Candida krusei development on turbulent flow regimes: Biofilm formation and efficiency of cleaning and disinfection program

In food processing lines or in complex equipment such as pumps or valves, microorganisms are exposed to varying hydrodynamic conditions caused by the flow of liquid food, and biofilms are thus grown under a wide distribution of local hydrodynamic strengths. Using an industrially relevant strain of Candida krusei, we demonstrated that biofilms formed on stainless steel for 4 days at Reynolds (Re) numbers ranging from 294,000 to 1.2 × 10⁶ proceeds through three distinct developmental phases. These growth phases transform adherent blastospores to well-defined cellular communities encased in an extracellular matrix and biofilm formation increases when increasing Reynolds number and time. In all growth phases, the morphology of C. krusei biofilm revealed the influence of hydrodynamic drag. Indeed, we study the effect of cleaning and sanitation procedure in the control of turbulent flow-generated biofilm. This procedure involves alkali (NaOH 0.5%) and sodium hypochlorite (500 ppm). In terms of total biofilm mass, removal decreases with increasing biofilm age. The largest reduction post-treatment (between 57% and 62%) was observed, to all Reynolds numbers, on 24 and 48 h-old biofilms. Removal was between 39% and 46% on 72 h-old biofilms and was close to 30% for all Reynolds numbers on 96 h-old biofilm.     

Inactivation of Escherichia coli O157:H7 on stainless steel upon exposure to Paenibacillus polymyxa biofilms

We investigated the potential use of biofilm formed by a competitive-exclusion (CE) microorganism to inactivate Escherichia coli O157:H7 on a stainless steel surface. Five microorganisms showing inhibitory activities against E. coli O157:H7 were isolated from vegetable seeds and sprouts. The microorganism with the greatest antimicrobial activity was identified as Paenibacillus polymyxa (strain T5). In tryptic soy broth (TSB), strain T5 reached a higher population at 25 °C than at 12 or 37 °C without losing inhibitory activity against E. coli O157:H7. When P. polymyxa (6 log CFU/mL) was co-cultured with E. coli O157:H7 (2, 3, 4, or 5 log CFU/mL) in TSB at 25 °C, the number of E. coli O157:H7 decreased significantly within 24 h. P. polymyxa formed a biofilm on stainless steel coupons (SSCs) in TSB at 25 °C within 24 h, and cells in biofilms, compared to attached cells without biofilm formation, showed significantly increased resistance to a dry environment (43% relative humidity [RH]). With the exception of an inoculum of 4 log CFU/coupon at 100% RH, upon exposure to biofilm formed by P. polymyxa on SSCs, populations of E. coli O157:H7 (2, 4, or 6 log CFU/coupon) were significantly reduced within 48 h. Most notably, when E. coli O157:H7 at 2 log CFU/coupon was applied to SSCs on which P. polymyxa biofilm had formed, it was inactivated within 1 h, regardless of RH. These results will be useful when developing strategies using biofilms produced by competitive exclusion microorganisms to inactivate foodborne pathogens in food processing environments.     

Biofilm-producing ability of Staphylococcus aureus isolates from Brazilian dairy farms

This study aimed to investigate the in silico biofilm production ability of Staphylococcus aureus strains isolated from milking parlor environments on dairy farms from São Paulo, Brazil. The Staph. aureus isolates were obtained from 849 samples collected on dairy farms, as follows: milk from individual cows with subclinical mastitis or history of the disease (n = 220); milk from bulk tank (n = 120); surfaces of milking machines and utensils (n = 389); and milk handlers (n = 120). Thirty-one Staph. aureus isolates were obtained and categorized as pulsotypes by pulsed-field gel electrophoresis and submitted to assays for biofilm formation on polystyrene, stainless steel, rubber, and silicone surfaces. Fourteen (45.2%) pulsotypes were considered producers of biofilm on the polystyrene microplate assay, whereas 13 (41.9%) and 12 (38.7%) pulsotypes were biofilm producers on stainless steel and rubber, respectively. None of the pulsotypes evaluated produced biofilms on silicone. Approximately 45% of Staph. aureus pulsotypes isolated from different sources on dairy farms showed the ability to produce biofilms in at least one assay, indicating possible persistence of this pathogen in the milking environment. The potential involvement of Staph. aureus in subclinical mastitis cases and its occurrence in milk for human consumption emphasize the need to improve hygiene practices to prevent biofilm formation on the farms studied.     

Resistance of pathogenic bacteria on the surface of stainless steel depending on attachment form and efficacy of chemical sanitizers

Various bacteria including food spoilage bacteria and pathogens can form biofilms on different food processing surfaces, leading to potential food contamination or spoilage. Therefore, the survival of foodborne pathogens (Escherichia coli O157:H7, Listeria monocytogenes, Salmonella typhimurium, Staphylococcus aureus, Cronobacter sakazakii) in different forms (adhered cells, biofilm producing in TSB, biofilm producing at RH 100%) on the surface of stainless steel and stored at various relative humidities (RH 23%, 43%, 68%, 85%, and 100%) at room temperature for 5 days was investigated in this study. Additionally, the efficacy of chemical sanitizers (chlorine-based and alcohol-based commercial sanitizers) on inhibiting various types of biofilms of E. coli O157:H7 and S. aureus on the surface of stainless steel was investigated. The number of pathogens on the surface of stainless steel in TSB stored at 25 °C for 7 days or RH 100% at 25 °C for 7 days was significantly increased and resulted in the increase of 3 log₁₀ CFU/coupon after 1 day, and these levels were maintained for 7 days. When stainless steel coupons were stored at 25 °C for 5 days, the number of pathogens on the surface of stainless steel was significantly reduced after storage at RH 23%, 43%, 68%, and 85%, but not at 100%. When the bacteria formed biofilms on the surface of stainless steel in TSB after 6 days, the results were similar to those of the attached form. However, levels of S. aureus and C. sakazakii biofilms were more slowly reduced after storage at RH 23%, 43%, 68%, and 85% for 5 days than were those of the other pathogens. Formation of biofilms stored at RH 100% for 5 days displayed the highest levels of resistance to inactivation. Treatment with the alcohol sanitizer was very effective at inactivating attached pathogens or biofilms on the surface of stainless steel. Reduction levels of alcohol sanitizer treatment ranged from 1.91 to 4.77 log and from 4.35 to 5.35 log CFU/coupon in E. coli O157:H7 and S. aureus, respectively. From these results, the survival of pathogens contaminating the surfaces of food processing substrates such as stainless steel varied depending on RH and attachment form. Also, alcohol-based sanitizers can be used as a potential method to remove microbial contamination on the surfaces of utensils, cooking equipment, and other related substrates regardless of the microbial attached form.     

Microbial biofilm detection on food contact surfaces by macro-scale fluorescence imaging

Hyperspectral fluorescence imaging methods were utilized to evaluate the potential detection of pathogenic bacterial biofilm formations on five types of food contact surface materials: stainless steel, high-density polyethylene (HDPE), plastic laminate (Formica), and two variations of polished granite. The main objective of this study was to determine a minimal number of spectral fluorescence bands suitable for detecting microbial biofilms on surfaces commonly used to process and handle food. Spots of biofilm growth were produced on sample surfaces by spot-inoculations of pathogenic Escherichia coli O157:H7 and Salmonella followed by room temperature storage for 3 days. Subsequently, hyperspectral fluorescence images were acquired from 421 to 700 nm using ultraviolet-A excitation. Both E. coli O157:H7 and Salmonella biofilms emitted fluorescence predominantly in the blue to green wavelengths with emission maxima at approximately 480 nm. A single-band image at 559 nm was able to detect the biofilm spots on stainless steel. On HDPE and granite, algorithms using different two-band ratios provided better separation of the biofilm spots from background areas than any single-band images did. The biofilm spots on stainless steel, HDPE, and granite could be detected with overall detection rate of 95%. On Formica, too many false positives were present to accurately determine an effective biofilm detection rate. This may have been due to the lower cell population density that was observed for the biofilm spots grown on Formica (approximately 4.3–6.4 log cfu cm⁻²) as compared to the other surfaces. These findings can be incorporated into developing portable hand-held imaging devices for sanitation inspection of food processing surfaces.     

A predictive model for heat inactivation of Listeria monocytogenes biofilm on buna-N rubber

The purpose of this study was to develop a predictive model for the heat inactivation of Listeria monocytogenes in monoculture (strains Scott A and 3990) and with competing bacteria (Pseudomonas sp. and Pantoea agglomerans) formed on buna-N rubber with and without the presence of food-derived soil. Biofilms were produced on rubber disks in dilute Tryptic Soy broth (dTSB) with incubation for 48 h at 25 °C. Duplicate biofilm samples were heat treated for 1, 3, 5, and 15 min at 70, 72, 75, 77 and 80 °C and tested for survivors using enrichment media. The experiment was repeated six times. A predictive model was developed and plots were generated showing the percent probability of L. monocytogenes inactivation in biofilms after heat treatment. For example, to achieve a 95% probability level of complete inactivation required heat treatment of 76 °C for 6 min. The predicted model was validated using a five-strain cocktail of L. monocytogenes. The validated prediction model indicates that with proper maintenance of the time/temperature controls L. monocytogenes in biofilms on rubber surfaces will be inactivated. This model can be used as a tool in the selection of hot water sanitation processes for rubber surfaces.     

Addition of ethanol to supercritical carbon dioxide enhances the inactivation of bacterial spores in the biofilm of Bacillus cereus

Supercritical carbon dioxide (SC-CO₂) was used to inactivate Bacillus cereus spores inside biofilms, which were grown on stainless steel. SC-CO₂ treatment was tested using various conditions, such as pressure treatment (10–30 MPa), temperature (35–60 °C), and time (10–120 min). B. cereus vegetative cells in the biofilm were completely inactivated by treatment with SC-CO₂ at 10 MPa and at 35 °C for 5 min. However, SC-CO₂ alone did not inactivate spores in biofilm even after the treatment time was extended to 120 min. When ethanol was used as a cosolvent with SC-CO₂ in the SC-CO₂ treatment using only 2–10 ml of ethanol in 100 ml of SC-CO₂ vessel for 60–90 min of treatment time at 10 MPa and 60 °C, B. cereus spores in the biofilm were found to be completely inactivated in the colony-forming test. We also assessed the viability of SC-CO₂-treated bacterial spores and vegetative cells in the biofilm by staining with SYTO 9 and propidium iodide. The membrane integrity of the vegetative cells was completely lost, while the integrity of the membrane was still maintained in most spores. However, when SC-CO₂ along with ethanol was used, both vegetative cells and spores lost their membrane integrity, indicating that the use of ethanol as a cosolvent with SC-CO₂ is efficient in inactivating the bacterial spores in the biofilm.     

Evaluation of anti-Listeria meat borne Lactobacillus for biofilm formation on selected abiotic surfaces

The ability of meat borne anti-Listeria Lactobacillus to form biofilms under different in vitro conditions and on abiotic surfaces was investigated. Biofilm formation by the adhesion to polystyrene microtiter plates was determined, this being higher for Lactobacillus curvatus CRL1532 and CRL705 and Lactobacillus sakei CRL1862. The physicochemical properties of the cell surface were relatively hydrophilic and acidic in character; L. sakei CRL1862 exhibiting the strongest autoaggregation. The adhesion of lactobacilli to stainless steel (SS) and polytetrafluoroethylene (PTFE) supports at 10 °C was found to be maximal for L. sakei CRL1862 on SS after 6 days. When biofilm architecture was characterized by epifluorescence and SEM, L. sakei CRL1862 homogeneously covered the SS surface while cell clusters were observed on PTFE; the extracellular polymeric substance matrix adapted to the topography and hydrophilic/hydrophobic characteristics of each material. The feasibility of L. sakei CRL1862 to form biofilm on materials used in meat processing highlights its potential as a control strategy for Listeria monocytogenes biofilms.     

Mixed species biofilms of Listeria monocytogenes and Lactobacillus plantarum show enhanced resistance to benzalkonium chloride and peracetic acid

We investigated the formation of single and mixed species biofilms of Listeria monocytogenes strains EGD-e and LR-991, with Lactobacillus plantarum WCFS1 as secondary species, and their resistance to the disinfectants benzalkonium chloride and peracetic acid. Modulation of growth, biofilm formation, and biofilm composition was achieved by addition of manganese sulfate and/or glucose to the BHI medium. Composition analyses of the mixed species biofilms using plate counts and fluorescence microscopy with dual fluorophores showed that mixed species biofilms were formed in BHI (total count, 8-9 log₁₀ cfu/well) and that they contained 1-2 log₁₀ cfu/well more L. monocytogenes than L. plantarum cells. Addition of manganese sulfate resulted in equal numbers of both species (total count, 8 log₁₀ cfu/well) in the mixed species biofilm, while manganese sulfate in combination with glucose, resulted in 1-2 log₁₀ more L. plantarum than L. monocytogenes cells (total count, 9 log₁₀ cfu/well). Corresponding single species biofilms of L. monocytogenes and L. plantarum contained up to 9 log₁₀ cfu/well. Subsequent disinfection treatments showed mixed species biofilms to be more resistant to treatments with the selected disinfectants. In BHI with additional manganese sulfate, both L. monocytogenes strains and L. plantarum grown in the mixed species biofilm showed less than 2 log₁₀ cfu/well inactivation after exposure for 15 min to 100 μg/ml benzalkonium chloride, while single species biofilms of both L. monocytogenes strains showed 4.5 log₁₀ cfu/well inactivation and single species biofilms of L. plantarum showed 3.3 log₁₀ cfu/well inactivation. Our results indicate that L. monocytogenes and L. plantarum mixed species biofilms can be more resistant to disinfection treatments than single species biofilms.     

Initial adhesion of Listeria monocytogenes to solid surfaces under liquid flow

Some strains of the food borne pathogen Listeria monocytogenes persist in food processing environments. The exact reason behind this phenomenon is not known, but strain differences in the ability to adhere to solid surfaces could offer an explanation. In the present work, initial adhesion of nine strains of L. monocytogenes was investigated under liquid flow at two levels of shear stress on six different surfaces using a flow chamber set-up with microscopy measurements. The surfaces tested were glass and PVC, and glass coated with beef extract, casein, and homogenised and unhomogenised milk. In addition, the effect of prior environmental stress (5% NaCl, low nutrient availability) on initial adhesion was investigated. The hydrophobicity of the investigated surfaces was determined by contact angle measurements and the surface properties of the investigated L. monocytogenes strains were determined using Microbial Adhesion To Solvents (MATS). All surfaces with the exception of PVC were found to be hydrophilic. Strain differences were found to significantly influence the initial adhesion rate (IAR) of all nine strains to all the surfaces (p < 0.05) at both low and high shear stress. Furthermore, there was a significant effect of the surfaces tested (p < 0.05) in the adhesion ability of almost all strains. The IAR was affected by flow rate (shear stress) as seen by a decrease in adhesion at high shear stress for most strains. A significant effect of interactions between strain-surface and strain-shear stress (p < 0.001) was observed but not of interactions between surface-shear stress. No correlation between surface hydrophobicity and IAR was observed. Addition of 5% NaCl during propagation resulted in a decrease in IAR whilst propagation in low nutrient media caused an increase indicating a general change in surface characteristics under these conditions. Known persisting strains did not display general better adherence.     

A rheological characterization of semi-solid dairy systems

Dispersions of cross-linked starch in full fat milk, taken as models of custard model systems, have been characterized by different rheological means: viscoelastic measurements, classical flow measurements and ‘vane’ rheometry. From viscosity measurements, the flow behaviour was described within the shear rate range 0.01–100 s⁻¹. The flow curves were fitted using the Herschell–Bulkley equation over the shear rate range 0.1–100 s⁻¹ while a deviation was found towards the low shear rate range, making the determination of the yield stress non realistic. Instead, measurements with the ‘vane’ device in low shear conditions provided a way to estimate the yield stress, at rest and after shearing, but the entire flow curve was not described. From the viscoelastic measurements at low strain amplitude, the mechanical spectra were obtained. Linearity tests beyond the linearity limits provided the critical stress corresponding to the G′–G″ cross-over. The parameters obtained from these different rheological methods are discussed.     

Use of titanium dioxide (TiO2) photocatalysts as alternative means for Listeria monocytogenes biofilm disinfection in food processing

The aim of this work was to study the photocatalytic activity of titanium dioxide (TiO₂) against Listeria monocytogenes bacterial biofilm. Different TiO₂ nanostructured thin films were deposited on surfaces such as stainless steel and glass using the doctor-blade technique. All the surfaces were placed in test tubes containing Brain Heart (BH) broth and inoculated with L. monocytogenes. Test tubes were then incubated for 10 days at 16 °C in order to allow biofilm development. After biofilm formation, the surfaces were illuminated by ultraviolet A light (UVA; wavelength of 315-400 nm). The quantification of biofilms was performed using the bead vortexing method, followed by agar plating and/or by conductance measurements (via the metabolic activity of biofilm cells). The presence of the TiO₂ nanoparticles resulted in a fastest log-reduction of bacterial biofilm compared to the control test. The biofilm of L. monocytogenes for the glass nanoparticle 1 (glass surface modified by 16% w/v TiO₂) was found to have decreased by 3 log CFU/cm² after 90 min irradiation by UVA. The use of TiO₂ nanostructured photocatalysts as alternative means of disinfecting contaminated surfaces presents an intriguing case, which by further development may provide potent disinfecting solutions. Surface modification using nanostructured titania and UV irradiation is an innovative combination to enhance food safety and economizing time and money.     

Evaluation of rheological properties of date syrup

Rheological behavior of date syrup is an important factor affecting the efficiency of sugar production and refining processes such as boiling, crystallization, separation and pumping. A rotational viscometer was used to characterize the flow behavior of date syrup solution at four different temperatures (20 °C, 40 °C, 60 °C and 80 °C) and four concentrations (17, 24, 31 and 39 °Brix). The samples were subjected to a programmed shear rate increasing from 10 to 100 s⁻¹ in 2 min, held constant at 100 s⁻¹ for 10 min and linearly decreasing to 10 during 2 min. The power law model was fitted to shear stress vs. shear rate data to obtain the consistency coefficient (m) and the flow behavior index (n). Both m and n were sensitive to changes in temperature and concentration. The apparent viscosity increases with increasing concentration of date syrup and a decrease in temperature.     

Roles of outer membrane protein W (OmpW) on survival,morphology, and biofilm formation under NaCl stresses in Cronobacter sakazakii

Cronobacter sakazakii is an important foodborne pathogen associated with rare but severe infections through consumption of powdered infant formula. Tolerance to osmotic stress in Cronobacter has been described. However, the detailed factors involved in tolerance to osmotic stress in C. sakazakii are poorly understood. In this study, roles of outer membrane protein W (OmpW) on survival rates, morphologic changes of cells, and biofilm formation in C. sakazakii under different NaCl concentrations between wild type (WT) and OmpW mutant (ΔOmpW) were determined. The survival rates of ΔOmpW in Luria-Bertani medium with 3.5% or 5.5% NaCl were reduced significantly, and morphological injury of ΔOmpW was significantly increased compared with survival and morphology of WT. Compared with biofilm formation of the WT strain, biofilms in ΔOmpW were significantly increased in Luria-Bertani with 3.5% or 5.5% NaCl using crystal violet staining assay after 48 and 72 h of incubation. Detection of biofilms using confocal laser scanning microscopy and scanning electron microscopy further confirmed the changes of biofilm formation under different NaCl stresses. This study demonstrates that OmpW contributes to survival of cells in planktonic mode under NaCl stresses, and biofilm formation is increased in ΔOmpW in response to NaCl stress.     

Orientation and detachment dynamics of Bacillus spores from stainless steel under controlled shear flow: modelling of the adhesion force

Shear-flow induced spore detachment was performed under well-controlled laminar flow conditions, in a specially-designed shear stress flow chamber. By comparing detachment profiles of a panel of four strains, belonging to the B. cereus group (B. cereus and B. thuringiensis) and to less related Bacillus species (B. pumilus), it was shown that the spore ability of attaching to stainless steel, probed under dynamic conditions, was mainly affected by the presence (and number) of appendages. Adhesion force between the B. cereus 98/4 strain and stainless steel was quantified at nanoscale. To this aim, detachment results were combined with a theoretical modelling, based on the balance of hydrodynamic forces and torque exerted over a simplified spore model with a spherical form. The wall shear stress, required to remove 50% of the spores initially attached to stainless steel, was determined. On this basis, an adhesion force of 930 ± 390 pN was obtained. Real-time re-orientation of B. cereus 98/4 spores was experimentally established, by using a high-speed camera for tracking the motions of individual spores with high temporal and spatial resolution. Even though tethered to stainless steel without any detachment occurring, spores kept mobile on the substratum, probably due to the existence of discrete bonds or local clusters of anchoring sites, and tended to re-orientate in the flow direction, for minimizing hydrodynamic forces and torque exerted by fluid flow. A significant heterogeneity within the population was also observed, with the co-existence of both moving and immobile spores.     

Rheological properties of ultraviolet-irradiated and thermally pasteurized Yankee pineapple juice

The rheological behaviour of Yankee pineapple juice was examined for the effect of ultraviolet (UV) irradiation (53.42 mJ/cm²) and compared with untreated juice and a thermally pasteurized (80 °C for 10 min) juice. A rheological test was performed on all types of juice in the temperature range 5 °C to 25 °C using a concentric cylinder rheometer at a shear rate range of 10–290 s⁻¹. The comparative analysis found that the best flow curves were described by the Bingham model with an initial shear stress. The entangled pulps in the juices prevented free flow at zero shear rate. There was no significant variation between the plastic viscosities of the untreated and UV-irradiated juice at all temperatures. The activation energy (E_a) of the untreated, UV-irradiated and thermally pasteurized juice was 6.80, 8.19 and 8.50 kJ/mol respectively.     

In situ characterization and analysis of Salmonella biofilm formation under meat processing environments using a combined microscopic and spectroscopic approach

Salmonella biofilm on food-contact surfaces present on food processing facilities may serve as a source of cross-contamination. In our work, biofilm formation by multi-strains of meat-borne Salmonella incubated at 20 °C, as well as the composition and distribution of extracellular polymeric substances (EPS), were investigated in situ by combining confocal laser scanning microscopy (CLSM), scanning electron microscope (SEM), attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) and Raman spectroscopy. A standard laboratory culture medium (tryptic soy broth, TSB) was used and compared with an actual meat substrate (meat thawing-loss broth, MTLB). The results indicated that Salmonella grown in both media were able to form biofilms on stainless steel surfaces via building a three-dimensional structure with multilayers of cells. Although the number of biofilm cells grown in MTLB was less than that in TSB, the cell numbers in MTLB was adequate to form a steady and mature biofilm. Salmonella grown in MTLB showed “cloud-shaped” morphology in the mature biofilm, whereas when grown in TSB appeared “reticular-shaped”. The ATR-FTIR and Raman analysis revealed a completely different chemical composition between biofilms and the corresponding planktonic cells, and some important differences in biofilms grown in MTLB and in TSB. Importantly, our findings suggested that the progress towards a mature Salmonella biofilm on stainless steel surfaces may be associated with the production of the EPS matrix, mainly consisting of polysaccharides and proteins, which may serve as useful markers of biofilm formation. Our work indicated that a combination of these non-destructive techniques provided new insights into the formation of Salmonella biofilm matrix.     

Desiccation of adhering and biofilm Listeria monocytogenes on stainless steel: Survival and transfer to salmon products

The foodborne bacterial pathogen, Listeria monocytogenes, commonly contaminates foods during processing, where the microorganisms are potentially subjected to low relative humidity (RH) conditions for extended periods of time. The objective of this study was to examine survival during desiccation (43% RH and 15 °C) of biofilm L. monocytogenes N53-1 cells on stainless steel coupons and to assess subsequent transfer to salmon products. Formation of static biofilm (2 days at 100% RH and 15 °C) prior to desiccation for 23 days significantly (P < 0.05) improved survival of cells desiccated in initial low salt concentrations (0.5%) compared to the survival for non-biofilm cells also desiccated in low salt, indicating the protective effect of the biofilm matrix. Osmoadaptation of cells in 5% NaCl before formation of the static biofilm significantly (P < 0.05) increased long-term desiccation survival (49 days) irrespectively of the initial salt levels (0.5% and 5% NaCl). The efficiency of transfer (EOT) of desiccated biofilm cells was significantly (P < 0.05) lower than EOTs for desiccated non-biofilm bacteria, however, as biofilm formation enhanced desiccation survival more bacteria were still transferred to smoked and fresh salmon. In conclusion, the current work shows the protective effect of biofilm formation, salt and osmoadaptation on the desiccation survival of L. monocytogenes, which in turn increases the potential for cross-contamination during food processing.     

Steady shear flow properties of wild sage (Salvia macrosiphon) seed gum as a function of concentration and temperature

The steady shear flow properties of dispersions of a new potential hydrocolloid, sage seed gum (SSG), were determined as a function of concentration (0.5–2% w/w), and temperature (20–50 °C). SSG dispersions exhibited strong shear-thinning behavior at all conditions tested, which was even more pronounced than commercial hydrocolloids like xanthan, guar gum and locust bean gum. Different time-independent rheological models were used to fit the experimental data, although the Herschel–Bulkley model (H–B) was found the best model to describe steady shear flow behavior of SSG. An increase in gum concentration led to a large increase in yield stress and consistency coefficient values, whereas there was no definite trend with an increase in temperature. On the other hand, the above-mentioned increases in concentration and temperature did not yield a clear evolution of the shear-thinning characteristics of SSG dispersions. An Arrhenius-type model was also used to describe the effect of temperature. The activation energy (E_a) appeared in the range of 3949–16384 J/mol, as concentration increased from 0.5 to 2%, at a shear rate of 100 s⁻¹. The yield stress values estimated by viscoplastic rheological models were much higher than the data determined by stress ramp method. Apparent viscosity of SSG surpassed many commercial hydrocolloids such as guar gum, locust bean gum, Tara gum, fenugreek gum and konjac gum at the same conditions, which suggest it as a very good stabilizer in food formulations.