共查询到17条相似文献,搜索用时 140 毫秒
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实验将一种阴离子表面活性剂(AOT)和一种阳离子表面活性剂(Aliquat336)分别溶于异辛烷(isooctane)中,构成了两种不同的反胶团体系.通过用两种不同的反胶团溶液萃取六种蛋白质的实验,研究了水相pH值及离子强度对反胶团体系中水含量Wo和蛋白质萃取率影响的规律.实验发现不论哪种反胶团体系,水相pH值对Wo的影响都不大,但pH值却对蛋白质萃取率有很大的影响,对AOT反胶团体系而言,随着pH值的降低,蛋白质的萃取率升高;对Aliquat336反胶团体系而言,随着水相pH值的降低,蛋白质的萃取率下降.随着离子强度的增大,AOT反胶团体系的水含量大幅度地降低;而对Aliquat336体系水含量的影响很小,但水相离子强度对蛋白质萃取率的影响是相同的,随着离子强度的增大,两种体系对蛋白质的萃取率均下降。 相似文献
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反胶团酶催化研究新进展 总被引:3,自引:0,他引:3
评述了近年来反胶团酶催化研究的新进展。在AOT/异辛烷反胶团中加入非离子型表面活性剂如Tween 85、小相对分子质量聚乙二醇等可有效降低酶与表面活性剂间的静电和疏水作用 ,显著提高酶的活性。对AOT进行化学修饰及合成结构与磷脂类似的新表面活性剂以用其构建新的反胶团体系 ,酶的活性较常用的AOT/异辛烷反胶团体系有显著提高。在反胶团酶反应动力学研究中考虑水含量或底物在反胶团表面吸附的影响等 ,提出了进一步研究的设想 ,包括开发新型表面活性剂以进一步提高酶的活性和稳定性及有利于产物分离 相似文献
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利用超临界流体CO2反胶团-络合萃取食品中痕量重金属铅、汞和砷,通过正交实验考察了萃取压力、萃取温度、萃取时间和反胶团AOT浓度对痕量重金属萃取率的影响。确定最佳萃取工艺为:萃取压力20 MPa、萃取温度45℃、萃取时间90 min、反胶团AOT浓度0.1 mol.L-1,此时,重金属铅、汞、砷的萃取率分别为93.50%、95.36%、90.47%。表明超临界流体CO2络合萃取时加入反胶团能明显提高痕量重金属的脱除率,萃取效果优于超临界流体CO2反胶团萃取和超临界流体CO2络合萃取。 相似文献
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研究了超临界CO2下AOT/CTAB混合反胶团体系萃取乳酸钙中影响产品品质的杂蛋白质.并且分析了萃取压力P、温度T、AOT用量VAOT及 AOT/CTAB摩尔混合比nAOT / nCTAB、水量VH2O、pH值等各因素对于该体系中蛋白质的萃取率的影响.通过正交试验得出最佳操作条件 P = 25 MPa,T = 45℃,VAOT =0.15 mL·(g乳酸钙)-1,nAOT/nCTAB = 3001, VH2O = 1.6mL水·(g乳酸钙)-1,pH =4.在此操作条件下,蛋白质的去除率可达70%. 相似文献
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研究了二(2-乙基己基)琥珀酸酯磺酸钠(AOT)和四聚氧乙烯基苯壬基酚醚(PNE4)混合表面活性剂反胶团系统萃取溶菌酶的平衡行为,建立了考虑静电作用和疏水性作用贡献的反胶团萃取蛋白质的分配平衡模型,实验验证了模型的可靠性和适用性. 研究发现,有Na+存在时AOT的疏水性较K+存在时小,而PNE4的疏水性因子与盐离子种类无关;Na+对溶菌酶表面疏水性的增大效应较K+显著;K+在反胶团内界面上的吸附作用大于Na+. 利用静电学和热力学理论讨论和解释了有关现象. 相似文献
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Macroporous poly(styrene-divinyl benzene) microspheres with pore size of about 500 nm were prepared by a new method, surfactant reverse micelles swelling method. The macroporous microspheres were prepared by convenient suspension polymerization. The difference from conventional suspension polymerization was that a higher concentration of surfactant was added in the oil phase. The effects of the amount and type of surfactants on the morphology of microspheres were investigated, and the formation mechanism was also discussed. Macropores were formed when the concentration of surfactant was much higher than critical micelle concentration (cmc). It was proposed that a large amount of reverse micelles formed by adding a large amount of surfactant in the oil droplet phase, and the reverse micelles could absorb water from the external aqueous phase. The water in the oil phase formed macropores after polymerization. The method developed in this study was convenient to prepare microspheres with larger pore size than the conventional method such as agglomeration method of nanoparticles. 相似文献
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XRD,SEM, and XPS Analysis of Soybean Protein Powders Obtained Through Extraction Involving Reverse Micelles
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![点击此处可从《Journal of the American Oil Chemists](/ch/ext_images/free.gif)
Xiaoyan Zhao Haitao Zhu Bingwen Zhang Jun Chen Qiang Ao Xiyuan Wang 《Journal of the American Oil Chemists' Society》2015,92(7):975-983
Soybean protein powders obtained by aqueous buffer and reverse micelle extractions were examined and characterized using X‐ray photoelectron spectroscopy (XPS), X‐ray diffraction (XRD), and scanning electron microscopy (SEM). These analysis methods provided detailed information about elemental distributions, surface structure, and secondary and microstructures of the protein, respectively. XPS data revealed that the O and N atomic percentages of soybean protein surfaces obtained with bis(2‐ethylhexyl) sodium sulfosuccinate (AOT)/hexane reverse micelles were higher than those obtained with aqueous buffer, whereas the percentage of atomic C was lower. The ratios of nitrogen to carbon (N/C) on the surface of soybean protein obtained through the two extraction methods were similar. The O/C ratio for soybean protein obtained from AOT reverse micelles was large. The obtained results indicated that the reverse micelles could affect the C, O, and N components on the surface of soybean proteins. Moreover, XRD and SEM results also showed the influence of AOT reverse micelles, which lead to more β‐sheet and pore structures. 相似文献
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《分离科学与技术》2012,47(12-13):2015-2030
Abstract Initial results are presented showing the potential applications of reverse micelle supercritical fluid solvents in separation processes. The formation of reverse micelles in supercritical nalkane continuous phases is described. Phase diagrams obtained from view-cell studies of micellar and microemulsion phases formed in supercritical fluids are reported and shown to be strongly dependent on pressure. The solubility of AOT in ethane and propane over a range of pressures shows behavior typical of solids in supercritical fluids. The maximum water-to-surfactant ratio (WO) increased dramatically in both ethane and propane systems as pressure was increased. At 300 bar WO = 4 for ethane at 37 'C and WO = 12 for propane at 103 *C. The initial use of supercritical fluids containing reverse micelles for the extraction of solutes from an aqueous phase, and as mobile phases in chromatography, is described. 相似文献
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Rangaswamy Subramanian Sosaku Ichikawa Mitsutoshi Nakajima Toshinori Kimura Takaaki Maekawa 《European Journal of Lipid Science and Technology》2001,103(2):93-97
Studies were conducted to determine the critical micelle concentration (CMC) of phospholipids in vegetable oils and the size of reverse micelles to understand their rejection phenomenon in the membrane process. The CMC values of phosphatidylcholine (PC) in triolein and phospholipids in crude soybean oil were determined to be 440 and 1020 mg/kg, respectively, by using TCNQ (7,7,8,8‐tetracyano‐quinodimethane) solubilization technique. The surface tension measurements of these samples gave similar values of CMC. From small‐angle X‐ray scattering (SAXS) analysis, the size of the PC micelles was determined to be in the range of 3.56 to 4.70 nm. The characterization of reverse micelles formed in the oil system was found useful in enhancing the understanding of the possible rejection phenomenon of phospholipids by non‐porous polymeric composite membranes used in our earlier studies on vegetable oils and in suggesting suitable types of membranes for the same. 相似文献
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Effects of AOT reverse micelle extraction on structure and emulsifying properties of soybean protein
Lifen Zhang Mingzhu Zhang Xiaoyang Sun Fusheng Chen Qian Wu 《Journal of the American Oil Chemists' Society》2021,98(9):923-932
The present study aimed to investigate the forward and backward extraction efficiency, structure, and emulsifying properties of soybean proteins obtained through bis (2-ethylhexyl) sulfosuccinate sodium salt (AOT) reverse micelle, as well as their relationship. The results showed that the extraction efficiency was the highest with the forward extraction time of 25 min, pH of 3.0, temperature of 45°C, KCl concentration of 0.0 mol L−1, and the backward extraction time of 10 min, pH of 4.0, temperature of 30°C, KCl concentration of 0.5 mol L−1. Significant changes in the secondary structure and fluorescence intensity of soybean protein after AOT reverse micelle extraction were observed. More β-sheet structure and higher fluorescence intensity corresponded to the higher emulsion stability of soybean protein. The results indicated that reverse micelle extraction shows great application in food industry. 相似文献