共查询到19条相似文献,搜索用时 109 毫秒
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微波萃取、高效液相色谱法测定番石榴叶中的齐墩果酸和熊果酸 总被引:5,自引:0,他引:5
建立了微波萃取、高效液相色谱测定番石榴叶中齐墩果酸和熊果酸的方法。采用分式析因设计实验考察了微波萃取参数的影响,番石榴叶粒度和萃取时间是影响齐墩果酸和熊果酸的微波萃取产率的最重要因素。优化的微波萃取条件如下:60~80 mesh番石榴叶、10 mL乙醇、80℃和10 min。微波萃取与索氏萃取得到的齐墩果酸和熊果酸产率一致,然而,前者仅需10 min,后者则需要4 h。 相似文献
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目的研究熊果酸(Ursolic acid,UA)对人脐静脉血管内皮细胞(Human umbilical vein endothelial cells,HUVECs)增殖及ERK1、c-Jun、c-Myc、Cyclin D1基因mRNA和蛋白表达的影响,探讨熊果酸抑制血管生长的机制。方法体外培养HU-VECs,用不同浓度熊果酸(31.5、62.5、125、250、500μg/ml)处理不同时间(12、24、48 h),MTT法检测细胞增殖抑制率。用125μg/ml熊果酸和100μmol/L PD98059(ERK抑制剂)处理HUVECs 48 h;不同浓度熊果酸处理24 h;125μg/ml熊果酸处理不同时间,RT-PCR和Western blot分别检测HUVECs中ERK1、c-Jun、c-Myc、Cyclin D1基因mRNA的转录水平和蛋白的表达水平。结果不同浓度的熊果酸对HUVECs的增殖均有明显的抑制作用,且呈剂量和时间依赖性;经熊果酸和PD98059处理的HUVECs中ERK1、c-Jun、c-Myc、Cyclin D1基因mRNA和蛋白的表达水平均明显降低,且呈剂量和时间依赖性。结论熊果酸可通过抑制ERK信号转导通路而抑制血管内皮细胞增殖。 相似文献
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用正交实验法优化采用超临界CO2技术萃取车前草中熊果酸的工艺条件。在单因素实验基础上,采取L9(3^3)探讨萃取压力、萃取温度、CO2流量对萃取车前草熊果酸的得率的影响情况,确定了超临界CO2萃取车前草中熊果酸的适宜条件。超临界萃取最优条件为:萃取压力35MPa,萃取温度为40℃,选择无水乙醇为夹带剂,夹带剂质量分数为20%,萃取时间2h,CO2流量为20L/h,产品的出膏率为3.58%,萃取物中熊果酸的含量为6.93%。该法与传统方法相比,具有操作简便快速,溶剂用量少,有效成分提取率高等优点。 相似文献
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Xia EQ Wang BW Xu XR Zhu L Song Y Li HB 《International journal of molecular sciences》2011,12(8):5319-5329
Oleanolic acid and ursolic acid are the main active components in fruit of Ligustrum lucidum Ait, and possess anticancer, antimutagenic, anti-inflammatory, antioxidative and antiprotozoal activities. In this study, microwave-assisted extraction of oleanolic acid and ursolic acid from Ligustrum lucidum was investigated with HPLC-photodiode array detection. Effects of several experimental parameters, such as type and concentration of extraction solvent, ratio of liquid to material, microwave power, extraction temperature and microwave time, on the extraction efficiencies of oleanolic acid and ursolic acid from Ligustrum lucidum were evaluated. The influence of experimental parameters on the extraction efficiency of ursolic acid was more significant than that of oleanolic acid (p < 0.05). The optimal extraction conditions were 80% ethanol aqueous solution, the ratio of material to liquid was 1:15, and extraction for 30 min at 70 °C under microwave irradiation of 500 W. Under optimal conditions, the yields of oleanolic acid and ursolic acid were 4.4 ± 0.20 mg/g and 5.8 ± 0.15 mg/g, respectively. The results obtained are helpful for the full utilization of Ligustrum lucidum, which also indicated that microwave-assisted extraction is a very useful method for extraction of oleanolic acid and ursolic acid from plant materials. 相似文献
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苦丁茶中熊果酸的提取及含量测定研究 总被引:5,自引:0,他引:5
对苦丁茶的提取方法进行考查,并对各种苦丁茶中的熊果酸进行定性鉴别和含量测定。方法考查结果表明:以乙醚为溶剂,索氏提取3.5h为宜,此法重现性和回收率良好。定性鉴别和含量测定结果表明:冬青科苦丁茶熊果酸含量较高,而木樨科苦丁茶不含熊果酸,且熊果酸的含量随贮存时间的延长而降低。 相似文献
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以棕榈油皂粒为皂基制备熊果酸香皂,按照国家标准方法对香皂中游离苛性碱、水分和挥发物含量进行检测,采用平板计数法和动物实验对该香皂的体外抑菌作用和安全性进行研究。结果表明,熊果酸香皂对白色假丝酵母、肺炎克雷伯氏菌、伤寒沙门氏菌、铜绿假单胞菌、MRSA1和MRSA2均具有明显抑菌作用,在熊果酸质量分数为7.5%时抑菌效果最为显著,动物实验表明香皂对小鼠皮肤和眼睛黏膜无不良反应和毒副作用,长期使用不会对皮肤产生刺激性。 相似文献
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Substantial amount of apple pomace (AP) is produced in apple processing industries and is often discarded as waste which becomes a source of polluting the environment. However, the by-product constitutes a promising bioresource of ursolic acid with various biological activities. Therefore, this particular research study was aimed to develop a novel and facile method to separate ursolic acid from AP. First, AP was extracted with ultrasound assistance using methanol as extraction solvent, 2.86% with 10:1 (mL g?1) of liquid–solid ratio, 300 W of ultrasonic power, 50°C of extraction temperature, and 90 min of ultrasonic time. Meanwhile, the disruption effect of ultrasound on AP was observed by scanning electron microscope. Second, a novel and facile method was applied to separation of ursolic acid, then a perfect colorless needle crystal of ursolic acid was obtained with the yield of about 1.34% of dry AP. 相似文献
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为拓展结晶溶剂的范围和提高结晶分离熊果酸和齐墩果酸的性能,引入离子液体作为结晶溶剂。测定了熊果酸和齐墩果酸在六种离子液体+乙醇溶液中的溶解度数据,根据该数据,选取1-辛基-3-甲基咪唑六氟磷酸+乙醇混合溶液作为溶剂对熊果酸和齐墩果酸进行了结晶分离,并采用单因素实验对结晶工艺进行了初步优化。结果表明:在1-辛基-3-甲基咪唑六氟磷酸质量分数5%,熊果酸和齐墩果酸质量比1.5∶1,结晶温度30℃,结晶时间14 h的条件下,结晶产物中齐墩果酸的质量分数可以达到85%左右。 相似文献
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Production of ursolic acid, the anti-hepatitis effective composition from natural plant, Sambucus chinensis Lindl. was carried out and the scale-up preparation technology was studied. Extraction of the herb Sambucus chinensis Lindl. was extracted in reflux with 7 volume times of refluxing ethanol at 80℃ for 1 h for two times. The extract was purified with a seh-made specific impurity remover "YCXY-1" to yield ursolic acid of high purity. A new "Extraction/Gelation" technology for the production of ursolic acid was developed. The specific impurity remover "YCXY-1" showed high effectiveness in purification. The purity of the mass-produced ursolie acid was up to 99.8%. The chemical structure of the product was confirmed by the physicochemical constants and spectroscopic identification. The production of high-purity ursolic acid was optimized with natural plant as raw material and with only ethanol as extracting solvent. The difficulties such as isolation and impurity removal were addressed effectively. The novel technology is reasonable, convenient, practical, low-cost, high-yield, suitable for mass production. 相似文献
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分光光度法测定苦丁茶中的熊果酸含量 总被引:1,自引:0,他引:1
用5%香草醛-冰醋酸及高氯酸显色,在波长548nm处用分光光度法测定了海南苦丁茶中的熊果酸含量。回归方程为Y=0.04473X 0.08073,相关系数r=0.9983。熊果酸平均回收率为101.2%,RSD为2.01%(n=5)。本法操作简便、可行、重现性好。 相似文献
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建立沙棘果皮中齐墩果酸、熊果酸的含量测定方法。采用高效液相色谱法,Nova-Pack型C18柱,乙腈-甲醇-水-醋酸铵(65∶20∶15∶0.5)为流动相,检测波长为210nm;流速0.6mL/min。熊果酸回归方程为y=74.0x+9.12,r=0.9995,线性范围0.11~10.64μg/mL,齐墩果酸回归方程为y=61.6x+7.40,r=0.9992,线性范围0.12~12.36μg/mL。沙棘果皮中熊果酸和齐墩果酸的平均含量为1.187mg/g和0.352mg/g,平均回收率为100.3%、100.5%(n=3),RSD分别为1.47%、1.50%(n=3)。该方法可同时测定沙棘果皮中齐墩果酸和熊果酸的含量,可用于药材质量控制。 相似文献