Applying technology to operator requirements in medical equipment design

The methodology used by the author consists of the following elements: Expose the design team to the user environment, followed by question and answer periods with users while still in the use environment. Place biomedical engineers in the leading teaching institutions where they will have day-to-day exposure to the use of products similar to the one being designed. Bring biomedical engineers back into the company as part of the design team. Expose concepts to focus groups while the product is in the definition stage. Bring a select group of users into the design review process. Evaluate the ease of use of the device as part of clinical trials. Establish a means of monitoring product performance after the product has been released. How well such a methodology will work in any particular environment is a function of management's recognition of the concept that ease of operator use is a vital element to the overall success of the product.     

系统动态学在区域物流系统研究中的应用

目前,研究区域物流系统的有效方法与手段还比较少,而系统动态学与区域物流系统相结合的研究方法更是一种新的尝试。通过建立区域物流系统动态学模型、对模型的仿真以及对仿真结果的分析,证明用系统动态学方法研究区域物流问题是可行和有效的。     

运用状态检测技术解决装船机的振动问题

针对装船机俯仰机构存在的不正常振动问题,制定了装船机俯仰机构的振动测试方案,确定了测点位置、数量,通过对振动测试数据的分析处理,揭示了俯仰机构不正常振动的根源,提出了解决方案。     

SiLAD:一种蛋白质组学动态分析的新技术

随着蛋白质组学的产生和发展,对蛋白质组表达水平的差异和变化进行体内的和动态的分析已势所必然地成为蛋白质组学研究的发展趋势。但是传统差异蛋白质组学方法只能提供细胞或组织内各种蛋白累积总量的信息,而不是真正意义上的蛋白质差异表达分析。 SiLAD(~(35)S in vivo Labeling Analysis for Dynamic Proteomics)技术正是基于这种需求而在本实验室创立的。SiLAD技术由于其在差异检出的灵敏度和时间分辨率方面的明显优势,以及除提供蛋白质组表达动态以外,可以提供蛋白质代谢等相关动态变化的更多信息,所以可适用于对多种生物过程进行的动态蛋白质组学研究。本文对SiLAD技术的原理及特点进行简要综述。     

Mass spectrometry-based proteomics approaches applied in cataract research

Cataract, the opacification of the eye lens, is the leading cause of blindness worldwide--it accounts for approximately 42% of all cases. The lens fibers have the highest protein content within the body, more than 35% of their wet weight. Given the eye lens pure composition of highly abundant structural proteins crystallins (up to 90%), it seems to be an ideal proteomic entity to study and might be also hypothesized to model the other protein conformational diseases. Crystallins are extremely long-lived, and there is virtually no protein turnover. This provides great opportunities for post-translational modifications (PTM) to occur and to predispose lens to the cataract formation. Despite recent progress in proteomics, the human lens proteome remains largely unknown. Mass spectrometry hold great promise to determine which crystallin modifications lead to a cataract. Quantitative analysis of PTMs at the peptide level with proteomics is a powerful bioanalytical tool for lens-tissue samples, and provides more comprehensive results. New mass spectrometry-based approaches that are being applied to lens research will be highlighted. Finally, the future directions of proteomics cataract research will be outlined.     

SELDI蛋白质芯片技术在蛋白质组学中的应用

本文介绍了SELDI(Suface-enhanced Laser Desorption/Ionization)蛋白质芯片技术的工作原理,讨论了不同类型的蛋白质芯片在蛋白质组学中的应用。     

AB SCIEX TripleTOF 5600在蛋白质组学研究中的应用

AB SCIEX公司最新发布全新的高分辨液相色谱质谱联用系统——TripleTOF5600是在享有盛名的API 5500三重四极杆系统和QSTAR Elite(QqTOF)系统这两个技术平台的基础上开发出来的。TripleTOF 5600高分辨液相色谱质谱联用系统能够对复杂的蛋白质组学样品进行高通量分析,并能同时提供高质量的定性和定量结果。为评估这款仪器的性能,设计基于纳升液相色谱和TripleTOF 5600联用系统的一系列实验。首先,应用这一拥有高分辨率、高灵敏度和高质量准确度的系统对酵母细胞裂解物的胰酶消化产物进行分析。实验中采用每秒最多采集50幅串联质谱谱图的信息相关采集模式,单次LC-MS/MS实验就鉴定10179条独特肽段和1174种蛋白(at 1%global false discoveryrate)。而实际达到平均每秒34幅串联谱图的采集速度是在单次实验获得如此优异的鉴定结果的决定性因素。此外,也对一组含有5种已知蛋白以及部分重同位素标记肽段的样品进行分析,以检验TripleTOF 5600的定量能力。实验中这5种蛋白质都获得很高的序列覆盖率,其中进样量仅有100amol的Peroxiredoxin 1蛋白的序列覆盖率也高达53%。而测试中得到肽段的平均质量偏差仅为1.7 ppm,展示TripleTOF 5600的高质量准确度和稳定性。此外还获得高度准确的定量结果,充分证明TripleTOF 5600能够提供三重四极杆系统级别的定量结果。     

智能化喷雾降尘技术研究

传统的喷雾装置不能有效地降低空气中的粉尘.针对现有生产矿井由于采煤机械化程度提高带来的煤尘增加和具有煤尘爆炸性的特点,设计研究了智能化喷雾,采用感应旋转式装置,当粉尘浓度超过设定值时,粉尘浓度传感器报警.这样既做到了全面除尘又节约了水资源,为工作面创造了良好的、安全的生产环境,有很好的推广价值.     

通用无线分组业务技术在群塔集中管理中的应用

简要概述了加强群塔集中管理的必要性以及通用无线分组业务(general packet radio service,GPRS)的应用现状;通过群塔系统的特点分析,提出基于GPRS的群塔数据无线传输方案,解决了工地监控中心与远程群塔集中管理中心的通信问题;然后从软硬件两方面完成基于GPRS技术的群塔数据传输终端设计,并且对基于GPRS网络的无线数据传输系统进行调试,根据调试结果对软件进行了修改完善,最终实现了数据的可靠传输.     

The application of quantification techniques in proteomics for biomedical research

The systematic analysis of biological processes requires an understanding of the quantitative expression patterns of proteins, their interacting partners and their subcellular localization. This information was formerly difficult to accrue as the relative quantification of proteins relied on antibody‐based methods and other approaches with low throughput. The advent of soft ionization techniques in mass spectrometry plus advances in separation technologies has aligned protein systems biology with messenger RNA, DNA, and microarray technologies to provide data on systems as opposed to singular protein entities. Another aspect of quantitative proteomics that increases its importance for the coming few years is the significant technical developments underway both for high pressure liquid chromatography and mass spectrum devices. Hence, robustness, reproducibility and mass accuracy are still improving with every new generation of instruments. Nonetheless, the methods employed require validation and comparison to design fit for purpose experiments in advanced protein analyses. This review considers the newly developed systematic protein investigation methods and their value from the standpoint that relative or absolute protein quantification is required de rigueur in biomedical research. © 2012 Wiley Periodicals, Inc., Mass Spec Rev 32:1–26, 2013     

航空发动机装配过程仿真技术研究

以航空发动机为研究对象,针对目前装配按照纸质装配工艺规程和二维示意图进行装配不直观的缺点,提出基于三维可视化组件HOOPS实现装配可视化实现的方案,研究了模型获取,装配顺序和路径生成,三维装配动画的表示和动画播放的工步级控制等技术,通过建立装配工艺过程可视化模型,以动画形式来演示航空发动机的装配过程,实现装配过程仿真,以减少人为因素对装配质量的影响.     

无轴传动技术在柔印机上的实现与研究

结合多机组柔印机生产的实际情况,采用无轴传动技术对该设备进行了改造,构造了以SYNA-X200为核心的无轴传动与电气控制系统。实现了纵向套准、裁单张、印刷过程中的张力控制等功能。经过试运行,表明系统套准精度高、张力控制稳定、裁单张误差小、运行可靠。     

Applying the one-column, many pencil local scanning maskless lithography technology to micro-RP system

This paper introduces an innovative one column, many pencil local scanning maskless lithography technology used in dynamic mask and applies it to rapid prototyping (RP) system. By using this technology in micro RP system, the resolution of the 3-D microstructures can be upgraded remarkably. In addition, some relevant experiments are conducted on this micro rapid prototyping system. In the experiment, light beam is projected onto a digital mirror device (DMD) chip [Hornbeck LJ. Digital light processing: A new MEMS-based display, 4th Sensor Symp, p. 297–304 (1996)], and the image is reflected through a micro mirror. Meanwhile, by making use of zoom lens and pins array mask (PAM), the continuing pixels can be scattered. Moreover, in order to project scattered pixels onto the resin surface, the optical system is exploited. Additionally, by using a micro-stage, it is able to control the micro X-Y movement between the scattered pixels. Furthermore, this paper makes use of the spray coating technology to improve the build up capability of the overhand structures of the traditional deep dip liquid-type RP system. Finally, combining the fore-mentioned technologies, one can produce 3-D prototypes with overhand structures in higher resolution.     

用机电一体化技术提高我国刮板输送机制造业水平

分析了工作面刮板输送机的发展趋势，找出了我国刮板输送机制造业在自动化、智能化方面与世界先进水平之间的差距，提出了利用机电一体化技术提高我国煤矿刮板输送机制造水平的关键技术。     

TRIZ理论在飞机结构设计中的应用研究

TRIZ理论是一种创新设计的理论,一种系统的方法学.通过对TRIZ理论的主要内容和工程应用的介绍,结合飞机结构设计的特点和实例分析,表明TRIZ理论可以用于解决飞机结构设计中的问题.针对国内创新设计理论的滞后,引入TRIZ理论,可以适合国内飞机结构设计的现状,在今后的飞机结构设计中将有重要的应用前景.     

航空发动机装配仿真关键技术研究

为解决当前航空发动机装配过程中按照纸质装配工艺规程和二维示意图进行装配不直观的问题,本文提出了基于三维可视化组件HOOPS的装配可视化解决方案,实现了模型获取,装配顺序定义和装配路径生成,三维装配仿真的表示和仿真动画播放的工步级控制等技术,通过建立装配工艺过程可视化模型,以动画形式来演示发动机的装配过程,实现装配过程仿真。     

Recent trends of capillary electrophoresis‐mass spectrometry in proteomics research

Progress in proteomics research has led to a demand for powerful analytical tools with high separation efficiency and sensitivity for confident identification and quantification of proteins, posttranslational modifications, and protein complexes expressed in cells and tissues. This demand has significantly increased interest in capillary electrophoresis‐mass spectrometry (CE‐MS) in the past few years. This review provides highlights of recent advances in CE‐MS for proteomics research, including a short introduction to top‐down mass spectrometry and native mass spectrometry (native MS), as well as a detailed overview of CE methods. Both the potential and limitations of these methods for the analysis of proteins and peptides in synthetic and biological samples and the challenges of CE methods are discussed, along with perspectives about the future direction of CE‐MS. @ 2019 Wiley Periodicals, Inc. Mass Spec Rev 00:1–16, 2019.     

Applying ribbing to plates

Formulas are derived for the upper bounds on the forces and mobility of the material in hot application of ribbing to blanks.     

软氮化新工艺研究

为解决我公司新近订货的软氮化产品硬度偏高的工艺难题,通过借鉴国外先进技术,对我公司现有软氮化设备及工艺进行改进,并取得了良好的成果,不但显著地提高了软氮化硬度,而且拓宽了我公司软氮化的材质范围和订货市场。