酵母提取物对成纤维细胞的作用及抑制黑素生成的研究

通过探索米酒来源酵母提取物(WAE)对成纤维HS68细胞和黑素瘤B16细胞存活率的影响以及对黑素瘤B16细胞黑素生成的作用,研究WAE的安全性及功效性。结果表明,WAE对成纤维HS68细胞和黑素瘤B16细胞的安全质量分数为不高于0.10%;与空白对照组(不含WAE)相比,WAE的质量分数为0.10%和0.01%时,成纤维HS68细胞的存活率分别为106.36%和109.33%,说明WAE在安全质量分数范围内具有促进成纤维HS68细胞增殖的功效;与空白对照组相比,WAE的质量分数为0.10%,0.05%和0.01%时,黑素瘤B16细胞的黑素合成抑制率分别为68.32%,51.68%和19.77%,说明WAE在安全质量分数范围内有抑制黑素瘤B16细胞黑素生成的作用。与相同质量分数的啤酒来源日本酵母提取物(A)和国产酵母提取物(B)相比,WAE的细胞安全性与黑素抑制作用效果更优。     

富锌酵母的研究进展

富锌酵母又称酵母锌或者锌酵母,是在酵母生长发育的过程中向酵母中加入无机锌从而得到富锌能力强的酵母菌株。作为一种补锌的安全原料,富锌酵母在各个方面都有着广泛的应用,把无用的含锌的废弃物利用起来不仅可以充分地节约资源,而且是未来补锌的一种方向。本文主要就富锌酵母的研究进展进行简要综述。     

透明质酸锌的护肤功效研究

以不同分子量透明质酸锌为研究对象,通过斑马鱼保湿、皮脂腺细胞SZ95的脂滴检测、5α还原酶抑制作用、成纤维细胞增殖、Ⅰ型胶原蛋白含量检测、巨噬细胞的炎症因子抑制试验,对其体外保湿、控油、紧致、舒缓功效进行了研究。结果显示0.5%中分子透明质酸锌可以抑制斑马鱼因高渗透压引起的失水,具有显著的保湿功效;大分子透明质酸锌可以通过抑制5α还原酶达到控油功效,中分子、小分子透明质酸锌可以通过抑制脂滴合成、抑制5α还原酶达到控油功效;小分子透明质酸锌有良好的促进成纤维细胞增殖作用,促进作用呈现剂量依赖性,中分子透明质酸锌在质量浓度为125,12.5和1.25μg/mL时,均可显著促进Collagen I的生成;大分子、中分子、小分子透明质酸锌在质量浓度为25,125μg/mL时均可显著抑制炎症因子IL-1α,TNF-α,PGE 2的表达。实验结果表明,透明质酸锌具有良好的保湿、控油、紧致、舒缓的功效。     

混合型水性无机富锌涂料的制备及其性能研究

在片状无机富锌涂料中加入一定比例的球状锌粉,得到性能优异的混合型水性无机富锌涂料,探究了不同锌粉含量下富锌涂料涂层的耐腐蚀性能,通过常规性能测试、耐酸碱性实验对混合型水性无机富锌涂料涂膜进行性能测试。结果表明,当片状锌粉含量为40%、球状锌粉含量为10%时所得到的混合型水性无机富锌涂料性能最优。     

红景天提取物应用于化妆品的生化和细胞学功效评价

通过生物化学和细胞学手段评价红景天提取物的酪氨酸酶抑制、DPPH自由基清除、黑色素抑制、抗衰老等功效。结果表明,红景天提取液体积分数为0.1%、0.5%、1%和5%时,酪氨酸酶抑制率分别为15%、48%、57%和72%;在体积分数为0.125%和0.25%时,红景天提取液的DPPH自由基的清除率达到了71%和86%;倒置显微镜细胞形态观察和黑色素含量检测表明,红景天提取物能够显著地减少小鼠B16和人G361细胞黑色素的生成;同时,红景天提取物能够显著促进1079SK成纤维细胞增殖,提高了18.93%,对皮肤1079SK成纤维细胞UVA光损伤的具有显著的保护作用;0.05 g/L的红景天提取物能够显著促进Ha Cat细胞ATP的形成,ATP相对地增加了19%。这说明,红景天具有美白、抗衰老、抗氧化以及促进皮肤表皮细胞活性等功效,在日用化妆品中有很大的应用和开发价值。     

富硒纤维

<正>富硒纤维是将人体必需的硒、锌等微量元素与纤维素大分子有机结合为一体,制成具有持久缓释有益微量元素的保健功能纤维。河北吉藁化纤有限责任公司的富硒纤维中含有多种人体必需的微量元素,经国家检测部门测试,纤维中含有硒、锌、钠、镁、钾等微量元素,硒的质量分数高达0.9 mg/kg。穿着富硒纤     

添加稀土的富锌铝防腐涂料制备及性能研究

富锌铝防腐涂料通过电化学方式对钢材进行防护,为了提高富锌铝涂料的防腐效果,采用混合树脂为基体,通过在富锌涂料中添加稀土(镧和镨)粉末,制备了含稀土的富锌铝涂料。采用中性盐雾试验测试涂料的防腐效果,电化学极化曲线测试涂层的腐蚀电流和腐蚀电位,扫描电镜(SEM)分析腐蚀后涂层表面的微观形貌,用以研究稀土粉末对富锌铝涂料防腐性能的提高效果。研究表明:在富锌铝涂料中添加适量镧和镨可以代替部分锌粉起到很好的防腐效果,其中镧和镨的掺量分别在0.45%和0.3%时防腐性能最佳。     

燕麦葡聚糖、壳寡糖与中药配伍降血糖的协同作用研究

探讨燕麦葡聚糖、壳寡糖与中药配伍的降血糖作用。选择健康雄性小鼠60只,分为正常对照组、模型对照组及剂量Ⅰ组(燕麦葡聚糖、壳寡糖、富铬酵母)、剂量Ⅱ组(黄精提取物、桑叶提取物、苦瓜提取物、富铬酵母)、剂量Ⅲ组(燕麦葡聚糖、壳寡糖、黄精提取物、桑叶提取物、苦瓜提取物、富铬酵母),每组10只,灌胃给予,实验期30d,测定小鼠体重、空腹血糖值及血糖曲线下面积。结果表明,三个剂量组均具有明显的降血糖作用,且剂量Ⅲ组降血糖作用优于剂量Ⅰ组和剂量Ⅱ组。     

富锌铝防腐涂料的制备及防腐性能研究

为降低富锌涂料中锌粉含量,改善涂料的防腐性能,采用丙烯酸树脂-氨基树脂为基体,超细锌粉和铝粉为防腐填料,配制了富锌和富锌铝涂料。经中性盐雾试验2 500 h后观察其防腐性能差异,同时采用电化学极化曲线测试涂层的腐蚀电流和腐蚀电位变化,采用扫描电镜(SEM)分析腐蚀后涂层表面的微观形貌。研究表明:锌含量76%的富锌涂料防腐性能较差,而在91%的富锌涂料中添加1%~4%铝粉可以代替部分锌粉而起到更好的防腐效果。     

集装箱用环氧富锌涂料防腐性能研究

通过对钢制集装箱用环氧富锌防腐涂料的防腐性能进行研究,制备出具有优异防腐功能且具有优异配套性能环氧富锌涂料。研究了涂膜的锌粉含量、当量比和环氧固化剂种类对环氧富锌防腐涂料耐盐雾性能的影响。结果表明当干涂膜中锌粉含量为82%,且当量比为0.8,使用环氧化合物改性聚酰胺固化剂时,环氧富锌防腐涂料的耐盐雾腐蚀可达1 000 h,配套性能通过IICL认证测试并且海边户外长期防腐性能测试达到5 a。     

从啤酒发酵废水中提取核糖核酸

废酵母是啤酒工业废水中的主要污染物。在常温下,用10%的淡盐水,从废酵母中提取核糖核酸和脱核酵母,回收率为3.3%～3.5%和65.2%～66.5%。对提取液进行预处理后,核糖核酸产品纯度大于90%。提取核糖核酸和脱核酵母后废水的BOD5和CODCr分别下降81%和78%以上。     

Effect of Dieckol, a Component of Ecklonia cava, on the Promotion of Hair Growth

This study was conducted to evaluate the effect of Ecklonia cava, a marine alga native to Jeju Island in Korea, on the promotion of hair growth. When vibrissa follicles were cultured in the presence of E. cava enzymatic extract (which contains more than 35% of dieckol) for 21 days, E. cava enzymatic extract increased hair-fiber length. In addition, after topical application of the 0.5% E. cava enzymatic extract onto the back of C57BL/6 mice, anagen progression of the hair-shaft was induced. The treatment with E. cava enzymatic extract resulted in the proliferation of immortalized vibrissa dermal papilla cells (DPC). Especially, dieckol, among the isolated compounds from the E. cava enzymatic extract, showed activity that increased the proliferation of DPC. When NIH3T3 fibroblasts were treated with the E. cava enzymatic extract and the isolated compounds from the E. cava enzymatic extract, the E. cava enzymatic extract increased the proliferation of NIH3T3 fibroblasts, but the isolated compounds such as eckol, dieckol, phloroglucinol and triphlorethol-A did not affect the proliferation of NIH3T3 fibroblasts. On the other hand, the E. cava enzymatic extract and dieckol significantly inhibited 5α-reductase activity. These results suggest that dieckol from E. cava can stimulate hair growth by the proliferation of DPC and/or the inhibition of 5α-reductase activity.     

Enrichment of antibacterial compound from the stem bark of Stereospermum fimbriatum using supercritical carbon dioxide extraction

ABSTRACT

Supercritical carbon dioxide (sc-CO₂) extraction was carried out on Stereospermum fimbriatum’s stem bark in order to obtain an enriched extract containing the targeted active compound. The recovery of its active compound, C1, was compared with the most active Soxhlet’s extract of dichloromethane. A minimum inhibitory concentration (MIC) assay was done to examine the antibacterial activity of extracts against methicillin-resistant Staphylococcus aureus. The optimum condition for an enriched extract of sc-CO₂ to have a maximum recovery of C1 at MIC value of 400 µg/mL was suggested to be operated at 40°C and pressure at 30 MPa, with addition of 6% co-solvent.     

On‐line LC‐NMR‐MS characterization of sesame oil extracts and assessment of their antioxidant activity

Sesame lignans were isolated by solvent extraction and subsequently purified by solvent crystallization from crude, unroasted sesame oil, and a sesame oil deodorizer distillate. In addition, an aliquot of the purified sesame oil extract was treated with camphorsulfonic acid to obtain a sesaminol‐enriched extract. The sesame lignan composition of the extracts was characterized by on‐line liquid chromatography nuclear magnetic resonance spectroscopy mass spectrometry coupling (LC‐NMR‐MS). The effect of the sesame oil extracts as well as pure sesame lignans and γ‐tocopherol on the oxidative stability of sunflower oil (lignan‐free) was studied by the Rancimat assay. The Rancimat assay revealed the following oxidative stability order: sesame oil extract < sesame oil deodorizer distillate < sunflower oil (no added sesame oil extracts) < sesamol < sesaminol‐enriched sesame oil extract. In addition, the radical‐scavenging capacity of these extracts was assessed by the Trolox® equivalent antioxidant capacity (TEAC) assay. The TEAC assay revealed a slightly different AOX activity order: sesamin < sesame oil extract < sesaminol‐enriched sesame oil extract < sesamol. In conclusion, the sesaminol‐enriched extract revealed strong antioxidant activity and is therefore suitable to increase the oxidative stability of edible oils high in polyunsaturated fatty acids.     

Enrichment of frying oils with plant phenolic extracts to extend the usage life

The aim of this research was to evaluate the efficacy of extracts prepared from olive leaf (OLVL), hazelnut leaf (HAZL), and hazelnut green leafy cover (HGLC) in frying conditions. The extracts were added into canola oil at 200 ppm phenolic equivalence level and fried for seven consecutive days and analyzed. Generally, the lowest phenolic content and antioxidant capacity value were measured in HGLC extract, although, the best performance during frying was with HGLC extract. There were significant differences among the free acidity, conjugated dienoic acids, and total polar materials (TPM). The oil enriched with HGLC extract did not exceed the limit TPM value at the end of seventh day. Also the remaining antioxidant capacities in the frying oil samples were highest in HGLC enriched samples. The viscosity and turbidity values of the oils enriched with OLVL and HAZL extract were a little higher than a control sample. Generally trans‐fatty acid formation was lower in the enriched oil samples. Also significant decreases in the level of unsaturated fatty acids during frying period were observed. This study shows that enriching oils with easily found and cheap natural plant extracts can extend their usage life. Practical applications: The results of this study have shown that liquid frying oils can successfully be enriched with plant phenolic extract to enhance thermo‐oxidative stability. Addition of phenolic extract up to 200 ppm level have not created any problem in sensory quality of either the oil or fried dough. The HGLC and OLVL extract were found very suitable for this purpose. These materials are common agro‐food by‐products and can be produced very easily with low cost. Also, this type of enrichment may aid consumers to get some beneficial phenolic compounds through fried food consumption. In addition, these types of applications may open another area for marketing the named plant extracts.     

Evaluation of In-Vitro Cytotoxicity of Composite Materials Composed of Poly-L-lactic Acid and β-Tricalcium Phosphate

Composite material film composed of Poly-L-lactic acid (PLLA) and β-tricalcium phosphate (β-TCP) was prepared by a solvent evaporation technique. Cellular cultivation in vitro, Von Kossa staining and MTT assay were performed. The results of cytotoxicity test show that cells cultured in extracts of PLLA/β-TCP and on the surface of composite showed normal growth and proliferation, mineralization nodules were observed for fibroblasts cultured in PLLA/β-TCP extract at day 7. Compared with pure PLLA materials, β-TCP in the PLLA composite facilitate both adhesion and proliferation of rat fibroblasts on the PLLA/β-TCP composite film.     

Impact of Canolol‐Enriched Extract from Heat‐Treated Canola Meal to Enhance Oil Quality Parameters in Deep‐Frying: a Comparison with Rosemary Extract and TBHQ‐Fortified Oil Systems

Canolol‐enriched extracts obtained from the extraction of fluidized bed treated canola meal with supercritical carbon dioxide were added to high‐oleic canola oil in different concentrations (200, 500 and 750 mg/kg). After 30 h of deep‐fat frying, oils fortified with canolol‐enriched extracts showed a two to three times better frying performance in comparison to the commonly used antioxidants (TBHQ, 200 mg/kg; rosemary extract, 40 and 200 mg/kg) and a control without antioxidants with regards to the formation of di‐ and polymer triacylglycerols, total polar compounds, secondary degradation products (anisidine value) and the iodine value. The canolol‐enriched extracts were also able to slow down the degradation of α‐ and γ‐tocopherol during frying resulting in significant amounts of tocopherols after 30 h of frying in comparison to the other oils. The influence of the canolol‐enriched extracts indicated strongly concentration‐dependent performance. With increasing concentration of the extract, the thermal stability of the fortified oil was improved. The only disadvantage of the addition of the extracts was an increase in the initial acid value, but within the frying time, only oil fortified with 750 mg canolol‐enriched extract/kg reached the limit given in different countries.     

普洱茶提取物对宫颈癌HeLa细胞的诱导凋亡作用

目的探讨普洱茶提取物对人宫颈癌HeLa细胞系的诱导凋亡作用及其分子机制。方法应用不同浓度的普洱茶提取物(15、30、60、120、250、500μg/ml)作用HeLa细胞后,采用MTT法检测其对细胞增殖的影响;DAPI染色法分析细胞凋亡形态学变化;Annexin V-FITC/PI双染流式细胞术检测细胞早期凋亡;Western blot分析pro-caspase-3和pro-caspase-9的变化。结果普洱茶提取物对HeLa细胞的增殖具有明显的抑制作用,且呈浓度依赖性;经普洱茶提取物处理的细胞荧光显微镜下可见典型的凋亡形态学变化,细胞早期凋亡率显著高于未处理的细胞(P<0.05);经普洱茶提取物处理的细胞pro-caspase-3和pro-caspase-9均被活化。结论普洱茶提取物具有可以诱导HeLa细胞凋亡的天然活性成分,且凋亡途径可能依赖于caspase-3、caspase-9相关的线粒体凋亡途径。     

用埃洛石负载氨基磺酸胍制备阻燃丁腈橡胶

以天然埃洛石纳米管（HNTs）作为载体,通过在其管内负载阻燃剂氨基磺酸胍（GAS）得到功能填料HNTs-GAS,以制备低烟无卤阻燃丁腈橡胶（NBR）。采用热重分析计算得到HNTs与GAS的投料质量比为1/3时可达到质量分数6%的最佳负载率。添加122.3份（质量,下同）HNTs-GAS和5.7份GAS的阻燃NBR复合材料的极限氧指数为28.3%,垂直燃烧达到V-1等级,烟密度为105,具有较好的阻燃性。HNTs-GAS的加入能有效提高NBR的力学性能,与添加相同用量HNTs和GAS的NBR相比,添加HNTs-GAS后NBR复合材料的拉伸强度、撕裂强度和扯断伸长率分别提高了47.5%、36.8%和67.1%。