共查询到19条相似文献,搜索用时 109 毫秒
1.
2.
3.
大豆粗磷脂中磷脂酰胆碱的分离纯化研究 总被引:2,自引:0,他引:2
为了筛选出从醇溶性大豆磷脂中分离纯化大豆磷脂酰胆碱的最佳分离纯化工艺,本文了采用正交设计试验筛选提取大豆磷脂酰胆碱的最佳工艺。结果表明大豆磷脂酰胆碱最佳提取工艺为:按1∶10的料水比进行加热,在78℃恒温下4 h回流提取,重复2次。大豆磷脂酰胆碱的进一步的精制方法为葡聚糖凝胶Sephadex LH-20凝胶柱层析法,并且考察了葡聚糖凝胶Sephadex LH-20对大豆磷脂酰胆碱的纯化能力。首先采用无水乙醇作为葡聚糖凝胶Sephadex LH-20凝胶柱层析洗脱液纯化大豆磷脂酰胆碱,然后将所得的大豆磷脂酰胆碱干燥,最后通过高效液相色谱法对所得的大豆磷脂酰胆碱进行含量测定。结果表明,乙醇提取法以及葡聚糖凝胶Sephadex LH-20柱层析法相结合的纯化过程对大豆磷脂酰胆碱分离纯化效果良好,此外该方法收率高,能够得到纯度为90.12%的大豆磷脂酰胆碱。 相似文献
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
Abdelmoneim H. Ali Xiaoqiang Zou Sherif M. Abed Sameh A. Korma Qingzhe Jin 《Critical reviews in food science and nutrition》2019,59(2):253-275
During the last years, phospholipids (PLs) have attracted great attention because of their crucial roles in providing nutritional values, technological and medical applications. There are considerable proofs that PLs have unique nutritional benefits on human health, such as reducing cholesterol absorption, improving liver functions, and decreasing the risk of cardiovascular diseases. PLs are the main structural lipid components of cell and organelle membranes in all living organisms, and therefore, they occur in all organisms and the derived food products. PLs are distinguished by the presence of a hydrophilic head and a hydrophobic tail, consequently they possess amphiphilic features. Due to their unique characteristics, the extraction, separation, and identification of PLs are critical issues to be concerned. This review is focused on the content of PLs classes in several sources (including milk, vegetable oils, egg yolk, and mitochondria). As well, it highlights PLs biosynthesis, and the methodologies applied for PLs extraction and separation, such as solvent extraction and solid-phase extraction. In addition, the determination and quantification of PLs classes by using thin layer chromatography, high-performance liquid chromatography coupled with different detectors, and nuclear magnetic resonance spectroscopy techniques. 相似文献
16.
近年来,食源性生物活性肽在功能食品中的应用越来越广,从而使其成为食品领域研究的热点问题。本文对酶解法、微生物发酵法及人工合成法制备食源性性生物活性肽进行简单介绍,根据食源性活性肽分子量及其所带电荷,对食源性活性肽的分离纯化进行重点概述。 相似文献
17.
18.