Instrumental response persistence following induction of hippocampal theta frequency during fixed-ratio responding in rats.

Four groups of 6 male albino rats (24 Ss) were used to investigate the relationship between hippocampal theta and instrumental response persistence. All Ss were first trained on a discrete-trial FR-10 schedule of reinforcement. In Phase 2, hippocampal theta was induced via electrical stimulation of medial septal pacemaker cells through implanted electrodes at the beginning of 50% of the 8 daily FR-10 trials for experimental Ss, while stimulated-control Ss received 200-Hz medial septal stimulation which does not induce theta. The 2 remaining control groups continued as in the training phase. In Phase 3, FR-10 responding was extinguished in all Ss. Induction of hippocampal theta depressed responding and led to greater resistance to extinction in the experimental group compared with the other groups. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Interaction of Pavlovian conditioning with a zero operant contingency: Chronic exposure to signaled inescapable shock maintains learned helplessness effects.

Four studies, with 372 male Holtzman rats, examined the effect of Pavlovian contingencies and a zero operant contingency (i.e., uncontrollability) on subsequent shock-escape acquisition in the shuttle box using triads consisting of escapable-shock (ES), yoked inescapable-shock (IS), and no-shock (NS) rats. After exposure to 50 signals and shocks per session for 9 sessions, interference with shuttlebox escape acquisition for IS Ss was a monotonically increasing function of the percentage of signal–shock pairings during training (Exp I), with 50% pairings producing little or no impairment. Without regard to signaling, ES Ss performed as well as NS Ss. Exp II demonstrated that training and test conditions led to substantial and equal impairment in IS Ss preexposed for 1 session to 100 or 50% signal–shock pairings or to unsignaled shocks. In Exp III, chronic exposure to 100% signaled ISs resulted in impairment only if the signal (light) was present during the shuttlebox test. The continuous presence of the signal during the test contrasted with its discrete (5-sec) presentation during training and suggested that an antagonistic physiological reaction rather than a specific competing motor response had been conditioned. Exp IV provided evidence for possible conditioned opioid mediation. Findings suggest that chronic exposure to uncontrollable shocks maintains the impairment produced by acute exposure only if the shocks are adequately signaled. (63 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Parallel augmentation of hippocampal long-term potentiation, theta rhythm, and contextual fear conditioning in water-deprived rats.

The influence of water deprivation on hippocampal long-term potentiation (LTP), theta rhythm, and contextual fear conditioning in 56 adult male rats was examined. In Exp 1, hippocampal EEG activity and perforant path LTP were assessed in pentobarbital-anesthetized rats. Water deprivation did not affect baseline cell excitability or low-frequency synaptic transmission in the dentate gyrus, but it increased the magnitude of perforant path LTP and elevated the proportion of theta rhythm in the EEG. In Exp 2, rats were classically conditioned to fear a novel context through the use of aversive footshocks. Water deprivation facilitated the rate of contextual fear conditioning but did not alter the asymptote of learning. Exp 3 demonstrated that the facilitation of contextual fear conditioning was not due to a change in unconditional shock sensitivity. These results suggest that water deprivation exerts an influence on contextual fear conditioning by modulating hippocampal LTP and theta rhythm and that these processes serve to encode contextual information during learning. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Effects of selective forebrain noradrenaline loss on behavioral inhibition in the rat.

In Exp I, virtually total depletion of cortical and hippocampal noradrenaline (NA) by stereotaxic injection of 6-hydroxydopamine into the fibers of the dorsal noradrenergic bundle produced no impairment in acquisition learning of a runway response for food reward by 10 male Wistar rats. Extinction of this response, once learned, was markedly slower in the treated group than in 10 controls, the treated animals perseverating in rapid running to the goal box even with no food present there. Similarly, in Exp II with the same Ss, no impairment was found on acquisition of a continuously reinforced leverpressing response for food. Extinction of this response, however, was again slower in the treated group. Exp III showed that the subsequent acquisition of a successive light-dark discrimination task was also slower in the same treated Ss, with these animals perseverating in responding to the negative stimulus. Although selective forebrain NA loss does not impair the acquisition of appetitive responses, the suppression of responses in the absence of reward is impeded. A parallel is drawn with those effects found classically after surgical lesion to the hippocampus. (35 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Classical conditioning of hippocampal theta patterns in the rat.

Electrical stimulation (88 Hz) of the lateral hypothalamus elicited a sustained theta response at hippocampal recording sites in 11 male Sprague-Dawley rats immobilized with succinylcholine. By pairing this UCS with a 10-sec presentation of a light, conditioned theta responses were demonstrated in as few as 40 trials. Spectral analysis of hippocampal bioelectric patterns during acquisition, extinction, and reconditioning indicated that the earliest change as a result of conditioning was a loss of power in EEG frequency below 8 Hz, followed by the development of a peak at 8 Hz with further conditioning. Extinction was associated with an increase in power of the frequencies below 8 Hz. When the conditioned Ss were tested in the absence of the neuromuscular blocking agent, the CS elicited a theta response that was associated with slow motor activity on 70% of the trials. (16 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Instrumental conditioning of hippocampal theta and subsequent response persistence.

Following bar-press shaping of 24 male albino rats to a discrete-trial fixed-ratio (FR) 5 schedule of reinforcement; 16 were trained to produce either hippocampal theta (7.5-8.5 Hz) or nontheta (4-5 Hz) activity, while 8 served as a matched interreinforcement time control (yoked to the theta group). All Ss then returned to the FR-5 schedule on the lever response for 15 days, followed by 12 days of extinction. Results indicate that hippocampal theta production was learned more slowly than nontheta production but resulted in increased resistance to extinction of the subsequently performed lever response. These data are viewed as supporting the hippocampal theta-frustration hypothesis proposed by H. Glazer in 1972. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Effects of naltrexone and signaling inescapable electric shock on nociception and gastric lesions in rats.

Two experiments, with 144 male Long-Evans hooded rats, examined the antinociceptive effects of signaled shock and its physiological underpinnings. In Exp I, Ss were exposed to 1 of 3 shock conditions: no shock, unsignaled shock, and signaled (by a 10-sec, 1,000-Hz tone) shock. In each condition, Ss were tested hourly in the absence of tones for nociception, with vocalization to shock used as the behavioral measure. Ss receiving signaled shocks had stomach ulcer scores intermediate between those of no-shock and unsignaled shock Ss. Signaled-shock Ss also displayed a pronounced vocalization antinociception effect. This suggested that signaled shock may be less aversive. Exp II investigated a possible role of endogenous opiate peptides in these effects. Ss received hourly injections of either the opiate antagonist naltrexone (7 mg/kg, ip) or saline. There were no significant effects of naltrexone on either stomach pathology or nociception scores. The same effects of signaled shock were obtained as in Exp I. It is concluded that the role of endogenous opiates in the effects of signaled shock seen here is minimal. (38 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

The septohippocampal cholinergic system and classical conditioning of the rabbit's nictitating membrane response.

28 New Zealand albino rabbits received bilateral microinjections of scopolamine (1 μl) or saline into either the dorsal hippocampus (Exp I) or the medial septal nucleus (Exp II). Ss then underwent classical conditioning of the nictitating membrane response in which a light served as a CS and eye shock served as the UCS. Results indicate that whereas hippocampal injections of scopolamine had no effect on conditioning, scopolamine injected into the medial septum retarded acquisition of the response. A 3rd experiment indicated that this retardation of conditioning was not due to changes in sensitivity to either the CS or UCS. Results are discussed in terms of accumulating evidence that manipulations that produce certain patterns of activity in the hippocampus are detrimental to acquisition of the nictitating CR. (43 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Failure to learn to escape in rats previously exposed to inescapable shock depends on nature of escape response.

Results of previous studies show that dogs exposed to inescapable shocks in a Pavlov harness subsequently fail to learn to escape shock in a shuttle box. The present 6 experiments attempted to replicate this finding with male Sprague-Dawley rats (N = 182). In agreement with many previous investigations, Exp I found that Ss exposed to inescapable shock did not fail to learn to escape in a shuttle box. Exp II, III, and IV varied the number, intensity, and temporal interval between inescapable shocks and did not find failure to learn in the shuttle box. An analysis of responding in the shuttle box revealed that Ss shuttled rapidly from the very 1st trial, whereas dogs acquire shuttling more gradually. Exp V and VI revealed that Ss exposed to inescapable shock failed to learn to escape when the escape response was one that was acquired more gradually. Exp V utilized a double crossing of the shuttle box as the escape response and Exp VI utilized a wheel-turn response. (20 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Hippocampal interneuron activity in unanesthetized rats: relationship to the sleep-wake cycle

Evoked population spikes and interneuronal discharges were recorded throughout the sleep-wake cycle in hippocampal regions CA1 and dentate gyrus (DG) of ten chronically implanted rats. During quiet wakefulness (QW) and slow-wave-sleep (SWS) (non-theta rhythm states), the primary shock of paired stimuli evoked in CA1 both high amplitude population spikes and multiple interneuron discharges when compared to active wakefulness (AW) and rapid-eye-movement (REM) sleep (theta rhythm states). A second shock was delivered to CA1 afferents 60 ms after the first shock. This second shock evoked a small population spikes during non-theta states, whereas it evoked higher amplitude population spikes in theta states. The second shock also evoked unit interneuron discharges in non-theta states but not in theta states. In the dentate gyrus, identical primary afferent stimulation evoked similar interneuron activity and uniform amplitude population spikes throughout the sleep-wake cycle. In contrast, the secondary shocks evoked a striking potentiation of the field population spike during sleep, SWS and REM sleep compared to AW and OW. Evoked DG interneuron spikes following the second population spike were greater in number during SWS compared to the other stages. Our findings suggest that hippocampal field potentials and interneuron activity recorded in vivo are regionally regulated, have unique state-dependent expression and are strongly influenced by inhibitory feed-forward mechanisms.     

An investigation of responding on schedules of electrical brain-stimulation reinforcement.

In Exp I, the leverpressing responses of rats (8 male hooded Long-Evans) were maintained by reinforcement consisting of single trains of electrical stimulation of the brain (ESB) presented on random-interval (RI) schedules ranging in value from RI 3-min to RI 10-min. Both the cumulative response patterns and the relationship of response rates to reinforcement density were similar to those observed for Ss reinforced conventionally. In Exp II with 19 male hooded Long-Evans rats, leverpressing was reinforced with single trains of signalled ESB, unsignalled response-contiguous ESB, or sweetened condensed milk presented on random-ratio schedules. Most of the ESB-reinforced Ss and half of the milk-reinforced Ss stopped responding at ratio values exceeding 50:1, while the remaining Ss responded at higher ratios. Response rates were higher at lower ratio values for the ESB groups than for the milk group, but as ratio values increased, all groups showed similar decreases in rate. All Ss were observed to initiate responding faster than inexperienced controls, and priming was not required. (French summary) (24 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Pavlovian and instrumental determinants of response suppression in the pigeon.

Exp I demonstrated the formation of a discriminated punishment effect in the absence of a conditioned emotional response. Electric shocks were delivered at random intervals to 3 naive male White Carneaux pigeons pecking for food on a variable-interval schedule. During a 1-min visual conditioned stimulus (CS), scheduled shocks were delayed until a response occurred (punishment). Differential suppression to the CS was observed in addition to overall suppression. Suppression was related to shock intensity. In Exp II with the same Ss, CS suppression was related to the CS and was not an artifact of response pattern or discrimination of shock patterns. The punishment contingency without the CS did not suppress behavior, and the CS without the punishment contingency did not relieve suppression. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Social behaviour in rats during escape from shock.

Following individual training in a leverpress shock escape situation, 6 pairs of male albino Wistar rats were exposed to the shock escape schedule. Escape performance of pretrained Ss deteriorated during the social session regardless of whether the pair involved a naive or 2nd pretrained S. Reliable shock-elicited fighting was observed only in pairs in which both Ss had been pretrained to escape shock, whereas no fighting occurred in paired naive Ss during exposure to the escape schedule. Disruption of individual escape performance, as well as bizarre social results (e.g., Ss making leverpressing or "holding" responses on each other), are discussed in terms of R. C. Bolles's theory of species specific defense reactions. (French summary) (19 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Slower acquisition of positively reinforced behavior following medial, but not dorsolateral, septal lesions in rats.

Examined the effects of medial septal lesions (MSLs) and dorsolateral septal lesions (DSLs) on acquisition of leverpressing for water established with a modified autoshaping task. 22 male Sprague-Dawley rats were randomly assigned to receive either MSLs, DSLs, or control operations. Results show that, following MSLs, leverpressing responses had long latencies and were fewer than following either DSLs or control operations. However, during later sessions, MSL Ss started to press. It is suggested that the long response latencies and low response rates shown initially by MSL Ss may have been due to delayed classical conditioning. Following MSLs, the acetylcholinesterase activities of the different subdivisions of the hippocampus correlated positively with the number of leverpress responses emitted during Session 1. DSL Ss acquired the task just as fast as the control group and showed higher response rates than the other 2 groups during the final sessions. These findings are ascribed to generally increased locomotor activity. (24 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Suppressive and enhancing effects of footshock on food-reinforced operant responding following septal lesions in rats.

Measured effect of septal lesions on suppression of an intermittently food-reinforced lever press by contingent and noncontingent footshock, using a total of 48 experimentally naive male hooded Lister rats in 2 experiments. Ss with septal damage maintained higher response rates than did intact Ss under both contingent and noncontingent shock. Furthermore, the difference in suppression produced by the contingent and noncontingent conditions was approximately the same for the experimental Ss and controls. In Exp II performance was measured during counter-conditioning in which the correlation between contingent shock and positive reinforcement was varied. Ss with septal lesions responded at higher rates than did controls. When only reinforced responses were punished, this lesion-induced elevation represented an increase above baseline performance without punishment. This finding suggests that the effect of septal damage on appetitive instrumental performance cannot be due solely to a deficit in response inhibition. (27 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Rats prefer signaled over unsignaled shock-free periods.

Three experiments with 48 female rats investigated Ss' preference for a schedule of shock containing signaled shock-free periods (SSF schedule) or the same shock schedule without signals (unsignaled schedule). Exp I was a replication of the shuttle-box experiment reported by M. S. Fanselow (see record 1981-00807-001), in which rats preferred unsignaled shock over the SSF schedule. Contrary to Fanselow's results, Ss in Exp I failed to develop a preference. In Exp II, forced-exposure training was added, testing was extended from 2 165-min sessions to 8, and a reversal phase was added. Results show that 3 of 4 Ss preferred the SSF schedule, and 2 of these continued to prefer it after reversal. In Exp III, forced-exposure training was eliminated, but signals were lengthened from 30 to 60 sec, and the test was extended to 12 sessions. Results show that Ss choosing between the unsignaled and SSF schedules chose the SSF schedule, whereas a random control group chose the unsignaled schedule. Findings indicate that choice between active schedules is determined by the relative aversiveness of all stimuli presented and not, as implied by contextual fear analysis, by that of contextual stimuli alone. (25 ref) (PsycINFO Database Record (c) 2011 APA, all rights reserved)     

Mechanisms underlying the plasticity of hippocampal stimulation-induced reward.

It has been reported that the slow acquisition of hippocampal self-stimulation can be markedly facilitated by pretreatment with a program of repeated daily hippocampal stimulation (kindling). Three experiments with 68 male Long-Evans rats investigated the neurophysiological basis of this effect. Exp I demonstrated that unilateral stimulation pretreatment produced a facilitation of learning to leverpress for stimulation delivered to the contralateral hippocampal electrode. Thus, there was a transfer of facilitation. In Exp II, it was shown that this transfer effect was not affected by lesion of the originally kindled focus, a result suggesting that the facilitated acquisition was not due to feedback to the kindled site. In Exp III, electrical activity during hippocampal self-stimulation was examined to explore the possible correlation between hippocampal reward and epileptiform activity. No relation was apparent: Leverpressing persisted even when no afterdischarge was elicited. Findings are consistent with the hypothesis that the facilitatory effect of stimulation reflects the development of transsynaptic potentiation. (28 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Comparison of behavioral deficits caused by lesions in septal and ventromedial hypothalamic areas of female rats.

41 female Holtzman rats with lesions in ventromedial hypothalamic (VMH) area and 37 Ss with lesions in septal area were compared with 30 normal Ss for passive-avoidance performance (Exp I), reversal learning (Exp II), and spontaneous alternation (Exp III). Lesions in both septal and VMH areas produced a deficit in passive-avoidance performance, a greater number of errors in reversal learning, and reduced spontaneous alternation in a -maze. The qualitatively similar behavioral deficits produced by septal and VMH lesions suggests that at least part of the functions of both of these areas may overlap in a single system. An attempt was made to identify such a functional system, and an explanation for the behavioral deficits produced by VMH damage was offered. (20 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Exposure to uncontrollable stress alters withdrawal from morphine.

Examined the influence of the controllability/uncontrollability of shock as a stressor on the severity of subsequent morphine withdrawal in 2 experiments with 84 male Holtzman rats. In Exp I (36 Ss), Ss that received 2 daily sessions of 80 yoked-inescapable shocks, in contrast to those given 80 escapable shocks or restrained without shock, showed an enhanced series of correlated withdrawal behaviors (i.e., mouthing, teeth chattering, head/body shakes) 24 hrs later when injected with morphine sulfate (5 mg/kg) followed by a naloxone HCl (5 mg/kg) challenge. In Exp II (48 Ss), this finding was replicated with escape-yoked-restrained Ss given saline injections during the pretreatment phase, but the impact that inescapable shock had on later precipitated withdrawal was completely blocked when Ss were administered naltrexone HCl (14 mg/kg) before each shock session. Findings are discussed in terms of the capability of inescapable shock to activate an endogenous opiate system, thereby leading to a sensitization of release or receptor processes that could protentiate later morphine withdrawal. (56 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

DSP4 (N-2-chloroethyl-N-ethyl-2-bromobenzylamine), a new noradrenaline neurotoxin, and stimulus conditions affecting acquisition of two-way active avoidance.

Several CS and UCS variables known to affect the rate of acquisition of the 2-way active avoidance task were investigated in rats treated with the novel selective noradrenaline neurotoxin DSP4 (50 mg/kg, ip). 234 male Sprague-Dawley rats were used in 6 experiments. Although the DSP4 Ss did not demonstrate the linear relation between CS duration and avoidance acquisition to the same extent as controls, their avoidance performance was as drastically disrupted as that of the controls both by preexposure to the CS and by increasing levels of shock intensity. DSP4 Ss also evidenced fear retention for the shuttle box cues previously associated with inescapable shocks to as marked a degree as control Ss. Biochemical data indicated profound noradrenaline depletion in the cortex and hippocampus and a lesser depletion in the hypothalamus. Findings offer a behavioral characterization of the consistent DSP4-induced impairment of 2-way active avoidance acquisition. (46 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)