间歇性添加蒸汽对餐包品质的影响

通过测定餐包升温曲线、质构、丙烯酰胺含量及风味物质等参数,研究了焙烤时蒸汽添加频率(低、中、高频率,对应添加蒸汽的时间间隔分别为4、2、1 min)对餐包品质的影响。结果发现,相比无蒸汽组,蒸汽的加入使餐包中心升温速率总体更加平缓。较之无蒸汽组,中高频率引入蒸汽餐包的烘焙损失率降低(P<0.05)。随着蒸汽频率增高,餐包表皮中心的L*值和b*值上升,而a*值下降,通过质构测定,加入蒸汽使餐包表皮硬度和餐包硬度均显著降低(P<0.05),其耐咀嚼度和回复性随蒸汽频率增高呈先下降后上升的趋势。蒸汽的引入使醇类、壬醛和3-羟基-2-丁酮等物质的相对含量升高,而糠醛的相对含量降低,使面包在具有更加浓厚的香气的同时,减少焦糊味。同时,蒸汽的引入改变了餐包表皮的微观结构,降低了餐包整体及其表皮的丙烯酰胺生成量,其中表皮由350 μg/kg降低为218、141、83 μg/kg(依次为低、中、高频率组),整体由74 μg/kg降低为59、55、41 μg/kg(依次为低、中、高频率组)。餐包表皮赖氨酸的残留量显著增加(P<0.05),由无蒸汽组的0.97 g/kg增加至高频率组的1.58 g/kg。最后结合感官评价得出,当蒸汽加入时间间隔为2 min时,餐包色香适中且营养安全品质较优。     

Influence of the amount of steaming during baking on the kinetic of heating and on selected quality attributes of bread

The effect of the amount of steam injection on selected bread characteristics were investigated using a deck oven (1 m² internal surface). Baking was done at 200 °C for 20 min with steaming of 100, 200, 300, 400 and 500 ml. The temperature at center of the bread and the CO₂ concentration in the oven have been measured during baking. Specific volume, moisture loss and crust crumb ratio were measured after baking.The heating rate between 35 and 55 °C was considered to compare the steaming conditions. For low steaming (100 and 200 ml), the heating rate was significantly higher (p < 0.05) than those at higher steaming (400-500 ml). The heating rate at 300 ml was between the 100-200 and 400-500 ml groups. This difference was attributed to the condensation of steam on the loaf for higher steaming, which in turns slows down the heating rate. The largest bread volume was obtained either for low or high steaming. However, tearing of the crust was observed for low steaming. The crust-crumb ratio was increasing with decreasing amount of steaming. The amount of CO₂ released during baking was higher for the highest amount of steaming; however, this result was not statistically different except between 100 and 500 ml. This could be attributed to a slower heating rate which in turns favors the secondary production of CO₂ during baking until thermal inactivation of CO₂.     

Effect of the amount of steam during baking on bread crust features and water diffusion

Surface mechanical properties in fresh bread and during storage are greatly affected by the water plasticizing effect. However, the incidence of steaming during baking on the crust mechanical properties remains still unclear. The impact of the amount of steam (100, 200 and 400 ml) during baking on the crust features and water diffusivity was investigated. The amount of steam significantly (p < 0.05) affected the crust colour, glossiness and mechanical properties. An increase in the amount of steam led to reduced colour, failure force and failure firmness, whereas increased glossiness. Water vapour transfer rate and water vapour permeability of the bread crust significantly decreased when increasing the amount of steam applied during baking. Crust microstructure studies carried out by scanning electron microscopy and X-ray microtomography confirmed that the amount of steam greatly affected the surface starch gelatinization and also the protein-starch network.     

Influence of Amyloglucosidase in Bread Crust Properties

Enzymes are used in baking as a useful tool for improving the processing behavior or properties of baked products. A number of enzymes have been proposed for improving specific volume, imparting softness, or extend the shelf life of breads, but scarce studies have been focused on bread crust. The aim of this study was to determine the use of amyloglucosidase for modulating the properties of the bread crust and increase its crispness. Increasing levels of enzyme were applied onto the surface of two different partially bake breads (thin and thick crust bread). Amyloglucosidase treatment affected significantly (P?<?0.05) the color of the crust and decreased the moisture content and water activity of the crusts. Mechanical properties were modified by amyloglucosidase, namely increasing levels of enzyme promoted a decrease in the force (Fm) required for crust rupture and an increase in the number of fracture events (N _wr) related to crispy products. Crust microstructure analysis confirmed that enzymatic treatment caused changes in the bread crust structure, leading to a disruption of the structure, by removing the starchy layer that covered the granules and increasing the number of voids, which agree with the texture fragility.     

Shelf-life stability of artisanally and industrially produced durum wheat sourdough bread (“Altamura bread”)

Physico-chemical properties and volatile compounds of three commercial Altamura breads were evaluated during storage at 25 °C. Two protected denomination of origin (PDO) artisanally produced Altamura breads (Bari, Italy), characterized either by high (High A) or low (LowA) loaf, and an industrial product, commercialized as “Altamura like” (IndA), were studied.HighA and LowA breads had a tick crust that was also detached from the crumb creating an air cushion between crust and crumb. IndA products had a thinner crust, a more homogeneous crumb structure as well as a more homogeneous water distribution among the different portion of the bread loaf than HighA and LowA. A more pronounced water gradient characterized the artisanal breads. Crust and under crust portion of all breads, and crumb for IndA product, underwent a significant reduction of moisture content and a_w during storage. Both artisanal breads were subjected to a more significant crumb hardening than IndA sample. Fresh crusts of artisanally produced breads were also significantly harder than IndA. Fresh IndA samples were significantly less cohesive and less springy than artisanal products; cohesiveness significantly decreased in all samples during storage. A more complex gas chromatographic profile was found in the artisanal bread as a larger amount of volatile compounds was present as compared to the IndA bread. Volatile compounds originated both from microbial activity and non-enzymatic browning. Larger amount of volatile compounds characteristics of yeast fermentation was found in IndA. Volatiles decreased over storage in both samples, more significant in the IndA product.     

Water Binding of Wheat Flour Doughs and Breads as Studied by Deuteron Relaxation

Water mobility in wheat flour doughs and breads was investigated by deuteron relaxation using pulsed NMR. Water was replaced by deuterium oxide in dough and bread at different concentrations. Mixograms indicated that wheat flour associated more strongly with D₂O than it did with H₂O. Varying the D₂O:H₂O ratio of flour doughs had no effect on the longitudinal (T₁) or transverse (T₂) relaxation times of deuteron. Hard wheat and soft wheat flour doughs showed similar increases in T₁ and T₂ with increasing moisture content. Staling of bread crumb was accompanied by decreased T₁ and T₂, indicating an overall decrease in water mobility and increase in water binding. The decrease in water mobility of bread crumb with storage time was shown to be independent of reduction in moisture content.     

Biochemical characteristics of Trametes multicolor pyranose oxidase and Aspergillus niger glucose oxidase and implications for their functionality in wheat flour dough

Similar to glucose oxidase (GO), pyranose oxidase (P₂O) may well have desired functionalities in some food applications in general, particularly breadmaking. As its name implies, P₂O oxidises a variety of monosaccharides. P₂O purified from a culture of Trametes multicolor (P₂O-Tm) had high affinity towards d-glucose (K_M = 3.1 mM) and lower affinity to other monosaccharides. GO from Aspergillusniger (GO-An) had a K_M value of 225 mM towards glucose, which points to a significant difference in glucose affinity between the two enzymes. Furthermore, P₂O-Tm had higher affinity towards O₂ (K_M = 0.46 mM) than GO-An (K_M = 2.9 mM). Dehydroascorbic acid did not accept electrons in the reactions catalysed by P₂O-Tm and GO-An. For the same activity towards glucose in saturating conditions, the rate of ferulic acid oxidation in a model system and of thiol oxidation in a wheat flour extract were higher with P₂O-Tm, than with GO-An. The demonstrated differences in properties and functional features between P₂O-Tm and GO-An allow prediction of differences in functional behaviour of the enzymes, in food applications.     

Water dynamics in white bread and starch gels as affected by water and gluten content

Three levels of water (180, 220 and 260 g/345 g flour) and three levels of gluten (giving 11.2, 13.0 and 14.2 g protein/100 g flour) were used to study the effects of water and gluten on water mobility in white bread using ¹H nuclear magnetic resonance (NMR) relaxometry. Changes in the transverse relaxation time (T₂) were related to water mobility. The three water levels resulted in three different moisture contents in the finished bread (0.55-0.77 g H₂O/g solids). Distributed exponential analysis showed two distinct regions of T₂ (30-600 μs and 1-60 ms), associated with multiple domains of water in the bread crumb. There was no significant difference in peak T₂ values with different gluten content, but significant differences were observed with different moisture content. The results suggested that the mobility of water associated with starch decreased dramatically because of gelatinization. To further investigate the effect of gluten on starch gelatinization, NMR measurements were made directly on model systems containing starch and various gluten amounts. The starch-gluten gels had higher T₂ values than pure starch gels, indicating less swelling of starch granules and absorption of water. This was attributed to less water available to starch in the presence of gluten.     

Water vapor transport properties during staling of bread crumb and crust as affected by heating rate

In order to develop a mathematical model to simulate mass transfer occurring between the crumb and the crust during bread staling, water vapor sorption properties, i.e., moisture diffusivity, WVP and sorption of bread crumb and crust were investigated at 15 °C. Two types of bread baked with two heating rates (7.39 °C/min and 6.32 °C/min) were considered. Sorption and desorption isotherms were determined using Dynamic Vapor Sorption (DVS) and FF and GAB models were applied in the range of 0–0.95 a_w, to fit isotherm curves. Diffusivity was determined from sorption isotherms by using Fick's law and WVP was measured by two methods (gravimetric and from sorption data). Results exhibited maximum values of D_eff in the range of 0.1 and 0.14 g/g d.b. moisture contents. They varied between 0.88 × 10^? 10 and 0.92 × 10^? 10 m²/s for the crust and between 2.24 × 10^? 10 and 2.64 × 10^? 10 m²/s for the crumb, baked respectively at 220 °C and 240 °C. Results of WVP showed that the crust baked at 240 °C was significantly more permeable than the crust baked at 220 °C. This fact was attributed to the difference in porosity and the molecular structure due to heating effects. Also, the presence of steam in the oven atmosphere enhanced the development of higher porosity in the crust, leading to different structures and properties. Moreover, SEM images showed that starch granules were intact and less swelled in the upper crust when baking at 240 °C, resulting in higher WVP.     

Superheated steam drying characteristic and moisture diffusivity of distillers’ wet grains and condensed distillers’ solubles

Samples of distillers’ spent grains were prepared by blending different proportions of distillers’ wet grains (DWG) with condensed distillers’ solubles (CDS). Such samples when dried with superheated steam (SS) at 110, 130 and 160 °C showed typical behaviour of drying in the falling rate period. The overall moisture diffusivity (D_m) increased with a decrease in moisture content under all drying conditions. An increase in moisture diffusivity with respect to the SS temperature was also observed. For all drying conditions, the values of average diffusivities (D_m)_avg non-corrected for shrinkage ranged from 0.52 × 10⁻⁹ to 3.08 × 10⁻⁹ m²/s. For distillers’ spent grains of different ratios of DWG to CDS, the decrease in SS temperature from 160 to 110 °C caused a decrease in (D_m)_avg by 69-82%. Increasing the amount of CDS added to the DWG from 0% to 100% caused an increase in (D_m)_avg by 14-35% for the temperature range tested in this study. The values of (D_m)_avg corrected for shrinkage ranged from 0.17 × 10⁻⁹ to 0.86 × 10⁻⁹ m²/s for all drying conditions studied. The decrease in SS temperature from 160 to 110 °C caused a decrease in (D_m)_avg by 70-74%. Not much differences were observed for the same drying temperatures and different ratios of DWG to CDS. The differences between the values of average overall moisture diffusivity (D_m)_avg corrected and non-corrected for shrinkage were significant, nearly one order of magnitude.     

Modeling water sorption dynamics of cellular solid food systems using free volume theory

Water sorption and dynamical properties of bread crust were studied using gravimetric sorption experiments. Water uptake and loss were measured while relative humidity (RH) was step-wise in- or decreased. Experimental results were compared with Fickian diffusion models and empirical models like the exponential and power-law model. From comparison of experimental sorption curves and the power-law model for short times it followed for all bread crust that the diffusional coefficient n is close to one. It turned out that this is not due to so-called case II diffusion and water transport that is limited by relaxation of the solid material but due to the fact that RH did not instantaneously but gradually increased to the set value.Sorption curves of isotherm experiments could be best described by the Fickian diffusion model for low RH and by the exponential model for large RH. Transport rates depend on moisture content and show a maximum around RH = 0.7, corresponding to a water mass fraction ω₁ = 0.12. Diffusion rates could be well described by free volume theory up to the maximum, but this theory could not explain the strong decrease at higher ω₁. Indications for a local glass-rubber transition at room temperature were found near a water mass fraction ω₁ ≈ 0.09. This corresponds very well to the start of the crisp–non-crisp transition as measured by a sensory panel, but not to the glass-rubber transition at ω₁ ≈ 0.12 as measured by other techniques like Differential Scanning Calorimetry. So it seems that more than one glass-rubber like transitions may be important to describe the properties of heterogeneous cellular food systems.     

Quality and mold control of enriched white bread by combined radio frequency and hot air treatment

This study explored the application of radio frequency (RF) energy in conjunction with conventional hot air treatment to provide uniform heating for control of mold in pre-packaged bread loaf. A 6 kW, 27.12 MHz RF system was used to develop treatment protocols. The treatment parameters were selected based on minimum time-temperature conditions that were required for 4-log reduction of Penicillium citrinum spores while yielding acceptable bread quality. During combined RF and hot air treatments, the core and periphery of the bread loaf were heated together with almost the same heating rate. The maximum temperature difference within one bread slice was less than 5 °C. The moisture contents and water activities of RF treated samples first increased and then decreased compared to those of untreated samples, while firmness increased during the storage for both heat treated and untreated samples, yet the overall differences in sample qualities between RF treated bread samples and control were not significant. Because of better heating uniformity, much lower mean product temperature and shorter holding time were used for control of P. citrinum spores with combined RF and hot air treatment as compared to conventional heating alone. Heating bread to 58 °C or higher resulted in 4-log reduction of P. citrinum spores isolated from moldy bread. The storage life at room temperature (23 °C) was extended by 28 ± 2 days for the treated white bread.     

Sensory Crispness of Crispy Rolls: Effect of Formulation, Storage Conditions, and Water Distribution in the Crust

ABSTRACT:  Crispness is an important sensory quality parameter that strongly influences the acceptability of cellular solid foods such as the crust of many types of breads. Crispness of the bread crust depends particularly on its water content. In this study, the relationship between sensory crispness of crispy rolls and the average water content of the crust was studied for different bread formulations (control, amylase, glucose-oxidase, and protease) and storage conditions (40% and 80% RH). From the different formulations used, only protease treatment increased the crispness of the crust and its retention at both storage conditions. The positive effect of the protease treatment was due to a lower water content of the crust of these breads compared to the other formulations. The relationship between sensory attributes, formulation, and storage conditions was found to be dominated by the dependence on storage conditions. When combining data for low and high humidity storage it showed that crusts with equal water contents could exhibit different scores for crispness. The results led to the hypothesis that a gradient of water content exists within the crust. At high humidity, the crust will take up water from both crumb and environment and a relative smaller gradient of water will exist within the crust. At low humidity on the other hand, the crust will take up water from the crumb only, resulting in a larger gradient of water within the crust.     

Preparation of Fiber and Mineral Enriched Pan Bread by Using Defatted Rice Bran

Wheat flour was gradually replaced with defatted rice bran (DRB) at different levels. Five treatments (T₀ = control i.e. without DRB; T₅ = 5% DRB; T₁₀ = 10% DRB; T₁₅ = 15% DRB; T₂₀ = 20% DRB) were used for bread preparation. Bread loaves were analyzed for chemical composition and sensory evaluation at different storage intervals i.e. S_0, S_24, S_48, S_72, S_96, and S₁₂₀ hours. Protein, ash, fiber, and mineral contents of breads were improved and moisture decreased significantly, whereas fat content showed non-significant effect for increasing levels of defatted rice bran. Maximum protein, ash, fiber, K, Ca, and Mg contents were found in T₂₀ while minimum values were observed in T_0. Moisture and Na contents were decreased by the subsequent addition of rice bran. Treatment T₅ got maximum scores for external characteristics (volume, color of crust, symmetry of form, evenness of bake, character of crust) and internal characteristics (grain, color of crumb, aroma, taste, and texture) of pan bread. From chemical assay and sensory evaluations, the authors concluded that the quality bread can be improved by the addition of 5% DRB having high fiber and mineral content for commercialization.     

Desorption isotherms and thermodynamic properties of sweet corn cultivars (Zea mays L.)

The desorption isotherms and thermodynamic properties of two cultivars of sweet corn were obtained during the drying process of these products. The isotherms were determined by a dynamic method for various temperature and humidity conditions. Equilibrium moisture content (X_eq) data were correlated by the Guggenheim–Anderson–de Boer model and an artificial neural network (ANN) model. These models were fitted to the experimental data. The X_eq for corn grain increased with an increase in the relative humidity at fixed temperature and decreased with an increase in temperature at a constant relative humidity. The experimental data were analysed by a thermodynamic approach to obtain the isosteric heat of desorption (ΔH), differential entropy (ΔS), activation energy (E_a) and Gibbs free energy (ΔG). The ΔH and ΔS increased with a decrease in moisture content, while ΔG decreased exponentially with an increase in X_eq. The Arrhenius equation was used to obtain E_a values, with Supersweet corn having higher E_a.     

Control of amylase and protease activities in a phytase preparation by ampholyte-free preparative isoelectric focusing for unrefined cereal-containing bread

Direct addition of a food additive-grade Aspergillus niger phytase preparation to 30% brown rice flour-added bread ingredients reduced the bread myo-inositol hexaphosphate (IP₆) content. The phytase preparation had protease and amylase activities. Addition of the crude phytase preparation induced bread crust collapse. Protease-free high- and low-amylase phytase fractions were prepared using preparative ampholyte-free isoelectric focusing (autofocusing). Addition of the protease-free low-amylase phytase fraction did not significantly affect loaf volume and did not collapse the crust. Addition of the protease-free high-amylase phytase fraction significantly increased loaf volume approximately 1.5× in the presence of amylase at 193–1029 U. However, addition of 1029 and 2058 U of amylase induced partial and whole bread crust collapse, respectively. Therefore, removal of protease and control of amylase activities in the phytase preparation are crucial in the preparation of brown rice flour-added bread with a low myo-inositol phosphate (IP₆) and good swelling property.     

Moisture Distribution and Microbial Quality of Part Baked Breads as Related to Storage and Rebaking Conditions

Storage conditions of part-baked brown soda bread were studied as related to changes of crust and crumb moisture during rebaking at in-oven temperatures of 180 and 200°C for 10–40 min. Baking loss during rebaking originated solely from the crust area, whereas the moisture content of the crumb remained constant. Packaging in an atmosphere of 40% CO₂ and 60% N₂ and storage at 4°C inhibited microbial contamination for 13 wk. The relationship of moisture content and water activity of part-baked breads followed the characteristic of a sorption isotherm and was mathematically described by either a quadratic or exponential function.     

Phase Transitions and Unfreezable Water Content of Carrots, Reindeer Meat and White Bread Studied using Differential Scanning Calorimetry

Differential scanning calorimetry was used to measure the phase transitions and unfreezable water of carrots, reindeer meat, and white bread. The incipient melting point (T_im), incipient intensive melting point (T′_im), the onset temperature of melting (T_m), latent heat of melting (ΔH_m), specific heat (C_p) and enthalpy (ΔH) were determined from the melting curves. T′_im, T_mΔH_m and ΔH and the unfreezable water were found to be functions of moisture. The T_im, temperatures were ? 39°C, ?33°C, ?40°C; T′_im, ?11.8°C, ?13.3°C ?17.3°C T_m, ? 3.4°C, ? 3.1°C, ? 12.2°C for carrot, reindeer meat, and white bread, respectively. The unfreezable water was 8.3% for carrots, 15.1% for reindeer meat, and 22.5% for white bread, determined from ΔH_m and 3.4%, 6.4% and 2.9%, determined from ΔH. The lowest water detectable from ΔH_m was 26.4% and from ΔH 3.6%.     

Structural Changes in the Dough During the Pre-baking and Re-baking of French Bread Made with Whole Wheat Flour

This study investigates the changes occurring in the dough after the pre-baking and re-baking steps in the preparation of frozen bread (French rolls) made with whole wheat flour. At the end of each step, the different parts of the rolls (inner crumb, outer crumb, and crust) were characterized and compared with the dough. The temperature profile obtained showed that the opening of the cut height occurred when the inner crumb temperature was close to 40 °C. The moisture content of the inner and outer crumbs remained high even after the two baking steps, overcoming problems cited in literature such as weight loss and drying out of the re-baked bread. The color of these parts was not affected by the re-baking step. Using RVA, infrared spectrometry, and scanning electron microscopy analyses, it was found that right after pre-baking, the starch granules in the crumb and crust were not completely gelatinized and gelatinization continued during the re-baking process. After pre-baking, a few isolated intact starch granules remained in the inner and outer crumbs, and a greater amount was found in the crust, as observed by polarized light microscopy. This behavior was also observed after the re-baking step. The DSC and X-ray diffraction results indicated that the amylose–lipid complex present or formed in the dough was still present in all parts of the rolls after the pre-baking and re-baking steps.