Effect of leaf and spike morphological traits on the relationship between spectral reflectance indices and yield in wheat

While certain spectral reflectance indices have been shown to be sensitive to the expression of a range of performance-related traits in crops, knowledge of the potentially confounding effects associated with plant anatomy could help improve their application in phenotyping. Morphological traits (leaf and spike wax content, leaf and spike orientation, and awns on spikes) were studied in 20 contrasting advanced wheat lines to determine their influence on spectral indices and in their association with grain yield under well-irrigated conditions. Canopy reflectance (400–1100 nm) was determined at heading and grain filling during two growing seasons and three vegetation indices (VIs; red normalized difference vegetation index (RNDVI), green normalized difference vegetation index (GNDVI), and simple ratio (SR)), and five water indices (WIs; one simple WI and four normalized WIs (NWI-1, NWI-2, NWI-3, and NWI-4)) were calculated. The major reflectance fluctuations caused by the differences in leaf and spike morphology mainly occurred in the infrared region (700–1100 nm) and little variation in the visible region (400–700 nm). The NWI-3 ((R₉₇₀ – R₈₈₀)/(R₉₇₀ + R₈₈₀)) consistently showed a stronger association with yield than the RNDVI by using uncorrected canopy reflectance (original raw data) and data adjusted by scattering and smoothing. When canopy reflectance was corrected by a scattering method, the NWI-3 and a modified RNDVI with 958 nm showed the strongest correlations with grain yield by grouping lines for waxy leaves and spikes, curved leaves, and erect and awnless spikes. The results showed that the relationship between the spectral indices and grain yield can be improved (higher correlations) by correcting canopy reflectance for confounding effects associated with differences in leaf and spike morphology.     

Relationships of leaf nitrogen concentration and canopy nitrogen density with spectral features parameters and narrow-band spectral indices calculated from field winter wheat (Triticum aestivum L.) spectra

Remote sensing is a promising tool that provides quantitative and timely information for crop stress detection over large areas. Nitrogen (N) is one of the important nutrient elements influencing grain yield and quality of winter wheat (Triticum aestivum L.). In this study, canopy spectral parameters were evaluated for N status assessment in winter wheat. A winter wheat field experiment with 25 different cultivars was conducted at the China National Experimental Station for Precision Agriculture, Beijing, China. Wheat canopy spectral reflectance over 350–2500 nm at different stages was measured with an ASD FieldSpec Pro 2500 spectrometer (Analytical Spectral Devices, Boulder, CO, USA) fitted with a 25° field of view (FOV) fibre optic adaptor. Thirteen narrow-band spectral indices, three spectral features parameters associated with the absorption bands centred at 670 and 980 nm and another three related to reflectance maximum values located at 560, 920, 1690 and 2230 nm were calculated and correlated with leaf N concentration (LNC) and canopy N density (CND). The results showed that CND was a more sensitive parameter than LNC in response to the variation of canopy-level spectral parameters. The correlation coefficient values between LNC and CND, on the one hand, and narrow-band spectral indices and spectral features parameters, on the other hand, varied with the growth stages of winter wheat, with no predominance of a single spectral parameter as the best variable. The differences in correlation results for the relationships of CND and LNC with narrow-band spectral indices and spectral features parameters decreased with wheat plant developing from Feekes 4.0 to Feekes 11.1. The red edge position (REP) was demonstrated to be a good indicator for winter wheat LNC estimation. The absorption band depth (ABD) normalized to the area of absorption feature (NBD) at 670 nm (NBD670) was the most reliable indicator for winter wheat canopy N status assessment.     

Feasibility of estimating leaf water content using spectral indices from WorldView-3’s near-infrared and shortwave infrared bands

A recently-launched high-resolution commercial satellite, DigitalGlobe’s WorldView-3, has 8 bands in the shortwave infrared (SWIR) wavelength region, which may be capable of estimating canopy water content at 3.7-m spatial resolution. WorldView-3 also has 8 multispectral bands at 1.24-m resolution with two bands in the near-infrared (NIR). The relative spectral response functions for WorldView-3 were provided by DigitalGlobe, Inc., and band reflectances were determined for reflectance spectra of PROSPECT model simulations and leaf data from maize, trees, grasses, and broadleaf herbaceous eudicots. For laboratory measurements, the range of leaf water contents was extended by including drying leaves and leaf stacks of corn, soybean, oaks, and maples. Correlations between leaf water content and spectral indices from model simulations suggested that indices using SWIR band 1 (center wavelength 1210 nm) had low variability with respect to leaf water content, but also low sensitivity. Other indices using SWIR band 5 (2165 nm) had the highest sensitivity, but also had high variability caused by different values of the leaf structure parameter in PROSPECT. Indices using SWIR bands 2, 3 and 4 (1570, 1660, and 1730 nm, respectively) had high correlations and intermediate variability from the leaf structure parameter. Spectral indices calculated from the leaf data had the same overall patterns as the simulations for variation and sensitivity; however, indices using SWIR band 1 had low correlations, and the best correlations were from indices that used SWIR bands 2, 3 and 4. Spectral indices for maize, grasses, and herbaceous crops and weeds had similar responses to leaf water content; tree leaves had higher index values and saturated at lower leaf water contents. The specified width of NIR band 2 (860–1040 nm) overlaps the water absorption feature at 970 nm wavelength; however, the normalized difference of NIR band 1 and 2 was insensitive to water content because NIR band 2’s spectral response was most heavily weighted to wavelengths less than 930 nm. The high spatial resolution of the WorldView-3 SWIR data will help analyze how variation among plant species and functional groups affects spectral responses to differences in canopy water content.     

Discriminant analysis of nitrogen treatments in switchgrass and high biomass sorghum using leaf and canopy-scale reflectance spectroscopy

Recent advances in imaging, laboratory, and field spectroscopy (sometimes referred to as hyperspectral remote sensing) provide a unique opportunity to obtain critical information needed for understanding nitrogen (N) management in crop production systems. Therefore, the objective of this study was to identify wavelength regions and phenological timing useful for the prediction of N status from canopy and leaf spectra. Leaf and canopy spectral data were collected using an ASD FieldSpec FR spectroradiometer (350–2500 nm) at monthly intervals during 2011 and 2012. The crops evaluated in the study were switchgrass ‘Alamo’ (Panicum virgatum L.) and high biomass sorghum ‘Blade 5200’ (Sorghum bicolor) grown to evaluate N applications rates on biomass yield and quality. The optimal wavelengths were determined based on principal component analysis (PCA) and the separation of the N treatments using stepwise discriminant analysis (SDA). The results showed similar canopy and leaf-scale reflectance for high biomass sorghum but not for switchgrass. The wavelengths found to be most important for separating the N treatments were 520–560, 650–690 nm (visible region), and 710–730 nm (red-edge region). Triangular greenness index (TGI) was the most useful index for discriminating the N application rates. The best time for differentiating the different N treatments was 4–6 weeks after planting or 2–4 weeks after N fertilization in high biomass sorghum and within 4 weeks after green-up in switchgrass. In general, the results indicate that spectroscopy is a viable tool that could be used to estimate the biochemical and biophysical characteristics in bioenergy crop production systems.     

Evaluation of nitrogen treatment effects on the reflectance of cotton at different spatial scales

ABSTRACT

The importance of assessing nitrogen (N) status in cotton is important from economic and environmental standpoints. In this study, visible and near-infrared reflectance (NIR) data were collected at cotton leaf-, canopy- and scene-scales at three levels of N treatments to determine the best spatial scale and growth stage that most effectively indicate N treatment effects. While N fertilization affected relative chlorophyll content, leaf area index (LAI), and ground cover (GC) simultaneously, these factors portrayed different effects on cotton reflectance measured at the three spatial scales. Leaf-scale measurement was mainly affected by chlorophyll content. Canopy-scale reflectance was controlled by chlorophyll content and LAI. Scene-scale reflectance was predominantly controlled by GC and to the least extent by chlorophyll content. In terms of visible reflectance, chlorophyll absorption decreased with decreasing N at all spatial scales. Nitrogen treatment effects were most apparent at 550 and 700 nm at the leaf-scale, 610 and 700 nm at the canopy-scale, and 685–690 nm at the scene-scale (after per cent GC exceeded 64%). Only measurements taken at the scene-scale demonstrated a consistent relationship between N fertilization and NIR (800–1000 nm). This information could be useful in the development of N-sensitive indices.     

Nitrogen detection with hyperspectral normalized ratio indices across multiple plant species

The main focus of recent studies relating vegetation leaf chemistry with remotely sensed data is the prediction of chlorophyll and nitrogen content using indices based on a combination of bands from the red and infrared wavelengths. The use of high spectral resolution data offers the opportunity to select the optimal wavebands for predicting plant chemical properties. In order to test the optimal band combinations for predicting nitrogen content, normalized ratio indices were calculated for all wavebands between 350 and 2200 nm for five different species. The correlation between these indices and the nitrogen content of the samples was calculated and compared between species. The results show a strong correlation between individual normalized ratio indices and the nitrogen content for different species. The spectral regions that are most effective for predicting nitrogen content are, for each individual species, different from the normalized difference vegetation index (NDVI) spectral region. By combining the areas of maximum correlation it was possible to determine the optimal spectral regions for predicting leaf nitrogen content across species. In a cross‐species situation, normalized ratio indices using the combination of reflectance at 1770 nm and at 693 nm may give the best relation to nitrogen content for individual species.     

Relationship between spatio-temporal characteristics of leaf-fall phenology and seasonal variations in near surface- and satellite-observed vegetation indices in a cool-temperate deciduous broad-leaved forest in Japan

We examined the relationship between the spatio-temporal distribution of leaf litter for each species and the seasonal patterns of in situ and satellite-observed daily vegetation indices in a cool-temperate deciduous broad-leaved forest. The timing and distribution of leaf-fall revealed spatio-temporal relationships with species and topography. Values of the normalized difference vegetation index (NDVI), enhanced vegetation index (EVI), and green–red vegetation index (GRVI), measured both in situ and by satellite, and those of the in situ-measured leaf area index (LAI), rapidly declined at the peak of leaf-fall. At the late stage of leaf-fall, in situ-measured values of NDVI, EVI, and LAI declined but those of GRVI changed from decreasing to increasing. The peak timing of leaf-fall, when 50–73% of the leaf litter had fallen, corresponds to LAI = 1.80–0.81, NDVI = 0.61–0.54, EVI = 0.29–0.25, and GRVI = 0.01 ～ ?0.07. Although the distribution of leaf litter among species displayed spatial characteristics at the peak of leaf-fall, spatial heterogeneity of amount of leaf litter at the peak timing of leaf-fall was less than that at the beginning and end. These facts suggest that the criterion for determining the timing of leaf-fall from vegetation indices should be a value corresponding to the peak of leaf-fall rather than its end. In a high-biodiversity forest, such as this study forest, the effect of spatial heterogeneity on the timing and patterns of leaf-fall on vegetation indices can be reduced by observing only the seasonal variation in colour on the canopy surface by using GRVI, which consists of visible reflectance bands, rather than that of both leaf area and colour of the canopy surface by using NDVI and EVI, which consist of visible and near-infrared reflectance bands.     

Non-destructive estimation of wheat leaf chlorophyll content from hyperspectral measurements through analytical model inversion

Optimizing nitrogen (N) fertilization in crop production by in-season measurements of crop N status may improve fertilizer N use efficiency. Hyperspectral measurements may be used to assess crop N status indirectly by estimating leaf and canopy chlorophyll content. This study evaluated the ability of the PROSAIL canopy-level reflectance model to predict leaf chlorophyll content of spring wheat (Triticum aestivum L.) during the growth stages between pre-tillering (Zadoks Growth Stage (ZGS 15)) to booting (ZGS50). Spring wheat was grown under different N fertility rates (0–200 kg N ha^?1) in 2002. Canopy reflectance, leaf chlorophyll content, N content and leaf area index (LAI) values were measured. There was a weakly significant trend for the PROSAIL model to over-estimate LAI and under-estimate leaf chlorophyll content. To compensate for this interdependency by the model, a canopy chlorophyll content parameter (the product of leaf chlorophyll content and LAI) was calculated. The estimation accuracy for canopy chlorophyll content was generally low earlier in the growing season. This failure of the PROSAIL model to estimate leaf and canopy variables could be attributed to model sensitivity to canopy architecture. Earlier in the growing season, full canopy closure was not yet achieved, resulting in a non-homogenous canopy and strong soil background interference. The canopy chlorophyll content parameter was predicted more accurately than leaf chlorophyll content alone at booting (ZGS 45). A strong relationship between canopy chlorophyll content and canopy N content at ZGS 45 indicates that the PROSAIL model may be used as a tool to predict wheat N status from canopy reflectance measurements at booting or later.     

Estimating leaf nitrogen concentration in heterogeneous crop plants from hyperspectral reflectance

The estimation of leaf nitrogen concentration (LNC) in crop plants is an effective way to optimize nitrogen fertilizer management and to improve crop yield. The objectives of this study were to (1) analyse the spectral features, (2) explore the spectral indices, and (3) investigate a suitable modelling strategy for estimating the LNC of five species of crop plants (rice (Oryza sativa L.), corn (Zea mays L.), tea (Camellia sinensis), gingili (Sesamum indicum), and soybean (Glycine max)) with laboratory-based visible and near-infrared reflectance spectra (300–2500 nm). A total of 61 leaf samples were collected from five species of crop plant, and their LNC and reflectance spectra were measured in laboratories. The reflectance spectra of plants were reduced to 400–2400 and smoothed using the Savitzky–Golay (SG) smoothing method. The normalized band depth (NBD) values of all bands were calculated from SG-smoothed reflectance spectra, and a successive projections algorithm-based multiple linear regression (SPA-MLR) method was then employed to select the spectral features for five species. The SG-smoothed reflectance spectra were resampled using a spacing interval of 10 nm, and normalized difference spectral index (NDSI) and three-band spectral index (TBSI) were calculated for all wavelength combinations between 400 and 2400 nm. The NDSI and TBSI values were employed to calibrate univariate regression models for each crop species. The leave-one-out cross-validation procedure was used to validate the calibrated regression models. Study results showed that the spectral features for LNC estimation varied among different crop species. TBSI performed better than NDSI in estimating LNC in crop plants. The study results indicated that there was no common optimal TBSI and NDSI for different crop species. Therefore, we suggest that, when monitoring LNC in heterogeneous crop plants with hyperspectral reflectance, it might be appropriate to first classify the data set considering different crop species and then calibrate the model for each species. The method proposed in this study requires further testing with the canopy reflectance and hyperspectral images of heterogeneous crop plants.     

Spectral reflectance and leaf internal structure changes of barley plants due to phytoextraction of zinc and cadmium

The focus of our research is to seek spectral signatures that indicate the impact and content of heavy metals in the leaves and canopies of living plants during the process of phytoremediation. Potted plants of barley (Hordeum vulgare) were grown for 5–6 weeks before being subjected to metal treatments of Zn and Cd. Diffuse reflectance spectra (350–2500 nm) of the plant canopies were collected daily using a portable spectroradiometer throughout the treatment period. Foliar structural changes of Zn‐treated plants included a decrease in intercellular space, palisade and epidermal cell size while Cd‐treated plants displayed fewer structural changes in leaf. Spectral analysis revealed that the band ratios at 1110 nm to that at 810 nm might be used as an indicator of the accumulation of certain metals in plant shoots. Normalized Difference Vegetation Index (NDVI) and leaf‐water‐content indices examined as part of our spectral analysis were not able to distinguish plants treated with different metals. Our ratio index R₁₁₁₀/R_810, on the other hand, correlates closely with the magnitude of leaf structural changes. This study suggests that the infrared reflectance spectrum (800–1300 nm) of plant canopy might provide a non‐intrusive monitoring method for the physiological status of plants grown on heavy metal contaminated soil.     

The effects of phenology on indirect measures of aboveground biomass in annual grasses

Remote sensing is increasingly being used to quantify vegetation biomass across large areas, often with algorithms based on calibrated relationships between biomass and indices such as the normalized difference vegetation index (NDVI). To improve capacity to evaluate grassland dynamics over time, we examined the influence of phenological changes on NDVI–biomass relationships in annual grasses. Our findings support the use of NDVI throughout early growth and the beginning stages of canopy maturation, but suggest caution for later stages. In contrast, measurements of fractional photosynthetically active radiation (fAPAR) absorbed by the canopy and leaf area index (LAI) served as good season‐long surrogates for canopy biomass. Canopies reached maximum biomass approximately 40 days after maximum greenness, with biomass increasing by approximately 20% during senescence. For multi‐year studies of management impact (i) avoid using seasonal comparisons from dates much after the point of maximum greenness or (ii) consider non‐NDVI‐based approaches.     

Evaluating temporal variability in the spectral reflectance response of annual ryegrass to changes in nitrogen applications and leaching fractions

A two‐year study was conducted in 2002 and 2003 at the University of Nevada, Las Vegas's center for urban water conservation to assess canopy spectral response of annual ryegrass (Lolium multiflorum Lam.) grown under various combinations of N and irrigation (based on leaching fraction: LF) treatments. Multispectral measurements were acquired using a ground‐based spectroradiometer (200–1100 nm) on a biweekly basis during the growing season (October–May) in 2002 and 2003. Multispectral parameters were correlated with soil–plant parameters and temporal variability was investigated. Results showed that the normalized difference vegetation index (NDVI), stress index (SI), photochemical reflectance index (PRI) and canopy reflectance at 693 nm, were highly correlated with tissue N concentration (TN), tissue moisture content (TM), TN×TM and canopy colour, as influenced by N and LF treatment combinations. Coefficients of determination ranged from 0.50 to 0.79 (P<0.001) based on single‐day correlations and correlations established over the entire growing period in 2002 and in 2003. TN was mainly predicted from wavelengths in the VIS portion of the spectrum, while TM was predicted from wavelengths in the VIS and NIR. Correlations were inconsistent between spectral parameters and physiological parameters throughout the study confirming the problem of temporal variation associated with spectral signatures of turfgrass species. However, spectral reflectance showed significant potential for monitoring turfgrass N and moisture status, and was able to capture temporal variability over the same growing period and from one year to another. The results provide a sound basis for future validation of ground‐based remote sensing for turfgrass management on golf courses.     

Responses of the reflectance indices PRI and NDVI to experimental warming and drought in European shrublands along a north-south climatic gradient

The aim of this study was to evaluate the use of ground-based canopy reflectance measurements to detect changes in physiology and structure of vegetation in response to experimental warming and drought treatment at six European shrublands located along a North-South climatic gradient. We measured canopy reflectance, effective green leaf area index (green LAIe) and chlorophyll fluorescence of dominant species. The treatment effects on green LAIe varied among sites. We calculated three reflectance indices: photochemical reflectance index PRI [531 nm; 570 nm], normalized difference vegetation index NDVI₆₈₀ [780 nm; 680 nm] using red spectral region, and NDVI₅₇₀ [780 nm; 570 nm] using the same green spectral region as PRI. All three reflectance indices were significantly related to green LAIe and were able to detect changes in shrubland vegetation among treatments. In general warming treatment increased PRI and drought treatment reduced NDVI values. The significant treatment effect on photochemical efficiency of plants detected with PRI could not be detected by fluorescence measurements. However, we found canopy level measured PRI to be very sensitive to soil reflectance properties especially in vegetation areas with low green LAIe. As both soil reflectance and LAI varied between northern and southern sites it is problematic to draw universal conclusions of climate-derived changes in all vegetation types based merely on PRI measurements. We propose that canopy level PRI measurements can be more useful in areas of dense vegetation and dark soils.     

Characterization of the Reflectance Anisotropy of Three Boreal Forest Canopies in Spring–Summer

As a part of the Boreal Ecosystem-Atmosphere Study (BOREAS), measurements of the spectral reflectance anisotropy of three boreal forest canopies were studied for cloudless sky conditions at the phenological growth stages which were at or near maximum leaf area index at each site. The three sites were relatively homogeneous mature stands of black spruce, jack pine, and aspen located in the southern boreal zone of central Saskatchewan. Measurements of the spectal bidirectional reflectance factors with a 15° instrument field of view in three spectral bands centered at 662 nm, 826 nm, and 1658 nm were made with the PARABOLA instrument over a range of solar zenith angles typically varying from 35° (near solar noon) to 70°. The measured reflectance factors showed large anisotropy at all three sites and for all three wavelengths, with prominant backscatter peak reflectances, and strong retro solar view angle (hot spot) maximum reflectances in the visible (662 nm) and shortwave infrared (1658 nm) for the jack pine and black spruce sites, with a less pronounced hot spot at the aspen site. Pronounced effects of canopy and understory shadowing in the visible, as a function of solar zenith angle (SZA), were observed for the black spruce and jack pine sites, with resultant large linear increases in computed normalized difference and simple ratio vegetation indices as SZA increased for near-nadir view angles. Hemispheric spectral reflectances or spectral albedos were computed from angular integration of PARABOLA measured bidirectional reflectances. Visible (662 nm) hemispheric reflectances for the jack pine and black spruce canopies showed very little variation with solar zenith angle, while near-infrared hemispheric reflectances increased strongly with increasing SZA. Estimates were made of the total shortwave albedo for the aspen and jack pine sites from irradiance and reflectance weighting of the spectral hemispheric reflectances in the three measured wavelengths. Comparison of estimated to pyranometer measured total albedo showed all estimates to be biased high, but only by about 0.007–0.018, depending on which of two sets of pyranometer measured albedos were utilized for the comparison. The measured bidirectional reflectance factor (BRF) data sets reported in this study coupled with ancillary data of biophysical parameters collected at the same sites by BOREAS researchers provide a unique data set for the development and characterization of canopy bidirectional reflectance modeling and for the interpretation of remotely sensed data for boreal forest canopies.     

Study of vegetation spectral anomaly behaviour in a porphyry copper mine area based on hyperspectral indices

ABSTRACT

Hyperspectral remote sensing is economical and fast, and it can reveal detailed spectral information of plants. Hence, hyperspectral data are used in this study to analyse the spectral anomaly behaviours of vegetation in porphyry copper mine areas. This analytical method is used to compare the leaf spectra and relative differences among the vegetation indices; then, the correlation coefficients were computed between the soil copper content and vegetation index of Quercus spinosa leaves at both the leaf scale and the canopy scale in the Chundu mine area with different geological backgrounds. Lastly, this study adopts hyperspectral data for the level slicing of vegetation anomalies in the Chundu mine area. The results showed that leaf spectra in the orebody and background area differed greatly, especially in the infrared band (750 nm – 1300 nm); moreover, some indices like the normalized water index (NWI) and normalized difference water index (NDWI) of Quercus spinosa and Lamellosa leaves are sensitive to changes in the geological background. Compared with the canopy, the leaf hyperspectral indices of Quercus spinosa in Chundu can better reflect soil cuprum (Cu) anomaly. In addition, the NWI and NDWI of Quercus spinosa are significantly correlated with the soil Cu content at both the canopy scale and the leaf scale. Consequently, the results of the vegetation anomaly level slicing can adequately reflect the plant anomalies from ore bodies and nearby areas, thereby providing a new ore-finding method for areas with a high degree of vegetation coverage.     

Estimating dry matter content from spectral reflectance for green leaves of different species

Efficient and accurate detection of the temporal dynamics and spatial variations of leaf dry matter content would help monitor key properties and processes in vegetation and the wider ecosystem. However, leaf water content strongly absorbs at shortwave infrared wavelengths, reducing the signal from dry matter. The major objective of this study was to examine relationship between spectral reflectance of fresh leaves and the ratio of leaf dry mass to leaf area, across a wide range of species at the leaf scale. A narrow-band, normalized index combining two distinct wavebands centred at 1649 and 1722 nm achieved the highest overall performance and discriminatory power compared to either single band or first derivatives. The normalized index was evaluated using the PROSPECT (leaf optical properties spectra) simulated reflectance spectra and field measurements from the Leaf Optical Properties Experiment (LOPEX) data set. Both evaluations show that leaf dry matter contents were retrievable with R ² of 0.845 and 0.681 and regression slopes of 0.903 and 0.886. This study suggests that spectral reflectance measurements hold promise for the assessment of dry matter content for green leaves. Further investigation needs to be conducted to evaluate the effectiveness of this normalized index at canopy scales.     

Predicting C3 and C4 grass nutrient variability using in situ canopy reflectance and partial least squares regression

The use of hyperspectral data to estimate forage nutrient content can be a challenging task, considering the multicollinearity problem, which is often caused by high data dimensionality. We predicted some variability in the concentration of limiting nutrients such as nitrogen (N), crude protein (CP), moisture, and non-digestible fibres that constrain the intake rate of herbivores. In situ hyperspectral reflectance measurements were performed at full canopy cover for C3 and C4 grass species in a montane grassland environment. The recorded spectra were resampled to 13 selected band centres of known absorption and/or reflectance features, WorldView-2 band settings, and to 10 nm-wide bandwidths across the 400–2500 nm optical region. The predictive accuracy of the resultant wavebands was assessed using partial least squares regression (PLSR) and an accompanying variable importance (VIP) projection. The results indicated that prediction accuracies ranging from 66% to 32% of the variance in N, CP, moisture, and fibre concentrations can be achieved using the spectral-only information. The red, red-edge, and shortwave infrared (SWIR) wavelength regions were the most sensitive to all nutrient variables, with higher VIP values. Moreover, the PLSR model constructed based on spectra resampled around the 13 preselected band centres yielded the highest sensitivity to the predicted nutrient variables. The results of this study thus suggest that the use of the spectral resampling technique that uses only a few but strategically selected band centres of known absorption or reflectance features is sufficient for forage nutrient estimation.     

Some spectral signatures of Grain Amaranthus (A mar ant has cruentus,L)

Abstract

Ground-based solarimetric measurements on Grain Amaranthus (A. cruentus, L) were made at Akure, Nigeria Latitude 7°N; Longitude 5° E), over a growing season. The solarimeters were fitted with gelatin optical filters (Kodak No. 88A) for measurement in the infrared portion of the solar spectrum. Profile measurements within the canopy were also made at full canopy cover. Simulta neous measurements of the leaf area index (LAI), dry matter production (DM), and photosynthate partitioning (PP) were also made. Results show an increase of infrared reflectance from 0.11 to 0.35 at full canopy cover after which it starts a downward trend. The relations between normalized difference vegetation index (NDVI) and LAI and DM were cubic. It was also observed that the normalised difference was also sensitive to the phenological stage of the crop in that it shows a lower rate of increase when the partitioning of the photosynthates began. Transmitted infrared (IR) radiation within the canopy was observed to increase with depth.     

Seasonal characterization and discrimination of savannah physiognomies in Brazil using hyperspectral metrics from Hyperion/EO-1

The seasonal characterization and discrimination of savannahs in Brazil are still challenging due to the high spatial variability of the vegetation cover and the spectral similarity between some physiognomies. As a preparatory study for future hyperspectral missions that will operate with large swath width and better signal-to-noise ratio than the current orbital sensors, we evaluated six Hyperion images acquired over the Estação Ecológica de Águas Emendadas, a protected area in central Brazil. We studied the seasonal variations in spectral response of the savannah physiognomies and tested their discrimination in the rainy and dry seasons using distinct sets of hyperspectral metrics. Floristic and structural attributes were inventoried in the field. We considered three sets of metrics in the data analysis: the reflectance of 146 Hyperion bands, 22 narrowband vegetation indices (VIs), and 24 absorption band parameters. The VIs were selected to represent vegetation structure, biochemistry, and physiology. The depth, area, width, and asymmetry of the major absorption bands centred at 680 nm (chlorophyll), 980, and 1200 nm (leaf water) and 1700, 2100, and 2300 nm (lignin-cellulose) were calculated on a per-pixel basis using the continuum removal method. Using feature selection and multiple discriminant analysis (MDA), we tested the discriminatory capability of these metrics and of their combined use for vegetation discrimination in the rainy and dry seasons. The results showed that the spectral modifications with seasonality were stronger with the savannah woodland-grassland gradient represented by decreasing tree height, basal area, tree density and biomass and by increasing canopy openness. We observed a reflectance increase in the red, red edge, and shortwave (SWIR) intervals towards the dry season. In the near-infrared, the reflectance differences between the physiognomies were smaller in the dry season than in the rainy season. From the 22 VIs, the visible atmospherically resistant index (VARI), visible green index (VIg), and normalized difference infrared index (NDII) were the most sensitive indices to water stress and vegetation cover, presenting the largest rates of changes between the rainy (March) and dry (August) seasons in shrub and grassland areas. Absorption band parameters associated with the lignin-cellulose spectral features in the SWIR increased towards the dry season with great amounts of non-photosynthetic vegetation (NPV) in the herbaceous stratum. The opposite was observed for the 680 nm chlorophyll absorption band and the 980 and 1200 nm leaf water features. In general, the number of selected metrics necessary for vegetation discrimination was lower in the dry season than in the rainy season. The best MDA-classification accuracy was obtained in the dry season using nine VIs (79.5%). The combination of different hyperspectral metrics increased the classification accuracy to 81.4% in the rainy season and to 84.2% in the dry season. This combination added a gain higher than 10% for the classification of shrub savannah, open woodland savannah and wooded savannah.     

A MODIS-based perpendicular moisture index to retrieve leaf moisture content of forest canopies

Moisture dictates vegetation susceptibility to fire ignition and propagation. Various spectral indices have been proposed for the estimation of equivalent water thickness (EWT), which is defined as the mass of liquid water per unit of leaf surface. However, fire models use live fuel moisture content (LFMC) as a measure of vegetation moisture. LFMC is defined as the ratio of the mass of the liquid water in a leaf over the mass of dry matter, and traditional spectral indices are not as effective as with EWT in capturing LFMC variability. The aim of this research was to explore the potential of the Moderate Resolution Imaging Spectroradiometer (MODIS) on board Terra and Aqua satellites in retrieving LFMC from top of the canopy reflectance, and to develop a new spectral index sensitive to this parameter. All the analyses were based on synthetic canopy spectra constructed by coupling the PROSPECT (leaf optical properties model) and SAIL (Scattering by Arbitrarily Inclined Leaves) radiative transfer models. Simulated top of the canopy spectra were then convolved to MODIS ‘land’ channels 1–7 spectral response functions. All band pairs were evaluated to determine the subspace of MODIS measurements where the separability of points based on their value of LFMC was the highest. This led to the identification of isolines of LFMC in the plane defined by MODIS reflectance measurements in channels 2 and 5; the isolines are straight and parallel, and ordered from lower to higher values of LFMC. This observation allowed the construction of a novel spectral index that is directly related to LFMC – the perpendicular moisture index (PMI). This index measures the distance of a point in the plane spanned by reflectance measurements in MODIS channels 2 and 5 from a reference line, that of completely dry vegetation. Validation against simulated data showed that PMI exhibits a linear relationship with LFMC. When the vegetation cover is dense, the LFMC explains most of the variability in the PMI (R² = 0.70 when LAI > 2; R² = 0.87 when LAI > 4). When the LAI is lower, the contribution of soil background to the measured reflectance increases, and the index underestimates LFMC. The PMI was also validated against the LOPEX93 (Leaf Optical Properties Experiment 1993) data set of leaf optical and biophysical measurements, scaled to canopy reflectance with SAIL, showing acceptable results (R² = 0.56 when LAI > 2; R² = 0.63 when LAI > 4).