五种苹果质构测定方法的比较及与感官评价的相关性分析

为比较5种苹果质构不同测定方法,采用剪切法、穿刺法和TPA压缩法测定了辽宁地区5种主栽苹果的质构特征,同时研究了不同测试条件对测定指标的影响,并分析了仪器测定与感官评价结果的相关性。结果表明剪切、穿刺和TPA压缩均能测得苹果的硬度特征,并区分不同品种间的差异,去皮果肉硬度大小依次为王林 > 红富士 > 国光 > 乔纳金 > 黄元帅;穿刺法检测显示果皮对苹果质构有显著影响(P<0.05),且不同穿刺部位的质构差异显著(P<0.05),其中果顶部位穿刺硬度值最大,其次为果肩和赤道;与感官评价相关性分析显示最大穿刺模量与感官脆性极显著相关(r=0.638,P<0.01),可作为苹果脆性的表征指标;对TPA法,随着压缩形变量增加,苹果硬度测定值显著增加(P<0.05),而内聚性显著减小(P<0.05),且形变量为25%时与感官评价相关性最好。本研究表明穿刺法和TPA法均适用于苹果质构的测定,果皮及穿刺位点对穿刺影响显著,最大穿刺模量可用于表征苹果脆性指标;压缩形变量为25%时TPA法所测得质构参数与感官评价结果相关性最好。     

Texture of Pecans Measured by Sensory and Instrumental Means

The texture of pecans (Carya illinoensis) from four cuhivars (Barton, Mahan, Western and Wichita) was analyzed using sensory and instrumental methods. Sensory hardness, flexibility and crispness were rated by trained panelists. Ranking of hardness and crispness was also carried out. A Texture Analyser TAXT2 was used for 50% compression, texture profile analysis (TPA), puncture and bending. Puncture and 50% compression gave best reproducibility, least variability and agreement with sensory data. The most relevant TPA parameters were cohesiveness, elasticity and fracturabihty. Parameters from the bending test did not indicate texture of the pecans suitably.     

Texture and Sensory Evaluations of Frankfurters Made with Different Formulations and Processes

Objective tests for frankfurter textural properties were investigated by varying formulations and processing parameters and then correlating nine sensory textural attributes with eleven instrumental methods. The most discriminative instrumental parameters were compressive force at failure, compressive force at 50% deformation without failure, and maximum shear force. High correlations (r > 0.80) were found between compressive forces or shear force and sensory elasticity, firmness, and chewiness; stress relaxation and elasticity; total expressible fluid and juiciness; and expressible moisture and wateriness. Elastic recovery did not correlate with any sensory characteristic.     

RELATIONSHIP BETWEEN INSTRUMENTAL AND SENSORY ANALYSIS OF TEXTURE AND COLOR OF POTATO CHIPS

The instrumental and sensory analysis of the texture and color of commercial potato chips were compared. The instrumental measurement was a puncture test with an Intron Universal Testing Machine, and the parameters used were fracture force, deformation and stiffness. The instrumental color quantification was a computerized video image analysis technique, and the color was expressed as L*a*b* values. Sensory evaluation of texture and color was performed by a sensory panel especially trained in evaluating potato chips. The sensory attributes were "hardness", "chewiness", "crunchiness", and "tenderness" for texture analysis, and "yellow color", "burnt aspect", "sugar colored aspect" and "transparency" for color analysis. The factor analysis of the sensory attributes indicated that texture can be divided into two principal components, one represented by "hardness", "crunchiness" and "chewiness", and the other by "tenderness" alone. The factor analysis of the color can be divided into two principal components, one including "yellow color" and "burnt aspect", and the other "sugar colored aspect" and "transparency". Discriminant analysis showed that "tenderness" and "crunchiness" could predict correctly over 90% of the data. Fracture force correlated well with all of the sensory attributes (R² > 0.76), and L* with the sensory color attributes (R² > 0.79). The "Tenderness" was the individual sensory attribute which had the highest correlation (R²= 0.95) with fracture force.     

Effect of Steam-Assisted Hybrid Cooking on Textural Quality Characteristics,Cooking Loss,and Free Moisture Content of Beef

Semitendinosus muscles were cooked in a steam-assisted hybrid oven and also convection ovens at three different oven temperatures (180, 210, and 240°C) until three different end point temperatures [65°C (medium-rare), 72°C (medium), 80°C (medium-well)] were reached. Textural properties of cooked beef were investigated by the Warner Bratzler shear test and texture profile analysis. Cooking loss and free moisture content of muscle tissue was determined for each cooking condition. In addition, sensory analysis was carried out in order to compare with the instrumental results and correlations between instrumental texture parameters and sensory results. Steam-assisted hybrid oven cooking of beef resulted in a tougher texture, higher cooking loss, and lower free moisture content than convection cooking. High correlation coefficients (r² > 0.70) were observed between instrumental texture measurements and sensory results for all ovens, especially in terms of tenderness. The free moisture content and adhesiveness values were also correlated well with juiciness (r² > 0.70) for all oven types.     

SENSORY AND MECHANICAL ATTRIBUTES OF GEL TEXTURE

Eighteen experienced judges evaluated the texture of gels varying in gelatin concentration (22-45 g/L) in terms of firmness by oral and manual shear and compression, cohesiveness, and extent of breakdown in the mouth. Manual compression and biting with the front teeth discriminated well across gel concentrations. All sensory measures except extent of breakdown increased with gelatin concentration. Instron (IUTM) measurements showed that increasing gelatin concentration resulted in an increase in maximum force and force/deformation, but had little effect on deformation at yield and rupture or in elasticity and cohesiveness. Results from mechanical measurements varied with the type of force applied (compression, shear or puncture), the loading rate (50 or 200 mm/min), and the extent of deformation attained (40–90%). The highest discrimination across gel concentrations was achieved with shear force at a rate of 200 mm/min and at greater deformations. Sensory responses correlated most highly with the following IUTM measurements: (1) Compression forces at yield and at deformations of 70 and 85% at the higher crosshead speed; (2) Compression forces below the yield point at the lower crosshead speed; and (3) Shear forces measured at maximum deformation (90%) at 200 mm/min.     

豆腐质构的感官评定与仪器评价的相关性分析

为探索最能反映豆腐感官质构的仪器指标,选取了四种市售鲜豆腐作为研究对象,采用豆腐质构仪器测定中常用的二次压缩和插入试验进行仪器指标测定,同时进行分析型感官质构评定试验,并对两者测定指标之间进行了相关性分析。结果表明:压缩试验中的5项仪器指标（硬度、弹性、咀嚼性、凝聚性及胶粘性）除凝聚性外,其余4项均与感官质构评价指标间存在较好的相关性,其中硬度与感官质构硬度相关性显著（r=0.952,P<0.05）;插入实验中的3项仪器指标（表观破断应变ε、表观破断应力σ、表观弹性率E）均与感官质构评价指标间存在较好的相关性,其中表观破断应力σ与感官硬度、细腻性之间相关性显著（r=0.989, ?0.978,P<0.05）。综合考虑,仪器评价指标中的硬度及表观破断应力可以代替感官评定指标客观准确的评价豆腐质构的好坏,在豆腐的实际生产中及豆腐质构的科研中也可考虑仅选用这两个仪器指标。     

Physical measures of sensory texture in thawed sea scallop meat

An instrumental method of texture measurement for scallop (Placopecten magellanicus) meats after frozen storage has been developed to replace the sensory method. Non-destructive and destructive compressive forces at 50 and 80% deformation respectively and shear peak force gave significant correlation coefficients of 0.89, 0.78 and 0.82 respectively with sensory evaluation of the cooked meats. The coefficient of variation associated with the compression method was smaller, however, than with the shear method.     

Textural Measurements and Product Quality of Restructured Sweetpotato French Fries

The objectives of this research were to investigate the applicability of using an alginate-calcium gelling system to produce a French fry-type product using high beta-carotene sweetpotato puree as the main ingredient and to compare four instrumental methods of texture measurement for this product type in relation to sensory textural properties. Sweetpotato puree made with Jewel cultivar sweetpotatoes was restructured using optimized alginate-calcium concentrations. A consumer panel scored sweetpotato fries containing 0.35 g alginate/100 g and 0.5 g CaSO₄/100 g highest for appearance, texture, flavor, and overall acceptability. A trained texture profile panel described the product with 15 texture notes. Of these attributes, nine sensory notes describing hardness, mastication shear, cohesiveness, springiness, moistness, and oiliness were highly correlated with some instrumental parameters (r=0.79-0.92) for measuring textural properties. The instrumental methods evaluated were instrumental texture profile analysis, three-point bending force, Kramer shear force, and puncture force. Among these instrumental methods, Kramer shear appeared to be the method of choice because of method's simplicity and high precision (coefficient of variation ≤10%).     

MEAT TEXTURE CHARACTERISATION: COMPARISON OF CHEWING PATTERNS, SENSORY AND MECHANICAL MEASURES

The texture of beef, varied by muscle (semimembranosus and semitendinosus), myofibrillar status (ageing time and cold-shortening) and cooking temperature (60C and 80C), was determined by a sensory profile, chewing patterns (determined from surface electromyograms) and mechanical properties of the cooked meats measured at low and high deformation in compression, shear (cutting) and by dynamic (viscoelasticity) tests. The meat factors were discriminated differently by the different measurements showing that they detected differently the various origins of meat texture. Only the maximum modulus before 70% compression and sensory assessments and EMG gave discrimination among all the meat factors. Two main sensory dimensions were found from the profile: juiciness and tenderness with a third related to the determination of elasticity. Juiciness, which varied most between cooking temperatures, was better related to mechanical parameters at low strain rather than high strain and to initial muscular activity rather than later muscular activity. Conversely, tenderness was better related to high strain mechanical properties and to measurements in the middle or later part of the mastication process suggesting that tenderness assessment requires structural disintegration of meat.     

The influence of biochemical differences on the variation in tenderness of M. longissimus dorsi of Belgian Blue steers managed homogenously pre and post-slaughter

The objective of this experiment was to determine the contribution of some biochemical processes of postmortem muscle to the variation in tenderness of beef from Belgian Blue bull cross Holstein-Friesian steers (n=10). These animals were managed optimally from conception to consumption with the aim of reducing tenderness variation. The M. longissimus dorsi (LD) from the left hand side (LHS) and the right hand side (RHS) were analysed for variation in tenderness using Bartletts test. The quality measurements included pH, temperature, Warner Bratzler shear force, sensory tenderness, chemical composition and sarcomere length. Biochemical measurements included myofibrilar proteolysis, glycolytic potential, adenine/inosine ratio and collagen content. No difference for variances or means were observed between LHS and RHS for chemical, quality or biochemical attributes. Biochemical variation was greater than the variation observed in most of the quality attributes measured. Proteolysis was the main biochemical contributor to the variation in shear force tenderness after 2 and 7 as postmortem, but not sensory tenderness. Glycolysis levels and adenine/inosine ratio explained much of the variation in sensory tenderness, but not WBSF. Collagen content in the LD muscles did not explain variation in shear force or sensory tenderness. This would suggest biochemical variation is one of the main contributors to variation in tenderness of beef managed optimally pre- and post-slaughter.     

响应面法优化木瓜蛋白酶嫩化牛肉工艺

为了提高牛肉品质,采用木瓜蛋白酶对牛肉嫩化工艺进行优化。以酶浓度、pH、处理温度、处理时间为考察因素,在单因素实验的基础上,采用Box-Behnken实验设计进行4因素3水平的响应面分析优化,确定最佳牛肉嫩化条件为:酶浓度0.07%,pH6.9,处理温度60℃,处理时间1.8 h。在此最佳嫩化条件下牛肉的持水率、弹性、内聚性及咀嚼性较对照组显著提高(p<0.01),烹饪失水率、剪切力和硬度较对照组显著降低(p<0.01),感官评价达到94.20分,较优化前提高了39.56%。初步机制研究发现木瓜蛋白酶可以降解牛肉肌纤维蛋白来提高牛肉嫩度。     

Characterization of beef semimembranosus and adductor muscles from US and Mexican origin

The purpose of this study was to compare the chemical composition and sensory characteristics of two beef muscles (semimembranosus, SM and adductor, AD) from the inside round of Mexican and US origins. Inside rounds were obtained from 20 Mexican bull carcasses, representing beef of Mexican origin. Forty-vacuum packaged USDA Choice and Select inside rounds were purchased from a local trader, representing US beef. Muscles were analyzed for chemical composition, Warner-Bratzler shear force (WBSF), cooking loss percentage, instrumental color, textural profile, and consumer acceptability. Muscles imported from the US contained more intramuscular fat, had higher cooking loss percentages, lower shear force values, and higher consumer ratings for overall desirability than Mexican counterparts (P<0.05). Choice and Select beef samples had similar WBSF values (P>0.05). Consumers found differences in juiciness and tenderness ratings between the two muscles, with the SM being tougher and less juicy than the AD (P<0.05).     

不同烹饪方式的牛里脊质构品质比较

分析不同烹饪方式对黄牛牛里脊质构参数、脂肪酸含量的影响。购买新鲜黄牛牛里脊,分为5等份,分别做生牛里脊、炖制、烤制、油煎、真空低温烹饪。对比烹饪方式对黄牛牛里脊持水力、pH、粗蛋白、粗脂肪、感官品质、色泽、质构参数、脂肪酸含量的影响。真空低温牛里脊的持水力、pH值、粗蛋白、粗脂肪分别为89.98%、6.12、23.69 mg/g、23.69%。牛里脊色泽、嫩度、多汁性、风味评分分别为8.86、8.49、8.84、8.62分,色泽参数L^*值、a^*值、b^*值、c^*值、h0值分别为45.15、4.12、14.82、15.39、15.39;质构参数硬度、粘性、弹性、咀嚼性、内聚力、剪切力分别为8.36 N、0.62 N·s、6.98 mm、42.68 N·s、0.42 N/cm2、25.39 N;饱和脂肪酸含量、不饱和脂肪酸含量均较高。真空低温牛里脊与炖制牛里脊、烤制牛里脊、油煎牛里脊持水力、pH、粗蛋白、粗脂肪、感官品质、色泽、质构参数、脂肪酸含量相比,具有统计学差异(p<0.05)。由上述结果可知,不同烹饪的牛里脊与生牛里脊相比,存在一定的微观结构、质构变化和营养物质改变,真空低温烹饪方式下黄牛牛里脊微观结构较为完整,对质构的影响较小,更好的保留脂肪酸等营养成分,符合对黄牛牛里脊的烹饪要求。     

TEXTURE ANALYSIS OF BEEF COOKED AT VARIOUS TEMPERATURES BY MECHANICAL MEASUREMENTS, SENSORY ASSESSMENTS AND ELECTROMYOGRAPHY

The potential of mechanical measurements, sensory assessments and electromyography (EMG) to detect the variation in texture properties of beef induced by cooking at temperatures of 45–80C was determined. The mechanical behaviour of meat, from two Semimembranosus muscles from one animal, was studied by compression and shear tests in destructive and nondestructive conditions. Electromyographs from the masticatory muscles of 14 trained subjects were recorded during mastication of the meat samples. Texture profiles were completed at the end of each masticatory sequence. Increase in cooking temperature produced an overall increase in shear and compression parameters as well as in meat elasticity. Sensory hardness and total muscle work developed during mastication also increased with cooking temperature. Mechanical measurements, EMG parameters and sensory assessment of texture were highly correlated. EMG, on which the influence of psychological factors is limited, can be used to characterise meat texture objectively.     

Injection of marinade with actinidin increases tenderness of porcine M. biceps femoris and affects myofibrils and connective tissue

BACKGROUND: Marination of beef muscles with brine solutions containing proteolytic enzymes from fruit extracts has been shown to tenderize meat. However, the effect of marination with actinidin on tenderness of pork muscles has not been investigated. Tenderness and eating quality of porcine M. biceps femoris was investigated by Warner–Bratzler (WB) shear test and sensory evaluation after injection of brine containing up to 11 g L^?1 actinidin‐containing kiwi fruit powder and 2, 5 or 9 days of storage. RESULTS: Actinidin decreased WB shear force, increased tenderness and did not affect flavour and juiciness. Injection of 2.8 g L^?1 actinidin powder and storage for 2 days resulted in WB shear force values similar to control samples stored for 5 or 9 days. In samples injected with 10 g L^?1 actinidin powder, degradation of desmin and percentage of heat‐soluble collagen (P < 0.05) increased compared to control samples. Myofibrillar particle size tended to decrease (P < 0.1) with increasing actinidin concentration. No major changes were observed by proteome analysis. Atomic force microscopy showed actinidin‐induced damage of endomysium surrounding isolated single muscle fibres. CONCLUSION: Our results indicate that actinidin tenderizes pork M. biceps femoris by affecting both the myofibrils and connective tissue. Copyright © 2009 Society of Chemical Industry     

潮汕脆性肉丸的感官评定与质构评价相关性分析

目的:探索一种综合评价潮汕脆肉丸脆性品质的方法。方法:采用质构剖面法（texture profile analysis,TPA）和穿刺实验测定肉丸的质构特性,同时进行感官评定实验,并对感官和质构指标之间进行相关性分析。结果:TPA实验的硬度、咀嚼性与感官评定指标硬度、弹性、脆性、咀嚼性之间存在显著相关性（r=0.554~0.793,P<0.05）,TPA实验的回复性与感官评定的脆性、组织状态之间存在显著相关性（r=0.556,0.625,P<0.05）。穿刺实验的破裂力与感官评定指标之间均存在显著相关性（r=0.595~0.709,P<0.05）。运用主成分分析从仪器质构指标数据中提取出2个最主要的独立成分,这2个主成分的方差贡献率累计达74.5%。结论:潮汕肉丸的脆性可以采用TPA实验的硬度、咀嚼性和穿刺破裂力的定量评价结合感官评定进行综合评价,为构建客观、精确、便捷的潮汕脆性肉丸感官性状评价体系奠定基础。