共查询到20条相似文献,搜索用时 31 毫秒
1.
Robert W. Lencki Natasja Smink Heleen Snelting Joseph Arul 《Journal of the American Oil Chemists' Society》1998,75(9):1195-1200
Factors affecting the release of short-chain fatty acids during hydrolysis of a butterfat fraction with a 1,3-positional and
short-chain-specific Penicillium roqueforti lipase were investigated. When a short-chain triglyceride fraction was used as substrate, as opposed to whole butterfat,
the ratio of desirable flavor short-chain free fatty acids (FFA) to undesirable medium-chain FFA in the FFA fraction increased
from 0.75 to 1.80. However, with both substrates, FFA accumulation eventually led to lipase inhibition and limited the total
amount of triglyceride hydrolysis. This inhibition phenomenon was principally due to product inhibition. Periodically extracting
the FFA with a buffer solution minimized this inhibition phenomenon, thereby significantly increasing lipase activity and
the degree of triglyceride hydrolysis. Thus, on-line extraction of FFA in lipase reactors has the potential of greatly increasing
system productivity. 相似文献
2.
Gerald P. McNeill Philip E. Sonnet 《Journal of the American Oil Chemists' Society》1995,72(2):213-218
Three lipases were compared for their ability to hydrolyze high erucic acid rapeseed oil, with the objective of concentrating
the erucic acid in a single glyceride fraction. Lipase fromPseudomonas cepacia released all fatty acids rapidly and did not result in selective distribution of erucic acid.Geotrichum candidum lipase released C20 and C22 fatty acids extremely slowly, resulting in their accumulation in the di- and triglyceride fractions.
Less than 2% of the total erucic acid was found in the free fatty acid (FFA) fraction. Lipase fromCandida rugosa released erucic acid more slowly than C20 and C18 fatty acids at 35°C but only resulted in a limited accumulation of the
erucic acid in the di- and triglyceride fractions. However, when hydrolysis catalyzed byC. rugosa lipase was carried out below 20°C, the reaction mixture solidified and was composed solely of FFAs and diglycerides. The
diglyceride fraction contained approximately 95% erucic acid while about 20% of the total erucic acid was found in the FFA
fraction. It is concluded that hydrolysis at low temperature withC. rugosa lipase results in a higher purity of erucic acid in the glyceride fraction than can be obtained withG. candidum lipase, but with considerable loss of erucic acid to the FFA fraction. 相似文献
3.
Yuji Shimada Akio Sugihara Yumi Minamigawa Kenichi Higashiyama Kengo Akimoto Shigeaki Fujikawa Sadao Komemushi Yoshio Tominaga 《Journal of the American Oil Chemists' Society》1998,75(9):1213-1217
An attempt was made to enrich arachidonic acid (AA) from Mortierella single-cell oil, which had an AA content of 25%. The first step involved the hydrolysis of the oil with Pseudomonas sp. lipase. A mixture of 2.5 g oil, 2.5 g water, and 4000 units (U) Pseudomonas lipase was incubated at 40°C for 40 h with stirring at 500 rpm. The hydrolysis was 90% complete after 40 h, and the resulting
free fatty acids (FFA) were extracted with n-hexane (AA content, 25%; recovery of AA, 91%). The second step involved the selective esterification of the fatty acids with
lauryl alcohol and Candida rugosa lipase. A mixture of 3.5 g fatty acids/lauryl alcohol (1:1, mol/mol), 1.5 g water, and 1000 U Candida lipase was incubated at 30°C for 16 h with stirring at 500 rpm. Under these conditions, 55% of the fatty acids were esterified,
and the AA content in the FFA fraction was raised to 51% with a 92% yield. The long-chain saturated fatty acids in the FFA
fraction were eliminated as urea adducts. This procedure raised the AA content to 63%. To further elevate the AA content,
the fatty acids were esterified again in the same manner with Candida lipase. The repeated esterification raised the AA content to 75% with a recovery of 71% of its initial content. 相似文献
4.
Yuji Shimada Akio Sugihara Yumi Minamigawa Kenichi Higashiyama Kengo Akimoto Shigeaki Fujikawa Sadao Komemushi Yoshio Tominaga 《Journal of the American Oil Chemists' Society》1998,75(12):1213-1217
An attempt was made to enrich arachidonic acid (AA) from Mortierella single-cell oil, which had an AA content of 25%. The first step involved the hydrolysis of the oil with Pseudomonas sp. lipase. A mixture of 2.5 g oil, 2.5 g water, and 4000 units (U) Pseudomonas lipase was incubated at 40°C for 40 h with stirring at 500 rpm. The hydrolysis was 90% complete after 40 h, and the resulting
free fatty acids (FFA) were extracted with n-hexane (AA content, 25%; recovery of AA, 91%). The second step involved the selective esterification of the fatty acids with
lauryl alcohol and Candida rugosa lipase. A mixture of 3.5 g fatty acids/lauryl alcohol (1:1, mol/mol), 1.5 g water, and 1000 U Candida lipase was incubated at 30°C for 16 h with stirring at 500 rpm. Under these conditions, 55% of the fatty acids were esterified,
and the AA content in the FFA fraction was raised to 51% with a 92% yield. The long-chain saturated fatty acids in the FFA
fraction were eliminated as urea adducts. This procedure raised the AA content to 63%. To further elevate the AA content,
the fatty acids were esterified again in the same manner with Candida lipase. The repeated esterification raised the AA content to 75% with a recovery of 71% of its initial content. 相似文献
5.
Beef tallow and cottonseed oil were mixed with a pure butterfat in the ratios of 2%, 4% and 6% to obtain admixtures of beef
tallow with butterfat and cottonseed oil with butterfat. The hydrolysis of individual triglycerides was carried out using
the lipase to obtain 2-monoglycerides. The results indicated that butterfat had a higher percentage of C14:0 and C16:0 acids
than found in the triglycerides and 2-monoglycerides of beef tallow and cottonseed oil.
Beef tallow contained a higher proportion of C18:0 and C18:1 acids than butterfat and cottonseed oil triglycerides or 2-monoglycerides.
Cottonseed oil had a higher percentage of C18:2 acid located in triglyceride or 2-monoglyceride than found in butterfat or
beef tallow triglycerides and 2-monoglycerides.
The analysis of the samples of butterfat containing 2%, 4% and 6% beef tallow revealed that the addition of beef tallow to
butterfat affected the fatty acid composition of butterfat triglycerides and 2-monoglycerides with C18:0 and C18:1 acids;
the effect was increased with increasing percentages of beef tallow.
The addition of cottonseed oil to butterfat in the ratios of 2%, 4% and 6% affected the fatty acid composition of butterfat
triglycerides and 2-monoglycerides. It was found that both C18:1 and C18:2 increased as the added cottonseed oil percentages
increased. 相似文献
6.
Per Langholz Peter Andersen Torben Forskov Walther Schmidtsdorff 《Journal of the American Oil Chemists' Society》1989,66(8):1120-1123
Lipases catalyze the reesterification or hydrolysis of fats and oils and may show specificity towards certain fatty acids.
An immobilized lipase from the fungusMucor miehei shows specificity towards docosahexaenoic acid (DHA) (DHA being a poor substrate), whether the DHA is used as a free fatty
acid (FFA) as substrate in esterification with methanol, or fatty acid methyl ester (FAME) is used as a substrate for hydrolysis.
The specificity of the lipase fromM. miehei may be applied to concentrate DHA originating from a marine oil in either the FAME or the FFA fraction, which can be separated. 相似文献
7.
Victor M. Balcão Asmo Kemppinen F. Xavier Malcata Paavo J. Kalo 《Journal of the American Oil Chemists' Society》1998,75(10):1347-1358
Butterfat was chemically modified via combined hydrolysis and interesterification, catalyzed by a commercial lipase immobilized onto a bundle of hydrophobic hollow
fibers. The main goal of this research effort was to engineer butterfat with improved nutritional properties by taking advantage
of the sn-1,3 specificity and fatty acid specificity of a lipase in hydrolysis and ester interchange reactions, and concomitantly decrease
its level of long-chain saturated fatty acid residues (viz., lauric, myristic, and palmitic acids) and change its melting properties. All reactions were carried out at 40°C in a solvent-free
system under controlled water activity, and their extent was monitored via chromatographic assays for free fatty acids, esterified fatty acid moieties, and triacylglycerols; the thermal behavior of
the modified butterfat was also assessed via calorimetry. Lipase-modified butterfat possesses a wider melting temperature range than regular butterfat. The total saturated
triacylglycerols decreased by 2.2%, whereas triacylglycerols with 28–46 acyl carbons (which contained two or three lauric,
myristic, or palmitic acid moieties) decreased by 13%. The total monoene triacylglycerols increased by 5.4%, whereas polyene
triacylglycerols decreased by 2.9%. The triacylglycerols of interesterified butterfat had ca. 10.9% less lauric, 10.7% less myristic, and 13.6% less palmitic acid residues than those of the original butterfat. 相似文献
8.
Uptake of blood triglyceride by various tissues 总被引:3,自引:0,他引:3
Triglycerides are transported in the blood in chylomicrons and very low density lipoproteins. Electron microscopic studies
indicate that these particles, which range in diameter from 0.03–0.6 μ, cannot cross the capillary endothelium in most tissues.
There is now considerable evidence that the triglycerides are hydrolyzed to free fatty acids (FFA) during uptake and that
this process is catalyzed by lipoprotein lipase. The enzyme is found in nearly all tissues that utilize circulating triglyceride,
and the level of activity, in individual tissues, varies with nutritional and physiological states that affect triglyceride
uptake, such as fasting, diabetes and pregnancy. Studies in perfused adipose tissue with doubly labeled chylomicrons showed
that hydrolysis occurs outside of the blood stream. Two-thirds of the fatty acids are incorporated into tissue triglyceride
and the rest are release as FFA, with glycerol, to the blood. Infusion of heparin causes immediate release of lipoprotein
lipase activity to the blood and decreases the amount of chylomicron-triglyceride hydrolyzed by the tissue. Electron microscopic
cytochemical studies showed that hydrolysis of blood glycerides by lipoprotein lipase in adipose tissue occurs within the
capillary endothelial cells and in the subendothelial space near the pericytes, but not in the capillary lumen or near the
fat cells. The results indicate that the fatty acids of chylomicrons cross the capillary endothelium as glycerides and FFA,
within a membrane-bounded system, and cross the extravascular space to the fat cells as FFA.
Presented at the AOCS Meeting, Atlantic City, October 1971. 相似文献
9.
Purification of docosahexaenoic acid from tuna oil by a two-step enzymatic method: Hydrolysis and selective esterification 总被引:3,自引:0,他引:3
Yuji Shimada Kazuaki Maruyama Akio Sugihara Shigeru Moriyama Yoshio Tominaga 《Journal of the American Oil Chemists' Society》1997,74(11):1441-1446
Purification of docosahexaenoic acid (DHA) was attempted by a two-step enzymatic method that consisted of hydrolysis of tuna
oil and selective esterification of the resulting free fatty acids (FFA). When more than 60% of tuna oil was hydrolyzed with
Pseudomonas sp. lipase (Lipase-AK), the DHA content in the FFA fraction coincided with its content in the original tuna oil. This lipase
showed stronger activity on the DHA ester than on the eicosapentaenoic acid ester and was suitable for preparation of FFA
rich in DHA. When a mixture of 2.5 g tuna oil, 2.5 g water, and 500 units (U) of Lipase-AK per 1 g of the reaction mixture
was stirred at 40°C for 48 h, 83% of DHA in tuna oil was recovered in the FFA fraction at 79% hydrolysis. These fatty acids
were named tuna-FFA-Ps. Selective esterification was then conducted at 30°C for 20 h by stirring a mixture of 4.0 g of tuna-FFA-Ps/lauryl alcohol (1:2, mol/mol), 1.0 g water, and 1,000 U of Rhizopus delemar lipase. As a result, the DHA content in the unesterified FFA fraction could be raised from 24 to 72 wt% in an 83% yield.
To elevate the DHA content further, the FFA were extracted from the reaction mixture with n-hexane and esterified again under the same conditions. The DHA content was raised to 91 wt% in 88% yield by the repeated
esterification. Because selective esterification of fatty acids with lauryl alcohol proceeded most efficiently in a mixture
that contained 20% water, simultaneous reactions during the esterification were analyzed qualitatively. The fatty acid lauryl
esters (L-FA) generated by the esterification were not hydrolyzed. In addition, L-FA were acidolyzed with linoleic acid, but
not with DHA. These results suggest that lauryl DHA was generated only by esterification. 相似文献
10.
The enantiomeric nature of the triglycerides of bovine milk fat was reinvestigated by determining the stereospecific distribution
of fatty acids in rearranged butterfat, following partial hydrolysis with pancreatic lipase, and in certain molecular distillates
of native butterfat, following Grignard degradation. The results with rearranged butterfat confirmed the validity of pancreatic
lipase hydrolysis as a means of generating representative diglycerides from milk fat triglycerides. The Grignard degradation
and lipolysis gave identical distributions for fatty acids when included as part of the assay system in the stereospecific
analysis. Characteristically, butyric acid and the other short chain acids occupied the 3 position in the native butterfat,
while in the rearranged oil they were distributed more or less randomly. Gas chromatographic analysis of the short chain glycerides
on polyester columns allowed an effective resolution of butyryl, caproyl and caprylyl glycerides of identical numbers of total
acyl carbons and double bonds. The method was especially well suited for resolution of the 2,3-diglycerides, which were recovered
either as the more polar fraction from thin layer chromatography of the X-1,2-diacylglycerols, or by acetolysis of the residual
phenolphosphatides resulting from phospholipase A digestion. It was shown that butyric acid in the 3 position was preferentially
paired with myristic, palmitic and oleic acid in the 2 position, and palmitic and oleic acid in the 1 position, which was
also characteristic of the other short chain acids.
One of eight papers presented in the symposium “Milk Lipids,” AOCS Meeting, Ottawa, September 1972. 相似文献
11.
Yuji Shimada Norihito Sakai Akio Sugihara Hiroyuki Fujita Yo Honda Yoshio Tominaga 《Journal of the American Oil Chemists' Society》1998,75(11):1539-1544
γ-Linolenic acid (GLA) is a physiologically valuable fatty acid, and is desired as a medicine, but a useful method available
for industrial purification has not been established. Thus, large-scale purification was attempted by a combination of enzymatic
reactions and distillation. An oil containing 45% GLA (GLA45 oil) produced by selective hydrolysis of borage oil was used
as a starting material. GLA45 oil was hydrolyzed at 35°C in a mixture containing 33% water and 250 U/g-reaction mixture of
Pseudomonas sp. lipase; 91.5% hydrolysis was attained after 24 h. Film distillation of the dehydrated reaction mixture separated free
fatty acids (FFA; acid value 199) with a recovery of 94.5%. The FFA were selectively esterified at 30°C for 16 h with two
molar equivalents of lauryl alcohol and 50 U/g of Rhizopus delemar lipase in a mixture containing 20% water. The esterification extent was 52%, and the GLA content in the FFA fraction was
raised to 89.5%. FFA and lauryl esters were not separated by film distillation, but the FFA-rich fraction contaminated with
18% lauryl esters was recovered by simple distillation. To further increase the GLA content, the FFA-rich fraction was selectively
esterified again under similar conditions. As a result, the GLA content in the FFA fraction was raised to 97.3% at 15.2% esterification.
After simple distillation of the reaction mixture, lauryl esters contaminating the FFA-rich fraction were completely eliminated
by urea adduct fractionation. When 10 kg of GLA45 oil was used as a starting material, 2.07 kg of FFA with 98.6% GLA was obtained
with a recovery of 49.4% of the initial content. 相似文献
12.
Isolation of tocopherol and sterol concentrate from sunflower oil deodorizer distillate 总被引:10,自引:0,他引:10
The isolation of tocopherols and sterols together as a concentrate from sunflower oil deodorizer distillate was investigated.
The sunflower oil deodorizer distillate was composed of 24.9% unsaponifiable matter with 4.8% tocopherols and 9.7% sterols,
28.8% free fatty acid (FFA) and 46.3% neutral glycerides. The isolation technology included process steps such as biohydrolysis,
bioesterification and fractional distillation. The neutral glycerides of the deodorizer distillates were hydrolyzed byCandida cylindracea lipase. The total fatty acids (initial FFA plus FFA from neutral glycerides) were converted into butyl esters withMucor miehei lipase. The esterified product was then fractionally distilled in a Claisen-vigreux flask. The first fraction, which was
collected at 180–230°C at 1.00 mm of Hg for 45 min, contained mainly butyl esters, hydrocarbons, oxidized products and some
amount of free fatty acids. The fraction collected at 230–260°C at 1.00 mm Hg for 15 min was rich in tocopherols (about 30%)
and sterols (about 36%). The overall recovery of tocopherols and sterols after hydrolysis, esterification and distillation
were around 70% and 42%, respectively, of the original content in sunflower oil deodorizer distillate. 相似文献
13.
Luis Vázquez Leslie Kleiner Casimir C. Akoh 《Journal of the American Oil Chemists' Society》2012,89(9):1655-1662
The concentration of stearidonic acid (SDA, 18:4 n-3) in free fatty acids (FFA) formed by selective esterification with dodecanol (lauryl alcohol) was studied. For this purpose, modified soybean oil (initial SDA content, ~23 %) was converted into its corresponding FFA by chemical hydrolysis. In a second step, the resulting FFA were esterified with dodecanol. Process variables such as the type of biocatalyst (lipase), substrate molar ratio and amount of lipase were evaluated. The best SDA concentration (58 %) and recovery (94 %) were attained by performing the esterification reaction for 4 h, with 1:1 molar ratio (dodecanol:FFA), and 5 % (w/w) Candida rugosa lipase as biocatalyst. It was observed that SDA was concentrated in the unesterified fraction. 相似文献
14.
Betty Mbatia Bo Mattiasson Francis Mulaa Patrick Adlercreutz 《European Journal of Lipid Science and Technology》2011,113(6):717-723
PUFA from oil extracted from Nile perch viscera were enriched by selective enzymatic esterification of the free fatty acids (FFA) or by hydrolysis of ethyl esters of the fatty acids from the oil (FA‐EE). Quantitative analysis was performed using RP‐HPLC coupled to an evaporative light scattering detector (RP‐HPLC‐ELSD). The lipase from Thermomyces lanuginosus discriminated against docosahexaenoic acid (DHA) most, resulting in the highest DHA/DHA‐EE enrichment while lipase from Pseudomonas cepacia discriminated against eicosapentaenoic acid (EPA) most, resulting in the highest EPA/EPA‐EE enrichment. The lipases discriminated between DHA and EPA with a higher selectivity when present as ethyl esters (EE) than when in FFA form. Thus when DHA/EPA were enriched to the same level during esterification and hydrolysis reactions, the DHA‐EE/EPA‐EE recoveries were higher than those of DHA/EPA‐FFA. In reactions catalysed by lipase from T. lanuginosus, at 26 mol% DHA/DHA‐EE, DHA recovery was 76% while that of DHA‐EE was 84%. In reactions catalysed by lipase from P. cepacia, at 11 mol% EPA/EPA‐EE, EPA recovery was 79% while that of EPA‐EE was 92%. Both esterification of FFA and hydrolysis of FA‐EE were more effective for enriching PUFA compared to hydrolysis of the natural oil and are thus attractive process alternatives for the production of products highly enriched in DHA and/or EPA. When there is only one fatty acid residue in each substrate molecule, the full fatty acid selectivity of the lipase can be expressed, which is not the case with triglycerides as substrates. 相似文献
15.
Yuji Shimada Nobuhiro Fukushima Hiroyuki Fujita Yo Honda Akio Sugihara Yoshio Tominaga 《Journal of the American Oil Chemists' Society》1998,75(11):1581-1586
A 46% γ-linolenic acid (GLA)-containing oil was produced by selective hydrolysis of borage oil (GLA content, 22%) at 35°C
for 15 h in a mixture containing 50% water and 20 units (U)/g reaction mixture of Candida rugosa lipase. The GLA content was not raised over 46%, even though the hydrolysis extent was increased by extending the reaction
time and by using a larger amount of the lipase. However, 49% GLA-containing oil was produced by hydrolysis in a reaction
mixture with 90% water. This result suggested that free fatty acids (FFA) that accumulated in the mixture affected the apparent
fatty acid specificity of the lipase in the selective hydrolysis and interfered with the increase of the GLA content. To investigate
the kinetics of the selective hydrolysis in a mixture without FFA, glycerides containing 22, 35, and 46% GLA were hydrolyzed
with Candida lipase. The result showed that the hydrolysis rate decreased with increasing GLA content of glycerides, but that the release
rate of GLA did not change. Thus, it was found that the apparent fatty acid specificity of the lipase in the selective hydrolysis
was also affected by glyceride structure. When 46% GLA-containing oil was hydrolyzed at 35°C for 15 h in a mixture containing
50% water and 20 U/g of the lipase, GLA content in glycerides was raised to 54% at 20% hydrolysis. Furthermore, GLA content
in glycerides was raised to 59% when the hydrolysis extent reached 60% using 200 U/g of the lipase. These results showed that
repeated hydrolysis was effective to produce the higher concentration of GLA oil. Because film distillation was found to be
extremely effective for separating FFA and glycerides, large-scale hydrolysis of borage oil was attempted. As a result, 1.5
kg of 56% GLA-containing oil was obtained from 7 kg borage oil by repeated reaction. 相似文献
16.
Recycled vegetable oil (RVO) is a relatively cheap raw material for biodiesel production, but biodiesel grade methyl ester
yields from RVO were found to be considerably lower than those from pure plant oil. The present paper investigates sources
of yield loss during methanolysis of RVOs with free fatty acids (FFA) contents of 0.4–3.3%, and makes suggestions for the
improvement of methyl ester yields. Data presented here indicated that yield losses of methyl esters during methanolysis were
due to triglyceride and methyl ester hydrolysis and to the dissolution of methyl esters in the glycerol phase. Hydrolysis
of triglycerides and methyl esters seemed to be the only side reaction causing yield losses, and the amount of fatty acids
from hydrolysis increased with concentration of the potassium hydroxide catalyst. Dissolution of methyl esters in the glycerol
phase was probably caused by the detergent effect of potassium salts of fatty acids originating from FFA in the RVO and from
triglyceride hydrolysis, and the amount of dissolved methyl esters increased with FFA content of the RVO. The FFA content
of the RVO had no effect on hydrolysis, and the amount of triglycerides and methyl esters hydrolysed during methanolysis remained
constant with increasing FFA content of the RVO. 相似文献
17.
U. Stall 《European Journal of Lipid Science and Technology》1993,95(6):231-236
The Influence of Fatty Acids in Triglycerides on the Digestion of Dietary Fats by Pancreatic Lipase The digestion of dietary fats by pancreatic lipase was studied in in-vitro-experiments. We tested the following fats: coconut, butterfat, cocoabutter, lard and oil of corn germ. The breakdown of triglycerides was followed up by monitoring the free fatty acids and glycerol. Additionally we analyzed the fatty acid distribution by gas-liquid chromatography of triglycerides, 1,2-diglycerides and 2-monoglycerides. Fatty acids with a chain length from C10C20 were determined by gas chromatography. Short chain fatty acids were not regarded separately. As pancreatic lipase has a positional specificity for the 1- and 3-position of a triglyceride there is information on the distribution of fatty acids in fats and of their digestion by such experiments. For the resorption of the fatty acids it may be of a certain importance in which position it is esterified in the fat when it is hydrolysed in gut. The hydrolysis of fats used in these experiments was quite different. This can be explained by the fatty acid distribution, the chain length and by a varying rate of emulsification of fats in an aqueous phase. 相似文献
18.
P. Villeneuve M. Pina D. Montet J. Graille 《Journal of the American Oil Chemists' Society》1995,72(6):753-755
Short-chain fatty acids are usually located at positionsn-3 in natural triglycerides, particulary in dairy fats. As a result, it is extremely difficult to differentiate betweensn-3 stereospecificity and short-chain typoselectivity in many lipases and acyltransferases that perform in this way. This ambiguity
can be removed through successive use of a chiral triglyceride with a short fatty acid in positionsn-1 and of its racemic in controlled hydrolysis reactions. After checking that the proposed method effectively confirmed the
type of activity of control biocatalysts (Candida cylindracea nonspecific lipase andMucor miehei 1–3 regiospecific lipase), we confirmed thatCarica papaya latex has a strictsn-3 stereospecificity. 相似文献
19.
Lipid metabolism in germinating flaxseed 总被引:1,自引:0,他引:1
Flaxseed was germinated in the dark at 25C for 90 hr and the amt of triglycerides, free fatty acids (FFA) and phospholipids,
as well as the fatty acid composition of each, were determined at 18-hr intervals. The amt of FFA increased greatly during
germination. There was no preferential metabolism of any particular fatty acid in either the triglyceride or FFA fractions.
The percentage of linolenic acid in the phospholipids increased as germination progressed. Behenic, lignoceric and cerotic
acids were observed in the FFA fraction after 54 hr of germination. Odd-numbered saturated and unsaturated acids, indicative
of ana-oxidation mechanism, were observed in the FFA fraction at 54 hr and in the triglyceride fraction at 72 hr.
Cooperative investigations of the Dept. of Agricultural Biochemistry, North Dakota State University. Fargo, and Crops Research
Div., ARS, USDA. Published by permission of the Director, North Dakota Agricultural Experiment Station. Journal No. 54.
Crops Research Div., ARS, USDA.
This paper is taken in part from the dissertation for the degree of Doctor of Philosophy of D.C.Z., 1964. 相似文献
20.
Yukihisa Tanaka Jiro Hirano Tadashi Funada 《Journal of the American Oil Chemists' Society》1994,71(3):331-334
Docosahexaenoic acid (DHA) in the free fatty acid (FFA) derived from enzymically hydrolyzed tuna oil was concentrated by partial
titration and precipitation of other FFA as sodium salts with acetone. A triglyceride containing up to 46.2% DHA was synthesized
from the DHA-rich glyceride mixture and FFA by use of an immobilizedChromobacterium viscosum lipase. 相似文献