminated multilayer packaging materials.     

Analysis of benzo[a]pyrene in spiked fatty foods by second derivative synchronous spectrofluorimetry after microwave-assisted treatment of samples

A simple, rapid and inexpensive method has been developed for the determination of benzo[a     

The development of reference materials for paralytic shellfish poisoning toxins in lyophilized mussel. II: Certification study

This paper describes the second part of a project undertaken to develop certified mussel reference materials for paralytic shellfish poisoning toxins. In the first part two interlaboratory studies were undertaken to investigate the performance of the analytical methodology for several PSP toxins, in particular saxitoxin and decarbamoyl-saxitoxin in lyophilized mussels, and to set criteria for the acceptance of results to be applied during the certification exercise. Fifteen laboratories participated in this certification study and were asked to measure saxitoxin and decarbamoyl-saxitoxin in rehydrated lyophilized mussel material and in a saxitoxin-enriched mussel material. The participants were allowed to use a method of their choice but with an extraction procedure to be strictly followed. The study included extra experiments to verify the detection limits for both saxitoxin and decarbamoyl-saxitoxin. Most participants (13 of 15) were able to meet all the criteria set for the certification study. Results for saxitoxin.2HCl yielded a certified mass fraction of <0.07 mg/kg in the rehydrated lyophilized mussels. Results obtained for decarbamoyl-saxitoxin.2HCl yielded a certified mass fraction of 1.59+/-0.20 mg/kg. The results for saxitoxin.2HCl in enriched blank mussel yielded a certified mass fraction of 0.48 +/- 0.06 mg/kg. These certified reference materials for paralytic shellfish poisoning toxins in lyophilized mussel material are the first available for laboratories to test their method for accuracy and performance.     

Announcing a new international peer-reviewed journal:Food Science and Human Wellness now accepting manuscript submission in English

<正>We are pleased to announce the launch of a new international peer-reviewed journal-Food Science and Human Wellness,ISSN 2213-4530,which is an open access journal,produced and hosted by Elsevier B.V.on behalf of Beijing Academy of Food Sciences.Food Science and Human Wellness is an international peer-reviewed English journal that provides a forum for the dissemination of the     

Chinese CTP Market

According to Printing and Printing Equipment Industries Association of China（PEIAC）＇s statistics to the plate manufucturer in China, in 2013, the actual offset plate production has reached 346 million square meters in China. Among them, the CTP production volume was 245 million square meters, up by 11% than that of last year; the total sales of the CTP plate was 239 million square meters, up by 13%.     

Preparatory Work of Print China 2015 is Going Smoothly

正Held at Guangdong Modern International Exhibition Center,Print China 2015 will cover 7exhibition halls,besides the original Hall No.3,4,5,6,7,the newly built F zone of Hall 3 will be used too.The total area will be140,000 square meters.Hall 3:Offset and large printing equipment,package printing equipment,post press