响应面法优化黄秋葵热风干燥工艺

为提高黄秋葵干燥效率和质量,通过单因素试验考察黄秋葵的热风干燥温度和风速2个因素对干燥品质的影响,以复水比和色泽为指标,利用响应曲面法研究热风温度、风速和铺料厚度对黄秋葵热风干燥工艺的影响,分析各因素之间的交互作用,获得黄秋葵复水比和色泽的影响特性,建立黄秋葵热风干燥的回归数学模型,确定黄秋葵热风干燥的最优工艺参数。结果表明：黄秋葵热风干燥工艺的最优工艺为热风干燥温度58℃、风速0.62 m/s、铺料层数为1层,所得黄秋葵复水比2.96、色差13.67,试验值与模型预测值吻合度高,误差小于1%。     

响应面法优化烤羊肉工艺参数

主要以新鲜羊后腿肉为原料,利用响应面分析法对风味烤羊肉加工工艺参数优化。通过对烤肉制品的烘烤时间、烘烤温度、肉块大小因素分别进行单因素试验,以烤肉的感官特性为评价指标,并测定新鲜烤肉理化指标以及食用品质,最后得出风味烤羊肉最优加工工艺参数。结果表明:烘烤温度为153℃,烘烤时间为30min,切块厚度为1.6cm时制得的烤羊肉风味最佳。     

响应面法优化腰果脂肪提取工艺

优化腰果脂肪提取工艺。先对提取腰果脂肪的方法进行选择,然后在单因素试验的基础上,应用响应面法对腰果脂肪提取条件进行优化。结果表明,腰果脂肪提取工艺最优条件为料液比1∶27(g/m L),提取时间4.44h,温度60.60℃,提取溶剂为纯石油醚。腰果脂肪实际平均得率为47.76%,所得腰果脂肪提取回归模型高度显著(相关系数R~2=0.984 7),拟合性好(修正后的相关系数R_(Adj)~2=0.965 0)。     

响应面法优化鹰嘴豆蛋白提取工艺

以脱脂鹰嘴豆粉为材料,采用碱溶酸沉法提取鹰嘴豆蛋白,利用单因素试验和响应面法对鹰嘴豆蛋白的提取工艺条件进行分析与优化。结果表明,碱溶酸沉工艺参数对鹰嘴豆蛋白提取率有显著影响,因素影响主次顺序为pH值＞液料比＞提取时间。鹰嘴豆蛋白碱溶酸沉提取优化工艺参数：pH11.0、液料比17.7:1(mL/g)、提取时间88.4min、提取温度20℃,蛋白提取率达到82.33%。所得鹰嘴豆蛋白提取回归模型高度显著(R2＝0.9630),拟合性好,可用于预测蛋白提取率。     

响应面法在生物过程优化中的应用

生物过程优化的目的在于提高终产物的产率,是生物技术产业化不可缺少的一项工作。近年来国内外较为常用的生物过程优化技术包括非统计优化技术和统计优化技术两大类。在生物过程优化中,响应面法是一个更为有效的优化技术。     

响应面在玉米谷蛋白提取中的应用

通过单因素试验研究了碱法提取玉米谷蛋白工艺,采用响应面法对提取时间、水料比和碱液浓度等参数进行了优化.采用多元二次回归方程拟合了三种因素与提取率的关系,预测最佳提取条件为温度50℃,碱浓度0.50%,时间1 h,液固比(V:W)12:1.     

响应面法优化腰果蛋白提取工艺

本实验以海南盛产的腰果为原料,以料液比、提取液p H、提取温度、提取时间为因子,应用单因素及响应面实验优化腰果蛋白碱溶酸沉法提取的工艺条件。结果表明:当料液比为1∶50(g/m L),p H为11,温度为40℃,浸提时间为1.5 h时,腰果蛋白质的提取率最高,可达87.1%±1.05%。所得腰果蛋白提取回归模型高度显著(R~2=0.9406),拟合性好,可用于预测蛋白提取率。      

响应面法优化蚕豆罐头的制作工艺

为开发一款风味好、出品率高的蚕豆罐头,本文以干蚕豆为试验样品,以感官评价为指标,通过单因素试验确定了蚕豆罐头的最佳工艺参数。在此基础上以浸泡水的p H值、浸泡时间和复合磷酸盐添加量为自变量,蚕豆的出品率为因变量,通过响应面法进一步优化蚕豆罐头制作工艺。结果表明,蚕豆罐头的最佳制作工艺为蚕豆在p H值为7.53的水中浸泡47.18 h,罐装时添加0.31%复合磷酸盐,此时蚕豆罐头不仅风味好且出品率高。     

基于响应面法的防水铜版纸原纸生产工艺参数优化

通过响应面法建立了铜版纸原纸3个生产工艺参数:湿强剂、打浆度和松香胶与原纸4个工艺目标紧度、抗张强度、表面吸收质量、光泽度之间的二次回归方程。并对铜版纸原纸生产工艺参数进行了单工艺目标和综合工艺目标优化。优化结果为:湿强剂为1.0%,打浆度为35°S R,松香胶为2.0%;此时,纸张的紧度为0.4822g/cm^3,抗张强度为5.7056k N/m,表面吸收质量为46.133g/cm^2,光泽度为26.781。验证试验表明,实际铜版纸质量指标与优化结果基本一致。     

响应面法优化锅包肉炸制工艺

目的 优化锅包肉炸制工艺。方法 以质构、色差、理化、感官为评价指标, 采用单因素试验考查淀粉种类、初炸温度、初炸时间、复炸温度、复炸时间对锅包肉品质的影响, 通过响应面法优化锅包肉的最佳工艺参数。结果 各影响因素对锅包肉的影响顺序为: 复炸温度>初炸时间>初炸温度>复炸时间, 初炸温度与复炸温度、初炸温度与复炸时间、初炸时间与复炸温度交互作用对感官评分的影响显著。最佳工艺条件为: 选用马铃薯淀粉, 通过初油炸温度175 ℃、初炸时间100 s、复炸温度210 ℃、复炸时间50 s, 在此条件下油炸锅包肉颜色均匀金黄、香气浓郁、软硬适中、具有良好的脆性和感官品质, 感官评分为98.40分。结论 优化后的工艺具有可行性, 可为锅包肉的工业化加工提供一定的理论依据。     

响应面法优化玫瑰醋糟酶解工艺

针对玫瑰醋糟组织致密,高酸性的特点,从水解度的角度出发,通过采用响应面方法对玫瑰醋糟中的大米蛋白提取过程中的温度、酶用量、pH等工艺条件进行了优化.采用Plackett-Burman(PB)设计法,对不同的酶用量、固液比、温度、时间、pH值5个因素对大米蛋白水解度的影响进行评价.结果表明:温度、酶用量、pH值为米糟蛋白提取过程中的主要影响因素,用中心组合设计及响应面分析法确定主要因素的最优条件,为温度56.8℃、酶用量1.1％、pH7.9,得到大米蛋白提取液的水解度为10.04％.     

Expansion characteristics of a nutritious extruded snack food using response surface methodology

Response surface methodology was used to analyze the effect of screw speed (220–340 rpm), feed moisture (11.0–15.0%, wet basis) and feed rate (22.0–26.0 kg/h, wet basis) on the physical properties (i.e., bulk density, expansion, porosity) of a nutritionally balanced extruded snack food. Regression equations describing the effect of each variable on the responses were obtained. Responses were most affected by changes in screw speed followed by feed moisture and feed rate (P<0.05). Expansion and porosity increased with screw speed and feed moisture whereas the opposite was observed for bulk density. Radial expansion was found to be a better index to measure the extent of expansion than the axial and overall expansions, indicated by a higher correlation coefficient.     

真空挤压膨化水酶法提取大豆油的工艺研究

对水酶法提取大豆油真空挤压膨化工艺进行了研究,在单因素试验基础上,采用响应面优化方法确定真空挤压膨化工艺的最优条件为：套筒温度为86.85℃、真空度为-0.067MPa、模孔孔径为22mm、螺杆转速为91r/min、物料含水率为16%,总油提取率可达到93.87%,比传统的湿热预处理后酶解的总油提取率提高了约21个百分点。     

Response surface methodological study on HMF and fluorescent accumulation in red and white grape juices and concentrates

White and red grape juices and their concentrates were subjected to thermal treatments at different temperatures ranging from 50 to 70 °C at different times. 5-Hydroxymethylfurfural (5-HMF) and fluorescence relative index (FLRI) were measured. Response surface methodology (RSM) was applied to determine the effect of temperature and time on the HMF and FLRI formation at the different Brix (bx) degrees. An increase in temperature (from 50 to 70 °C) and time (from 12 to 192 h) for 15°, 45° and 65° Brix degrees was associated with an increase in HMF and FLRI development of white and red grape juices. HMF formation was higher in white grape juice and concentrates than in red ones. Optimum conditions were confirmed and these fitted the experimental data well. Thus, regression equations can be used to estimate HMF and FLRI values at various Brix degrees for white and red grape juices and concentrates.     

Response surface methodology for predicting quality characteristics of beef patties added with flaxseed and tomato paste

Response surface methodology was used to study the effect of flaxseed flour (FS) and tomato paste (TP) addition, from 0 to 10% and 0 to 20% respectively, on beef patty quality characteristics. The assessed quality characteristics were color (L*, a*, and b*), pH and texture profile analysis (TPA). Also, sensory analysis was performed for the assessment of color, juiciness, firmness, and general acceptance. FS addition reduced L* and a* values and decreased weight loss of cooked products (P < 0.05). An opposite effect was observed when TP was added (P < 0.05). All TPA parameters decreased when percentages of FS and TP were increased in the formulation of beef patties. Furthermore, FS and TP addition adversely affected the sensory characteristics of the cooked product (P < 0.05); nevertheless, all sensory characteristics evaluated had an acceptable score (> 5.6). Thus FS and TP are ingredients that can be used in beef patty preparation.     

Response surface methodology for protein extraction optimization of red pepper seed (Capsicum frutescens)

Response surface methodology was used to determine optimum conditions for extraction of protein from red pepper seed meal. A central composite design including independent variables such as temperature (30, 35, 40, 45 and 50 °C), pH (7.0, 7.5, 8.0, 8.5 and 9.0), extraction time (20, 30, 40, 50 and 60 min) and solvent/meal ratio (10:1, 15:1, 20:1, 25:1 and 30:1 v/w) was used. Selected response (dependent variable) which evaluates the extraction process was protein yield and the second-order model obtained for protein yield revealed coefficient of determination of 96.7%. Protein yield was primarily affected by pH and solvent/meal ratio. Maximum yield was obtained when temperature, pH, mixing time and solvent/meal ratio were 31 °C, 8.8, 20 min, 21:1 (v/w), respectively. These conditions resulted in protein yield of 12.24 g of soluble protein from extract/100 g defatted red pepper seed flour. The adequacy of the model was confirmed by extracting the protein under optimum values given by the model. These results help in designing the process of optimal protein extraction from red pepper seeds.     

乙醇浸提菜籽饼中多酚的响应面优化及体外抗氧化性研究

菜籽饼是油菜籽榨油后的主要副产物,其含有较多的多酚类物质,多酚具有一定的抗氧化性,菜籽饼多酚是一种具有开发价值的天然抗氧化剂。本文以菜籽饼为材料,探讨用乙醇浸提多酚的工艺条件及其体外抗氧化性。以多酚得率为考察指标,选择乙醇体积分数、液料比、提取温度和初始pH进行单因素试验,并进行响应面试验确定乙醇浸提菜籽饼多酚的最优工艺条件:乙醇体积分数70%,液料比12∶1,温度52℃、初始pH为3,在此工艺条件下多酚得率为21.96mg/g。以鞣酸、维生素C为对照,通过测定多酚提取液的还原能力、对羟自由基和DPPH.的清除能力,结果表明,多酚提取液具有一定的体外抗氧化能力。     

响应面法优化苋红素提取工艺及其抗氧化性

以彩苋为原料,通过单因素实验,研究提取溶剂、提取时间、提取温度、料液比对苋红素得率的影响,利用响应面法对苋红素得率进行优化,得到最优工艺条件。并对提取出的苋红素粗提液进行抗氧化性研究。结果表明,苋红素得率的最佳提取工艺条件为H₂O作为提取剂,料液比为1∶50（m∶v,g/m L）,提取温度为30℃,提取时间为44 min。在此最优条件下,模型预测提取出的苋红素得率为0.308%,验证实验得到的苋红素得率为0.305%,两者误差为0.98%,证明此工艺条件合理可行。另外,苋红素具有较好的抗氧化能力,对1,1-二硝基苦基苯肼（1,1-diphenyl-2-picrylhydrazyl,DPPH）自由基、羟基自由基（·OH）、超氧阴离子自由基（O₂^-·）的IC₅₀分别为0.18、0.06和0.35 mg/g。      

响应面法优化玉米淀粉液化工艺的研究

以玉米淀粉为试验原料,利用耐高温α-淀粉酶为主要液化酶,依据DE值、折光率两项指标变化情况为衡量指标,采用单因素对比分析与中心组合设计相结合的试验方法,构建玉米淀粉液化技术的三元二次回归模型与逆矩阵分析,获得玉米淀粉液化中最佳工艺参数.研究发现:三元二次多项式回归模型Y=13.62+0.27X1+0.80X2+1.52X3-1.84X12-0.84X22-0.29X32+0.032X1X2-1.55X1X3+1.08X2X3具有显著性(P=0.000 3),决定系数为0.965;当液化温度控制在79.21℃,底物浓度18.38%,加酶量19.98 U/g时,DE值与预测DE值为13.0022接近.     

Colour degradation and quality parameters of sonicated orange juice using response surface methodology

Freshly squeezed orange juice was subjected to sonication at amplitude levels ranging from 40 to 100% at a constant frequency of 20 kHz for different times (2-10 min) and pulse durations of 5 s on and 5 s off. Hunter colour values (L^∗, a^∗ and b^∗), pH, °Brix, titratable acidity, cloud value and browning index were measured. Response surface methodology (RSM) based on two factor five level central composite design was applied to determine the effect of amplitude level and sonication time on juice quality parameters. There was no significant difference (p < 0.05) on pH, °Brix and titratable acidity. The model predictions for critical quality parameters of Hunter colour values (L^∗, a^∗ and b^∗), cloud value and browning index were closely correlated to the experimental results obtained. RSM was demonstrated to be an effective technique to model the effect of sonication on juice quality while minimising the number of experiments required.