Viscoelasticity, texture and ultrastructure of cut apple as affected by sequential anti-browning and ultraviolet-C light treatments

The aim of this work was to analyze the effect of UV-C radiation (fluence: 11.2 kJ/m²), with or without an anti-browning pretreatment containing 1% (w/v) ascorbic acid plus 0.1% (w/v) calcium chloride, on the linear viscoelastic properties (oscillatory shear and creep/recovery), instrumental texture (TPA), sensory texture and ultrastructure (ESEM, TEM) of cut apple. Changes in structural features and viscoelastic parameters were mainly evidenced after refrigerated storage. All samples showed a viscoelastic solid behavior with the storage modulus (G′) dominating the viscoelastic response. Overall, both dynamic moduli decreased, and instantaneous compliance (J₀), decay compliances (J₁ and J₂) and fluidity significantly increased after treatments and storage at 5 °C, while retardation times were in general constant. Fracture properties proved to be the most highly correlated with sensory texture. The test panel only significantly differentiated stored untreated apple from the other samples regarding fracturability and juiciness. Mechanical spectra and creep parameters showed ability to evidence ultrastructural differences (rupture of membranes, swelling of cells, alteration of cell walls) in the surface of cut apples subjected to the different treatments.     

Prediction of the rheological properties of wheat dough by starch-gluten model dough systems: effect of gluten fraction and starch variety

The present study sought to investigate the rheological properties of wheat starch-gluten (WS-G) and potato starch-gluten (PS-G) model doughs with different gluten fractions to elucidate the effectiveness of using model dough to predict wheat dough properties. The highest linear viscoelastic region, frequency dependence, maximum creep compliance and the lowest viscoelastic modulus and zero shear viscosity were observed in the wheat dough, followed by WS-G and PS-G model doughs. PS exerted a more significant damage effect on the gluten network while WS shared a tight integration with gluten protein, forming a more stable dough structure. The viscoelasticity of the model doughs shared a close association with the wheat dough under increased gluten fraction, while the frequency dependence of the model doughs showed no trend towards wheat dough. Therefore, starch-gluten model dough could not fully stimulate the functionality of wheat dough irrespective of its gluten fraction.     

稻米-高筋小麦混合粉面团的静态和动态流变学特性

本实验研究了6 种稻米-高筋混合粉面团的静态和动态流变学特性。结果表明,随着稻米粉添加量的增加,在动态流变频率扫描中,混合面团的储存模量（G’）和损耗模量（G’’）整体呈上升趋势,损耗角正切（tan δ）整体呈规律性下降趋势;在静态流变的蠕变松弛实验中,最大蠕变应变量、最大蠕变柔量和瞬时恢复柔量逐渐减小,零剪切黏度和瞬间恢复比率逐渐增加,表明加入稻米粉后面筋蛋白有所稀释,但稻米粉中的淀粉吸水膨胀相互黏附,且与米蛋白相互作用赋予面团更高的弹性模量和更复杂的内部结构。在动态流变的温度扫描中,糊化温度前后混合面团的黏弹性变化明显,G’和G’’曲线类似淀粉糊化曲线,说明生面团加热的过程中,影响其流变学特性的主要成分为淀粉;降温过程中,稻米粉添加量的影响较小,高温区G’和G’’呈规律性增加,温度低于60 ℃后各项流变指标规律性差;在整个降温的过程中样品均具有类固体的性质。     

FLOW CURVES, STRESS RELAXATION AND CREEP MEASUREMENTS OF STARCH GELS

Flow curves, stress relaxation, and creep compliance were measured for various types of corn starch gel that underwent different degrees of modification. The four starches were native corn starch, medium hydroxypropylated distarch phosphate, highly hydroxypropylated distarch phosphate, and distarch phosphate. All the starches exhibited pseudo-yield stress behavior at low shear rates and shear-thinning at higher shear rates. Stress relaxation data at different strains indicated strain-softening phenomenon for all starches. Higher degrees of cross-linking decreased the half relaxation time due to the addition of rigid filler into the matrix. Increasing the concentration increased the relaxation modulus for all starches. Creep compliance and recovery measurements were conducted over the range of 3.2 Pa-320 Pa. In the linear viscoelastic region, the compliance data were not dependent on the magnitude of stress applied. As concentration increases, higher stress magnitude leads to nonlinear behavior. The creep compliance data were fitted into the Ninomiya and Ferry relation from dynamic viscoelastic data and the Maxwell-Voigt model. Ninomiya and Ferry overpredicted the relaxation data while the Maxwell-Voigt model fitted the data reasonably well.     

抗性淀粉与面粉共混体系粘弹性的研究

采用微波湿热-循环冷冻制备小麦抗性淀粉粗品,以酶解纯化后得到的抗性淀粉纯品添加小麦粉中,研究其在不同替代比例（5%,10%,15%和20%）条件下对面团流变特性的影响。研究表明：不同比例的抗性淀粉与面粉共混体系其表观黏度均随着剪切速率的增大而减小,呈现出“剪切稀化”现象,表现出假塑性流体特性;随着替代比例的增加,面团的储能模量以及损失模量也显著升高,损耗角正切值tanδ逐渐降低,面团弹性增加。与对照组相比,试验组替代比为5%和10%时,面团的最大蠕变柔量(Jmax)、瞬时蠕变柔量（J0)和迟滞蠕变柔量(J1)呈现出明显降低的趋势,继续增大替代比,变化不显著。研究结果表明小麦粉中添加抗性淀粉显著改变了共混体系的粘弹性,使面团的硬度随着替代比的增加而增大。     

Storage time effect on the rheology of refrigerated potato tissue (cv. Monalisa)

 Potato tubers were held in refrigerated storage and their firmness periodically sampled over a period of 140 days. At each sampling, the rheological properties uniaxial compression, shear, uniaxial tension, successive cycles of stress relaxation, creep compliance and texture profile analysis were determined, as was moisture content. Compression energy, maximum shear force, longitudinal tension stiffness and maximum tension deformation, stress relaxation parameters and moisture content were all significantly affected by storage time. Relaxed forces decreased consistently and linearly with time over a 140-day storage period, reflecting the decrease in cell turgor pressure resulting from the predominance of water loss through evaporation over water production. Longitudinal tension stiffness and maximum shear force increased linearly in the early stages, reflecting the increase in cell wall stiffness and elastic tissue behavior. Maximum tension deformation increased linearly with time reflecting the plastic deformation of the cell wall caused by growth in cell turgor pressure at early stages. Changes in the trends of rheological parameters over 140, 64 and 28 days evidenced the importance of extending the time period considered for rheological parameters of potato tissue. Received: 28 December 1999     

Viscoelastic Characterization of Sage Seed Gum

Wild sage seed is a small, rounded, and mucilaginous seed, which comes from Salvia macrosiphon. The viscoelastic behavior of sage seed gum, at different concentrations (0.5–2%, w/w), was examined by measuring the transient (in-shear structural recovery and creep/recovery tests) and dynamic (stress and frequency sweeps) rheological properties. The mechanical spectra showed typical weak gel behavior at all concentrations, with storage modulus higher than loss modulus, and little variation with frequency. Both moduli greatly increased with increasing the concentration, and the concentration dependency was well described by the power-law model. The loss tangent was increased slightly with increasing the frequency in the range of 0.25–0.67, although it was not affected by an increase in gum concentration. Moreover, the complex viscosity was found to increase with the increase of sage seed gum concentration and to decrease linearly with the increase of frequency. All samples showed typical viscoelastic response to stress in creep/recovery tests, with recoverable strain increasing in direct proportion to sage seed gum concentration. Creep curves were adequately fitted with a Burger model of four parameters. The elastic and viscous contributions to the general viscoelastic behavior were analyzed through the obtained parameters. The concentration had no specific effect on the in-shear recovery properties of sage seed gum gels, and the gel structure was highly recovered after applying shear. The results of this article indicated that sage seed gum may offer an excellent alternative for commercial gums as a thickening/gelling agent.     

Rheological characterization of fresh and cooked potato tissues (cv.?Monalisa)

 The rheological properties of fresh and cooked (15 min in boiling water) potato tissue which had been deformed to a lessor or greater extent by uniaxial compression, shear, uniaxial tension, successive cycles of stress relaxation, creep compliance and texture profile analysis were evaluated. Structural failure of fresh tissue under compression always occurred along a single plane of maximum shear stress, while fresh tensile specimens failed under tension. Tensile tests proved better methods by which to determine the failure parameters of cooked specimens since it was possible to observe two differents modes of failure with this technique. Equivalent modes of failure (causing damage to the cell wall or cell separation) and changes in structural components caused by cooking proved easier to idetify with tensile tests. The six element Burger's model incorporating two discrete Voigt-Kelvin units was the most suitable for defining tissue creep behaviour. The instantaneous elastic modulus could be related to the internal cell pressure, and gelatinization of starch and viscoelastic units appeared to reflect the viscoelastic properties of pectic sustances and hemicelluloses, respectively. Received: 1 December 1997     

CREEP BEHAVIOUR OF TOMATO PERICARP TISSUE AS INFLUENCED BY AMBIENT TEMPERATURE RIPENING AND CHILLED STORAGE

The influence of normal ripening and chilling stress on viscoelastic properties of tomato pericarp tissue were investigated by measuring creep behaviour of tissue from fruit stored at 22C (nonchilled) or 5C (chilled) for 28 days, or at 5C for 16 days prior to transfer to 22C for an additional 12 days (prechilled). Creep compliance of tissue from all treatments subjected to a constant shear stress of 150 Pa for 5 min was best represented by a 6-element Burgers model containing two discrete Voigt-Kelvin units characterizing fast and slow rate viscoelastic properties. The magnitude of instantaneous elastic, viscoelastic and steady-state viscous compliances each increased steadily and contributed to the overall softening of nonchilled and prechilled tissues during ripening, but remained unchanged during chilling of tomato fruit. Increased fluidity of ripening tissues occurred at the expense of elasticity, consistent with a decrease in molecular weight-size distribution of structural elements contributing to respective viscoelastic properties. The physico-mechanical changes in prechilled tissue preceded those in nonchilled tissue by several days, and occurred at a faster rate. The 6-element Burgers model defining the creep behaviour of tomato pericarp tissue was interpreted with respect to general plant cell wall structure and biochemical changes known to occur during ripening of tomato fruits. Multiple mechanisms of softening were thereby consolidated into a single physico-mechanical model.     

Effects of gelation temperature on Mozzarella-type curd made from buffalo and cows’ milk. 1: Rheology and microstructure

The rheology and microstructure of Mozzarella-type curds made from buffalo and cows’ milk were measured at gelation temperatures of 28, 34 and 39 °C after chymosin addition. The maximum curd strength (G′) was obtained at a gelation temperature of 34 °C in both types of bovine milk. The viscoelasticity (tan δ) of both curds was increased with increasing gelation temperature. The rennet coagulation time was reduced with increase of gelation temperature in both types of milk. Frequency sweep data (0.1–10Hz was recorded 90 min after chymosin addition, and both milk samples showed characteristics of weak viscoelastic gel systems. When both milk samples were subjected to shear stress to break the curd system at constant shear rate, 95 min after chymosin addition, the maximum yield stress was obtained at the gelation temperatures of 34 °C and 28 °C in buffalo and cows’ curd respectively. The cryo-SEM and CLSM techniques were used to observe the microstructure of Mozzarella-type curd. The porosity was measured using image J software. The cryo-SEM and CLSM micrographs showed that minimum porosity was observed at the gelation temperature of 34 °C in both types of milk. Buffalo curd showed minimum porosity at similar gelation temperature when compared to cows’ curd. This may be due to higher protein concentration in buffalo milk.     

Effects of Chemical and Enzymatic Modification on Dough Rheology and Biscuit Characteristics

ABSTRACT: The effect of addition of sodium meta-bisulfite and a commercial protease on dough rheological properties and biscuit characteristics was studied on 7 biscuit wheat cultivars. Sodium meta-bisulfite (SMS) (360 mg/kg flour) or protease (300 mg/kg flour) was added to semisweet biscuit dough. Rheological studies included creep recovery and shear oscillation. SMS and protease increased maximum strain, recovery strain, and phase tan Δ, and lowered storage modulus, G', and the relative recovery, % recovery. The effects varied among cultivars and between SMS and protease. Biscuit eccentricity (width/length) was significantly reduced by addition of SMS or protease, but the effect varied among the cultivars, and between SMS and protease. Partial least squares regression analysis of the rheological parameters and the dimensional characteristics showed that biscuit contraction and spread were mostly correlated to % recovery of the dough, and protein and gluten content of the flour. Correlations between predicted and measured contraction and spread were r = 0.70 and r = 0.43, respectively.     

Study on Resistant Starch Functionality in Short Dough Biscuits by Oscillatory and Creep and Recovery Tests

The effect of wheat flour replacement by a resistant starch-rich ingredient (RSRI) on the structure of short dough biscuits was studied by oscillatory and creep and recovery tests to determine linear viscoelastic properties. The RSRI was substituted for the flour at three different levels, 20%, 40% and 60% (w/w). The use of RSRI increased the elastic and the viscous moduli but did not influence tan δ. The compliance values during the creep test were adjusted successfully to the Burger model. The creep and recovery test revealed an increase in elasticity and resistance to flow and a decrease in deformability with RSRI, but the differences were only significant at the 40% and 60% levels. The RSRI did not affect relative recovery, thus no effect on the type of structure is expected. Deformability was positively correlated with biscuit spread during baking.     

A COMPUTERIZED METHOD TO ANALYZE THE CREEP BEHAVIOR OF VISCOELASTIC FOODS

A computer-aided method was developed to simplify the analysis of creep behavior and to determine the elasticity, the Newtonian viscosity and individual viscosities of viscoelastic foods. The method enabled the inclusion of all creep compliance data in the analysis, and reduced the analysis time from hours to minutes. The method was tested in the calculation of rheological properties of wheat flour dough.     

Reduction of rutin loss in buckwheat noodles and their physicochemical characterisation

Tartary buckwheat was subjected to hydrothermal treatments for minimising rutin loss in buckwheat-based foods by water addition. When native buckwheat flour was mixed with water for 60 min, the rutin content was distinctly reduced from 3.74 g/100 g to 0.31 g/100 g, increasing the amount of quercetin. However, the rutin content remained constant and quercetin was hardly detected in hydrothermally-treated buckwheat flour. Also, when noodles were prepared with wheat and buckwheat flours (7:3, w/w), the noodle samples containing hydrothermally-treated buckwheat flour, showed higher amounts of rutin (more than 0.83 g/100 g) than the control noodle with native buckwheat flour (0.27 g/100 g). In addition, the use of hydrothermally-treated buckwheat flour gave less pasting parameters and lower viscoelastic properties. The noodle dough with hydrothermally-treated buckwheat flour also had greater water absorption and development time during mixing while the elongation stress of the noodle dough was reduced.     

VISCOELASTIC BEHAVIOR OF ARZÚA-ULLOA CHEESE

A study of the viscoelastic properties of ten samples of commercial Arzúa-Ulloa cheeses produced according to the regulations of the designation of origin is reported. The linear viscoelastic range at 20C was determined from stress sweep tests and later confirmed by transient tests. The shear creep compliance curves were analyzed in the framework of the generalized Burgers model. Mechanical spectra at 20C obtained from frequency sweep tests completed the study. The results thus obtained were highly consistent themselves and they manifested the structural differences among the samples. Some correlation between the dry extract content and the viscoelastic behavior was found.     

USE OF THE BIRD-LEIDER EQUATION IN FOOD RHEOLOGY

Shear stress development at inception of a constant shear rate was studied for marshmallow cream, peanut butter, squeeze margarine, tub margarine, whipped butter, whipped cream cheese and whipped dessert topping using the cone and plate geometry of the Rheometrics Mechanical Spectrometer. For most foods studied transient shear stresses showed increasing shear stress oversoots with increasing shear rate. At the highest shear rate of 100 s^?1, transient stresses were as large as 425% of the steady state shear stresses, with the actual magnitude of overshoot depending on the particular food investigated. The Bird-Leider equation was chosen to model this time dependent flow behavior by incorporating both steady viscous and elastic properties of the foods. By assuming that the viscosity function and primary normal stress coefficient followed power-law behavior, and constructing a time constant from the calculated parameters, the Bird-Leider model provided a good prediction of maximum and steady state stresses as well as the time at which they occur, but only a crude prediction of shear stress decay. The model supplied two more empirical constants “a” and “b” where “a” can be regarded as a pseudo-elastic modulus. Variation in “a” with shear rate shows that all materials studied are nonlinear viscoelastic.     

Viscoelastic properties of wheat gliadin and glutenin suspensions

Linear and non-linear rheological properties of wheat gliadin and glutenin suspensions were investigated at various concentrations. Linear dynamic viscoelastic properties for both gliadin and glutenin were strongly dependent on concentration. For gliadins, the storage moduli (G′), loss moduli (G″), and phase shifts dramatically changed within a narrow concentration range, indicating that gliadin suspension properties changed from viscous to viscoelastic. Glutenins exhibited viscoelastic solid behaviour at all measured concentrations. The non-linear shear viscoelastic properties of gliadin and glutenin also depended on concentration. Viscosities of gliadins displayed shear-thinning behaviour; viscosities for glutenins showed shear-thickening behaviour at low shear rates, and shear-thinning behaviour at higher shear rates. Our results indicate that gliadin’s structure in suspension changes over a small concentration range, and suggest that gliadin is important in adjusting and controlling gluten’s viscoelastic behaviour, and not only as a diluent of gluten’s functional properties.     

Creep/Recovery Behavior of Oil-Water Emulsions: Influence of Disperse Phase Concentration

The rheological (creep/compliance) behavior has been studied for a series of soya oil (soybean oil)-water emulsions with the disperse phase (oil) concentration ranging from 20% to 70%. The compliance response was due to three mechanisms: instantaneous elastic, retarded elastic and viscous flow. It was necessary to postulate the presence of two retardation processes to account for the Theological behavior. As the oil concentration was increased, the emulsions became more elastic, with the instantaneous elastic mechanism becoming more dominant. In the non-linear region, the viscous flow was more dominant, with the emulsions becoming less elastic with increasing shear stress. The results are rationalized in terms of the breaking and reforming of bonds in the network like coagulation structure.     

The Interplay Between the Main Flour Constituents in the Rheological Behaviour of Wheat Flour Dough

There is still considerable debate in the literature about the respective roles of starch and gluten in both the linear and non-linear rheology of wheat flour dough. Hence, to elucidate the individual contributions of gluten and starch to the overall dough behaviour, the rheological properties of dough and mixtures of different gluten-starch ratios were studied systematically in shear and extension, by means of an adequate rheological toolbox consisting of linear small amplitude oscillatory shear tests and non-linear tests such as creep-recovery in shear and uniaxial extension. The starch component plays a pivotal role in linear dough rheology. With increasing starch content, the linearity limit observed in oscillatory shear tests decreases as a power-law function. Starch also clearly affects the extensional viscosity at small strains. Consequently, in the linear region differences between different gluten systems may become obscured by the presence of starch. As breadmaking qualities are known to be intrinsically linked to the gluten network, it is imperative to probe the non-linear behaviour of dough in order to expose differences in flour quality. The quality differences between a strong and a weak flour type were revealed most clearly in the value of the strain-hardening index in uniaxial extension and the total recovery compliance in non-linear creep-recovery tests. Notwithstanding its earlier successful application to pure gluten gels, the accuracy of the critical gel model in predicting the linear rheological properties of dough was found to be limited, due to dough having a small linearity limit and a finite longest relaxation time.     

Rheological and baking characteristics of batter and bread prepared from pregelatinised cassava starch and sorghum and modified using microbial transglutaminase

The effect of different concentrations (0, 0.5, 1 and 1.5 U/g) of microbial transglutaminase (MTG) on the creep-recovery properties of gluten-free batter prepared from pregelatinised cassava starch, sorghum and egg white was investigated. The test conducted in the rheometer had an instant loading of 80 Pa for 60 s and recovery of 0 Pa for 140 s. Increasing MTG concentration decreased the batters’ resistance to deformation and compliances but increased zero shear viscosity and elastic recovery. Changes in batter rheological properties were insignificant (P > 0.05) at MTG concentrations beyond 0.5 U/g. Crumb properties of gluten-free bread baked from the batter revealed that increasing MTG concentration increased (P < 0.05) crumb firmness and chewiness, whereas increasing incubation time decreased (P < 0.05) crumb cohesiveness, chewiness and resilience. There were no significant interaction effects (P > 0.05) between enzyme concentration and incubation time.