Tribological interaction between multi-walled carbon nanotubes and silica surface using lateral force microscopy

This work presents the tribological interaction between multi-walled carbon nanotubes (MWCNTs) and silica surface using lateral manipulation in the atomic force microscope (AFM). The MWCNT is mechanically manipulated by a pyramidal silicon probe of an AFM using the same scan mechanism as in the imaging mode. With a controlled normal force of the AFM probe, it was found that lateral force applied to the MWCNT could overcome the tribological adhesion between MWCNT and silica surface, causing individual MWCNT to rotate on the silica. According to the results, the shear stresses due to tribological interacting with the MWCNTs and the silica are 59.6 MPa and 64.8 MPa for the MWCNT 1 (100 nm diameter) and the MWCNT 2 (60 nm diameter), respectively. Experimental results show that the shear stress increases with the increasing rotation angle for each manipulation, from which we determine the linear fitting function. In addition, we determine the relationship between push point and pivot point to realize the rotation behavior. The implications of tribological interaction between the MWCNTs and silica surface are discussed in detail.     

碳纳米管/金属复合材料的研究现状与动向

碳纳米管/金属复合材料具有优异的理化和力学性能。综述了碳纳米管金属基复合材料的国内外研究现状,分析了制约碳纳米管金属基复合材料广泛应用的因素,即碳纳米管的分散状态及其与金属基体的界面结合问题。最后,对制备碳纳米管金属基复合材料存在的问题及今后的研究动向进行了分析。     

Sinusoidal endothelial cells of the liver: Fine structure and function in relation to age

Liver endothelial cells form a continuous lining of the liver capillaries, or sinusoids, separating parenchymal cells and fat-storing cells from sinusoidal blood. Liver sinusoidal endothelial cells differ in fine structure from endothelial cells lining larger blood vessels and from other capillary endothelia in that they lack a distinct basement membrane and also contain open pores, or fenestrae, in the thin cytoplasmic projections which constitute the sinusoidal wall. This distinctive morphology supports the protective role played by liver endothelium, the cells forming a general barrier against pathogenic agents and serving as a selective sieve for substances passing from the blood to parenchymal and fat-storing cells, and vice versa. Sinusoidal endothelial cells, furthermore, significantly participate in the metabolic and clearance functions of the liver. They have been shown to be involved in the endocytosis and metabolism of a wide range of macromolecules, including glycoproteins, lipoproteins, extracellular matrix components, and inert colloids, establishing endothelial cells as a vital link in the complex network of cellular interactions and cooperation in the liver. Fine structural studies in combination with the development of cell isolation and culture techniques from both experimental animal and human liver have greatly contributed to the elucidation of these endothelial cell functions. Morphological and biochemical investigations have both revealed little changes with age except for an accumulation of iron ferritin and a decrease in the activities of glucose-6-phosphatase, Mg-ATPase, and in glucagon-stimulated adenylcyclase. Future studies are likely to disclose more fully the role of sinusoidal endothelial cells in the regulation of liver hemodynamics, in liver metabolism and blood clearance, in the maintenance of hepatic structure, in the pathogenesis of various liver diseases, and in the aging process in the liver.     

Stereological estimation of average cell volume in monolayer culture by combined light and electron microscopy

In order to convert stereological ratio estimates into ‘absolute’ values (‘per cell’ values) the average cell volume must be estimated. The present paper describes a stereological method based on well-known point counting procedures for the estimation of average cell volumes in monolayer cultures fixed in situ. This method involves estimation of the average attachment area per cell by light microscopy combined with estimation of the attachment membrane surface density by electron microscopy. There is no need for any assumption as to cellular or nuclear shape. The method has been tested on an established cell line, NHIK 3025, and shows a good accuracy. It has also been used to analyse the volume changes that take place in human monocytes during monolayer culture, demonstrating a 28-fold increase of the average cell volume over 10 days.     

Microscopy of cell cultures through thin plastic films

Microscopy of cells growing in vessels containing plastic films as a substrate or as a transparent window is facilitated by a contact cap on the objective or the contact objectives for intravital microscopy. When applied to microscopical examination of living cells through a thin film, the cap considerably improves the conditions of observation with high-power dry objectives and makes it possible to use water- and oil-immersion objectives.     

Preparation and tribological properties of the carbon nanotubes–Ni–P composite coating

A method to prepare the carbon nanotubes (CNTs)–Ni–P composite coating with different mass content of CNTs on the surface of 45^# steel by electroless plating was proposed. The transmission electron microscopy (TEM) and the scanning electron microscopy (SEM) were used to observe the appearance of the as-prepared CNTs and the CNTs–Ni–P composite coating, and then the roughness of the coating surface was also analyzed by atomic force microscopy (AFM). Furthermore, the wear and friction behavior of the CNTs–Ni–P composite coating were investigated under oil-lubricated condition, Due to the self-lubrication property and the unique antifriction structure, CNTs can greatly improve the wear resistance of the CNTs–Ni–P composite coating, where the wear resistance of the CNTs–Ni–P composite coating is optimized with the intermediate mass content of 2 kg/m³ CNTs.     

Nanotechnology and nanostructured materials: trends in carbon nanotubes

Carbon nanotubes have attracted the attention of many researchers since their discovery last decade. These carbon molecules are tiny tubes with diameters down to 0.4 nm, while their lengths can grow up to a million times their diameter. Using their remarkable electrical properties, simple electronic logic circuits have been built. These structures are promising for the semiconductor industry which is leading the search for miniaturisation. They are not only very good conductors, but they also appear to be the yet found material with the biggest specific stiffness, having half the density of aluminium. This paper is written to give a consolidated view of the synthesis, the properties and applications of carbon nanotubes, with the aim of drawing attention to useful available information and to enhancing interest in this new highly advanced technological field for the researcher and the manufacturing engineer.     

Interaction forces and conduction properties between multi wall carbon nanotube tips and Au(111)

We have studied the interaction forces and electrical conduction properties arising between multiwall carbon nanotube tips and the Au(111) surface in air, by means of amplitude modulation scanning force microscopy, also called intermittent contact. We have centered our work on tips with metallic electronic structure and for the specific parameters used we have found a preliminary interaction range where there is no contact between tip and surface. Stable imaging in this non-contact range is possible with multiwall carbon nanotube tips. These tips have also been used to obtain simultaneous topographic and current maps of the surface. They show excellent properties as tips due to their high aspect ratio and durability, as a result of their elastic and non-reactive properties. Correspondingly, multiwall carbon nanotube tips allow high resolution local analysis of electrical conductivity on a nanometer scale.     

Generation of spatio-temporal concentration profiles for cell culture systems: A case study in ammonia

Cellular behavior is influenced by micro-concentration gradients existing in their immediate spatio-environment. A practical real-time measurement system, composed of miniaturized long-lasting sensors placed within a two-dimensional bioreactor and a 32-channel data acquisition system for ammonia, is presented in this paper aiming to generate the spatio-temporal ammonia concentration profiles for the whole culture process. Our results demonstrate the feasibility of the system and its applicability in elucidating the cell growth micro-environment within a bioreactor, which can potentially facilitate the intelligent control of ammonia levels in cell culture systems. The presented system is flexible enough to interface other sensors for generating their spatio-temporal profiles of the sensed cell culture parameters.     

A tradeoff analysis between process plan selection and cell formation in cellular manufacturing

In this paper, an algorithm to evaluate the tradeoff between conflicting objectives in process plan selection and cell formation is developed. Consideration of the minimisation of intercell material movement in cellular manufacturing is necessary but not in itself sufficient to produce a system for which the total work content is minimised. Solving the process plan selection and the cell formation problem for all possible alternative process plans is a time-consuming task, and therefore not economically justifiable. The algorithm is illustrated through the use of a sample problem that shows how it is possible to create a cell using the algorithm presented in this paper.     

Microenvironment and cell mechanics

Microenvironment contains biophysical and biochemical elements to maintain survival, growth, proliferation,and differentiation of cells. Any change can lead to cell response to the mechanical forces, which can be described byelasticity. It is an indicator of a cell’s state since it plays an important role in many cellular processes. In many cases,cell elasticity is measured by using discontinuous manner, which may not allow elucidating real-time activity ofindividual live cells in physiological condition or cell response against microenvironmental changes. I argue thatmeasuring cell elasticity using continuously repetitive nanoindentation technique is important that should beconsidered. As an example, I discuss mechanics of human embryonic kidney (HEK) cells in various conditions. Inresting cells, there is an activity of the cytoskeleton whose oscillation amplitude is strongly affected by the intracellularcalcium, and the collective activity of myosin motor proteins induces elasticity oscillation. Experimental results alsoreveal that actin cytoskeleton and cell membrane determine cell mechanics.     

Solid lubrication by multiwalled carbon nanotubes in air and in vacuum

Aligned and dispersed multiwalled carbon nanotubes (MWNTs) were evaluated for solid lubrication applications. Results obtained from unidirectional sliding friction experiments indicate that MWNTs have superior friction properties and sustainability in air and vacuum. Based on analyses of wear surfaces, transfer films, wear debris, and microstructures, the lubrication mechanism of the MWNTs is discussed.†NASA Resident Research Associate at Glenn Research Center     

Friction and dry sliding wear of bismaleimide filled with carbon nanotubes

Three types of bismaleimide–carbon nanotubes (CNTs) nanocomposites were fabricated using two types of original multiwalled CNTs with different diameters and one amide functionalized CNTs. The influence of diameter, content and functionalization of CNTs on the flexural and dry sliding wear behaviour were measured with universal testing machine and pin-on-disc wear apparatus. The experimental results indicated that at 1.5 wt-%, the bismaleimide-functionalized MWCNTs exhibited highest flexural strength of 156 MPa which is increased by 164% as compared to the neat matrix, and lowest specific wear rate of 1.8 × 10^?4 mm³ N^?1 m^?1 which is decreased by 90% as compared to the neat matrix. This was attributed to the dispersion of CNTs in the matrix and the filler-matrix adhesion and internal strength of the composite.     

An easy method for orientated embedding tissue culture cell monolayers in LR white resin for postembedding immunocytochemistry

Partial polymerization of LR White resin blocks is a frequently encountered problem if oxygen is present during the polymerization reaction. Instructions are given for a simple method to embed cell culture monolayer in LR White acrylic resin, which is suitable for immunocytochemistry. The advantage of this method is that it is easy and reliable. It also ensures accurate orientation of the cell monolayer in relation to the desired plane of sectioning.     

Interaction between zinc dialkyldithiophosphate and amine

This paper reports work on complex formation from ZDP and various aliphatic amines, and their equilibrium states in oil, adsorptivities and antiwear properties are investigated. Complex formation from ZDP and amine was proven by isolation and identification of solid crystals, and various complexes were found from mono- and diamines. The complex from monoamine has lower adsorptivity than free ZDP, while the poorer adsorptivity of ZDP/monoamine complex makes its antiwear property worse.     

Carbon nanotubes and other graphitic structures as contaminants on evaporated carbon films

Evaporated carbon support films for transmission electron microscopy (TEM) often contain fullerene-like structures such as nanotubes and nanoparticles. This could cause serious confusion in TEM studies of fullerene-related carbons. Other, poorly graphitized, particles are also frequently seen.     

Concurrent design of manufacturing cell and control functions: A neural network approach

This paper presents a neural network based decision support system (DSS) for use in concurrently determining cell configuration, operation plans, and complexity requirements of cell control functions. Advanced simulators and neural network technology are used in developing the DSS. Simulation experiments were conducted with many possible combinations of design changes to generate training pairs for a neural network. Complexity of cell control functions required by each design option was assessed, based on operational requirements, and was used to train another neural net. Once both neural networks are properly trained, one network can be used to predict the cell design configuration given a set of desirable cell performance measures, while the other network can be used to identify complexity requirements of the cell control functions by using the output provided by the first network as input to the second neural net. An operation-driven cell design methodology was applied to sequentially predict requirements of both cell configuration and cell control functions from the trained neural networks. This innovative new design methodology was illustrated via a successful implementation exercise in acquiring a real automated manufacturing cell at industrial settings. The exercise proves that such a DSS serves well as an effective tool for cell designers and the management in determining appropriate cell configuration and cell control functions at the design stage.     

几种贝类组织细胞培养的研究

贝类组织细胞培养研究始于上世纪六十年代。相对于建系技术已很成熟的哺乳动物和昆虫,贝类细胞培养的发展一直处于落后状态。现综述几种作为重要海产养殖品种的贝类组织细胞培养的研究,并总结目前在该领域面临的问题,以期为摸索贝类细胞培养条件、进一步建立起细胞系提供参考。     

Preparation of collagen gel matrices for light and electron microscopy

Cells grown on type I hydrated collagen gels require special techniques for sample preparation and processing in order to optimize the removal of all reagents from the collagen matrix and prevent artifactual shrinkage. This method includes cutting out a small block of the collagen gel, postfixation, and transfer to a scintillation vial for further processing. These additional steps ensure that all sides of the block will come in contact with solutions and reduces the possibility of reagent trapping within the collagen matrix. Additionally, in our study the collagen gel and endothelial cells that form a monolayer on the surface are oriented to allow the microtomist greater assurance of cutting true cross sections, thus saving time and increasing reproducibility. The dehydration sequence is also modified, with an increase in the times and additional steps, especially in the higher concentrations of dehydrant.     

Tribological behavior and graphitization of carbon nanotubes grown on 440C stainless steel

Vertically aligned carbon nanotube (CNT) arrays were directly grown onto 440C stainless steel substrates by plasma-enhanced chemical vapor deposition. Tribological properties of both short and long CNTs samples were studied under normal loads of 10 g, 25 g and 100 g. The CNTs had a steady-state friction coefficient of about 0.2 in humid air. In dry nitrogen, a friction of 0.2 was measured under a load of 10 g while high friction was measured at 25 g and 100 g loads. No significant variation of tribological behavior was measured between the short and long CNTs samples. SEM observations showed that rubbing caused the CNTs to align or lay down along the wear scar. They formed aggregates and were compressed by rubbing, which resulted in layer-structured graphite formations. SEM observation of the wear scars revealed loss of CNT structures accompanied by the appearance of dark areas. Micro Raman spectroscopic studies demonstrated that the dark areas were graphitized CNTs. Shear stress aligned the basal planes of the small graphene sheets in the CNT layers to the low friction orientation and eventually caused formation of more ordered graphite. The tribological formation of interfacial carbon layers increased with increasing stress from higher loads.