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用聚乙烯醇缩丁醛将葡萄糖氧化酶(GOD)和纳米银固定于铂丝电极上并采用萘酚绿B作为电子媒介制得了新型葡萄糖酶生物传感器。实验发现,吸附在纳米银表面上的酶稳定且保持生物活性,电子媒介体与纳米银结合显著提高了该传感器的灵敏度。讨论了溶解性媒介体萘酚绿的浓度、溶液的pH值和温度对该电极电流响应的影响。该传感器在优化的实验条件下,对葡萄糖表现出良好的响应特性,如响应快、重现性和稳定性好,传感器线性范围为1.0×10-3~4.0×10-2mol/L,检出限为3.0×10-4mol/L。抗坏血酸、尿酸等对测定均无干扰。 相似文献
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将纳米CdS∶Cu颗粒加入到葡萄糖酶(GOD)和辣根过氧化物酶(HRP)双酶膜中,与导电聚合物聚邻苯二胺(PoPD)经电化学聚合反应而固定此两酶,制备了电流型纳米CdS∶Cu颗粒双酶膜葡萄糖生物传感器,分析了CdS∶Cu纳米颗粒对传感器电流响应的影响,进行了传感器的性能测定。实验表明,引入CdS∶Cu纳米粒子和PoPD后可显著改进传感器响应性能,线性范围为0.55~9.2 mmol/L,检测下限为0.55 mmol/L,响应时间为20 s。稳定工作215天,传感器活性指标无显著变化,且抗干扰性强。 相似文献
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选用掺杂对甲基苯磺酸聚吡咯膜附载纳米铜制得了葡萄糖传感器。在硫酸铜-硫酸-十六烷基三甲基溴化铵(CTMAB)溶液中于-0.1 V,时间600 s,电沉积一层纳米铜于聚吡咯膜上。根据纳米铜对葡萄糖存在的电催化活性和聚吡咯传递电子作用,使电极产生电流响应。由电极的循环伏安图(CV)对比,说明纳米铜对葡萄糖的测定有很好的增敏效果。该传感器对葡萄糖的线性响应范围为5.0×10-5~4.0×10-3mol.L-1,校正曲线的斜率为0.9952,检出限为1.0×10-5mol.L-1。 相似文献
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研究了使用通过硫醇化学连接在金电极表面上的金纳米颗粒(AuNP)的多层以制备基于丙氨酸脱氢酶(AlaDH)的铵(NH_4~+)离子生物传感器。在没有AuNP的情况下,尽管观察到电流响应较高,NH_4~+离子生物传感器没有表现出任何良好的线性响应范围。这项工作表明,加入AuNPs可以导致更高的NH_4~+离子浓度的检测,而不需要稀释高NH_4~+离子浓度样品,反应速度快。 相似文献
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采用化学还原法在由硝酸银水溶液、油酸、二乙胺构成的反应体系中制备油酸包裹的单分散纳米银颗粒,表面改性的纳米银颗粒在非极性有机溶剂中极易分散制成纳米流体。利用透射电镜(TEM)、粉末衍射(XRD)、红外光谱(IR)和紫外-可见光谱(UV-Vis)等对所得产物进行了表征。油酸分子的羧基端通过O与纳米银核发生一定的相互作用,牢牢地包裹在银核外层,使得其另一亲油端,即较长的碳链朝向外部,形成了一层保护膜,有效阻止纳米银间的团聚。50℃制备的表面改性的纳米银粒径均匀,平均粒径6.8 nm。采用紫外-可见光谱法测定纳米流体的热稳定性,结果表明,表面改性的纳米银分散在正庚烷中形成的纳米流体在120℃稳定时间超过14 h。 相似文献
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采用化学还原法在由硝酸银水溶液、油酸、二乙胺构成的反应体系中制备油酸包裹的单分散纳米银颗粒,表面改性的纳米银颗粒在非极性有机溶剂中极易分散制成纳米流体。利用透射电镜(TEM)、粉末衍射(XRD)、红外光谱(IR)和紫外-可见光谱(UV-Vis)等对所得产物进行了表征。油酸分子的羧基端通过O与纳米银核发生一定的相互作用,牢牢地包裹在银核外层,使得其另一亲油端,即较长的碳链朝向外部,形成了一层保护膜,有效阻止纳米银间的团聚。50℃制备的表面改性的纳米银粒径均匀,平均粒径6.8 nm。采用紫外-可见光谱法测定纳米流体的热稳定性,结果表明,表面改性的纳米银分散在正庚烷中形成的纳米流体在120℃稳定时间超过14 h。 相似文献
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Hee Dong Jang Sun Kyung Kim Hankwon Chang Eun Hee Jo Kee Min Roh Ji-Hyuk Choi 《Aerosol science and technology》2013,47(7):538-546
A sensitive glucose biosensor was developed based on the adsorption of glucose oxidase by a three-dimensional silver-graphene-titanium dioxide (3D Ag-GR-TiO2) composite electrode. Aerosol spray pyrolysis was employed to synthesize the 3D Ag-GR-TiO2 composite using a colloidal mixture of a silver acetate precursor (C2H3AgO2), graphene oxide, and TiO2 nanoparticles. The effects of the operating temperature, gas flowrate, and TiO2 concentration on the particle properties were investigated. The particle morphology of all 3D Ag-GR-TiO2 composites was spherical in shape. The average sizes of composites could be controlled from 0.45 to 0.64 μm with the variation of process variables. Ag nanoparticles less than 10 nm in diameter were deposited on the surfaces of the TiO2 nanoparticles and GR after a reduction process. The characteristics of the glucose biosensor fabricated with the as-prepared 3D Ag-GR-TiO2 composite were assessed through cyclic voltammetry measurements. The biosensor exhibited a high current flow as well as clear redox peaks, resulting in a superior ability of the catalyst in terms of the electrochemical reactions. The highest sensitivity of glucose biosensor was obtained by 3D Ag-GR-TiO2 composite, which was 12.2 μA/mM·cm2, among 3D Ag-GR-TiO2, 3D Ag-GR, and 3D GR-TiO2 composites.Copyright 2015 American Association for Aerosol Research 相似文献
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Hongmei Quan 《Electrochimica acta》2010,55(7):2232-5857
Silver nanoparticle-modified composite electrodes were prepared by electroless forming silver nanoparticles in carbon black dispersed electroless silver plating solution, and then incorporating the silver nanoparticle/carbon black mixture in a polystyrene matrix. The electrooxidation of glucose in 0.1 M NaOH was studied by using cyclic voltammetry. The electrode has surface-confined nanoparicles showing effective catalytic behavior in the studies of glucose oxidation. It showed stepwise electrocatalytic oxidation behavior clearly without suppression by high background redox current caused by inner layer silver of disk-type silver electrodes. Initially the glucose was catalytically oxidized by AgO, and then the reaction glucose oxidation intermediates with Ag2O were followed stepwisely. Based on the cyclic voltammetric results, pulsed amperometric detection parameters for flow injection analysis were optimized for sensitive detection of glucose. 相似文献
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An amperometric glucose biosensor is developed, based on immobilization of glucose oxidase (GOX) in an electrochemically polymerized, non-conducting poly(o-aminophenol) (POAP) film at Prussian blue (PB)-modified platinum (Pt) microelectrode. Effects of polymerization cycle number for POAP and PB, applied potential used in the determination, pH value of the detection solution and electroactive compounds on the amperometric response of the sensor were investigated and discussed. The electroactive property and rough surface of PB film result in the improvement of the detection limit and the increase of the maximum response current and sensitivity. The biosensor based on Pt/PB/POAP/GOX electrode has two times lower detection limit, five times larger maximum current and nine times higher sensitivity than those of the biosensor based on Pt/POAP/GOX electrode. Additionally, the biosensor shows fast response time, large response current, and good anti-interferent ability for l-ascorbic acid, uric acid and acetaminophen. Excellent reproducibility and stability of biosensor are also observed. 相似文献
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Prussian Blue (PB) based glucose biosensor was prepared by immobilizing glucose oxidase (GOD) in layer-by-layer (LBL) films with chitosan (Chi) and multi-walled carbon nanotubes (MWNTs). With the increasing of Chi/MWNTs/GOD layers, the response current to glucose was changed regularly and reached a maximum value when the number of layer was six. At the optimized condition, the biosensor exhibits excellent response performance to glucose with a linear range from 1 to 7 mM and a low detection limit of 0.05 mM. The biosensor also shows a high sensitivity of 8.017 μA mM−1 cm−2, which is attributed to the biocompatible nature of the LBL films. Furthermore, the biosensor shows rapid response, good reproducibility, long-term stability and freedom of interference from other co-existing electroactive species such as ascorbic acid and acetaminophen. 相似文献
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Yan-Li Yao 《Electrochimica acta》2007,53(2):278-284
A mediator glucose biosensor has been constructed by immobilizing glucose oxidase at electropolymerized poly(toluidine blue O) film on carbon nanotube modified glass carbon electrode. The toluidine blue O moieties served as redox mediators for enzymatic glucose oxidation and as polymeric network to maintain the biosensor activity. Great enhancement in current response was observed for the glucose biosensor. The detection potential could be decreased to −0.1 V (versus Ag|AgCl), where common interferences such as ascorbic acid, uric acid and acetamidophenol were not oxidized to cause interferences. The amperometric glucose biosensor offered a sensitivity of 14.5 mA M−1 cm−2 for the linear range of 1-7 mM. 相似文献
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《Carbon》2013
A green and efficient approach for the synthesis of graphene decorated with silver nanoparticles is demonstrated by simultaneously reducing both graphene oxide (GO) sheets and silver ions with glucose as the reducing agent and poly(N-vinyl-2-pyrrolidone) (PVP) as the surface modifier. Different silver-containing materials are obtained by changing the synthesis temperature. The oxygen-containing groups of the substrate influence its decoration with the in situ formed silver nanoparticles. The combination of glucose and a silver–ammonia solution, as well as maintaining a good dispersion of GO by using PVP are crucial for the decoration of graphene with silver nanoparticles. The materials exhibit a distinct surface-enhanced Raman scattering effect. 相似文献
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Ergun Ekinci 《Polymer Bulletin》1999,42(6):693-699
Summary
An amperometric biosensor for glucose was constructed in a one-step procedure by the electropolymerization of o-toluidine in the presence of glucose oxidase on Pt substrates in KCl aqueous electrolyte at a potential of 0.5 V vs Ag /
AgCl. The amperometric responses of the prepared polymeric biosensor to the glucose were measured at a potential of 0.7 V
in PBS solution. Results showed that this polymeric sensor exhibited a fast amperometric response time (4–5 s) and a linear
range up to 6 mM glucose with poor stability. Also, it was seen that biosensor responded successfully to glucose injections
in the presence of some interfering species such as lactose, sucrose and urea.
Received: 27 January 1999/Revised version: 18 May 1999/Accepted: 18 May 1999 相似文献
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The electrocatalytic oxidation of glucose on gold nanocomposite electrodes was investigated with cyclic voltammetry. The gold nanocomposite electrodes were prepared by precipitating gold nanoparticles of different sizes from the corresponding colloidal solutions onto planar substrates, and the electrodes exhibited higher catalytic activity for the oxidation of glucose in alkaline solution with a negative shift in oxidation potential and a larger current as compared with bare gold electrodes. The high catalytic activity of the gold nanocomposite electrodes also resulted in easy oxidation of gluconolactone produced in the reaction. The modification of the gold nanocomposite electrodes with silver underpotential deposition led to a further negative shift in potential but a drop in current for the glucose oxidation. 相似文献