共查询到20条相似文献,搜索用时 93 毫秒
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通过天然阳离子型高分子壳聚糖与三聚磷酸钠的静电自组装,制备模仿细胞外基质纳米结构的仿生壳聚糖基纳米纤维。研究了介质、壳聚糖浓度、反应物比例、添加胶原等因素对自组装复合产物的影响;并采用透射电镜、X射线衍射和红外光谱等技术表征了纳米纤维的结构。结果表明,介质对壳聚糖基纳米复合物的结构和形态的影响很大;以己二酸溶液为介质,在温和条件下,可获得结构可控的壳聚糖基纳米纤维,有望用于构建模仿天然细胞外基质的组织工程支架。 相似文献
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曾戎刘宏伟屠美赵剑豪查振刚周长忍 《高分子材料科学与工程》2009,(2):122-125
通过天然阳离子型高分子壳聚糖与三聚磷酸钠的静电自组装,制备模仿细胞外基质纳米结构的仿生壳聚糖基纳米纤维。研究了介质、壳聚糖浓度、反应物比例、添加胶原等因素对自组装复合产物的影响;并采用透射电镜、X射线衍射和红外光谱等技术表征了纳米纤维的结构。结果表明,介质对壳聚糖基纳米复合物的结构和形态的影响很大;以己二酸溶液为介质,在温和条件下,可获得结构可控的壳聚糖基纳米纤维,有望用于构建模仿天然细胞外基质的组织工程支架。 相似文献
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《高分子材料科学与工程》2021,37(8)
为探讨多糖类生物质材料的高效利用,文中以纤维素硫酸钠-氯化壳聚糖-三聚磷酸钠(NaCS-CHC-STPP)微胶囊为核心/模板,以1%NaCS和0.5%CHC作为自组装的聚电解质溶液,采用层层自组装法制备了具有4层膜层结构的PEC_4微胶囊,探究了自组装膜层结构对微胶囊的缓释性能影响。通过数码单反相机、扫描电子显微镜、Zeta电位分析等对PEC微胶囊的膜层结构进行了表征,并在模拟结肠液中对载药微胶囊的释放性能进行了测定与分析。结果表明,与模板相比(n=0),经层层自组装后得到的微胶囊形貌圆润,内部结构更加致密(1≤n≤3)。Zeta电位分析表明,随NaCS与CHC的交替组装,电位呈现规律性变化。傅里叶变换红外分析表明CHC与NaCS发生了化学反应。释放研究表明,随着自组装PEC膜层结构的增加(1≤n≤2),载药微胶囊的缓释给药效果得到明显提升,释放过程中微胶囊逐渐变褶皱、粗糙,伴有微孔结构。该研究结果可以为生物质材料构建缓释型药物递送体系提供理论参考。 相似文献
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金纳米粒子以它独特的光学、电学和催化性质以及在纳米级电子线路中的应用潜力,受到人们越来越多的关注.本文主要评述了金纳米粒子的合成方法和自组装技术,即对各种制备方法和自组装的特点、纳米粒子的生长机理和自组装机理进行了介绍.展望了金纳米材料未来的研究方向和发展趋势. 相似文献
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通过小分子十二烷基硫酸钠(SDS)调节牛血清白蛋白(BSA)疏水区域暴露,使白蛋白分子在不借助额外催化剂和交联剂的相对温和条件下自组装,合成了一种可共载光敏剂二氢卟吩e6(Ce6)及基因(CpG寡脱氧核苷酸)的白蛋白纳米载体。在其成功负载光敏剂Ce6后,用聚醚酰亚胺(PEI)对BSA纳米颗粒(BSA NP)表面进行了修饰,并通过调节合适的修饰比例得到高效负载基因的纳米体系。随后对纳米颗粒体系的形貌、尺寸分布、表面电荷、稳定性以及基因负载能力、产生活性氧能力进行了初步评估。结果表明合成得到的PEI修饰的白蛋白共负载Ce6及CpG(BSA-Ce6-PEI-CpG)的纳米颗粒尺寸分布均匀,微观形貌呈现光滑规整的圆球形,对CpG基因有较好的负载能力,并在光照下高效产生活性氧,且纳米体系能够在表面活性剂处理后依然保持结构稳定,可作为良好的光敏剂和基因递送载体。 相似文献
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采用常压干燥法制备了SiO2气凝胶,所得气凝胶为介孔结构,比表面积618.8m2/g,孔径分布5~20nm.以SiO2气凝胶为原料,通过静电吸附法制备了SiO2气凝胶/壳聚糖复合药物载体材料,采用扫描电镜(SEM)、红外光谱(FTIR)等对复合材料的结构形貌进行了分析,研究了复合材料对硫酸庆大霉素药物的担载和释放性能.结果表明,所得SiO2气凝胶/壳聚糖复合材料为多孔网络结构,其中,由450℃处理的SiO2气凝胶制得的气凝胶/壳聚糖复合材料对硫酸庆大霉素具有较好的药物担载和缓释性能. 相似文献
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A novel targeted drug delivery system, glucose-conjugated chitosan nanoparticles (GCNPs), was developed for specific recognition and interaction with glucose transporters (Gluts) over-expressed by tumor cells. GC was synthesized by using succinic acid as a linker between glucosamine and chitosan (CS), and successful synthesis was confirmed by NMR and elemental analysis. GCNPs were prepared by ionic crosslinking method, and characterized in terms of morphology, size, and zeta potential. The optimally prepared nanoparticles showed spherical shapes with an average particle size of (187.9 ± 3.8) nm and a zeta potential of (-15.43 ± 0.31) mV. The GCNPs showed negligible cytotoxicity to mouse embryo fibroblast and 4T1 cells. Doxorubicin (DOX) could be efficiently entrapped into GCNPs, with a loading capacity and encapsulation efficiency of 20.11% and 64.81%, respectively. DOX-Ioaded nanoparticles exhibited sustained-release behavior in phosphate buffered saline (pH 7.4). In vitro cellular uptake studies showed that the GCNPs had better endocytosis ability than CSNPs, and the antitumor activity of DOX/GCNPs was 4-5 times effectiveness in 4T1 cell killing than that of DOX/CSNPs. All the results demonstrate that nanoparticles decorated with glucose have specific interactions with cancer cells via the recognition between glucose and Gluts. Therefore, Gluts-targeted GCNPs may be promising delivery agents in cancer therapies. 相似文献
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Chung JW Lee K Neikirk C Nelson CM Priestley RD 《Small (Weinheim an der Bergstrasse, Germany)》2012,8(11):1693-1700
The ability to create aqueous suspended stable nanoparticles of the hydrophobic homopolymer poly(?‐caprolactone) end‐functionalized with coumarin moieties (CPCL) is demonstrated. Nanoparticles of CPCL are prepared in a continuous manner using nanoprecipitation. The resulting nanoparticles are spherical in morphology, about 40 nm in diameter, and possess a narrow size distribution and excellent stability over 4 months by repulsive surface charge. Nanoparticle size can be easily controlled by manipulating the concentration of CPCL in the solution. The interparticle assembly between the nanoparticles can be reversibly adjusted with photoirradiation due to photoinduced [2 + 2] cyclodimerization and cleavage between the coumarin molecules. In addition, the CPCL nanoparticles show significant cellular uptake without cytotoxicity, and the intrinsic fluorescence of the coumarin functional group permits the direct detection of cellular internalization. 相似文献
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Sarah Baltzley Azzam A. Malkawi Motasem Alsmadi 《Drug development and industrial pharmacy》2018,44(9):1467-1472
Introduction: The aim of this study was to investigate ketorolac (KT) systemic absolute bioavailability after sublingual (SL) administration in vivo to conscious rabbits. Furthermore, the study investigated the potential use of chitosan nanoparticles as a delivery system to enhance the systemic bioavailability of KT following SL administration.Methods: Ketorolac-loaded chitosan nanoparticles were prepared through ionotropic gelation of chitosan with tripolyphosphate anions. The KT-nanoparticles were administered SL as a spray to rabbits and KT plasma concentration at predetermined time points was compared to SL spray administration of KT in solution. The concentrations of KT in plasma were analyzed by ultra-performance liquid chromatography mass spectroscopy (UPLC/MS).Results: KT-loaded chitosan nanoparticles significantly (p?.05) enhanced systemic absorption with 97% absolute bioavailability as compared to 70% after SL administration of KT solution.Conclusions: The results of the present study suggest that SL absorption of KT illustrated flip-flop kinetics with prolonged persistence in the body compared to intravenous administration. Formulation of KT as chitosan nanoparticles has increased its systemic bioavailability after SL spray administration. The new delivery system could be an attractive approach for the delivery of KT. 相似文献
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聚乳酸(PLA)作为药物载体材料存在因疏水性强而导致的药物释放速率难控以及在循环系统中停留时间短等问题.研究表明,在PLA中引入乙醇酸(GA)可提高材料降解速率,引入聚乙二醇(PEG)则可延长共聚物在循环系统中的停留时间.研究以丙交酯(LA)和对二氧环己酮(PDO)为主要原料,在辛酸亚锡-乙二醇共引发体系的存在下,通过熔融开环聚合制备出了端羟基聚(丙交酯-co-对二氧环己酮)(HO-P(LA-co-PDO)-OH).这种同时具有PLA、GA和EG结构单元的大分子二醇可望成为一种降解速率可控、在循环系统中停留时间可调的新型药物载体材料.采用DSC、~1H NMR、~(13)C NMR和GPC-MALLs等对其结构和热学性能进行了表征.分子量检测结果表明,HO-P(LA-co-PDO)-OH的分子量随原料中PDO/LA摩尔比的减小而增大. 相似文献
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含纳米银的明胶/壳聚糖纳米纤维的制备及其抗菌性能研究 总被引:2,自引:0,他引:2
以浓度为88%的甲酸溶液作为纺丝溶剂,采用静电纺丝和紫外光照射还原的方法制备了含纳米银颗粒的明胶/壳聚糖纳米纤维。研究发现,壳聚糖的加入量低于明胶质量的3/16时可以得到纳米纤维,纤维平均直径随着硝酸银加入量的增大而减小,纤维表面纳米银的平均直径随着硝酸银加入量的增大而增大,在纺丝体系中硝酸银的加入量存在一个极限值。所制得含纳米银的明胶/壳聚糖纳米纤维对金黄色葡萄球菌和绿脓杆菌具有较好的抑菌性能,纺丝时加入1%硝酸银制得纳米纤维膜的抑菌率达到99%以上,这种抗菌型纳米纤维可以应用于医用敷料等领域。 相似文献
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Objective: In this study, a dimple-shaped chitosan carrier was developed for delivering the antituberculosis drug ethambutol dihydrochloride (EDH) from a dry powder inhaler (DPI) to the lungs.Materials and methods: Nanosized drug particles were prepared using nanospray drying. The microsized carrier was developed from a chitosan solution by spray drying. Five formulations were prepared by physically mixing the drug and carrier in different ratios. The physico-chemical properties of the formulations were analyzed using scanning electron microscopy, differential scanning calorimetry, Fourier transform infrared spectroscopy, ultracentrifugation and a cascade impactor.Result and discussion: The EDH size was 222?nm and the chitosan carrier size was 1.2?µm. Five formulations, i.e. 1:2, 1:2.5, 1:3.3, 1:5 and 1:10 w/w of the EDH to chitosan carrier were prepared by physical mixing. The chitosan carrier was spherical in shape with a dimpled surface and this provided shallow cavities to which the drug was bound, both within its grooves as well as on its surface. The median adhesion force (50% of drug detachment) for formulations #1 to #5 was between 122 and 993?µN. The mass median aerodynamic diameter of the EDH was between 2.3 and 2.7?μm, with the fine particle fractions (aerosolized particles less than 4.4?μm) of 32–42% of the nominal dose.Conclusion: We suggest that ethambutol dihydrochloride mixed with a chitosan carrier was suitable for use in a dry powder inhaler for controlling tuberculosis especially in minimizing the risk of multidrug resistant tuberculosis and the possible side effects from EDH. 相似文献