共查询到18条相似文献,搜索用时 953 毫秒
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用于生物标记的半导体量子点研究 总被引:1,自引:0,他引:1
半导体量子点的独特光学性质使之成为理想的荧光探针材料,在生物医学领域具有广阔的应用前景.本文评述了目前量子点合成、表面修饰、结合生物分子的方法,以及半导体量子点在生物标记应用中相对于传统有机染料的优点. 相似文献
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《化学试剂》2018,(12)
相对于传统的Ⅱ/Ⅵ族、Ⅲ/Ⅴ族量子点,硅量子点以其良好的生物相容性和优越的光学性能在生物传感、荧光探针及医学诊断等领域得到广泛应用。以氨基乙基氨基异丁基甲基二甲氧基硅烷为硅前体,抗坏血酸钠和抗坏血酸为还原剂,水热法合成了水溶性黄绿色荧光硅量子点。并利用红外光谱仪、X-射线光电子能谱仪、透射电子显微镜对硅量子点进行结构表征。对反应的影响因素如水浴反应时间、水浴温度、反应物的物质的量比、还原剂比例进行了考察和优化。研究表明,制备的硅量子点对Cy5具有明显的淬灭行为。基于硅量子点对Cy5的淬灭行为,建立了Cy5标记单链DNA用于检测肿瘤标志物miRNA-21的方法。该方法的检出范围为0. 5~40 nmol/L,检出限为0. 15 nmol/L,并具有良好的区分单碱基错配的能力。 相似文献
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[目的]构建叶酸分子靶向复合荧光探针,表征基本理化性质,标记细胞。[方法]以巯基乙酸为稳定剂,采用水相合成法制备CdTe/ZnS核壳型量子点。利用荧光分光光度计和红外光谱仪分别对其光致发光和吸收光谱进行表征。采用聚乙烯亚胺作为连接叶酸与量子点的偶联剂,制备叶酸受体靶向的复合荧光探针,并通过透射电镜对其形貌进行表征。在荧光倒置显微镜下观察已知细胞表面叶酸受体阳性的癌细胞对荧光探针的表达图像。[结果]复合荧光探针的光谱性能良好,颗粒分散均匀,能够标记细胞。[结论]复合荧光探针能够标记大鼠肝癌细胞,为其作为临床血液肿瘤治疗中潜在的靶向标记提供依据。 相似文献
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《粘接》2021,48(10)
针对现有量子点影像探针存在荧光性质不可控、生物毒性强的问题,本研究结合现有的CuInS量子点探针,研究制备出一种掺杂Zn离子可简易合成的ZCIS/ZnS量子点纳米探针,并通过改进其水溶性,得到一种可直接应用于医学肿瘤影像的改进ZCIS/ZnS量子点纳米探针。为提高该量子点纳米探针的靶向性,研究根据EDC偶联的原理,通过将改进ZCIS/ZnS量子点纳米探针与cRGD多肽进行偶联,得到ZCIS/ZnS-cRGD量子点纳米探针,并从结构形态、细胞毒性和离体荧光3个方面重点评估了该量子点纳米探针,并通过实验论证了其有效性和实用性。实验结果证明,本研究制备的ZCIS/ZnS-cRGD量子点纳米探针是一种有效的肿瘤靶向探针,可用于实际肿瘤靶向检测。 相似文献
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按随机引物法以地高辛标记HBV DNA探针,与按缺口翻译法以~(32)P标记的探针进行了比较。这两种探针与乙肝血源疫苗及患者血清标本中HBV DNA进行杂交,结果两种探针均可要出1pg同源序列DNA,而地高辛标记的探针,在0.1Pg水平仍可呈现弱着色反应。该探针在一20℃储存6个月,其敏感性不减弱。这种探针在常规诊断实验室更易推广使用。 相似文献
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将培养至对数期的Ag8.653小鼠骨髓癌细胞经裂解、抽提、RnaseA处理和纯化,获得A260nm/A28nm,为2.013、电泳为一条区带的纯化的小鼠骨髓瘤细胞全DNA;该DNA经不同处理,标记两个探针,即全DNA通过缺口转移法,用‘中标记和全DNA用光敏生物素(军事医学科学院二所提供)标记。在比较灵敏度时,将纯化的未标记的小鼠骨髓癌细胞全DNA分别点在两张硝酸纤维膜上(每张膜上分别含20Pg、10pg和5pg),再用’于和光敏生物素标记的探针分别与这两张膜进行斑点杂交。在用光敏生物素标记的探针检测单抗样品时,随机取3批单克隆抗体(分别经… 相似文献
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硫量子点具有发光强度高、毒性低和光化学性能稳定等优势,广泛应用于细胞成像、光电转换和化学催化等领域。鉴于此,本文系统综述了硫量子点的合成方法,光学性能和应用背景。硫量子点的合成方法可分为“自下而上法”和“自上而下法”,对比发现“自上而下法”合成的硫量子点具有更高的荧光量子产率。分析了硫量子点的光学性质,表明其具有紫外吸收特性、荧光特性、光致发光、电化学发光以及光学稳定性。最后,系统介绍了硫量子点在荧光探针、生物成像以及发光器件等领域的重要应用。基于以上分析,深刻剖析了当下硫量子点在前沿应用中亟待解决的问题,展望了未来硫量子点在生物医学、光电催化等新行业、新领域的发展方向。 相似文献
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Single-walled carbon nanotubes (SWCNTs) dotted with Au nanocrystals (Au-SWCNTs) were fabricated by using a two-phase reduction of hydrogen tetrachloroaurate in the presence of thiol groups anchored to SWCNTs for their potential applications in DNA (deoxyribonucleic acid) delivery. To allow a surface reaction on SWCNTs during the metal nucleation and growth processes, Au nanocrystals were grown using a two-phase system. Raman, XPS and transmission electron microscopy results show that the Au nanocrystals were grafted primarily to the sidewalls of the SWCNTs. DNA probes were immobilized on Au-SWCNTs by the conjugation of DNA functionalized at the 3′ end with a thiol group with Au dots of SWCNTs, followed by hybridizion of complementary oligonucleotides, as verified by fluorescence-based measurements. To investigate whether the target DNA hybridized to DNA probes immobilized on Au-SWCNTs, 618-base-pair fragments of amplified DNA were prepared by polymerase chain reaction using plasmid pET-22b as a template. Atomic force micrograph (AFM) images show that the nanorod-bound DNA is recognizable with excellent specificity, indicating the potential use of such material as a versatile gene delivery carrier in gene-based disease therapy. 相似文献
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Trisita Ghosh Rajkumar Sahoo Suman Kumar Ghosh Pallab Banerji Narayan Ch. Das 《Frontiers of Chemical Science and Engineering》2023,17(5):536
The past decade has witnessed a variety of members of the carbon family along with exposure of carbon dots due to their magnificent properties in sensing, bioimaging, catalytic applications, biomedical fields, and so on. Herein, we report the simple hydrothermal method to fabricate photoluminescent doped carbon quantum dots for the detection of noxious lead(II) ions. Lead(II) ion is very venomous for both the environment and human health for which its detection is demanded area in the research field. The as-prepared carbon dots show excellent photostability, low toxicity and significant photoluminescence properties along with good water solubility. Along with these properties, carbon dots have a quantum yield of approximately 15%. In the practical field of application, these carbon dots have been used as sensing probes for the detection of lead(II) ions with a detection limit of 60 nmol·L–1. The fluorescence intensity of carbon dots was remarkably quenched in the presence of the lead(II) ion selectively among all the tested metal ions. Furthermore, we have studied the Stern–Volmer relationship for lead(II) quenching along with the explanation of the probable quenching mechanism. Ability of the doped carbon dots in heavy metal ions sensing in an environmental sample was demonstrated. 相似文献
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Setsuko Tsuboi Dr. Takashi Jin 《Chembiochem : a European journal of chemical biology》2019,20(4):568-575
Near-infrared (NIR)-emitting fluorescent probes are widely used for molecular imaging at the whole-body level. However, NIR-emitting fluorescent probes emitting over λ=700 nm are not suitable for molecular imaging at the cellular level, because most of the conventional fluorescence microscopes have very low optical sensitivity in the NIR region. Thus, to achieve fluorescence imaging at the cellular and whole-body levels by using single probes, visible and NIR-emitting dual-color fluorescent probes are desirable. For dual-color fluorescence molecular imaging, we synthesized fluorescent, recombinant-protein-conjugated, NIR-emitting quantum dots (QDs), in which the recombinant protein consists of enhanced green fluorescent protein (EGFP) and the immunoglobulin binding domain (B1) of protein G. This dual-color fluorescent QD probe binds the Fc region of immunoglobulin G (IgG) through its B1 domain at the QD surface and acts as a molecular-imaging probe at both the cellular and whole-body levels. In this paper, we present the synthesis of fluorescent, recombinant protein (HisEGFP-GB1)-conjugated, NIR-emitting QDs and their application to the dual-color molecular imaging of breast cancer cells in vitro and in vivo. 相似文献
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Sequence-specific nucleic acids exhibiting selective recognition properties towards low-molecular-weight substrates and macromolecules (aptamers) find growing interest as functional biopolymers for analysis, medical applications such as imaging, drug delivery and even therapeutic agents, nanotechnology, material science and more. The present perspective article introduces a glossary of examples for diverse applications of aptamers mainly originated from our laboratory. These include the introduction of aptamer-functionalized nanomaterials such as graphene oxide, Ag nanoclusters and semiconductor quantum dots as functional hybrid nanomaterials for optical sensing of target analytes. The use of aptamer-functionalized DNA tetrahedra nanostructures for multiplex analysis and aptamer-loaded metal-organic framework nanoparticles acting as sense-and-treat are introduced. Aptamer-functionalized nano and microcarriers are presented as stimuli-responsive hybrid drug carriers for controlled and targeted drug release, including aptamer-functionalized SiO2 nanoparticles, carbon dots, metal-organic frameworks and microcapsules. A further application of aptamers involves the conjugation of aptamers to catalytic units as a means to mimic enzyme functions “nucleoapzymes”. In addition, the formation and dissociation of aptamer-ligand complexes are applied to develop mechanical molecular devices and to switch nanostructures such as origami scaffolds. Finally, the article discusses future challenges in applying aptamers in material science, nanotechnology and catalysis. 相似文献