表面微观形貌的显微干涉检测原理及干涉显微镜发展现状

追踪分析世界上表面微观形貌检测方面显微干涉检测原理的最新进展 ,比较干涉显微镜用于检测表面微观形貌时具有的形式、结构特点 ,分析选型研制干涉显微镜可能遇到的问题及应该研究的方面。     

光干涉法显微镜测量三维表面形貌研究

本文介绍了利用光干涉法显微镜测量三维表面形貌.利用计算机对在光干涉法显微镜下拍摄的零件表面轮廓条纹图象进行图象识别与处理,自动寻找条纹和计算,直接获得三维表面轮廓形貌.测量结果与触针式轮廓仪的测量结果进行了比较.     

基于微分干涉相衬的相位分析法研究

通过对微分干涉相衬显微定量测量方法进行研究,提出了一种更有效的相位分析法。即在不对双光束干涉光路进行改造或处理的前提下,通过对光学成像进行处理而得到理想的结果。即把图像中的光强信号转变成相位信号,并通过维纳滤波对噪声进行了消除,最后获得表面微观形貌定量参数。     

干涉法表面形貌测量使用的扩展深度测量范围的方法

系统归纳了在干涉法表面形貌测量中使用的用来扩展深度测量范围的各种方法,分析了这些方法的原理、特性及性能指标,比较了这些方法的优越性。     

旋转检偏器移相的微分干涉显微镜

提出了一种干涉相位差与检偏器方位角成线性关系的新型微分干涉显微镜,利用相移干涉技术,可定量测量样品的表面形貌及粗糙度参数,系统的垂直和横向分辨率分别为1nm和0.5μm。     

光学元件形貌的双波长干涉测量

本文提出了一种利用双波长激光干涉产生交叉的干涉条纹测量光学元件三维面形的方法.该方法可以有效地提取干涉图像中更多的数据点,恢复被测元件的三维形貌.一定程度上解决了大口径光学元件三维形貌测量中数据点密度不足的问题.     

用干涉显微镜测量离子镀反射薄膜的厚度

本文指出,用干涉显微镜可以测量离子铰薄膜的厚度。本文介绍了测量的方法,并说明这种方法的优点。     

Linear phase imaging using differential interference contrast microscopy

We propose an extension to Nomarski differential interference contrast microscopy that enables isotropic linear phase imaging. The method combines phase shifting, two directions of shear and Fourier‐space integration using a modified spiral phase transform. We simulated the method using a phantom object with spatially varying amplitude and phase. Simulated results show good agreement between the final phase image and the object phase, and demonstrate resistance to imaging noise.     

Scanning differential interference contrast microscope

An optical microscope has been developed based on the differential interference contrast method to evaluate the roughness of supersmooth surfaces. The instrument uses a Bragg cell and a translation stage driven by a DC motor to produce an image of an area of a sample. Its lateral resolution is 2 μm and its vertical resolution is subangstrom. It takes only 7 seconds to scan an area of 1 mm². Three different curve fits can be used to remove the tilt, the curvature, and the low spatial frequency features of the sample. A figure for surface roughness is produced that is repeatable to 0.01 Å. The instrument is described and the noise sources and repeatability are discussed. The results of measurements of ring laser gyroscope mirror substrates are shown.     

Quantitative phase-amplitude microscopy II: differential interference contrast imaging for biological TEM

Although phase contrast microscopy is widespread in optical microscopy, it has not been as widely adopted in transmission electron microscopy (TEM), which has therefore to a large extent relied on staining techniques to yield sufficient contrast. Those methods of phase contrast that are used in biological electron microscopy have been limited by factors such as the need for small phase shifts in very thin samples, the requirement for difficult experimental conditions, or the use of complex data analysis methods. We here demonstrate a simple method for quantitative TEM phase microscopy that is suitable for large phase shifts and requires only two images. We present a TEM phase image of unstained Radula sp. (liverwort spore). We show how the image may be transformed into the differential interference contrast image format familiar from optical microscopy. The phase images contain features not visible with the other imaging modalities. The resulting technique should permit phase contrast TEM to be performed almost as readily as phase contrast optical microscopy.     

Variable multimodal light microscopy with interference contrast and phase contrast; dark or bright field

Using the optical methods described, specimens can be observed with modified multimodal light microscopes based on interference contrast combined with phase contrast, dark‐ or bright‐field illumination. Thus, the particular visual information associated with interference and phase contrast, dark‐ and bright‐field illumination is joined in real‐time composite images appearing in enhanced clarity and purified from typical artefacts, which are apparent in standard phase contrast and dark‐field illumination. In particular, haloing and shade‐off are absent or significantly reduced as well as marginal blooming and scattering. The background brightness and thus the range of contrast can be continuously modulated and variable transitions can be achieved between interference contrast and complementary illumination techniques. The methods reported should be of general interest for all disciplines using phase and interference contrast microscopy, especially in biology and medicine, and also in material sciences when implemented in vertical illuminators.     

Confocal differential interference contrast (DIC) microscopy: including a theoretical analysis of conventional and confocal DIC imaging

A technique for obtaining differential interference contrast (DIC) imaging using a confocal microscope system is examined and its features compared to those of existing confocal differential phase contrast (DPC) techniques as well as to conventional Nomarski DIC. A theoretical treatment of DIC imaging is presented, which takes into account the vignetting effect caused by the finite size of the lens pupils. This facilitates the making of quantitative measurements in DIC and allows the user to identify and select the most appropriate system parameters, such as the bias retardation and lateral shear of the Wollaston prism.     

Phase‐shifting differential interference contrast microscope with Savart shear prism and rotatable analyser

A novel differential interference contrast microscope (DICM) is proposed in this research. It is constituted by inserting a Savart shear prism between the objective and sample of a polarising microscope having a rotatable analyser as the phase‐shifter, and it is with the ability to enhance image contrast using the principle of shearing interferometry. This letter is to introduce the configuration, interpret the interference patterns and present the experimental setup of the DICM. In addition, this letter is to display the experimental results from the uses of the setup; the results demonstrate the validity and ability of the DICM.     

Real‐time three‐dimensional imaging of cell division by differential interference contrast microscopy

Differential interference contrast (DIC) microscopy can provide information about subcellular components and organelles inside living cells. Applicability to date, however, has been limited to 2D imaging. Unfortunately, understanding of cellular dynamics is difficult to extract from these single optical sections. We demonstrate here that 3D differential interference contrast microscopy has sub‐diffraction limit resolution both laterally and vertically, and can be used for following Madin Darby canine kidney cell division process in real time. This is made possible by optimization of the microscope optics and by incorporating computer‐controlled vertical scanning of the microscope stage.     

A hybrid scanning force and light microscope for surface imaging and three-dimensional optical sectioning in differential interference contrast

The design of a scanned-cantilever-type force microscope is presented which is fully integrated into an inverted high-resolution video-enhanced light microscope. This set-up allows us to acquire thin optical sections in differential interference contrast (DIC) or polarization while the force microscope is in place. Such a hybrid microscope provides a unique platform to study how cell surface properties determine, or are affected by, the three-dimensional dynamic organization inside the living cell. The hybrid microscope presented in this paper has proven reliable and versatile for biological applications. It is the only instrument that can image a specimen by force microscopy and high-power DIC without having either to translate the specimen or to remove the force microscope. Adaptation of the design features could greatly enhance the suitability of other force microscopes for biological work.     

Edge detection,three-dimensional cell boundary reconstruction and volume and surface area estimation from differential interference contrast images

Three-dimensional (3-D) cell morphology is important for the understanding of cell function and can by quantified in terms of volume and surface area. Differential interference contrast (DIC, or Nomarski) imaging can enable cell edges to be clearly visualized in unstained tissue due to the slight difference in refractive index between aqueous media and cytoplasm. DIC is affected in only one direction - the direction of the optical shear. A 1-D edge detector was used in that direction with a scale length equal to that of an in-focus edge to highlight cell boundaries. By comparison with the signal from the edge detector on an out-of-focus slice, the in-focus slices could be segmented and, after noise suppression, cell outlines obtained. A voxel paradigm was used to calculate cell volume and differential geometry was used for surface area estimation. We applied this approach to obtain 3-D dimensional information by optical sectioning of motile Amoeba proteus.