(鱼屯)毒鱼类中河(鱼屯)毒素直接竞争抑制性酶联免疫吸附试验测定方法的研究

为检测Tun毒鱼类中的河Tun毒素，建立了用抗河Tun毒素单克隆抗体检测河Tun样品中河Tun毒素的直接竞争抑制性酶联免疫吸队试验（ELISA）方法。结果表明，直接ELISA法较间接ELISA法灵敏，最低检出浓度为0.1ng/mL（每检测孔0.01ng），线性范围为5－500ng/mL，方法的加标回收率为73.0%-118.0%。本法与传统的小鼠生物试验相比，两种方法的测定结果之间在统计学上差异无显著性（配对t检验，P＞1），并具有良好的相关性（r＝0.944）。对来自江苏省东海和长江水域的河Tun样品的毒力进行了测定，结果表明本方法简单、快速、灵敏，完全可以满足实际工作的需要。     

进口葡萄酒中赭曲霉毒素A的含量分析

为了解进口葡萄酒中赭曲霉毒素A的含量和污染状况,本研究建立了无需净化、浓缩,直接进样,采用液相色谱-串联质谱联用法(LC-MS/MS)测定葡萄酒中赭曲霉毒素A的方法。赭曲霉毒素A空白基质标准溶液在1.0～20.0 ng/mL浓度范围内线性良好,方法检测限达到0.05 ng/mL,添加浓度为2.0 ng/mL质控样品的回收率为98.7%～113.2%,精密度<5.7%。应用该方法测定了84个进口葡萄酒样品中赭曲霉毒素A的含量,结果表明,赭曲霉毒素的检出率为100%,含量为0.14～1.10 ng/mL,平均含量为0.32 ng/mL。     

花生致敏蛋白Ara h1双抗体夹心ELISA法的建立

为建立用于花生致敏蛋白Ara h 1快速、灵敏、特异检测的双抗体夹心ELISA法,以Ara h 1鼠源单抗(mAb)为捕获抗体,以Ara h 1兔源多抗(pAb)为检测抗体,通过棋盘法优化抗体工作浓度建立方法,并对方法的灵敏度、特异性、准确度、稳定性等检测特性进行鉴定。结果表明,双抗体夹心ELISA法的mAb和pAb最佳工作浓度分别为1∶10 000稀释和1∶8 000稀释;ELISA标准曲线线性范围为4～256ng/mL,检测限为4.16ng/mL;样品添加回收试验的平均回收率为85.9%～94.5%,且该方法与其他常见致敏蛋白无交叉反应;并能在4℃条件下避光密封保存90d内保持检测结果稳定。说明所建立的双抗体夹心ELISA法灵敏特异、准确稳定,能够用于花生致敏蛋白Ara h 1的快速筛查。     

河鲀食用毒性评价与安全措施研究

为了充分利用河资源 ,开展了河安全利用课题研究。利用高效液相色谱法和竞争抑制性酶联免疫吸附试验 (ELISA)方法对安全利用过程中的河毒素进行检测 ,同时引入危害分析和关键控制点 (HACCP)系统 ,对河食品从原料生产到成品的各环节进行危害分析 ,确定关键控制点。制订了原料接收、活鱼暂养、加工去毒、河制作、以及环境卫生的控制标准和措施。结果表明 ,河的分布具有地域差异性。河体内毒素分布很不均匀 ,主要集中在肝、卵巢、皮肤等部位 ,而且季节性变化明显 ,个体差异显著。运用该研究成果自 1995年至 2 0 0 2年进行人体试食试验以来 ,未发现一例中毒症状和体征 ,各项试验证明 ,经去毒工艺处理的河是安全的 ,经食用后不会引起河毒素中毒。这对于保证河资源的安全利用有着重大的意义。     

应用抗黄曲霉毒素单链抗体检测酱油中黄曲霉毒素B1

利用本实验室制备的抗黄曲霉毒素B1的单链抗体(ScFv),通过棋盘实验确定了抗原抗体的最适工作浓度,在此之上根据间接竞争酶联免疫法(ELISA)绘制标准曲线,检测酱油中AFB1的含量;通过改变样品的盐浓度及pH来确定其对ELISA检测结果的影响。研究结果表明,利用ScFv检测黄曲霉毒素的最小检测值为0.10ng/mL,平均加标回收率在84%～109%之间,本文建立的ELISA方法在pH5～8,盐浓度小于10%时较稳定。本文建立的利用抗AFB1的ScFv检测黄曲霉毒素含量的方法方便快捷,稳定性较好,并且成本较低,适合于食品中黄曲霉毒素的检测。     

酶联免疫检测法测定水产品中残留氯霉素的研究

研究了水产品中残留氯霉素的检测方法。采用酶联免疫检测法(ELISA)测定鱼、虾类水产品中残留氯霉素含量,结果表明:检出限为0.009ng/mL,变异系数为3.0%,样品测定的相对偏差为3.4%～7.1%,回收率为92.5%～102.0%。在0.025～2.00ng/mL测定范围内呈良好的线性关系,相关系数R=0.9993。用ELISA测定水产品中氯霉素残留量,实验结果令人满意。     

脱氧雪腐镰刀菌烯醇(DON)直接竞争ELISA方法的建立

利用前期制备得到的DON酶标抗原(辣根过氧化物酶标记)以及抗DON抗体,建立检测食品中DON含量的直接竞争ELISA方法。该方法的检测范围1～100ng/mL,灵敏度达0.56ng/mL,半数抑制浓度IC50为10ng/mL;与DON类似物T-2毒素的交叉反应率为12%;玉米淀粉样品回收率在80.2%～91.1%之间,平均批间变异<15%,平均批内变异<3%。     

三唑磷残留检测直接竞争ELISA试剂盒的研制及应用

采用包被多克隆抗体直接竞争ELISA法研制一种检测水样、蔬菜和粮食样品中三唑磷残留的酶联免疫吸附测定法(ELISA)试剂盒。将不同浓度的三唑磷添加到水样、蔬菜和粮食样品中,用制备的试剂盒检测三唑磷残留并用气相色谱分析法(GC)进行验证,结果样品的加标回收率在65%～125%之间,变异系数<15%。对试剂盒的灵敏度、特异性、精密度、准确度和有效期进行测定,结果表明:研制的试剂盒最低检测限为4.13ng/mL,线性检测范围3.9～500ng/mL;在4℃或-20℃条件下试剂盒有效期可达6个月。用ELISA试剂盒检测三唑磷残留具有可批量分析样品,快速、简便、灵敏、经济的特点,可广泛应用于环境监测和安全食品的生产。     

化学发光酶免疫分析法检测食品中赭曲霉毒素

目的：建立检测食品中赭曲霉毒素(OA)快速灵敏化学发光酶免疫方法。方法：采用棋盘滴定法确定OABSA抗原的包被质量浓度、包被量以及一抗和二抗的工作稀释度,建立间接竞争抑制曲线,确定线性范围、检出限和回收率。结果：确定的检测工作条件为：OA-BSA 最佳包被质量浓度60ng/mL,抗OA 单克隆抗体的最佳工作稀释度1:400,校正曲线线性范围6～400ng/mL,赭曲霉毒素的检出限为0.02ng/mL,50% 抑制质量浓度145ng/mL,在100～2000ng/g 添加水平的回收率范围为83.6%～105.8%。用所建方法对质控样品进行了分析,检测结果符合率达到100%。结论：所建方法准确、灵敏,适用于食品中OA 的筛选。     

超高效液相色谱-串联质谱法测定葡萄酒中赭曲霉毒素A的方法的优化研究

建立QuEChERS技术结合超高效液相-串联三重四级杆质谱(UHPLC-MS/MS)测定葡萄酒中赭曲霉毒素A(OTA)的方法。优化了前处理及检测方法,简化了复杂的净化步骤,样品经乙腈提取浓缩后直接上样。采用Atlantis?T3色谱柱(2.1×150 mm,3μm)进行分离,以甲酸溶液(含0.5 mmol/L甲酸铵)和乙腈作为流动相进行梯度洗脱,以电喷雾负离子模式进行质谱检测,多反应监测(MRM)模式采集,外标法定量。结果表明,赭曲霉毒素A在1.0 ng/mL到50.0 ng/mL范围内线性良好,相关系数为0.99971,检出限0.029 ng/mL,定量限0.098 ng/mL。平均回收率在94%～95.0%,测定值的相对标准偏差(n=6)在1.84%～3.82%。对20种昌黎产红葡萄酒进行测定,未发现达到定量限的葡萄酒。     

Reliable identification of grapevine rootstock varieties using RAPD PCR on woody samples

Since grapevine ( Vitis spp .) rootstock material is being traded increasingly as disbudded woody material a lack of distinctive morphological features on such material necessitates an alternative and reliable means of identification. Methods described here were developed for rapid and efficient extraction of DNA from woody samples rich in phenolic compounds and polysaccharides, and for subsequent identification of varieties by RAPD PCR. Using these methods, and with the application of only one selected RAPD primer, we were able to differentiate sixteen rootstock varieties, including the seven varieties most commonly used in Germany. Problems commonly encountered with reproducibility of RAPD patterns were avoided by choosing primers with a dinucleotide sequence and a high G/C content that allowed a rather high annealing temperature of 45°C. Methods described here should also be useful for other horticultural crops, especially those with woody tissues rich in phenolic compounds and polysaccharides.     

An internet compendium of analytical methods and spectroscopic information for monomers and additives used in food packaging plastics

An internet website (http://cpf.jrc.it/smt/) has been produced as a means of dissemination of methods of analysis and supporting spectroscopic information on monomers and additives used for food contact materials (principally packaging). The site which is aimed primarily at assisting food control laboratories in the European Union contains analytical information on monomers, starting substances and additives used in the manufacture of plastics materials. A searchable index is provided giving PM and CAS numbers for each of 255 substances. For each substance a data sheet gives regulatory information, chemical structures, physico-chemical information and background information on the use of the substance in particular plastics, and the food packaging applications. For monomers and starting substances (155 compounds) the infra-red and mass spectra are provided, and for additives (100 compounds); additionally proton NMR are available for about 50% of the entries. Where analytical methods have been developed for determining these substances as residual amounts in plastics or as trace amounts in food simulants these methods are also on the website. All information is provided in portable document file (PDF) format which means that high quality copies can be readily printed, using freely available Adobe Acrobat Reader software. The website will in future be maintained and up-dated by the European Commission's Joint Research Centre (JRC) as new substances are authorized for use by the European Commission (DG-ENTR formerly DGIII). Where analytical laboratories (food control or other) require reference substances these can be obtained free-ofcharge from a reference collection housed at the JRC and maintained in conjunction with this website compendium.     

Aroma characterization of various apricot varieties using headspace–solid phase microextraction combined with gas chromatography–mass spectrometry and gas chromatography–olfactometry

The characterization of the aromatic profile of several apricot cultivars with molecular tracers in order to obtain objective data concerning the aromatic quality of this fruit was undertaken using headspace–solid phase microextraction (HS–SPME). Six apricot cultivars were selected according to their organoleptic characteristics: Iranien, Orangered, Goldrich, Hargrand, Rouge du Roussillon and A4025. The aromatic intensity of these varieties measured by HS–SPME–Olfactometry were defined and classified according to the presence and the intensity of grassy, fruity and apricot like notes. In the six varieties, 23 common volatile compounds were identified by HS–SPME–GC–MS. Finally, 10 compounds, ethyl acetate, hexyl acetate, limonene, β-cyclocitral, γ-decalactone, 6-methyl-5-hepten-2-one, linalool, β-ionone, menthone and (E)-hexen-2-al were recognized by HS–SPME–GC–O as responsible of the aromatic notes involved in apricot aroma and considered as molecular tracers of apricot aromatic quality which could be utilized to discriminate apricot varieties.     

Tests of potential functional barriers for laminated multilayer food packages. Part I: Low molecular weight permeants

The advent of the functional barrier concept in food packaging has brought with it a requirement for fast tests of permeation through potential barrier materials. In such tests it would be convenient for both foodstuffs and materials below the functional barrier (sub-barrier materials) to be represented by standard simulants. By means of inverse gas chromatography, liquid paraffin spiked with appropriate permeants was considered as a potential simulant of sub-barrier materials based on polypropylene (PP) or similar polyolefins. Experiments were performed to characterize the kinetics of the permeation of low molecular weight model permeants (octene, toluene and isopropanol) from liquid paraffin, through a surrogate potential functional barrier (25 μm-thick oriented PP) into the food simulants olive oil and 3% (w/v) acetic acid. These permeation results were interpreted in terms of three permeation kinetic models regarding the solubility of a particular model permeant in the post-barrier medium (i.e. the food simulant). The results obtained justify the development and evaluation of liquid sub-barrier simulants that would allow flexible yet rigorous testing of new laminated multilayer packaging materials.     

Analysis of benzo[a]pyrene in spiked fatty foods by second derivative synchronous spectrofluorimetry after microwave-assisted treatment of samples

A simple, rapid and inexpensive method has been developed for the determination of benzo[a     

The development of reference materials for paralytic shellfish poisoning toxins in lyophilized mussel. II: Certification study

This paper describes the second part of a project undertaken to develop certified mussel reference materials for paralytic shellfish poisoning toxins. In the first part two interlaboratory studies were undertaken to investigate the performance of the analytical methodology for several PSP toxins, in particular saxitoxin and decarbamoyl-saxitoxin in lyophilized mussels, and to set criteria for the acceptance of results to be applied during the certification exercise. Fifteen laboratories participated in this certification study and were asked to measure saxitoxin and decarbamoyl-saxitoxin in rehydrated lyophilized mussel material and in a saxitoxin-enriched mussel material. The participants were allowed to use a method of their choice but with an extraction procedure to be strictly followed. The study included extra experiments to verify the detection limits for both saxitoxin and decarbamoyl-saxitoxin. Most participants (13 of 15) were able to meet all the criteria set for the certification study. Results for saxitoxin.2HCl yielded a certified mass fraction of <0.07 mg/kg in the rehydrated lyophilized mussels. Results obtained for decarbamoyl-saxitoxin.2HCl yielded a certified mass fraction of 1.59+/-0.20 mg/kg. The results for saxitoxin.2HCl in enriched blank mussel yielded a certified mass fraction of 0.48 +/- 0.06 mg/kg. These certified reference materials for paralytic shellfish poisoning toxins in lyophilized mussel material are the first available for laboratories to test their method for accuracy and performance.     

Announcing a new international peer-reviewed journal:Food Science and Human Wellness now accepting manuscript submission in English

<正>We are pleased to announce the launch of a new international peer-reviewed journal-Food Science and Human Wellness,ISSN 2213-4530,which is an open access journal,produced and hosted by Elsevier B.V.on behalf of Beijing Academy of Food Sciences.Food Science and Human Wellness is an international peer-reviewed English journal that provides a forum for the dissemination of the     

Chinese CTP Market

According to Printing and Printing Equipment Industries Association of China（PEIAC）＇s statistics to the plate manufucturer in China, in 2013, the actual offset plate production has reached 346 million square meters in China. Among them, the CTP production volume was 245 million square meters, up by 11% than that of last year; the total sales of the CTP plate was 239 million square meters, up by 13%.     

Preparatory Work of Print China 2015 is Going Smoothly

正Held at Guangdong Modern International Exhibition Center,Print China 2015 will cover 7exhibition halls,besides the original Hall No.3,4,5,6,7,the newly built F zone of Hall 3 will be used too.The total area will be140,000 square meters.Hall 3:Offset and large printing equipment,package printing equipment,post press