超声提取蛋黄中类胡萝卜素工艺的优化

实验考察了料液比、超声时间、超声功率三个因素对蛋黄中类胡萝卜素提取率的影响,并采用响应面分析法选出最佳工艺条件。实验结果表明最佳条件为:液料比12m L/g,超声提取时间10 min,超声提取功率200 W。最佳条件下类胡萝卜素提取率实测值为0.229 mg/g,与预测值基本一致,这说明响应面分析法可以优化超声法提取蛋黄总类胡萝卜素的工艺条件。     

响应面法优化枸杞多糖的超声辅助提取工艺

枸杞中的多糖非常有价值,值得开发利用。采用超声波法对枸杞多糖进行提取,采用单一变量的方法,选取4个影响枸杞多糖提取率的因素逐一进行研究,得到的实验结果应用Origin作图,之后选取三个影响较大的因素结合响应面法中的Box-Behnken试验设计对多糖提取操作过程进行优化。实验表明,枸杞多糖的最佳提取工艺条件：乙醇百分含量60.44%,超声时间41.38 min,超声温度40.30℃,吸光度值为0.599,枸杞多糖提取率达到了8.40%。应用响应面法对枸杞多糖进行优化,其最佳工艺参数是稳定、可行的。     

响应面法优化超声辅助提取杜仲多糖的工艺研究

采用超声波辅助水提醇沉法提取杜仲粗多糖。以多糖得率为评价指标,考察料液比、提取温度、提取时间和提取功率四个因素水平,采用Box-Behnken法优化提取工艺。结果显示,超声波辅助提取杜仲多糖的最佳工艺条件为料液比1∶31,提取时间为17 min,提取温度为72℃,提取功率为89 W,理论多糖得率为6.19%,在此条件下得到的实际多糖得率为6.28%,相对误差为1.50%。优化的杜仲多糖提取工艺多糖得率高。     

响应面法优化超声辅助提取杜鹃花黄酮工艺的研究

利用响应面法对超声提取杜鹃花黄酮类成分的工艺条件进行优化。在对乙醇体积分数、料液比、提取时间单因子实验的基础上,根据中心组合设计原理,采用三因子三水平的响应面分析法,在分析各个因素的显著性和交互作用后,得出超声辅助提取杜鹃花黄酮的最优工艺条件:乙醇的体积分数为74.08%,料液比为20.58∶1,提取时间为32.48 min,此时黄酮平均提取率为8.758%。     

响应面法优化百合多糖的超声辅助提取工艺

在单因素实验的基础上,以液料比、提取时间、超声功率、提取次数为考察因素,以百合多糖得率为评价指标,采用响应面法优化百合多糖的超声辅助提取工艺。确定最佳提取工艺为:液料比21∶1 (mL∶g)、提取时间4 min、超声功率400 W、提取次数2次,在此条件下,百合多糖得率为12.936%,与预测值(13.150%)相差0.214%。该优化提取工艺合理、可行,可用于百合多糖的提取。     

响应面法优化超声辅助提取荷叶总生物碱

采用超声辅助从荷叶中提取荷叶总生物碱,采用单因素实验考察了提取溶剂、溶剂pH值、料液比(g∶mL)、溶剂体积分数、提取时间和提取温度对荷叶总生物碱提取率的影响,采用响应面法优化了荷叶总生物碱的提取工艺条件。优化的工艺条件为:溶剂pH值2.0、提取温度70℃、料液比1∶32.1(g∶mL)、乙醇体积分数71.1%、提取时间65.31min,在该条件下,总生物碱的提取率达到5.06%。     

响应面法优化超声辅助提取金线莲多糖工艺研究

采用单因素试验结合响应面法优化金线莲多糖超声波辅助提取工艺。考察超声温度、超声时间、料液比和超声功率等因素,以金线莲中多糖提取量作为评价指标,采用响应面设计优化超声波辅助多糖提取工艺。经优化最佳超声波辅助金线莲多糖提取工艺:超声温度70℃,超声时间60 min,料液比为1∶49,超声功率为270 W。在此条件下,验证实验得到结果为377.4 mg/g与响应面法预测结果无显著差异,证明可行。实验优化并验证了金线莲多糖超声波辅助最佳提取工艺条件,优化后的金线莲多糖提取工艺稳定、可行。     

响应面法优化超声辅助提取金线莲总黄酮工艺研究

采用响应面法优化设计化金线莲总黄酮超声波辅助提取工艺。以金线莲中总黄酮提取率为考察指标,根据中心组合(Box-Benhnken)试验设计原理,考察超声温度、超声时间、料液比以及乙醇浓度等影响因素对金线莲总黄酮提取效果的影响。最佳工艺为:提取温度45℃,超声时间58 min,料液比为1∶30,乙醇浓度为80%,预测总黄酮提取量为6.25 mg/g,验证实验实际提取量6.20 mg/g与之相比无显著差异。响应面分析法优化后的金线莲提取总黄酮工艺稳定可行,操作简便,提取效率高,可为金线莲总黄酮进一步的药理活性研究、制剂研制等提供基础。     

响应面法优化无患子皂苷超声提取工艺研究

目的:优化超声提取无患子果皮皂苷的工艺.方法:以无患子果皮皂苷得率为评价指标,通过单因素试验考察乙醇浓度、料液比、提取时间3个因素对得率的影响;依据单因素结果,进行Box-Behnken中心组合实验设计,对无患子果皮皂苷的超声提取工艺进行优化.结果:最佳工艺条件为乙醇浓度69.43％,物料比1:21.56,提取时间29...     

响应面法优化固定超声辅助提取玫瑰花黄酮的工艺

以玫瑰花为原料,固定超声法辅助乙醇提取黄酮,并采用响应面法进行工艺优化.主要考查了乙醇体积分数、料液比、提取温度、提取时间对黄酮提取得率的影响,优选较佳提取工艺参数:乙醇体积分数60％,料液比0.5:13 g/mL,提取温度50℃,提取时间76 min,此条件下黄酮提取得率3.77％.     

响应曲面法优化蜂胶中的活性黄酮提取

探讨了超声波辅助技术提取蜂胶中的活性黄酮类化合物的工艺。实验以芦丁为标样,在单因素实验基础上,采用Box-Behnken实验设计方法,以提取温度、固液比、提取时间以及乙醇浓度为自变量,黄酮提取率为响应值,研究各自变量及其交互作用对黄酮提取率的影响。利用Design expert软件,建立了提取率与超声波处理各因素的二次多项式数学模型,获得了蜂胶中黄酮的最佳提取条件为:提取温度51.32℃,固液比1∶10,提取时间12.88 min,乙醇浓度88.86%,最佳黄酮提取率为52.39%。经过实验验证,黄酮提取率可达51.98%。     

响应面法优化金银花中绿原酸的超声提取工艺

为优化金银花中绿原酸的超声提取工艺,在单因素实验基础上,选择超声提取时间、提取溶剂量、乙醇体积分数为自变量,绿原酸得率作为响应值,采用中心组合设计的方法,研究各自变量及其交互作用对绿原酸提取的影响。采用响应面分析软件,模拟得到二次多项式回归方程的预测模型,并确定超声提取最佳工艺条件为提取时间25.55 min/次,提取溶液量为10.87倍,乙醇体积分数为71.46%,绿原酸的平均得率为2.03%。     

Optimization of ultrasound-assisted extraction of anthocyanins from mulberry, using response surface methodology

Mulberry is one of the most widely used traditional Chinese medicines. Anthocyanins are the main bioactive components of mulberry, and possess important biological activities, such as antimicrobial, anti-inflammatory and antioxidant activities. This study investigated the ultrasound-assisted extraction (UAE) of anthocyanins from mulberry by using response surface methodology (RSM). The extraction conditions associated with anthocyanin yield, including extraction solvent, liquid-to-solid rate, temperature and extraction time, are discussed. The optimal conditions obtained by RSM for UAE from mulberry include 63.8% methanol contains 1% (v/v) trifluoroacetic acid (TFA), 43.2 °C temperature, 23.8 (v/w) liquid-to-solid ratio, and 40 min time for the maximum yield (64.70 ± 0.45 mg/g). The results indicated that the UAE can be an effective method for the extraction of some active components from plant materials.     

Supercritical extraction of evening primrose oil: Experimental optimization via response surface methodology

Supercritical carbon dioxide (CO₂) effective extraction parameters (pressure, temperature, static extraction time, and dynamic extraction time) of oil recovery from evening primrose seeds were optimized via response surface methodology (RSM). The results of this study indicated that the linear terms of static and dynamic time and the quadratics of temperature and pressure, as well as the interactions of temperature and static time, pressure and temperature had a significant effect on the oil recovery. The optimum extraction conditions of 14.2 MPa, 47.3°C, 30 min (static extraction time) and 150 min (dynamic extraction time) were obtained. Applying the optimum conditions, a mean experimental recovery of 92.98% (triplicate experiment) was achieved, which is well compatible with the RSM‐predicted value (93.61%). The fatty acid composition of extracted evening primrose oil using supercritical CO₂ was compared with that obtained by Soxhlet method in which minor difference was observed. © 2011 American Institute of Chemical Engineers AIChE J, 2011     

响应面法优化大豆荚壳异黄酮超声-回流萃取工艺

利用超声萃取和回流萃取方法进行单因素实验,选取提取温度、超声时间和乙醇体积分数为影响因子,应用响应面法(RSM)优化大豆荚壳异黄酮的提取条件。结果表明,大豆荚壳异黄酮优化浸提条件为:提取温度83℃、乙醇体积分数为84%、超声时间30min、大豆荚壳颗粒150μm、液料比25:1、回流萃取时间90min、超声功率108W,大豆荚壳总异黄酮的得率为3.5mg/g。响应面法大豆荚壳总异黄酮的提取条件优化合理可行,为提高大豆荚壳总异黄酮的提取率提供了依据。     

响应面法优化螺旋藻中叶绿素的超声提取工艺

Chlorophylls were extracted by using ultrasonic from Spirulina platensis. Single factor examination and response surface analysis experiments were adopted to investigate the effects of extraction time, extraction solvent, solvent concentration, ratio of liquid to solid and extraction grade. The results showed that the optimal process parameters for this method were: extraction time of 56.5 min, ethanol concentration of 48.3% (vol) of ethanol/acetone solvent, and ratio of liquid to solid of 7. 9 ml·g^-1. The optimized chlorophylls extraction yield was 1.28%. The comparison experimental results indicated that the yield of chlorophylls by ultrasonic extraction was higher than that obtained from conventional solvent extraction.     

响应面法优化微波辅助水蒸气蒸馏法提取油樟精油工艺

为了解决油樟精油传统提取工艺时间长、效率低、耗能高和成本高等问题,本文遵循绿色提取理念,采用微波辅助水蒸气蒸馏法提取油樟精油并优化最佳提取工艺。根据单因素试验并结合响应面法建立二次回归模型进行方差分析和诊断,考察物料粒度、微波提取时间和微波提取功率对油樟精油得率的影响,确定最佳提取工艺并对理论结果进行实验验证。结果表明,在油樟精油得率相似的情况下,与传统的水蒸气蒸馏法（2h）相比,微波辅助水蒸气蒸馏法（10min）具有提取时间更短、效率更高和更节约能源的优势。通过二次多项式回归模型极显著（P<0.01）,失拟项不显著（P>0.05）,决定系数为R²=0.989等分析结合诊断图表明该模型对数据拟合良好。微波辅助水蒸气蒸馏法提取油樟精油最佳提取工艺为：物料粒度10目,微波提取时间11min,微波提取功率630W,此条件下油樟精油得率为4.479%,与实验验证结果（4.442%±0.16%）基本一致,验证了二次回归模型的可靠性。该技术较传统工艺提取时间短、效率高、耗能少、成本低,更加绿色安全且容易实现产业化,有望提高油樟精油在日化、美容、医药等各个领域的应用价值。     

响应面分析法优化乳化石蜡制备工艺

采用响应面分析法(RSM)中CCD数学模型优化工艺参数,考察乳化剂用量、乳化时间、乳化温度、搅拌速度各因素之间相互作用对石蜡乳液稳定性的影响。得到乳化石蜡制备的最佳工艺条件:Tween80质量为复合乳化剂质量的32.7%,乳化剂用量为总乳液质量的7.056%,乳化时间为46 min,搅拌速率为1 240 r/min,在此条件下,实验制备得到平均粒径为0.286μm,分散性为0.224的石蜡乳液。结果表明:该模型综合分数预测值为0.656,与实验验证值0.638的相关系数为0.973 2,可用于实际预测。     

Optimization of fractional crystallization on crude biodiesel purification via response surface methodology

In order to improve separation and purification efficiency of biodiesel, an alternative method has been explored which is fractional crystallization. In this study, glycerol that has a higher melting point was formed as a solid phase while the pure biodiesel was remained in liquid phase during the crystallization process. Response surface methodology (RSM) and central composite design (CCD) were employed to search for the optimum operating conditions. The best responses were 0.117 and 46.734% for effective partition constant (K) and concentration efficiency (Eff), respectively, while the optimum operating conditions were coolant temperature at ?9.5°C and stirring rate at 362 rpm.     

响应曲面法优化超声辅助提取油菜籽皮中的原花青素

以乙醇为提取剂,用超声辅助的方法从油菜籽皮中提取了原花青素。在讨论了提取剂体积分数、提取时间、液料比(每克原料加入提取剂的毫升数,简称液料比,以下同)和超声波功率等单因素实验的基础上,运用Box-Behnken中心组合实验和响应曲面法分析了提取剂体积分数、提取时间和液料比3个因素对原花青素提取率的影响,并优化了提取工艺。结果表明,最佳的工艺条件为:乙醇体积分数62.9%,提取时间20.8 min,液料比25 mL/g。在该条件下,原花青素提取率的预测值为5.055 mg/g,验证实验值为5.02 mg/g,说明响应曲面法优化油菜籽皮中原花青素提取工艺是可行的。