提高mdr1基因体外转染骨髓造血细胞效率的实验研究

目的：探讨mdrl基因体外转染兔骨髓造血细胞的条件及转染后在细胞中的功能性表达。方法：秋水仙碱（90ng／ml）筛选含人mdrlcDNA的产病毒包装细胞PA317-HaMDR1／A,制备浓缩病毒上清液并转染兔骨髓单个核细胞;分别用PCR法、免疫组织化学法和柔红霉素（daunorubicinDNR）排出试验检测mdr1基...     

Quantitative evaluation of the surface membrane receptors of leukemic cells to blood serum transcobalamin-II

Serum protein transcobalamin II (TC-II) is responsible for transport of cobalamins into mammalian cells. A method of quantitative estimation of plasma membrane receptors of hemopoietic cells to TC-II cobalamin complex is suggested. Analysis of mouse leukemia L1210 cells includes the saturation of radiolabelled ligand-receptor complex with papain. The number of receptors and 57CoCNCbl content in one cell is determined by differentiated radioactivity count of solubilized protein complexes and of cytoplasm.     

The effect of autoplasma of patients with breast cancer on the functional activity of natural killer cells and adhering mononuclear cells in peripheral blood in vitro

Autoplasma of 43 breast cancer patients and 30 donors has been studied for its effect on the activity of natural killer (NK) cells and adherent cells phagocytosis. The cytotoxic activity of NK cells and the phagocytic activity of adherent cells as well as the cytotoxic activity of plasma of breast cancer patients toward K-562 cells are found to increase during tumour progression. Autoplasma of cancer patients is shown to block the cytotoxic activity of NK and even more markedly--the phagocytic activity of adherent cells. As a result, breast cancer patients have nearly normal level of the cytotoxic activity of NK in the presence of autoplasma, whereas the phagocytic activity of adherent mononuclears decreases. Thus, autoplasma of breast cancer patients properly affects the cytotoxic activity of the NK cells and the phagocytic activity of adherent cells.     

Inhibiting effect of a chalone-containing extract of calf pancreatic tissue on the proliferative activity of normal and tumorous pancreatic cells in vitro and in vivo

The pancreatic chalone has been studied for its effect on the proliferative activity and morphology of normal and tumour cells of the pancreas. These findings in experiments in vitro were confirmed by the data of the in vivo experiments. The results show that pancreatic chalone inhibits cell proliferation, decreases mitotic index and causes morphologic changes in cultivated pancreatic cells.     

Spontaneous formation of mixed-cell colonies in the cultivation of hematopoietic cells on soft agar

The spontaneous formation of mixed colonies on soft agar was found on the 7-8th days of the bone marrow cell cultivation. The observed colonies differ from those obtained by Fauser and Messner (1980, 1981) in the independence of any stimulators and faster development in the presence of a stromal underlayer. The stromal underlayer has the typical morphological structure: its cells are compactly packed in the form of a skein with stromal mechanocytes among them.     

Lectin receptors--histochemical markers in the study of leukemias and lymphomas

Data are presented on histochemical determination of lectin receptors on surface membranes of different haematopoietic and lymphoid cells of healthy persons and of patients with leukemias and lymphomas.     

Effect of titanium,copper and iron on silicon solar cells

The effect of Ti, Cu and Fe on silicon solar cells has been investigated. Ti severely degrades cell performance above a concentration of 10¹¹ cm^?3. The presence of 2 × 10¹⁴ cm^?3 Ti results in a 63% loss in cell performance and more than an order of magnitude reduction in carrier lifetime. Ti gives rise to two deep levels in Si at Ev + 0.30 eV and Ec ? 0.27 eV. Copper, at concentrations below 10¹⁶ cm^?3, has negligible effect on cell performance and carrier lifetime. Above 10¹⁶ cm^?3 copper occasionally produces a 10–15% loss in cell performance with a noticeable increase in junction excess current. No recombination centers were found due to Cu, instead considerable precipitation in the starting material was observed. Fe begins to hurt the cell performance above a concentration of 2 × 10¹⁴ cm^?3. Iron at 1.7 × 10¹⁵ cm^?3 results in 46% loss in cell efficiency and about an order of magnitude reduction in lifetime. Fe induces a deep level in silicon at Ev + 0.4 eV. The active center density, for both Ti and Fe, is only a very small fraction of the total impurity content in the starting silicon wafer.     

Effect of cyclophosphane, chlorbutin and degranol on protein biosynthesis in the lymphocytes of donors and chronic lympholeukemia patients in vitro

The alkylating agents in different concentrations are studied for their effect on protein biosynthesis and the relative content of leu-tRNA. The rate of elongation and termination of polypeptide translation in lymphocytes of donors and of patients with chronic lymphoid leukemia in vitro is studied. The therapeutic doses of cyclophosphamide are shown to inhibit the amino acid incorporation into acid insoluble pool of predominantly leukemic lymphocytes, while chlorbutin and degranol caused the greater suppression of the amino acid incorporation into healthy cells. Chlorbutin, cyclophosphamide and degranol in doses above therapeutic caused approximately 95, 48 and 34% inhibition of the protein synthesis, respectively. The mechanism of action on the translation is different. Only chlorbutin acted directly on the translation rate and also increased leu-tRNA content. Degranol decreased the leu-tRNA content. Cyclophosphamide produces no effect on these processes in vitro.     

人参皂苷Rg1协同rhBMP-2对人牙髓干细胞成牙分化作用研究

目的：探讨不同浓度人参皂苷Rg1、重组骨形态发生蛋白2（rhBMP-2）及两者联合应用对体外培养的人牙髓干细胞（DPSCs）成牙分化的影响。方法：酶消化法获得DPSCs,采用碱性磷酸酶（ALP）活性检测和实时荧光定量RT—PCR测定牙本质涎磷蛋白1（DSPP）和牙本质基质蛋白1（DMP1）mRNA的表达以检测不同浓度人参皂苷Rgt（0．1,0．5,2．5,5,10,20μmol／L）及rhBMP-2（25,50,100,200rig／ha）单独及联合应用对DPSCs成牙分化的影响。结果：人参皂苷Rg1（2．5,5和10μmol／L）组的ALP活性明显高于同期对照组（P〈0．001）,5μmol／L组ALP活性明显高于其它浓度组（P〈0．001）;浓度为50,100,200mg/ml的rhBMP-2其ALP活性明显高于同期对照组（P〈0．001）,100ng／ml rhBMP-2组ALP活性最强。联合应用人参皂苷Rg1（5μmol／L）和rhBMP-2（100ng／ml）DSPP和DMP1 mRNA的表达明显高于单独应用组（P〈0．001）。结论：人参皂苷Rg1可促进体外培养的DPSCs成牙本质分化,人参皂苷：Rg1协同rhBMP-2能明显增强DISCs成牙本质分化。     

Quantitative immunoenzyme determination of the lactoferrin and alpha-lactalbumin in the blood serum of cancer patients

An enzyme-linked immunosorbent assay (ELISA) has been used for quantitative determination of lactoferrin (Lf) and alpha-lactalbumin (alpha-La) in human blood serum. The mean concentration of Lf in normal serum was 265 +/- 21 ng/ml and that of alpha-La did not exceed 5 ng/ml. The increased Lf level (above 450 ng/ml) was found in 16 of 30 (53%) patients with gastrointestinal cancer, in 11 of 25 (44%)--with lung cancer, in 13 of 42 (31%)--with breast cancer and more rarely--in other cancer patients. The increased alpha-La level (above 5.0 mg/ml) was found in 5 of 42 patients with breast cancer and very rarely--in other cancer patients. A conclusion is drawn that serological measurements of Lf and alpha-La were of no diagnostic value for breast cancer.     

竹红菌素光敏杀伤细胞及吸收光谱和HPLC色谱研究

本实验对新型光敏剂——竹红菌素加激光辐照对体外培养细胞PTK_2和HeLa的杀伤力进行了研究,同时还对竹红菌素的吸收光谱和高效液相色谱分析(HPLC)层析结果进行了分析。结果表明竹红菌素可能是一种有效的光动力学反应的光敏剂。     

Effect of surface cooling and blood flow on the microwave heating of tissue

The one-dimensional heat transport equation has been solved for a semi-infinite plane of tissue irradiated by microwave radiation, to determine the effects of thermal convection due to blood flow and transfer of heat from the tissue surface into space on the steady-state temperature distribution in the tissue. For microwaves in the 1 to 10-GHz range, the effective heating depth is 1 to 2 cm, and can be much deeper than the penetration depth of the radiation in the tissue. The position of the maximum tissue temperature can be varied by a centimeter or so by cooling the surface. The results suggest that microwave irradiation of simulated biomaterials is not likely to provide accurate estimates of the actual microwave-induced temperature distribution in tissue.     

Antibodies to the human T-cell leukemia virus in the blood serum of the inhabitants of Sakhalin and Iuzhno-Kuril'sk

Two immunological tests were used in testing of 808 blood serum samples from inhabitants of Sakhalin and of the city of Yuzhno-Kurilsk for antibodies against HTLV-1 etiologically associated with human T-cell leukemia. Antibodies to HTLV-1 were detected in 4.2% of Nivkhs, 1.2% of Oroks and 1.5% of Russians. The highest level of virus-carriers reaching 6.0% was detected in Nivkhs from the middle part of Sakhalin. The antibodies were detected more often in persons of old age and in women.     

Modifying effect of the blood serum from patients with soft-tissue sarcomas on interleukin-2 production and activity

Interleukin-2 (IL-2) production by lymphocytes of a peripheral blood (LPB) in patients with soft tissue sarcomas is considerably lower in comparison with that in donors. Autological serum had either suppressive or stimulative effect on LPB IL-2 production in the observed patients. The investigated serum affected LPB of donors in the same way. Serum of patients with soft tissue sarcomas had the same dual effect on the ability of the active drug IL-2 to proliferate human T-lymphoblasts. Moreover serum-inhibitors of production acted as IL-2 activity stimulators, while serum-stimulators of IL-2 production inhibited the mediator effects.