Effect of chitosan‐propolis edible coatings on stability of refrigerated cachama (Piaractus brachypomus) vacuum‐packed fish fillets

The effect of chitosan‐based coatings containing ethanolic extracts of propolis (EEPs) on the quality of chill‐stored, vacuum‐packed fillets of cachama, an emerging aquaculture species of fish native to the Amazon basins, was evaluated. Coatings were prepared by adding propolis to chitosan following two different strategies: (1) incorporation of microencapsulated EEP and (2) direct incorporation of EEP. Fillets were dip‐coated, vacuum‐packed in medium‐oxygen barrier bags, stored at 4°C, and their quality was evaluated during 20 days, by monitoring lipid oxidation indexes, physicochemical parameters, microbiological quality indicators, texture profile, and sensory acceptance. Chitosan‐EEP coatings significantly increased the time stability of physicochemical, microbiological, and sensory quality indicators of the fish meat compared with control treatments (uncoated vacuum‐packed fillets). In particular, the secondary lipid oxidation of the coated muscles was 12% to 15% lower compared with uncoated samples after 12 days; pH and total volatile base nitrogen (TVB‐N) presented a slower increase in the coated samples; and a greater inhibition of coliforms was observed between days 8 and 16. The chitosan‐EEP coatings had a positive effect in both texture stability and sensory acceptance of the products during storage. Moreover, chitosan coatings with encapsulated EEP had better impact on the evaluated sensory quality parameters. The results indicate that the combination of the barrier properties of chitosan with the bioactive properties of propolis compounds against oxidative free radicals and microbiological deterioration allowed for the edible coatings to extend the shelf life of this aquaculture product with respect to conventionally vacuum‐packed fillets.     

Effect of chitosan coating and vacuum packaging on the quality of refrigerated grilled pork

The effects of chitosan coating and vacuum packaging on the quality and shelf‐life of retail packaged grilled pork in a PVDC/nylon pouch during refrigerated storage at 2 ± 1°C were investigated. Grilled pork without chitosan coating and packaged in 100% air was used as the control sample. Firmness, pH, colour, peroxide values, microbial population and shelf‐life (as determined by sensory characteristics) of the samples were determined during the storage. The results showed that colour, peroxide values and microbial population coincided with sensory characteristics. The delayed growth of microbes in vacuum packaging can be attributed to inhibition by low levels of O₂. Chitosan coating was shown to minimize oxidation, as reflected by the peroxide values, colour changes and microbial counts of the samples. Vacuum‐packaged chitosan‐coated grilled pork showed negligible microbial growth and was found to be organoleptically acceptable throughout the storage period. Chitosan coating along with vacuum packaging provided a type of active packaging to maintain quality and extend the shelf‐life of grilled pork. Copyright © 2006 John Wiley & Sons, Ltd.     

Effect of modified atmosphere packaging and superchilled storage on the shelf‐life of farmed ready‐to‐cook spotted wolf‐fish (Anarhichas minor)

Modified atmosphere packaging (MAP) combined with superchilling (?1°C) was evaluated as a mild preservation method for farmed spotted wolf‐fish (Anarhichas minor). Portions were packaged in air and in CO2:N2 (60%:40%) atmosphere with a gas:product ratio of approximately 1, at superchilled (?1.0°C ± 0.2°C) or chilled (+4.0°C ± 0.2°C) temperatures. A reduced bacterial growth (p < 0.001), measured as aerobic plate counts (APC) and psychrotrophic bacteria, was observed in modified atmosphere (MA) packaged wolf‐fish at both ?1°C and +4°C, compared to portions in air. MA‐packaged wolf‐fish had improved odour and flavour scores (p < 0.05), but also a higher drip loss than fish stored in air. A shelf‐life of 15 days was obtained at ?1°C for MA‐packaged fish compared to 8–10 days in air, and at 4°C the shelf‐life was 13 days in MA and 6–8 days in air. Copyright © 2006 John Wiley & Sons, Ltd.     

Effect of pre-treatment with carbon monoxide and film properties on the quality of vacuum packaging of beef chops

Beef chops (longissimus dorsi) were pre-treated with 5% carbon monoxide (CO) − 95% N₂ for 24 h, vacuum packed in thermo-contractile bags and stored at 0 ± 2°C. Shelf life, as determined by the viable aerobic bacterial load, was 11 weeks. Vacuum-packed chops with heat-contractile film produced a smaller drip loss, had a more intense red colour and higher colour stability under storage than chops with non-heat-contractile film. Chops pre-treated with CO were redder during all the storage period than controls without CO. The pre-treatment did not affect pH, water-holding capacity, drip loss or rancidity of the meat stored in vacuum. Copyright © 2008 John Wiley & Sons, Ltd.     

Effects of PP‐based Nanopackaging on the Overall Quality and Shelf Life of Ready‐to‐eat Salami

A total of 1% nanoclay containing polypropylene (PP)‐nanocomposite and 1% nanoclay plus 5% poly‐beta‐pinene (PβP) containing PP‐active‐nanocomposite materials were produced and tested for packaging of sliced salami. The sliced salami was packaged using both nanofilms and multilayer film of PP/PA/EVOH/PE under vacuum, modified atmosphere packaging under 50% CO₂ and 50% N₂ and air and stored at 4 °C for 90 days. During storage, headspace gas composition; microbial, physical and chemical analyses; and sensory evaluation were performed. The antimicrobial effect of PβP containing nanomaterial was pronounced under vacuum, and no bacterial growth was observed for 75 days. An a* value decreased notably in all applications during storage and preserved best by the multilayered material under vacuum and high CO₂. Thiobarbituric acid reactive substances (TBARS) were 0.63 mg MDA/kg after the processing and gradually increased at all applications during increased storage. TBARS values of all vacuum applications were lower than that of modified atmosphere packaging applications. Rancid taste development was determined by sensory panelists when TBARS values were higher than 0.80 mg MDA/kg on the 90th day. There was no significant moisture loss, and no increase in hardness was determined during the whole storage time. The longest shelf life was 75 days for the sliced salami under vacuum and high CO₂ using the multilayer material. PβP containing nanomaterial provided 50 days of shelf life under vacuum, which is commercially considerable. Copyright © 2017 John Wiley & Sons, Ltd.     

Quality Evaluation of Grated Graviera Cheese Stored at 4 and 12°C using Active and Modified Atmosphere Packaging

The effect of active [oxygen absorber (OA) combined with an ethanol emitter (EE)] and modified atmosphere (100% Nitrogen) packaging in combination with a high barrier experimental polyethylene terephthalate‐silicon oxides//low density polyethelene film on shelf‐life extension of grated Graviera cheese stored at 4 and 12°C was investigated. Microbiological (total viable counts, Pseudomonads, lactic acid bacteria, Enterobacteriaceae and Yeasts/Moulds), physico‐chemical (pH, thiobarbituric acid and colour) and sensory (odour and taste) changes occurring in the product were monitored as a function of treatment and storage time (10 weeks). Sensory shelf‐life was approximately 1, 1.5, 4.5, 6, 9 and at least 10 weeks for control samples (12 and 4°C), for N2 packaged samples (12 and 4°C) and samples packaged with the OA + EE (12 and 4°C), respectively. At the point of sensory rejection, yeasts and moulds increased from 2.00 to 3.60 and 5.55 log CFU/g for control samples stored at 4 and 12°C, respectively. Similarly, for samples stored under nitrogen, yeasts and moulds reached 2.00 and 2.32 log CFU/g at 4 and 12°C, respectively. Yeasts and moulds in samples with the OA + EE remained below 2 log CFU/g throughout the entire storage period, irrespective of storage temperature. pH varied between 5.72 and 6.49 depending on specific treatment. Malondialdehyde absorbance ranged between 0.05 for fresh samples and 0.79 mg/kg at the time of sensory rejection of samples. Light parameters L and b decreased while parameter a increased during storage, reflecting a gradual discolouration of all samples and especially those stored at 12°C.     

Use of Sodium Lactate and Modified Atmosphere Packaging for Extending the Shelf Life of Ready‐to‐Cook Fresh Meal

In this work, the combined effects of sodium lactate and modified atmosphere packaging (MAP) in extending the shelf life of a ready‐to‐cook fresh skewer, made up of raw pork chops and semi‐dried vegetable mix (i.e. zucchini, peppers and tomatoes), were investigated. In the first experimental step, a sodium lactate solution was used to dip pork chops at three different concentrations: 20, 40 and 60% w/w. The second part of the work was focused on the use of MAP. In particular, the following MAPs were tested: MA1 (50%O₂/30%CO₂/20%N₂), MA2 (70%O₂/30%CO₂), MA3 (30%O₂/70%CO₂) and MA4 (30%O₂/30%CO₂/40%N₂). Finally, the optimal concentration of sodium lactate and the best gas composition were combined. The samples were stored at 4 °C; their microbial and sensory qualities were monitored along the entire observation period. The results indicate that the shelf life of the investigated ready‐to‐cook meal can be extended by approximately 83%, if compared with the control skewer packaged in air. The best preservation strategy is the combination of dipping of meat pieces in 40% sodium lactate solution and packaging under MA1. Copyright © 2014 John Wiley & Sons, Ltd.     

Effect of vacuum packaging and sodium acetate on the shelf life of seer fish during iced storage

This paper gives in detail the changes occurring in vacuum‐packed seer fish ( Scomberomorus guttatus ) during ice storage. Seer fish steaks were packed in laminated pouches to study the effect of vacuum packaging with and without sodium acetate treatment, in comparison with air‐packed samples. All these samples were stored in ice and were subjected to biochemical, microbiological and sensory evaluation at regular intervals. The shelf life of seer fish steaks packed without vacuum and treatment was 21 days, whereas sodium acetate‐treated steaks without vacuum and untreated vacuum‐packed steaks had a shelf life of 31 days. Steaks with sodium acetate treatment and vacuum packing had a shelf life of 34 days. Based on the results, it is observed that a combination of vacuum packaging and treatment with sodium acetate significantly increases the storage life of seer fish in ice. Copyright © 2002 John Wiley & Sons, Ltd.     

Modified atmosphere packaging affects lipid oxidation,myofibrillar fragmentation index and eating quality of beef

The objective of this study was to evaluate the effects of different modified atmosphere packaging (MAP) systems applied during ageing and retail display on the quality of beef longissimus dorsi. The quality of the steaks was measured by sensory analysis, thiobarbituric acid‐reactive substances (TBARS), myofibrillar fragmentation index (MFI), protein oxidation, weight loss, cooking loss and vitamin E content. The study included 11 different packaging systems investigated in two separate trials, using O₂, CO₂, N₂ gas and vacuum in combination or alone during ageing and retail display. In some systems, loins were sliced into steaks before packaging, and in other systems, loins were vacuum packed and aged for 14–18 days, and thereafter were sliced and repacked in different atmospheres. Systems including high O₂ MAP resulted in a large increase in warmed‐over flavour and in TBARS as well as a decrease in juiciness, tenderness and vitamin E content, compared to anaerobic systems. Also, MFI was lower in high O₂ MAP steaks, and an increase in protein oxidation was seen, suggesting that the decrease in meat tenderness was due to reduced proteolysis and/or protein oxidation. In addition, the cooked steaks of high O₂ MAP looked well done, although the internal temperature was only 62°C, indicating premature browning. Ageing of steaks in N₂ did not affect any measured parameter compared to vacuum thermoforming packed muscle. Steaks stored in vacuum skin pack for 20 days were less tender than steaks stored in 100% N₂ for 18 days followed by 2 days in atmospheric air. In conclusion, packaging in high O₂ concentrations compromises several aspects of meat quality. Copyright © 2008 John Wiley & Sons, Ltd.     

Effect of Storage in a Low Oxygen Gas Atmosphere on Colour and Sensory Properties of Pork Loins

The objective of this study was to examine the quality characteristics of packaging materials and packaging units, and the viability and acceptability of the colour and sensorial properties of fresh pork meat during storage in low oxygen and high carbon dioxide modified atmosphere packaging up to 16 days at two temperatures (0°C and 4°C), as well as the sensorial properties of cooked meat. Packaging materials had good mechanical and barrier properties and were suitable for packaging in selected atmosphere. The concentration of oxygen during the whole storage period was less than 0.5% at both temperatures. The proportion of red colour (a*) was successfully maintained although the colour was slightly darker (L*) and the sensory value was lower when evaluated at the end of the storage period. Based on the obtained results and discussion, it can be concluded that samples packaged in a modified atmosphere with 40% CO₂ and 60% N₂ can be stored at 0°C for 15 days and at 4°C for 10 days. Samples had a good sensory quality until the 16th day, with the best results achieved on the 7th day. Copyright © 2013 John Wiley & Sons, Ltd.     

高氧气调包装对金针菇保鲜品质的影响

研究了不同气体配比条件对金针菇保鲜效果的影响。 分别用 3% O2 +97% N2 ,80% O2 +20% N2 ,80% O2 +10% N2 +10% CO2 ,80% O2 +20% CO2 气体配比充气包装金针菇,空气包装为对照组。 评定其水分损失率、呼吸强度、色差、相对电导率、多酚氧化酶(PPO)、过氧化物酶(POD)和感官指标等。 结果表明,80% O2 +20% CO2 组水分损失、颜色变化最少,PPO 酶活力、呼吸强度最低,POD 酶活力较高,感官变化最小,贮藏保鲜效果好。     

Combined effect of chill storage and modified atmosphere packaging on mussels (Mytilus galloprovincialis) preservation

Changes in biochemical indices, microbial growth, headspace and sensory quality of mussels which had been packaged in two modified atmospheres [Modified atmosphere packaging (MAP) 1: 60% CO₂/20% N₂/20% O₂ and MAP 2: 60% CO₂/40% N₂] and under vacuum (VP) were studied for 14 days. The results showed better quality retention and greater shelf life of mussels packaged under MAP 1 as compared to MAP 2 and VP samples. Increase in total volatile basic nitrogen followed the order: MAP 1 < MAP 2, VP < air (control) samples while increase in trimethylamine nitrogen followed the order: MAP 1 < air < MAP 2 < VP. The 2‐thiobarbituric acid (TBA) values of MAP 1 and air samples were significantly higher (p < 0.05) than the TBA values of VP and MAP 2 samples. MAP 1 showed a greater (p < 0.05) inhibition effect on total viable count of mussel samples than all other packaging conditions. Based primarily on odour scores, the MAP 1 samples remained acceptable up to ca. 10–11 days, the MAP 2 and VP up to ca. 7–8 days while the air‐packaged samples up to ca. 5–6 days of storage. Copyright © 2007 John Wiley & Sons, Ltd.     

气调包装对冷鲜禽肉感官特性和保质期的影响

目的研究CO组、无氧组、真空组、低氧组、高氧组、普通组等6种包装方法对冷鲜禽肉感官特性和保质期的影响。方法测定禽肉在贮藏不同时间后的感官特性、理化指标(pH值、TVB-N值、TBA值、汁液损失率)和菌落种数的变化。结果随着贮藏时间的增加,各组的感官特性呈现下降趋势,CO组感官指标较好,12 d时保持新鲜;理化指标和菌落总数整体呈上升趋势,CO组、无氧组和真空组上升较慢,保持新鲜9~12 d。结论 CO组、无氧组和真空组保鲜效果较好,保鲜期达12 d,添加了CO(0.5%)对禽肉的感官特性有明显效果,总体保鲜效果为CO组无氧组真空组低氧组高氧组普通组。     

Microbiological,colour and sensory properties of fresh beef steaks in low carbon monoxide concentration

Steaks from gluteus medius m. (GM) and longissimus dorsi m. (LD) were packaged in atmospheres containing two low levels of CO (0.2 and 0.4%) in combination with 21% O₂ or without oxygen. The other components of the modified atmosphere packaging systems were carbon dioxide (30%) and nitrogen (balance). Steaks were evaluated on 0, 7, 14 and 21 days of storage at about 2^oC to investigate the effects of the gas mixtures on the microflora, instrumental (CIE L*a*b*) and visual colour and odour. The preservation of colour and odour of GM and LD steaks packaged under low carbon monoxide was best achieved using anoxic mixtures containing 0.2% CO and 30% CO₂. Steaks packaged without oxygen retained a desirable colour and practically did not present a visible discoloration or perceptive off‐odour along the entire storage time. Colour stabilization with 0.2% CO and 30% CO₂, without O₂, following storage up to 21 days at 2°C did not increase microbial risk of products. The increase in CO from 0.2 to 0.4% had a negative effect on the colour of LD steaks which was regarded as ‘artificial’ by some panellists. The higher stability achieved in this study using 0.2% CO may result from increased stability of the colour from Bos indicus × Bos Taurus cross. Discoloration and off‐odour were evident in the GM and LD steaks packaged aerobically after 14 days of storage when the counts of deteriorant bacteria achieved approximately 6.5 to 9.7 log CFU/g. Highest bacteriostatic effect could be obtained if the volume of injected CO₂ was sufficient to saturate the meat and keep in balance around the product during storage. Copyright © 2010 John Wiley & Sons, Ltd.     

The Effect of Active Packaging on Microbial Stability and Quality of Chinese Steamed Bread

The present study was conducted to evaluate the effect of active packaging [oxygen absorber (OA) or oxygen absorber combined with ethanol emitter (OA + EE)] on microbial stability and quality of Chinese steamed bread (CSB) stored at 25 °C. Air‐packaged CSB was taken as control. Microbiological, sensory, physical, thermal and mechanical changes were monitored as a function of treatment and storage time. On day 2 of storage total plate count was reduced by 3.1, 3.7 and 4.4 cfu/g by OA, OA + EE (1% v/v) and OA + EE (3% v/v), respectively (p < 0.05). Yeasts and moulds were inhibited during the entire storage period. No detrimental effects of OA + EE (1% v/v) were observed on organoleptic parameters, whereas OA + EE (3% v/v) gave a strong objectionable flavour and taste. Active packaging significantly (p < 0.05) delayed the increase of potential of hydrogen as compared to the control. No significant effect of active packaging was found on moisture content, water activity, colour and specific volume. An increase in the melting enthalpy of amylopectin crystallite was observed in all samples during storage. The stored CSB samples packaged with OA showed higher values of hardness compared to samples packaged with OA + EE (1% v/v), indicating the potential of ethanol as plasticizer of the protein network. The results indicate the potential application of OA and OA + EE (1% v/v) as an antimicrobial packaging system for CSB preservation. Copyright © 2015 John Wiley & Sons, Ltd.     

Effect of Pretreatment and Modified Atmosphere Packaging on Quality of dry Coleslaw mix

The aim of the study was to determine the effect of soaking in chlorinated water (100 ppm chlorine) or mild thermal treatment in water (55°C; 3 min) and modified atmosphere packaging on the quality and shelf life of dry coleslaw stored for 12 days at 4°C. Samples were packaged in low‐barrier film in modified atmosphere containing: 5/10/85, 20/25/55, 50/30/20, 70/30/0 %O₂/% CO₂/% N₂, and air atmosphere. In‐pack gas levels, sensory quality, microbial counts and cell permeability were determined. In comparison to non‐processed coleslaw, pretreatment of coleslaw, both consisting in soaking in chlorine solution and a mild thermal processing, resulted in a reduction by approx. 1 logarithmic cycle for counts of psychrophilic and lactic acid bacteria as well as mesophilic and psychrophilic yeasts, by 1 and 1.7 logarithmic cycle for coliform bacteria and by 2 up to 3 logarithmic cycles for Pseudomonas bacteria. Modified atmosphere (irrespective of its composition) used in product packaging was found to have no effect on the count of mesophilic bacteria. An elevated content of carbon dioxide in modified atmosphere (10, 25 or 30%) inhibited growth of psychrophilic bacteria and Pseudomonas bacteria up to 6 days of storage. In the case of coliform bacteria growth inhibition during storage was recorded both in the samples packaged in air atmosphere and in the atmosphere of 50/30/20 and 70/30/0 %O₂/% CO₂/% N₂. In samples packaged in the atmosphere of 70% O₂ and 30% CO₂ after 12 days the greatest cell permeability was recorded, which was connected with a deterioration of their microbiological quality. Copyright © 2015 John Wiley & Sons, Ltd.     

Effect of ovenproof plastic films on the quality of spotted rose snapper (lutjanus guttatus) fillets during frozen storage

Spotted rose snapper (Lutjanus guttatus) fillets packed in two polymer films, polyamide 6,6 (PA66) and polyethylene terephthalate (PET), were stored at ?20°C for a period of 120 days. Films were tested for melting temperature (PA66 253–257°C and PET 252–255°C), oxygen transmission rate [PA66 51.8 ± 12.9cm³/(m²·24h·atm) and PET 152.7 ± 0.2cm³/(m²·24h·atm)] and water vapor transmission rate [PA66 17.8 ± 1.7g/(m²·24h) and PET 6.5 ± 0.2g/(m²·24h)]. Sensory analyses (firmness, flavour and appearance) were performed at 0, 30, 60, 90 and 120 days of storage after baking the fillets in the same storage bags. For raw fillets, their water‐holding capacity (WHC), total aerobic counts (TAC), pH, trimethylamine (TMA) and shear‐force resistance were measured at the same periods. Sensory analyses after baking showed no differences between the fillets packaged in the two films. The WHC, TAC, pH and TMA values in both products were kept within accepted limits. The fillets packed in PET showed a significant increase in firmness (shear‐force resistance) during the first 60 days of storage and then a decrease at 90 days due to dehydration through the film seals. Over the same period, the fillets packed in PA66 showed no significant changes in firmness. In this study, it was demonstrated that it is possible to develop a ready‐to‐bake fish fillet product by using ovenproof films for packaging during storage. Copyright © 2006 John Wiley & Sons, Ltd.     

新鲜竹笋气调保鲜技术的研究

采用O2(2%)+CO2(5%)+N2(93%),O2(80%)+CO2(5%)+N2(15%),O2(80%)+CO2(20%)气调比例包装新鲜竹笋,研究不同气调处理对竹笋保鲜效果的影响。评测竹笋在保存期间水分损失率、L 值、电导率、多酚氧化酶活力、过氧化物酶活力、抗坏血酸、感官品质的变化。结果显示,O2(80%) +CO2(20%) 组水分损失少,L值变化小,电导率上升缓慢,PPO,POD 酶活力低,抗坏血酸含量高,感官品质得分高,贮藏保鲜效果好,贮藏至15d, 仍具有良好的商品品质。     

Modified Atmosphere Packaging of Guava Fruit

Fully matured fresh guava fruits (Lucknow‐49) were precooled at 10 °C for 1 h and pretreated with 500‐ppm benomyl for 5 min. The fruits were packed in 25‐µm and 50‐µm LDPE bags with active modified atmosphere packaging of 3% O₂ + 5% CO₂, 6% O₂ + 5% CO₂ and 9% O₂ + 5% CO₂ gas concentrations and stored at 5 ± 1 °C and 10 ± 1 °C temperatures as well as non‐packaged as control. The shelf life of guava could be increased up to 42 days by packaging in 50‐µm LDPE bags with a gas concentration of 9% O₂ + 5% CO₂ and stored at 10 °C without much change in physical, biochemical and sensory characteristics. Physico‐chemical changes viz., PLW, pulp to peel ratio, firmness, TSS, total sugar, ascorbic acid and titratable acidity were observed minimum for fruits packed in 50‐µm LDPE bags at 3% O₂ + 5% CO₂ gas concentration at 5 °C storage temperature, but sensory score decreased drastically after 21 days of storage. Chilling injury was observed for the fruits packed at 5 °C storage temperature after 21 days of storage, and it was severe in 25‐µm LDPE bag at later stage. Control fruits over‐ripened with the highest weight loss (12.47%) on 6 days of storage and discarded from the storage. Copyright © 2014 John Wiley & Sons, Ltd.     

Effect of Initial Gas Flushing or Vacuum Packaging on the Ripening Dynamics and Preference for Kimchi,a Korean Fermented Vegetable

The effects of vacuum or modified atmosphere packaging conditions on the ripening dynamics of kimchi and sensory preference were investigated to determine the potential of initial atmospheric flushing as a method for improving quality at consumption. In all the packaging treatments (control of air, vacuumizing, CO₂ flushing and N₂ flushing), the partial pressures of O₂ and N₂ remained at nearly constant initial levels; therefore, the increases of package pressures were dominantly caused by the increases in the CO₂ partial pressures as a result of kimchi fermentation. The CO₂ production from kimchi was greater in the vacuumed and CO₂‐flushed packages. The initial vacuumed treatment helped to maintain relatively lower pressure during extended storage, and the pressure of the CO₂‐flushed package could have been reduced as a result of the high solubility of CO₂ in the aqueous product. Initial CO₂ flushing also provided the packaged product with a pleasant cool flavour at the properly ripened state.