High-resolution multiband polarization epithelial tissue imaging method by sparse representation and fusion

Multiband polarization epithelial tissue imaging is an effective tool to measure tissue's birefringence and structure for quantitative pathology analysis. To discriminate the pathology accurately, high-resolution multiband polarization images are essential. But it is difficult to acquire high-resolution polarization images because of the limitations of imaging systems. The polarization image calculation process can be regarded as image fusion with fixed rules, and multiband polarization images are intrinsically sparse. In this paper, we propose a novel high-resolution multiband polarization image calculation method by utilizing the sparse representation and image fusion method. The multiband images are first represented in the sparse domain and we further introduce total-variation-regularization terms into the sparse representation framework. Then, polarization parameter images are calculated by simultaneous fusion and reconstruction. Higher quality multiband polarization images can be obtained through additional regularization constraint in the fusion process. Extensive experiments validate that the proposed method achieves much better results than many state-of-the-art algorithms in terms of both peak signal-to-noise-ratio and visual perception.     

Three-dimensional imaging analysis of confocal and conventional polarization microscopes by use of Mie scattering theory

We present a three-dimensional imaging analysis of confocal and conventional polarization microscopes by using the extended Mie scattering theory. In the analysis, we calculate the images of a Mie particle whose diameter is comparable with the wavelength of confocal and conventional microscopes. It was found that, when we observe a Mie particle, polarization confocal microscopy is not affected by the polarization distortion that is due to focusing with high-numerical-aperture lenses and does not produce pseudopeaks in the images in comparison with conventional polarization microscopy. The three-dimensional resolution of the polarization microscope and the verification of the proposed analysis method are also discussed.     

Terahertz polarization real-time imaging based on balanced electro-optic detection

A terahertz (THz) polarization real-time imaging system that can effectively reduce experimental time consumption for acquiring a sample's polarization information is achieved. An alternative THz polarization measurement method is proposed. In this method, a <110> zinc-blende crystal is used as the sensor, and the probe polarization is adjusted to detect THz electric fields on the two orthogonal polarization components. The relative sensitivity of the imaging system to the THz polarization angle is estimated to be less than 0.5°. To illustrate the ability of the system, two samples are designed and measured by using the system. From their THz polarization real-time images, each region of these samples can be precisely presented. Experimental results clearly show the special influences of different materials on the THz polarization. This work effectively extends the information content obtained by THz real-time imaging and improves the feasibility of the imaging technique.     

Polarization contrast imaging of biological tissues by polarization-sensitive Fourier-domain optical coherence tomography

Jones matrix imaging of biological samples by a polarization-sensitive Fourier-domain optical coherence tomography has been demonstrated using a two-dimensional CCD camera to obtain two spectra corresponding to the orthogonal polarization components simultaneously. The measurement results of a quarter-wave plate are compared between the two incident polarization sets, H-V linear and R-L circular polarization. Jones matrix imaging of the bovine tendon is demonstrated. Measured Jones matrix images are converted to equivalent Müller matrix images. Local polarization properties are obtained by longitudinal differentiation of Jones matrix components. The layered structure of the bovine tendon and birefringence are revealed.     

Polarimetric imaging method for target enhancement in haze based on polarimetric retrieval

Polarimetric imaging has been proven to be an effective way in detecting the targets of interest in complicated surroundings by analyzing the polarization property, instead of the intensity, of the light emanating from the objects. Unfortunately, polarimetric imaging encounters difficulty when the surroundings are very scattered, where on the one hand the polarization property of the object light usually becomes very faint after a strong depolarized scattering process; on the other hand, the object light will be blended with the atmospheric light scattered by haze particles (airlight). In this paper, we propose a polarimetric imaging retrieval method that can be used for such challenging conditions. Firstly, the airlight radiance is estimated precisely. Then, the airlight is removed from the hazy images. Finally, the residual polarization property of the object light is regained, which ensures the validity of the polarimetric imaging method in these conditions. The experiments confirm that the proposed method is useful in enhancing polarimetric imaging detection in haze.     

Estimation of the degree of polarization in active coherent imagery by using the natural representation

We address the problem of degree of polarization estimation in polarization diversity images. We consider active imaging techniques with laser illumination, which have the appealing feature of revealing contrasts that do not appear in conventional intensity images. These techniques provide two images of the same scene that are perturbed with speckle noise. Because of the presence of nonhomogeneity in the reflected intensity, it can be preferable to perform image analysis of the orthogonal-state contrast image, which is a measure of the degree of polarization of the reflected light when the coherency matrix is diagonal. It has been shown that a simple nonlinear transformation of this orthogonal-state contrast image leads to an image perturbed with additive symmetrical noise on which simple and efficient estimation and detection techniques can be applied. We propose to precisely analyze estimation properties of the degree of polarization using this natural representation. In particular, we determine the Cramer-Rao bound of the polarization degree estimation and the variance of the proposed estimator, and we study the estimator's efficiency as a function of the speckle order for different measurement strategies.     

Target detection in optically scattering media by polarization-difference imaging

Polarization-difference imaging (PDI) was recently presented by us as a method of imaging through scattering media [Opt. Lett. 20, 608 (1995)]. Here, PDI is compared with conventional, polarizationblind imaging systems under a variety of conditions not previously studied. Through visual and numerical comparison of polarization-difference and polarization-sum images of metallic targets suspended in scattering media, target features initially visible in both types of images are shown to disappear in polarization-sum images as the scatterer concentration is increased, whereas these features remain visible in polarization-difference images. Target features producing an observed degree of linear polarization of less than 1% are visible in polarization-difference images. The ability of PDI to suppress partially polarized background variations selectively is demonstrated, and discrimination of target features on the basis of polarization information is discussed. Our results show that, when compared with conventional imaging, PDI yields a factor of 2-3 increase in the distance at which certain target features can be detected.     

太赫兹超表面计算全息

全息术是一种三维成像技术,它已经被应用于多种实际场景。随着计算机科学与技术的迅猛发展,计算全息由于其方便和灵活的特性,已经成为一种广泛应用的全息成像方法。本文回顾了我们近期基于超表面的太赫兹计算全息研究进展。其中,作为全息板的超表面展示出了超越传统光学器件的独特性能。首先,利用超表面实现了对于全息板每个像素的相位振幅同时且独立的调控,进而实现了高质量全息成像。这种新的电磁波操控能力也带来了新的全息成像效果,如利用介质超表面实现了全息像沿传播方向上的连续变化。其次,对超表面在不同偏振态下的响应进行设计,分别实现了线偏振态与频率复用、圆偏振态复用、以及基于表面波的偏振复用超表面全息术。此外,本文提出了依赖于温度变化而主动可控的超表面全息术,为今后计算全息术的设计与实现提供了新的方案,也推动了超表面在实际应用方面的发展。     

Imaging objects hidden in scattering media with fluorescence polarization preservation of contrast agents

A method for fluorescence polarization difference imaging is demonstrated for enhancing the image quality of a luminous object embedded in a random medium. The polarization preservation of light propagating in the scattering medium leads to partially polarized light emission by a contrast-agent dye located inside the object. Subtraction of the images of the luminous object detected at two orthogonal polarization directions improves the image resolution compared with a conventional optical imaging approach.     

General state contrast imaging: an optimized polarimetric imaging modality insensitive to spatial intensity fluctuations

In active polarization imaging, one frequently needs to be insensitive to noninformative spatial intensity fluctuations. We investigate a way of solving this issue with general state contrast (GSC) imaging. It consists in acquiring two scalar polarimetric images with optimized illumination and analysis polarization states, then forming a ratio. We propose a method for maximizing the discrimination ability between a target and a background in GSC images by determining the optimal illumination and analysis states. A further advantage of this approach is to provide an objective way of quantifying the performance improvement obtained by increasing the number of degrees of freedom of a GSC imager. The efficiency of this approach is demonstrated on simulated and real-world images.     

偏振成像在目标探测中的应用

针对偏振度编码成像在目标探测识别中的优点,本文分析了偏振探测的消偏振机理,以及表面散射和体散射对目标偏振度的影响.利用808 nm半导体激光器照明,目标的后向散射光由卡塞格林望远镜接收,经过偏振分光棱镜成像在两个CCD上,通过一次测量获得偏振状态相互垂直的两幅图像,然后计算出目标的强度和偏振度,分别利用强度和偏振度编码成像.实验结果表明,在目标的反射率相近时,强度图像不能区分不同材料的目标,偏振度图像可以区分,融合强度和偏振度图像进行HSI伪彩色编码,可以达到目标探测、分类等目的.     

Polarization-degree imaging contrast in turbid media: a quantitative study

Scattering in biological tissue can degrade imaging contrast and reduce the probe depth. Polarization-based measurement has shown its advantages in overcoming such drawbacks. Here, linear and circular polarization degree imaging is applied to a comblike metal target submerged in Intralipid solutions. Contrasts of the metal bars are measured quantitatively as functions of the Intralipid concentration and the submersion depths. Different behaviors in contrast for linear and circular polarizations are compared. Contributions to the background of circular polarization degree images by backscattering, snake, and diffusive photons are examined carefully.     

Orthogonal polarization simultaneous readout for volume holograms with hybrid angle and polarization multiplexing in LiNbO(3)

The orthogonal polarization simultaneous readout technique in a hybrid-multiplexed memory using angular multiplexing and polarization multiplexing is presented. Twenty holograms were hybrid multiplexed in a single LiNbO(3) crystal with ten angular positions for angular multiplexing. In each angular position of the holographic memory, two images with orthogonal polarization are multiplexed in the same spatial location inside the LiNbO(3) via polarization multiplexing. These two orthogonally polarized images can be reconstructed simultaneously with a linear polarization reading beam, but they can be separated with a polarization beam splitter, and accordingly each can be viewed independently. The exposure schedule for holographic storage using the proposed hybrid-multiplexing technique is derived.     

Optical imaging based on time-resolved Stokes vectors in filamentous tissues

Time-resolved Stokes vector components of light transmitted through filamentous tissues were measured with a view to improving the imaging quality of optical images in such tissues. Temporal profiles of the Stokes vectors and the time-resolved degree of polarization (DOP) were calibrated to produce higher image quality than that of images based on time gating, polarization discrimination, or both. A thin chicken bone inserted into chicken breast tissue with filament orientation in different directions with respect to the direction of input linear polarization was scanned to demonstrate images of higher spatial resolution and contrast based on the measurement of time-resolved DOP.     

Multispectral polarization imaging for observing blood oxygen saturation in skin tissue

We propose a new technique that combines two-dimensional (2D) multispectral imaging and polarization gating for observing the blood oxygen saturation (SpO2) level in human skin tissue. The spectral decomposition of the skin tissue image provides the principal information on blood oxygenation. The polarization gating selects the measurement depth according to the relative orientation of the two polarizers that are placed on a camera and a light source. The combination of these two methods yields multispectral images of the superficial and deep layers of the skin tissue separately. In order to evaluate the blood oxygen, we focus on the multispectral images of the deep site. The SpO2 levels at each image pixel are calculated by means of the partial least squares regression with respect to each reflectance spectrum. The reassignment of the predicted responses retrieves an image whose pixel values represent the relative SpO2 levels. A demonstration experiment for acquiring the multispectral polarization images is performed in the spectral range of 500 to 680 nm, and the SpO2 distributions are obtained.     

Polarization and retinal image quality estimates in the human eye

We have previously studied how polarization affects the double-pass estimates of the retinal image quality by using an imaging polarimeter [Opt. Lett. 24, 64 (1999)]. A series of 16 images for independent combinations of polarization states in the polarimeter were recorded to obtain the spatially resolved Mueller matrices of the eye. From these matrices, double-pass images of a point source for light with different combinations of incoming (first-pass) and outcoming (second-pass) polarization states were reconstructed and their corresponding modulation transfer functions were calculated. We found that the retinal image or, alternatively, the ocular aberrations, are nearly independent of the state of polarization of the incident light (in the first pass). This means that a significant improvement in the ocular optics by using a specific type of polarized light could not be achieved. However, quite different estimates of the retinal image quality are obtained for combinations of polarization states in both the first and the second passes in the double-pass apparatus.     

High-resolution imaging of gigahertz polarization response arising from the interference of reflected surface acoustic waves

The surface polarization caused by traveling SAWs at 1.585 GHz has been imaged using a dynamic homodyne electrostatic force microscope technique. Instead of measuring topographic changes caused by the SAW, the reported technique measures polarization in the piezoelectric substrate arising from mechanical stress caused by the SAW. The polarization associated with this stress field modulates the scanning probe cantilever deflection amplitude, which is extracted using a lock-in-based technique. High-resolution imaging is presented with images of the interference arising from a metal reflector on a SAW device. A mathematical model combining SAW generation and force interactions between the probe and the substrate was used to verify the experimental data. In addition to overcoming the challenge associated with detecting and imaging polarization effects at gigahertz frequencies, this imaging technique will greatly assist the development of SAW-based devices that exploit the reflection and interference of SAWs in areas as diverse as microfluidic mixing, cell sorting, and quantum entanglement.     

Microwave diversity imaging system ‐ principle,methodology and experimental results

Abstract

The basic principle, methodology and experimental results of the automated microwave diversity imaging system developed in the Electromagnetic Research Laboratory of National Taiwan University are presented. The microwave image formulation is based on Bojarski's identity. In practice, accessing the object backscattering information using the frequency, angular and polarization diversity techniques is conducted by the developed microwave measurement system with major parts including dual polarization antennas, network analyzer and a MicroVax III. It is shown that images of a scale model of the space shuttle reconstructed from the measured data can be identified visually. The implications for high‐resolution microwave imaging radar networks are also discussed.     

Measurements of the corneal birefringence with a liquid-crystal imaging polariscope

An imaging polariscope has been used to analyze the spatially resolved polarization properties of living human corneas. The apparatus is a modified double-pass setup, incorporating a liquid-crystal modulator in the analyzer pathway. Keeping the incident polarization state fixed (first passage), we recorded a series of three images of the pupil's plane corresponding to independent polarization states of the analyzer unit. Azimuth and retardation at each point of the cornea were calculated from those images. Results show that the magnitude of retardation increases along the radius toward the periphery of the cornea. Left-right eye symmetry in retardation was also found. Maps of azimuth indicate that the direction of the corneal slow axis is nasally downward.     

Adaptive algorithms for two-channel polarization sensing under various polarization statistics with nonuniform distributions

The polarization of light carries much useful information about the environment. Biological studies have shown that some animal species use polarization information for navigation and other purposes. It has been previously shown that a bioinspired polarization-difference imaging (PDI) technique can facilitate detection and feature extraction of targets in scattering media. It has also been established [J. Opt. Soc. Am. A 15, 359 (1998)] that polarization sum and polarization difference are the optimum pair of linear combinations of images taken through two orthogonally oriented linear polarizers of a scene having a uniform distribution of polarization directions. However, in many real environments the scene has a nonuniform distribution of polarization directions. Using principal component analysis of the polarization statistics of the scene, we develop a method to determine the two optimum information channels with unequal weighting coefficients that can be formed as linear combinations of the images of a scene taken through a pair of linear polarizers not constrained to the horizontal and vertical directions of the scene. We determine the optimal orientations of linear polarization filters that enhance separation of a target from the background, where the target is defined as an area with distinct polarization characteristics as compared to the background. Experimental results confirm that in most situations adaptive PDI outperforms conventional PDI with fixed channels.