Effects of prepartum dietary cation-anion difference and source of vitamin D in dairy cows: Lactation performance and energy metabolism

The objectives of this experiment were to evaluate the effects of feeding diets with 2 dietary cation-anion difference (DCAD) levels and supplemented with either cholecalciferol (CH) or calcidiol (CA) during late gestation on lactation performance and energetic metabolism in dairy cows. The hypothesis was that combining a prepartum acidogenic diet with calcidiol supplementation would benefit peripartum Ca metabolism and, thus, improve energy metabolism and lactation performance compared with cows fed an alkalogenic diet or cholecalciferol. Holstein cows at 252 d of gestation were blocked by parity (28 nulliparous and 51 parous cows) and milk yield within parous cows, and randomly assigned to 1 of 4 treatments arranged as a 2 × 2 factorial, with 2 levels of DCAD (positive, +130, and negative, ?130 mEq/kg) and 2 sources of vitamin D, CH or CA, fed at 3 mg per 11 kg of diet dry matter (DM). The resulting treatment combinations were positive DCAD with CH (PCH), positive DCAD with CA (PCA), negative DCAD with CH (NCH), or negative DCAD with CA (NCA), which were fed for the last 21 d of gestation. After calving, cows were fed the same lactation diet. Body weight and body condition were evaluated prepartum and for the first 49 d postpartum. Blood was sampled thrice weekly prepartum, and on d 0, 1, 2, 3, and every 3 d thereafter until 30 d postpartum for quantification of hormones and metabolites. Lactation performance was evaluated for the first 49 d postpartum. Feeding a diet with negative DCAD reduced DM intake in parous cows by 2.1 kg/d, but no effect was observed in nulliparous cows. The negative DCAD reduced concentrations of glucose (positive = 4.05 vs. negative = 3.95 mM), insulin (positive = 0.57 vs. negative = 0.45 ng/mL), and insulin-like growth factor-1 (positive = 110 vs. negative = 95 ng/mL) prepartum. Treatments did not affect DM intake postpartum, but CA-supplemented cows tended to produce more colostrum (PCH = 5.86, PCA = 7.68 NCH = 6.21, NCA = 7.96 ± 1.06 kg) and produced more fat-corrected milk (PCH = 37.0, PCA = 40.1 NCH = 37.5, NCA = 41.9 ± 1.8 kg) and milk components compared with CH-supplemented cows. Feeding the negative DCAD numerically increased yield of fat-corrected milk by 1.0 kg/d in both nulliparous and 1.4 kg/d in parous cows. Minor differences were observed in postpartum concentrations of hormones and metabolites linked to energy metabolism among treatments. Results from this experiment indicate that replacing CH with CA supplemented at 3 mg/d during the prepartum period improved postpartum lactation performance in dairy cows.     

Effect of source and amount of vitamin D on function and mRNA expression in immune cells in dairy cows

Objectives were to determine the effect of supplementing 2 sources of vitamin D, cholecalciferol (CH) or calcidiol (CA), at 1 (1mg) or 3 mg/d (3mg) prepartum on concentrations of vitamin D metabolites in plasma, measures of innate immune function, and leukocyte mRNA expression. Parous Holstein cows (n = 99) were assigned to a daily treatment administered as top-dress containing either 1 or 3 mg of CH (CH1 or CH3) or of CA (CA1 or CA3) from 250 d of gestation until calving. Plasma concentrations of vitamin D, immune cell population in blood, cell adhesion markers, and granulocyte phagocytosis and oxidative burst were evaluated pre- and postpartum. The mRNA expression in leukocytes was determined at 270 d of gestation and 3 d postpartum for genes involved in cell migration, pathogen recognition receptors, cell signaling, cytokines, antimicrobial mechanisms, oxidative burst, and Ca and vitamin D metabolism. Concentrations of vitamin D₃ increased in cows fed CH, whereas those of 25-hydroxyvitamin D₃ increased in cows fed CA. Percentage of granulocytes from total leukocytes differed with amount of vitamin D pre- (1mg = 24.5 vs. 3mg = 37.9%) and postpartum (1mg = 22.0 vs. 3mg = 31.0%), thus shifting mononuclear cells in the opposite direction pre- (1mg = 75.5 vs. 3mg = 62.1%) and postpartum (1mg = 78.0 vs. 3mg = 69.0%). Granulocytes displaying phagocytosis (1mg = 69.0 vs. 3mg = 62.9%) and intensity of phagocytosis prepartum (1mg = 7.46 vs. 3mg = 7.28) tended to be less in cows fed 3mg compared with 1mg. During prepartum, CA increased mRNA expression of genes related to cell adhesion and migration (CD44, ICAM1, ITGAL, ITGB1, LGALS8, SELL), pathogen recognition receptor (NOD2, TLR2, TLR6), cell signaling (FOS, JUN, NFKB2), cytokine signaling (IL1B, IL1R1, IL1RN), antimicrobial mechanisms (CTSB, LYZ), and Ca metabolism (ATP2B1, STIM1, TRPV5) compared with CH. Similarly, postpartum, CA increased mRNA expression of genes related to cell adhesion and migration (CXCR2, SELL, TLN1), cell signaling (AKT2), cytokines (CCL2, IL1R1, ILRN), antimicrobial mechanisms (DEFB3), oxidative burst (RAC2), and calcium metabolism (CALM3) compared with CH. Feeding additional vitamin D in the last 3 wk of gestation changed the profile of blood leukocytes and attenuated granulocyte phagocytosis during the transition period, whereas supplementing CA prepartum increased mRNA expression of genes involved in immune cell function, including genes related to pathogen recognition and antimicrobial effects of leukocytes.     

Effect of source and amount of vitamin D on serum concentrations and retention of calcium,magnesium, and phosphorus in dairy cows

The objectives of the experiment were to determine the effects of supplementing 2 amounts of 25-hydroxyvitamin D₃ (calcidiol; CAL) compared with equal amounts of vitamin D₃ (cholecalciferol; CHOL) on serum concentrations, absorptions, and retentions of Ca, Mg, and P in periparturient dairy cows. One hundred seventy-seven (133 parous and 44 nulliparous) pregnant Holstein cows were enrolled in the experiment. Cows were blocked by parity and previous lactation milk yield (parous) or genetic merit for energy-corrected milk yield (nulliparous) and assigned randomly to receive 1 or 3 mg/d of CAL or CHOL in a 2 × 2 factorial arrangement of treatments. Treatments were provided to individual cows as a top-dress to the prepartum diet from 250 d gestation until parturition. The prepartum diet had a dietary cation-anion difference of ?128 mEq/kg of dry matter. All cows were fed a common postpartum diet containing 46 μg of vitamin D₃/kg of dry matter without further supplementation of treatments. Concentrations of vitamin D metabolites, Ca, Mg, and P in serum were measured pre- and postpartum, in addition to total-tract digestibility and urinary excretion of Ca, Mg, and P in the prepartum period. Feeding 3 mg compared with 1 mg of CAL increased serum 25-hydroxyvitamin D₃ (CAL1 = 94 vs. CAL3 = 173 ± 3 ng/mL). In comparison, the increment in serum 25-hydroxyvitamin D₃ from feeding 3 mg compared with 1 mg of CHOL was small (CHOL1 = 58 vs. CHOL3 = 64 ± 3 ng/mL). Feeding CAL increased prepartum concentration of P in serum compared with CHOL (CHOL = 1.87 vs. CAL = 2.01 ± 0.02 mM), regardless of the amount fed, but neither source nor amount affected prepartum Ca or Mg in serum. Feeding CAL increased serum Ca and P for the first 11 d postpartum compared with CHOL (CHOL = 2.12 vs. CAL = 2.16 ± 0.01 mM serum Ca; CHOL = 1.70 vs. CAL = 1.78 ± 0.02 mM serum P) but the amount of vitamin D did not affect postpartum concentrations of Ca, Mg, and P in serum. Feeding CAL increased prepartum apparent digestibility of Ca compared with CHOL (CHOL = 26.6 vs. CAL = 33.5 ± 2.8%) but treatments did not affect Ca retention prepartum. Neither source nor amount of vitamin D affected Mg and P apparent digestibility, but CAL decreased the concentration of P excreted in urine during the prepartum period (CHOL = 1.8 vs. CAL = 0.8 ± 0.3 g/d). Calcidiol tended to increase the amount of Ca secreted in colostrum (CHOL = 9.1 vs. CAL = 11.2 ± 0.9 g/d) and Ca excreted in urine postpartum (CHOL = 0.4 vs. CAL = 0.6 ± 0.1 g/d) compared with CHOL. Collectively, feeding CAL at 1 or 3 mg/d compared with CHOL in the last 24 d of gestation is an effective way to increase periparturient serum P concentration and postpartum serum Ca of dairy cows fed a prepartum diet with negative DCAD.     

Effects of prepartum dietary cation-anion difference and source of vitamin D in dairy cows: Health and reproductive responses

The objectives of the experiment were to evaluate the effects of feeding diets with distinct dietary cation-anion difference (DCAD) levels and supplemented with 2 sources of vitamin D during the prepartum transition period on postpartum health and reproduction in dairy cows. The hypotheses were that feeding acidogenic diets prepartum would reduce the risk of hypocalcemia and other diseases, and the benefits of a negative DCAD treatment on health would be potentiated by supplementing calcidiol compared with cholecalciferol. Cows at 252 d of gestation were blocked by parity (28 nulliparous and 52 parous cows) and milk yield within parous cows, and randomly assigned to 1 of 4 treatments arranged as a 2 × 2 factorial, with 2 levels of DCAD, positive (+130 mEq/kg) or negative (?130 mEq/kg), and 2 sources of vitamin D, cholecalciferol or calcidiol, fed at 3 mg for each 11 kg of diet dry matter. The resulting treatment combinations were positive DCAD with cholecalciferol (PCH), positive DCAD with calcidiol (PCA), negative DCAD with cholecalciferol (NCH), and negative DCAD with calcidiol (NCA), which were fed from 252 d of gestation to calving. After calving, cows were fed the same lactation diet supplemented with cholecalciferol at 0.70 mg for every 20 kg of dry matter. Blood was sampled 7 d before parturition, and at 2 and 7 d postpartum to evaluate cell counts and measures of neutrophil function. Postpartum clinical and subclinical diseases and reproductive responses were evaluated. Feeding a diet with negative DCAD eliminated clinical hypocalcemia (23.1 vs. 0%) and drastically reduced the incidence and daily risk of subclinical hypocalcemia, and these effects were observed in the first 48 to 72 h after calving. The diet with negative DCAD tended to improve the intensity of oxidative burst activity of neutrophils in all cows prepartum and increased the intensity of phagocytosis in parous cows prepartum and the proportion of neutrophils with killing activity in parous cows postpartum (58.5 vs. 67.6%). Feeding calcidiol improved the proportion of neutrophils with oxidative burst activity (60.0 vs. 68.7%), reduced the incidences of retained placenta (30.8 vs. 2.5%) and metritis (46.2 vs. 23.1%), and reduced the proportion of cows with multiple diseases in early lactation. Combining the negative DCAD diet with calcidiol reduced morbidity by at least 60% compared with any of the other treatments. Cows with morbidity had lower blood ionized Ca and serum total Ca concentrations than healthy cows. Treatments did not affect the daily risk of hyperketonemia in the first 30 d of lactation. Despite the changes in cow health, manipulating the prepartum DCAD did not influence reproduction, but feeding calcidiol tended to increase the rate of pregnancy by 55%, which reduced the median days open by 19. In conclusion, feeding prepartum cows with a diet containing a negative DCAD combined with 3 mg of calcidiol benefited health in early lactation.     

Effect of N-acetyl-l-methionine supplementation on lactation performance and plasma variables in mid-lactating dairy cows

The objective of current study was to investigate the effect of N-acetyl-l-methionine (NALM) supplementation on lactation performance and plasma variables in mid-lactating dairy cows. Forty-eight multiparous cows were blocked into 12 groups based on parity, days in milk, and milk production and were randomly assigned to 1 of the 4 treatments: 0, 15, 30, or 60 g/d of NALM per cow to supplement the basal diet. The experiment was conducted over a 13-wk period, with the first week as adaptation. The yields of milk, fat-corrected milk, and milk lactose were increased quadratically, and energy-corrected milk yield tended to increase with increased NALM supplementation in a quadratic manner. The dry matter intake, milk protein yield, milk fat yield, contents of milk composition (protein, fat, lactose, total solids, and milk urea nitrogen), feed efficiency, and body weight change were not affected by NALM supplementation. In addition, plasma methionine concentration was increased quadratically, and proline, total nonessential AA, and total AA concentrations were significantly higher in the 30 g/d group compared with that of the control group. However, other AA and total essential AA concentrations were not affected with supplementation of NALM. Adding NALM increased concentrations of total protein and globulin in plasma, but decreased plasma urea nitrogen concentration in a quadratic manner. Meanwhile, plasma malonaldehyde concentration decreased linearly as doses of NALM addition increased. Our results suggested that the supplementation of NALM improved milk yield and protein synthesis in the liver, and lowered lipid peroxidation in mid-lactating dairy cows.     

Effect of parity and stage of lactation on feed sorting behavior of lactating dairy cows

The objectives of this study were to determine if feed sorting differs between primiparous (PP) and multiparous (MP) cows, if sorting changes from the period of peak lactation to peak dry matter intake (DMI), and whether feed sorting affects efficiency of production. Data on DMI, milk production, feed sorting (particle size of offered and refused feed), and energy status (plasma nonesterified fatty acid, β-hydroxybutyrate, insulin and glucose concentration) were collected on 30 PP and 30 MP lactating Holstein dairy cows, individually housed and fed in tie-stalls, during 3 wk (wk 2, 6, and 10) over 10 wk of a lactation study. Cows averaged 53, 81, and 109 ± 10 d in milk (DIM) at the beginning of each of the 3 recording weeks. To determine sorting, feed samples were separated with a particle separator that had 3 screens (19, 8, and 1.18 mm) and a bottom pan, resulting in 4 fractions (long, medium, short, fine). Over the study period, MP cows consumed more DM and produced more milk than did the PP cows, but had similar efficiency of production (kg of milk/kg of DMI), and similar levels of plasma indicators of energy status. Across the study period, DMI increased, whereas milk yield decreased, resulting in decreased efficiency of milk production as cows moved further into lactation. All cows had higher nonesterified fatty acid and lower insulin concentrations in plasma at 53 DIM compared with at 81 and 109 DIM, suggesting they were mobilizing more body fat at that earlier stage of lactation. Across periods, all cows sorted against the longest ration particles, did not sort the medium ration particles, and sorted for fine ration particles; as a result, all cows consumed less neutral detergent fiber (NDF) and physically effective NDF (proportion of NDF retained on the 19- and 8-mm screens of the particle separator) than predicted. Greater selection against the longest ration particles was associated with greater efficiency of milk production; however, this sorting pattern also tended to be associated with lower milk fat percentage. The extent of the observed sorting against the longest, physically effective ration particles and for the finest ration particles was greater for PP cows than for MP cows across all 3 observation periods. Feed sorting remained consistent in cows across the DIM evaluated in this study and that this sorting behavior can affect the efficiency of milk production as well as milk fat percentage. Further, PP cows engage in more sorting of their ration than MP cows.     

Effects of dietary supplements of folic acid and vitamin B12 on metabolism of dairy cows in early lactation

The present experiment was undertaken to determine the effects of dietary supplements of folic acid and vitamin B₁₂ given from 3 wk before to 8 wk after calving on lactational performance and metabolism of 24 multiparous Holstein cows assigned to 6 blocks of 4 cows each according to their previous milk production. Supplementary folic acid at 0 or 2.6 g/d and vitamin B₁₂ at 0 or 0.5 g/d were used in a 2 × 2 factorial arrangement. Supplementary folic acid increased milk production from 38.0 ± 0.9 to 41.4 ± 1.0 kg/d and milk crude protein yield from 1.17 ± 0.02 to 1.25 ± 0.03 kg/d. It also increased plasma Gly, Ser, Thr, and total sulfur AA, decreased Asp, and tended to increase plasma Met. Supplementary B₁₂ decreased milk urea N, plasma Ile, and Leu and tended to decrease Val but increased homocysteine, Cys, and total sulfur AA. Liver concentration of phospholipids was higher in cows fed supplementary B₁₂. Plasma and liver concentrations of folates and B₁₂ were increased by their respective supplements, but the increase in plasma folates and plasma and liver B₁₂ was smaller for cows fed the 2 vitamins together. In cows fed folic acid supplements, supplementary B₁₂ increased plasma glucose and alanine, tended to decrease plasma biotin, and decreased K_m of the methylmalonyl-coenzyme A mutase in hepatic tissues following addition of deoxyadenosylcobalamin, whereas it had no effect when cows were not fed folic acid supplements. There was no treatment effect on plasma nonesterified fatty acids as well as specific activity and gene expression of Met synthase and methylmalonyl-coenzyme A mutase in the liver. Ingestion of folic acid supplements by cows fed no supplementary B₁₂ increased total lipid and triacylglycerols in liver, whereas these supplements had no effect in cows supplemented with B₁₂. The increases in milk and milk protein yields due to folic acid supplements did not seem to be dependent on the vitamin B₁₂ supply. However, when vitamin B₁₂ was given in combination with folic acid, utilization of the 2 vitamins seems to be increased, probably more so in extrahepatic tissues. Metabolic efficiency seems also to be improved as suggested by similar lactational performance and dry matter intake for cows fed supplementary folic acid but increased plasma glucose and decreased hepatic lipids in cows fed folic acid and vitamin B₁₂ together.     

Effect of prepartum administration of recombinant bovine somatotropin on health and performance of lactating dairy cows

A double-blind, randomized clinical trial was conducted in 5 commercial dairy herds in southern Ontario with 1,362 cows enrolled to evaluate the effect of prepartum administration of recombinant bovine somatotropin (rbST) on health and performance. Cows were randomly assigned to receive either 325 mg of sometribove zinc suspension (n = 680) or a placebo injection (n = 682; control) subcutaneously every 14 d until calving. Treatments started 28 to 22 d before expected calving, with a maximum of 3 treatments per cow. Serum samples taken at the time of enrollment, 1 wk before calving, and weekly for 3 wk after calving were analyzed for nonesterified fatty acids (NEFA), β-hydroxybutyrate (BHBA), glucose, aspartate aminotransferase, calcium, and haptoglobin. Diseases were recorded by farm staff. Incidences of clinical ketosis, clinical mastitis, displaced abomasum, metritis, retained placenta, milk fever, and lameness were similar between groups. Body condition score was lower for treated than for control cows at 3 wk after calving (3.13 and 3.17, respectively). Serum NEFA tended to be higher for treated than for control cows by 0.01 mmol/L. Overall BHBA was not different between groups, but BHBA for treated cows was higher in wk 1 after calving (750 and 698 μmol/L, respectively) and tended to be higher in wk 2 after calving (779 and 735 μmol/L, respectively). Incidence of hyperketonemia was similar between groups. Treated cows had higher serum glucose compared with control cows (2.8 and 2.7 mmol/L, respectively). We detected no differences in serum aspartate aminotransferase, calcium, or haptoglobin between groups. Milk yield was recorded daily for each cow for 63 d, and did not differ between groups (37.1 ± 0.5 kg and 36.7 ± 0.5 kg, respectively) but we detected a tendency for treated cows to produce 0.8 kg/d more milk than control cows in wk 1 after calving. We observed no difference between groups in the time from calving to first insemination or the probability of pregnancy at the first insemination. Groups did not differ in the proportion of anovular cows at 53 ± 3 d in milk based on serum progesterone measured from a subset of cows (38.0 and 34.3%, respectively, for treated and control groups). We found no difference between groups in dry matter intake from 21 d before calving to 63 d after calving in a subset of cows (17.4 ± 0.4 and 17.5 ± 0.4 kg/d, respectively). Based on results of the current study, biweekly (every 14 d) administration of rbST before calving to prevent disease and enhance performance is not recommended.     

Extended lactation in high-yielding dairy cows. I. Effects on reproductive measurements

The objective of this prospective field study was to evaluate the effects of extending the lactation period on various reproductive measurements of high-yielding Holstein cows. On 40 d in milk (DIM), cows were gynecologically examined (transrectal palpation, sonography, vaginoscopy). Cows without signs of clinical endometritis were blocked by parity and were randomly allocated to 1 of 3 experimental groups with a voluntary waiting period (VWP) of 40, 120, and 180 d, respectively (G40, n = 135; G120, n = 141; G180, n = 139). Cows of G120 and G180 were reexamined at the end of the VWP. If natural estrus was detected within 46 d after the end of the VWP, an artificial insemination was performed. If no estrus was detected, the respective cows were synchronized by applying the classical Ovsynch protocol. We found no difference in the proportion of cows in which estrus was detected between 40 to 86 DIM or in the days to first estrus between the 3 groups. Estrus detection in this period was lower in cows with body condition score <3 on 90 DIM compared with body condition score ≥3 (61.5 vs. 76.0%) and in cows with high energy-corrected milk production (ECM) on 92 DIM [58.6 vs. 70.1%, for cows with higher and lower than the median (39.9 kg) ECM, respectively]. The proportion of cows that estrus was detected within 46 d after the VWP was greater in G120 (88.9%) and G180 (90.8%) compared with G40 (70.4%). These effects were more apparent in cows with high ECM. The rate of estrus detection and of becoming pregnant in this period was greater for G120 (hazard ratio = 2.2 and 1.6, respectively) and for G180 (hazard ratio = 2.4 and 1.8) compared with G40. Cows in both groups with extended lactation had greater overall first service conception rates (G120 = 48.9%; G180 = 49.6%) and a lower number of services per pregnant cow (G120 = 1.56 ± 0.1; G180 = 1.51 ± 0.1) compared with G40 (36.6%; 1.77 ± 0.1). We observed no difference in pregnancy loss or in the proportion of cows culled up to 305 d of lactation between the 3 groups. The number of Ovsynch protocols per 1,000,000 kg of ECM was reduced by 75% in G180 and by 74% in G120 compared with G40 (5.9 vs. 7.1 vs. 25.1). In conclusion, extending the lactation of dairy cows can improve main reproductive measurements in high-yielding cows.     

Associations of neonatal acute phase response with first lactation performance in dairy cows

During the first 3 wk of life, the immune system of newborn ruminants starts to work, as indicated by fluctuations in the concentrations of proinflammatory cytokines and acute phase proteins (APP). They have been shown to be markers for short and long-term weight gain in ruminants. This observational study investigated these proteins as possible indicators of first lactation performance of dairy cows. A total of 117 dairy calves from a single farm were enrolled in the study. Serum and fecal samples were taken once a week for the first 3 wk of life. Cryptosporidium spp. infection and its treatment were monitored and accounted for in statistical analysis. The concentrations of the APP serum amyloid A (SAA) and haptoglobin (Hp), and the proinflammatory cytokines interleukin-6 (IL-6) and tumor necrosis factor α (TNF-α) were analyzed in serum. After the first lactation, health and performance data of the cows were retrieved, and associations between APP and cytokine concentrations with first lactation outcomes were investigated through linear and logistic regression. This study found a negative association between the concentration of Hp in the second week of life and average daily weight gain at one year. The SAA concentration measured during the second and third weeks of life was positively associated with age at first calving. IL-6, SAA, and Hp measured in the second week of life were positively associated with calving to conception interval. The concentrations of IL-6 and TNF-α during the first week of life were associated with higher odds of reproductive issues during the first lactation period. These markers can be used to help understand underlying processes that influence animal health and production. In conclusion, inflammatory responses during the first week of life are related to reproductive success, whereas the second and third weeks of life seem to influence the future productive performance in dairy cows.     

Reproductive performance of dairy cows is influenced by prepartum feed restriction and dietary fatty acid source

The objective of this study was to determine the effects of feed restriction and source of dietary fatty acids during the close-up dry period on postcalving reproductive performance of dairy cattle. Thirty-four days before expected calving, pregnant Holstein cows (n = 72; parity 1 to 5) were randomly assigned to 1 of 6 treatments. Treatments were ad libitum (AL) or 24% feed restriction (FR) in combination with 1 of 3 oilseed supplements at 8% of diet dry matter: canola, linola, or flax to enrich the rations with oleic, linoleic, or linolenic fatty acids, respectively. After calving, cows were fed a common lactation diet that contained no oilseeds. Measurements of uterus, corpus luteum, and follicles were obtained by ultrasonography twice weekly from 7 ± 1 d after calving until the first ovulation. Cows (n = 66) were subjected to timed artificial insemination (TAI), and pregnancy was determined 32 d later. Feed-restricted cows had lower dry matter intake and lost more body weight prepartum. Energy balance (Mcal/d) was negative in FR cows prepartum but they had a less severe negative energy balance postpartum. The dietary source of fatty acid did not affect energy balance. Cows fed AL had a higher incidence of uterine infections (10/37 vs. 2/35) but tended to have fewer ovarian cysts (2/37 vs. 7/35) than FR cows. Mean (±SE) interval from calving to uterine involution did not differ among dietary treatments (26.8 ± 1.8 d). Interval from calving to first ovulation was longer in cows fed canola than in those fed either linola or flax (34.7 ± 3.1 vs. 23.7 ± 3.2 and 21.0 ± 3.1 d, respectively). A greater percentage of cows fed AL conceived to the first TAI (47.1 vs. 18.8) and tended to have fewer mean days open (157 ± 10.8 vs. 191 ± 10.1) than cows fed FR. In summary, FR cows had a lower incidence of uterine infections, but they were less fertile as reflected by a lower percent pregnancy to first TAI and increased days open. Cows fed diets enriched in linoleic or linolenic fatty acids had a lesser incidence of ovarian cysts and ovulated sooner with no effect on energy balance or fertility.     

Effect of essential oils on ruminal fermentation and lactation performance of dairy cows

Three experiments (Exp.) were conducted to study the effects of dietary addition of an essential oil product (EO) based on eugenol and cinnamaldehyde (0, control, or 525 mg/d of Xtract 6965; Pancosma SA, Geneva, Switzerland) on ruminal fermentation, total-tract digestibility, manure gas emissions, N losses, and dairy cow performance. In Exp. 1 and 3, the EO supplement was added to the vitamin-mineral premix. In Exp. 2, EO was top-dressed. Experiments 1 and 2 were crossover designs with 20 multiparous Holstein cows each (including 4 and 8 ruminally cannulated cows, respectively) and consisted of two 28-d periods. Intake of dry matter did not differ between treatments. Most ruminal fermentation parameters were unaffected by EO. Concentrations of ammonia (Exp. 1), isobutyrate (Exp. 1 and 2), and isovalerate (Exp. 1) were increased by EO compared with the control. Apparent total-tract digestibility of nutrients was similar between treatments, except total-tract digestibility of neutral-detergent fiber, which was increased or tended to be increased by EO in Exp. 1 and 2. Manure emissions of ammonia and methane were unaffected by EO. Blood plasma and milk urea-N concentrations and urinary N losses were increased by EO compared with the control in Exp. 1, but not in Exp. 2. Average milk yield, 3.5% fat-corrected milk yield, and milk fat, protein, and lactose concentrations were unaffected by treatment. Urinary excretion of purine derivatives, a marker for microbial protein production in the rumen, was greater in cows receiving the EO diet in Exp. 1, but not in Exp. 2. In Exp. 3, 120 Holstein cows were grouped in pens of 20 cows/pen in a 12-wk experiment to study production effects of EO. Dry matter intake, milk yield (a trend for a slight decrease with EO), milk components, milk urea N, and feed efficiency were similar between treatments. Results from these studies indicate that supplementing dairy cows with 525 mg/d of Xtract 6965 had moderate effects on ruminal fermentation, but consistently increased ruminal isobutyrate concentration and tended to increase total-tract digestibility of neutral-detergent fiber. Under the conditions of these experiments, Xtract 6965 fed at 525 mg/d did not affect milk production or composition.     

Measuring the feeding behavior of lactating dairy cows in early to peak lactation

The objectives of this study were to: 1) objectively define meal criteria (minimum interval between meals) of free-stall housed cows fed via a feed alley, 2) determine which measures of feeding behavior were most repeatable, and 3) describe changes in the feeding behavior from early to peak lactation. An electronic monitoring system was used to record individual cow presence (hits; 6-s resolution) at the feed alley for 21 lactating cows for three 8-d periods: period 1, 35 +/- 16 (mean +/- SD), period 2, 57 +/- 16, and period 3, 94 +/- 16 DIM. A mixture distribution model was used to calculate the meal criterion (27.74 min) by fitting the log10 frequency distribution of the intervals between hits. The within-cow repeatability was highest for feeding activity (hits d(-1)) and intensity (hits per meal min), moderate for total daily mealtime (min d(-1)) and meal duration (min meal(-1)), and lowest for meal frequency (meals d(-1)). From periods 1 to 2, all cows showed increases in total daily mealtime, meal frequency, and meal duration; however, cows with lower meal frequencies and feeding intensity in period 1 showed the greatest increases. Cows with high feeding activity and intensity during period 2 showed proportionally greater increases during period 3. These results illustrate that some measures of feeding behavior are highly repeatable within cows, but variable between cows and across stages of lactation. Thus, tests of treatment effects on feeding behavior should be within cow and control for days in milk.     

Short communication: Effects of dietary 5,6-dimethylbenzimidazole supplementation on vitamin B12 supply,lactation performance,and energy balance in dairy cows during the transition period and early lactation

The current study was conducted to investigate the effects of 5,6-dimethylbenzimidazole (DMB) supplementation to the feed during the transition period and early lactation on the vitamin B₁₂ supply, lactation performance, and energy balance in postpartum cows. Twenty-four prepartum Holstein dairy cows were divided into 12 blocks based on their parity and milk yield at the last lactation and were then randomly allocated to 1 of 2 treatments: a basal diet without DMB (control) or a treatment diet that contained 1.5 g of DMB/d per cow. The study started at wk 3 before the expected calving day and ended at wk 8 postpartum. The feed intake and the lactation performance were measured weekly after calving. Blood parameters were measured on d ?10, 0, 8, 15, 29, 43, and 57 relative to the calving day. Body weight was measured on the calving day and on d 57 after calving. The yields of milk, protein, and lactose in cows fed DMB were higher than in the control throughout the whole postpartum stage. On wk 8 postpartum, the vitamin B₁₂ content in the milk and sera was greater in cows fed DMB than in the control. The overall body weight loss from wk 1 to 8 postpartum was less in cows fed DMB than in the control. The plasma content of nonesterified fatty acids and β-hydroxybutyric acid was significantly lower in cows fed DMB than in the control throughout the whole experimental stage. In conclusion, dietary DMB fed during the transition period and early lactation improved the vitamin B₁₂ supply, milk production, and energy balance of postpartum dairy cows.     

Effect of dietary supplements of biotin,intramuscular injections of vitamin B12, or both on postpartum lactation performance in multiparous dairy cows

The current study was conducted to investigate the effects of dietary supplementation of biotin, intramuscular injections of vitamin B₁₂ (VB₁₂), or both beginning at the prepartum period on feed intake and lactation performance in postpartum dairy cows. Forty-eight dairy cows were allocated into 12 blocks, based on parity and milk yield of the previous lactation cycle, and randomly assigned to 1 of 4 treatments. Supplementation of VB₁₂ (weekly intramuscular injections of 0 or 10 mg) and biotin (dietary supplements of 0 or 30 mg/d) were used in a 2 × 2 factorial arrangement in a randomized complete block design of 12 blocks with repeated measures. The study started at 3 wk before the expected calving date and ended at 8 wk after calving. Feed intake and lactation performance (milk yield and composition) were recorded weekly after calving. Blood variables were measured on d ?10, 0, 8, 15, 29, 43, and 57 relative to calving. When VB₁₂ was given, the cows had greater feed intake, better lactation performance and lower body weight loss in the postpartum period compared with animals without injection of VB_12. The VB₁₂-injected cows had lower plasma nonesterified fatty acids and β-hydroxybutyrate concentrations but higher plasma superoxide dismutase activity compared with cows without VB₁₂. Cows fed a biotin supplement had higher milk protein yield (6 and 8 wk) and lactose yield (6–8 wk), compared with animals without biotin. However, under the present experimental conditions, we found no additive effect of a combined supplement of biotin and vitamin B₁₂ on lactation performance of dairy cows.     

Effect of hydroxyselenomethionine on lactation performance,blood profiles,and transfer efficiency in early-lactating dairy cows

The current study investigated the effects of hydroxyselenomethionine (HMBSe), a novel organic selenium (Se) additive, on lactation performance, blood profiles, antioxidative status, and transfer efficiency of Se in early-lactation dairy cows. Sixty multiparous early-lactating dairy cows with similar days in milk (57 d; standard deviation = 9.9) and milk yield (36.5 kg/d; standard deviation = 1.42) were fed a basal diet containing 0.04 mg of Se/kg (dry matter basis). These cows were assigned to 1 of 4 groups following a randomized complete block design as follows: control (basal diet) or HMBSe addition (0.1, 0.3, or 0.5 mg of Se/kg of dry matter). The experiment lasted for 13 wk, with the first week as adaptation. The results showed that milk yields (raw, protein, and lactose) and feed efficiency were improved in a quadratic manner following increased dietary HMBSe addition, whereas energy-corrected milk, 4% fat-corrected milk, and total solid yields tended to be enhanced quadratically. In terms of whole-blood variables, red blood cell and white blood cell levels were increased quadratically, whereas hemoglobin concentration increased linearly with increased HMBSe addition. Plasma nonesterified fatty acid concentrations tended to increase linearly along with HMBSe addition. Plasma superoxide dismutase activity increased quadratically with increased HMBSe addition. The total antioxidant capacity in plasma tended to improve quadratically when cows were fed more HMBSe. Moreover, plasma malondialdehyde concentrations of dairy cows tended to decrease in a quadratic manner when dietary HMBSe increased. The Se concentrations in milk, plasma, and milk/plasma ratio increased linearly following increased HMBSe addition. In conclusion, HMBSe improved lactation performance, health status, and milk Se concentrations in early-lactating dairy cows.     

Effect of vitamin D source and amount on vitamin D status and response to endotoxin challenge

The objectives were to test the effects of dietary vitamin D₃ [cholecalciferol (CHOL)] compared with 25-hydroxyvitamin D₃ [calcidiol (CAL)] on vitamin D status and response to an endotoxin challenge. Forty-five Holstein bull calves (5 ± 2 d of age) were blocked into weekly cohorts, fed a basal diet that provided 0.25 µg/kg body weight (BW) CHOL, and assigned randomly to 1 of 5 treatments: control [(CON) no additional vitamin D], 1.5 µg/kg BW CHOL (CHOL1.5), 3 µg/kg BW CHOL (CHOL3), 1.5 µg/kg BW CAL (CAL1.5), or 3 µg/kg BW CAL (CAL3). Calves were fed milk replacer until weaning at 56 d of age and had ad libitum access to water and starter grain throughout the experiment. Treatments were added daily to the diet of milk replacer until weaning and starter grain after weaning. Measures of growth, dry matter intake, and serum concentrations of vitamin D, Ca, Mg, and P were collected from 0 to 91 d of the experiment. At 91 d of the experiment, calves received an intravenous injection of 0.1 µg/kg BW lipopolysaccharide (LPS). Clinical and physiological responses were measured from 0 to 72 h relative to LPS injection. Data were analyzed with mixed models that included fixed effects of treatment and time, and random effect of block. Orthogonal contrasts evaluated the effects of (1) source (CAL vs. CHOL), (2) dose (1.5 vs. 3.0 µg/kg BW), (3) interaction between source and dose, and (4) supplementation (CON vs. all other treatments) of vitamin D. From 21 to 91 d of the experiment, mean BW of supplemented calves was less compared with CON calves, but the effect was predominantly a result of the CHOL calves, which tended to weigh less than the CAL calves. Supplementing vitamin D increased concentrations of 25-hydroxyvitamin D in serum compared with CON, but the increment from increasing the dose from 1.5 to 3.0 µg/kg BW was greater for CAL compared with CHOL (CON = 18.9, CHOL = 24.7 and 29.6, CAL = 35.6 and 65.7 ± 3.2 ng/mL, respectively). Feeding CAL also increased serum Ca and P compared with CHOL. An interaction between source and dose of treatment was observed for rectal temperature and derivatives of reactive metabolites after LPS challenge because calves receiving CHOL3 and CAL1.5 had lower rectal temperatures and plasma derivatives of reactive metabolites compared with calves receiving CHOL1.5 and CAL3. Supplementing vitamin D increased plasma P concentrations post-LPS challenge compared with CON, but plasma concentrations of Ca, Mg, fatty acids, glucose, β-hydroxybutyrate, haptoglobin, tumor necrosis factor-α, and antioxidant potential did not differ among treatments post-LPS challenge. Last, supplementing vitamin D increased granulocytes as a percentage of blood leukocytes post-LPS challenge compared with CON. Supplementing CAL as a source of vitamin D to dairy calves was more effective at increasing serum 25-hydroxyvitamin D, Ca, and P concentrations compared with feeding CHOL. Supplemental source and dose of vitamin D also influenced responses to the LPS challenge.     

Genetic parameters derived from using a biological model of lactation on records of commercial dairy cows

The object of this study was to investigate the genetics of lactation curve parameters derived from a biological model of lactation and the relationships among them. This biological model fitted 2 logistic curves to mimic the initial increase in milk secretory cell numbers in early lactation and the progression of apoptosis in late lactation. Records from 82,255 Holstein-Friesian heifers from commercial dairy herds in the United Kingdom, recorded from 1994 to 2003, were analyzed. The heritabilities of 2 lactation curve parameters, maximum secretion potential and relative cell death rate, were 0.27 and 0.08 respectively. Maximum secretion potential was highly genetically correlated with peak yield (0.99), and relative cell death rate was highly correlated with persistency of lactation (0.84). Heritability values for the traits analyzed showed a characteristic pattern. Total milk yield traits, maximum secretion potential, and peak yield had similar and moderate heritabilities (∼0.3). Traits associated with late lactation had lower heritability values (∼0.1), whereas day of peak yield and early lactation traits had little genetic variation. The permanent environmental variance of the various traits ranged from 0.08 to 0.26 of the phenotypic variance. Parameters from the 2 logistic curves were not highly correlated, suggesting that selection programs could be devised to exploit genetic variation in both aspects of lactation independently.     

Extended lactation in high-yielding dairy cows. II. Effects on milk production,udder health,and body measurements

The objective of this prospective field study was to evaluate the effects of extending the lactation period of high-yielding dairy cows on milk production, udder health characteristics, and development of body condition. On 40 d in milk (DIM), an examination of the genital tract (transrectal palpation, sonography, vaginoscopy) was performed. Cows without signs of clinical endometritis were blocked by parity and were randomly allocated to 1 of 3 experimental groups with a voluntary waiting period of 40, 120, and 180 d, respectively (G40, n = 135; G120, n = 141; G180, n = 139). Mean daily milk and energy-corrected milk production did not differ between the 3 groups regarding the first 305 d or for the whole lactation (d 1 and up to dry off, culling, or 600 DIM). In late lactation (306 to 600 DIM), G40 had lower average productivity (23.8 kg) compared with G120 (26.5 kg), with G180 showing intermediate values (25.7 kg). The extended lactation groups showed greater persistency, as the rate of decline based on a Wilmink function was lower for G120 (c = ?0.063 and ?0.045 for milk and energy-corrected milk, respectively) and G180 (c = ?0.061 and ?0.047) compared with G40 (c = ?0.071 and ?0.056). We found no difference between the 3 groups regarding the evaluated udder health characteristics (somatic cell count, incidence of mastitis, and days off milk due to mastitis). More cows in G180 (7.9%) were culled due to low productivity compared with G40 (0.7%) and as a tendency compared with G120 (2.8%). Moreover, cows of G180 showed higher median body condition score at the time of dry off compared with cows of both G40 and G120 (3.50 for G180 vs. 3.25 for both G40 and G120). At the time of dry off, G180 cows also had greater backfat thickness (25.0 mm) compared with both G40 (22.2 mm) and G120 cows (21.6 mm). Based on our results, the extension of the voluntary waiting period of high-yielding cows up to 120 d has no adverse effects regarding milk production, involuntary culling, udder health, or BCS gain.