双歧杆菌酸奶制造的中间试验

应用筛选获得的双歧杆菌耐氧菌株发酵制造酸牛奶的工艺进行了中间试验。结果表明:采用双歧杆菌耐氧菌株进行有氧奶质发酵制造酸牛奶是完全可行的。适当添加一些生长促进物质,可使发酵剂制备时间减少到8小时以内。采用由双歧杆菌和其它辅助菌混合而成的组合发酵剂制造酸奶,发酵周期可缩短到5小时;此种酸奶在保质期内双歧杆菌活菌数含量能保持在10~7个/g以上。应用此工艺,只需采用普通酸奶的生产设备即可进行双歧杆菌酸奶的制造。     

双岐杆菌酸奶的研制

就双歧杆菌以脱脂牛乳为培养基的耐氧发酵条件进行了探索,试验了导致双歧杆菌耐氧增殖的几种促进剂,且获得产菌量较高的发酵培养基,并将此菌应用于发酵乳制品。经试验确定了最佳发酵工艺,使用BAT混合菌种,或者将婴儿双歧杆菌、嗜酸乳杆菌、嗜热链球菌分别单独培养后按不同比例混合培养制备工作发酵剂,然后就可在常规条件下生产出双歧杆菌、嗜酸乳杆菌的活菌个数均高达10~(7~8)/ml以上、风味与普通酸乳相似、营养保健价值远高于普通酸乳的双歧杆菌酸奶。     

降胆固醇双歧杆菌BZ11的筛选及其耐受性评价

采用平板涂布法从贵州特色香猪粪便中筛选具有降胆固醇能力及耐酸、耐胆盐和耐氧性能的双歧杆菌（Bifidobacterium）。从香猪粪便中共筛选出19株具有双歧杆菌形态特征的菌株。采用邻苯二甲醛法测定其降胆固醇率,得到7株降胆固醇能力较高菌株,其中菌株BZ11降胆固醇率最高可达（38.52±0.60）%,并对酸、胆盐和氧具有较高的耐受性。经菌落形态特征、生理生化试验及16S rRNA序列分析,菌株BZ11被鉴定为动物双歧杆菌乳亚种（Bifidobacterium animalis subsp. lactis）。     

两歧双歧杆菌耐氧耐酸优良菌株的选育

两歧双歧杆菌通过逐渐增加培养基中氧气分压的方法进行多次耐氧驯化,每次耐氧驯化后进行挑选耐氧的两歧双歧杆菌单菌落,然后将经过耐氧驯化的两歧双歧杆菌菌株再通过降低培养基pH值的方法来进行多次耐酸驯化,每次耐酸驯化后进行挑选两歧双歧杆菌单菌落,以确定所获得的两歧双歧杆菌为纯的耐酸菌株.最终得到了1株对氧和酸的耐受能力有较大提高的两歧双歧杆菌菌株,且耐氧耐酸驯化后的菌株没有发生变异.这有利于两歧双歧杆菌产品的开发和保持两歧双岐杆菌制品有较高的活菌量,具有较好的实际应用价值.     

双歧杆菌发酵酸豆奶的研制

就双歧杆菌以豆奶为培养基的耐氧条件进行了探索,经实验确定了发酵工艺,制作的双歧杆菌发酵酸豆奶具有普通发酵酸豆奶的典型风味,还含有双歧杆菌活菌达１０８ 以上。     

双歧杆菌发酵中国黑米口服液的研制

以中国黑米为主要原料，制作发酵培养基，采用耐氧两歧双歧杆菌进行发酵扩大培养，以双歧杆菌发酵中国黑米口服液。应用该口服液对腹泻、消化不良病人进行治疗观察，疗效明显     

保健食品—BB乳的研究

本文就双歧杆菌耐氧型菌株的生物学特性及采用该菌株经牛奶发酵而制成的双歧杆菌活菌制剂——BB乳的工艺条件进行了探讨和研究。通过筛选和驯化所得到的耐氧型菌株与出发菌株相比,耐氧特性明显提高,能更好适合生产的需要。所研制的活菌制剂BB乳对主要常见的肠道致病菌有较好的杀灭作用,是一种很好的保健食品。     

青春双歧杆菌的耐氧驯化及不同低聚糖对其增殖效果的影响

为改善青春双歧杆菌在发酵产品中活菌数低、菌种功效弱的情况,对厌氧青春双歧杆菌进行耐氧驯化,采用无氧有氧交替驯化法,在驯化过程中逐渐增加青春双歧杆菌培养液的氧分压,测定青春双歧杆菌驯化前后生理特性,并对比大豆低聚糖、低聚木糖、低聚麦芽糖对青春双歧杆菌增殖的影响。结果表明,青春双歧杆菌在耐氧驯化后对氧气的敏感程度下降,有氧条件下生长能力达标,产酸能力得到提升,菌体驯化前后形态基本一致,耐氧青春双歧杆菌代谢产生的乙酸与乳酸比值较厌氧青春双歧杆菌更适宜发酵,在3种低聚糖中,低聚木糖对青春双歧杆菌增殖作用明显,可作为青春双歧杆菌良好的双歧因子。耐氧驯化后的青春双歧杆菌有着优良的生理特性,能够作为潜在的益生菌菌种深入研究其功能并可应用于发酵食品中。     

降胆固醇合生元组合的体外筛选

为了筛选出对心脑血管病具有预防保健作用的降胆固醇合生元组合,本研究利用体外法对水苏糖、低聚异麦芽糖、低聚果糖、山梨醇、水解酪蛋白5种益生元和保加利亚乳杆菌、婴儿双歧杆菌2株益生菌进行了降胆固醇合生元组合筛选。结果表明:不同组合去除胆固醇的能力不同,去除率在2.1%～43.2%之间,其中保加利亚乳杆菌在添加山梨醇的培养基中降低胆固醇效果显著(P<0.05),去除率达43.2%;婴儿双歧杆菌在添加山梨醇和酪蛋白的混合益生元培养基中降低胆固醇效果显著(P<0.05),去除率为27.0%。     

SOD双歧酸奶的研制

对双歧杆菌进行耐氧驯化、发酵制造双歧杆菌酸奶是可行的，双歧杆菌和酸奶菌种单独接种发酵，并按１：２比例混合发酵乳，产品可获得较高的双歧杆菌活菌数１０＾８／ｍｌ以上，和良好的酸奶风味，双歧杆菌单独发酵牛乳时，８ｈ是较理想的发酵终点，发酵后在酸奶中添加ＳＯＤ可保持酸奶中较高的ＳＯＤ活性，ＳＯＤ双歧杆菌酸奶在４￣６℃保存２０天，ｐＨ４．１，ＳＯＤ活性可保持７０％以上。     

Reliable identification of grapevine rootstock varieties using RAPD PCR on woody samples

Since grapevine ( Vitis spp .) rootstock material is being traded increasingly as disbudded woody material a lack of distinctive morphological features on such material necessitates an alternative and reliable means of identification. Methods described here were developed for rapid and efficient extraction of DNA from woody samples rich in phenolic compounds and polysaccharides, and for subsequent identification of varieties by RAPD PCR. Using these methods, and with the application of only one selected RAPD primer, we were able to differentiate sixteen rootstock varieties, including the seven varieties most commonly used in Germany. Problems commonly encountered with reproducibility of RAPD patterns were avoided by choosing primers with a dinucleotide sequence and a high G/C content that allowed a rather high annealing temperature of 45°C. Methods described here should also be useful for other horticultural crops, especially those with woody tissues rich in phenolic compounds and polysaccharides.     

An internet compendium of analytical methods and spectroscopic information for monomers and additives used in food packaging plastics

An internet website (http://cpf.jrc.it/smt/) has been produced as a means of dissemination of methods of analysis and supporting spectroscopic information on monomers and additives used for food contact materials (principally packaging). The site which is aimed primarily at assisting food control laboratories in the European Union contains analytical information on monomers, starting substances and additives used in the manufacture of plastics materials. A searchable index is provided giving PM and CAS numbers for each of 255 substances. For each substance a data sheet gives regulatory information, chemical structures, physico-chemical information and background information on the use of the substance in particular plastics, and the food packaging applications. For monomers and starting substances (155 compounds) the infra-red and mass spectra are provided, and for additives (100 compounds); additionally proton NMR are available for about 50% of the entries. Where analytical methods have been developed for determining these substances as residual amounts in plastics or as trace amounts in food simulants these methods are also on the website. All information is provided in portable document file (PDF) format which means that high quality copies can be readily printed, using freely available Adobe Acrobat Reader software. The website will in future be maintained and up-dated by the European Commission's Joint Research Centre (JRC) as new substances are authorized for use by the European Commission (DG-ENTR formerly DGIII). Where analytical laboratories (food control or other) require reference substances these can be obtained free-ofcharge from a reference collection housed at the JRC and maintained in conjunction with this website compendium.     

Aroma characterization of various apricot varieties using headspace–solid phase microextraction combined with gas chromatography–mass spectrometry and gas chromatography–olfactometry

The characterization of the aromatic profile of several apricot cultivars with molecular tracers in order to obtain objective data concerning the aromatic quality of this fruit was undertaken using headspace–solid phase microextraction (HS–SPME). Six apricot cultivars were selected according to their organoleptic characteristics: Iranien, Orangered, Goldrich, Hargrand, Rouge du Roussillon and A4025. The aromatic intensity of these varieties measured by HS–SPME–Olfactometry were defined and classified according to the presence and the intensity of grassy, fruity and apricot like notes. In the six varieties, 23 common volatile compounds were identified by HS–SPME–GC–MS. Finally, 10 compounds, ethyl acetate, hexyl acetate, limonene, β-cyclocitral, γ-decalactone, 6-methyl-5-hepten-2-one, linalool, β-ionone, menthone and (E)-hexen-2-al were recognized by HS–SPME–GC–O as responsible of the aromatic notes involved in apricot aroma and considered as molecular tracers of apricot aromatic quality which could be utilized to discriminate apricot varieties.     

Tests of potential functional barriers for laminated multilayer food packages. Part I: Low molecular weight permeants

The advent of the functional barrier concept in food packaging has brought with it a requirement for fast tests of permeation through potential barrier materials. In such tests it would be convenient for both foodstuffs and materials below the functional barrier (sub-barrier materials) to be represented by standard simulants. By means of inverse gas chromatography, liquid paraffin spiked with appropriate permeants was considered as a potential simulant of sub-barrier materials based on polypropylene (PP) or similar polyolefins. Experiments were performed to characterize the kinetics of the permeation of low molecular weight model permeants (octene, toluene and isopropanol) from liquid paraffin, through a surrogate potential functional barrier (25 μm-thick oriented PP) into the food simulants olive oil and 3% (w/v) acetic acid. These permeation results were interpreted in terms of three permeation kinetic models regarding the solubility of a particular model permeant in the post-barrier medium (i.e. the food simulant). The results obtained justify the development and evaluation of liquid sub-barrier simulants that would allow flexible yet rigorous testing of new laminated multilayer packaging materials.     

Analysis of benzo[a]pyrene in spiked fatty foods by second derivative synchronous spectrofluorimetry after microwave-assisted treatment of samples

A simple, rapid and inexpensive method has been developed for the determination of benzo[a     

The development of reference materials for paralytic shellfish poisoning toxins in lyophilized mussel. II: Certification study

This paper describes the second part of a project undertaken to develop certified mussel reference materials for paralytic shellfish poisoning toxins. In the first part two interlaboratory studies were undertaken to investigate the performance of the analytical methodology for several PSP toxins, in particular saxitoxin and decarbamoyl-saxitoxin in lyophilized mussels, and to set criteria for the acceptance of results to be applied during the certification exercise. Fifteen laboratories participated in this certification study and were asked to measure saxitoxin and decarbamoyl-saxitoxin in rehydrated lyophilized mussel material and in a saxitoxin-enriched mussel material. The participants were allowed to use a method of their choice but with an extraction procedure to be strictly followed. The study included extra experiments to verify the detection limits for both saxitoxin and decarbamoyl-saxitoxin. Most participants (13 of 15) were able to meet all the criteria set for the certification study. Results for saxitoxin.2HCl yielded a certified mass fraction of <0.07 mg/kg in the rehydrated lyophilized mussels. Results obtained for decarbamoyl-saxitoxin.2HCl yielded a certified mass fraction of 1.59+/-0.20 mg/kg. The results for saxitoxin.2HCl in enriched blank mussel yielded a certified mass fraction of 0.48 +/- 0.06 mg/kg. These certified reference materials for paralytic shellfish poisoning toxins in lyophilized mussel material are the first available for laboratories to test their method for accuracy and performance.     

Announcing a new international peer-reviewed journal:Food Science and Human Wellness now accepting manuscript submission in English

<正>We are pleased to announce the launch of a new international peer-reviewed journal-Food Science and Human Wellness,ISSN 2213-4530,which is an open access journal,produced and hosted by Elsevier B.V.on behalf of Beijing Academy of Food Sciences.Food Science and Human Wellness is an international peer-reviewed English journal that provides a forum for the dissemination of the     

Chinese CTP Market

According to Printing and Printing Equipment Industries Association of China（PEIAC）＇s statistics to the plate manufucturer in China, in 2013, the actual offset plate production has reached 346 million square meters in China. Among them, the CTP production volume was 245 million square meters, up by 11% than that of last year; the total sales of the CTP plate was 239 million square meters, up by 13%.     

Preparatory Work of Print China 2015 is Going Smoothly

正Held at Guangdong Modern International Exhibition Center,Print China 2015 will cover 7exhibition halls,besides the original Hall No.3,4,5,6,7,the newly built F zone of Hall 3 will be used too.The total area will be140,000 square meters.Hall 3:Offset and large printing equipment,package printing equipment,post press