Changes in biogenic amines of silver carp (Hypophthalmichthys molitrix) fillets stored at different temperatures and their relation to total volatile base nitrogen,microbiological and sensory score

BACKGROUND: Biogenic amines have received considerable attention owing to their undesirable effects in humans. There are few studies of changes in biogenic amine contents related to freshwater fish. Silver carp is an important freshwater fish species in China. This study aimed to investigate the changes in biogenic amines and their relation to total volatile base nitrogen (TVB‐N), microbiological and sensory score of silver carp fillets stored at 0, 3 and 15 °C. RESULTS: The total biogenic amine contents of all silver carp fillets (regardless of storage time and temperature) ranged from 13.05 to 318.10 mg kg^?1. Putrescine and histamine were the main biogenic amines in silver carp fillets during storage. Cadaverine was only detected after 12 days at 3 °C and after 2 days at 15 °C. Spermidine and spermine contents increased during the early storage period and then slightly decreased. CONCLUSION: Low temperature could control the quality of silver carp fillets by inhibiting the contents of biogenic amines. Putrescine showed significant correlation with TVB‐N, total aerobic content, sensory score, tryptamine and phenylethylamine. Putrescine was a good quality marker of silver carp fillets in the cold chain. Copyright © 2012 Society of Chemical Industry     

Quality changes of European catfish (Silurus glanis) from warm-water aquaculture during storage on ice

Gutted European catfish (Silurus glanis) from warm-water aquaculture was stored in ice for 30 days. The limit of saleability was reached after approximately 20 days, and cooked fillets became inedible after about 27 days. Quality changes were investigated using established analytical methods; pH, total volatile bases (TVB), thiobarbituric acid number and texture measurements by Instron were not usefully predictive of shelf-life or possible storage time. The Fish Tester and Torrymeter values decreased from 848 to 376 and 111 to 61, respectively. On day 27 the total aerobic bacterial counts had reached some 10⁸/cm² skin surface but only 10⁵/g muscle. Hypoxanthine concentrations increased steadily to 17.2 mg/100 g, and ammonia levels increased from 11.5 to 18.7 mg/100 g muscle. There were no differences in the patterns obtained by isoelectric focusing of sarcoplasmic proteins from fresh and spoiled European catfish.     

Biochemical changes in cut vs. intact lamb's lettuce (Valerianella olitoria) leaves during storage

Consumers are oriented towards fresh-cut vegetables that provide phytonutrients useful for preventing stress-related diseases. The aim of this work was to evaluate the cut operations on the quality changes of lamb's lettuce ( Valerianella olitoria L.) cv. Trofy during storage at 4 °C for 8 days. Results showed that chlorophyll and carotenoids reduction was observed after 8 days of storage. In both treatments, total carotenoids after 8 days decreased from 20 to 16 mg g⁻¹ FW. Free and total phenols increased with storage in both treatments. Total phenols were 23% higher in control (32 μmol g⁻¹ FW) compared to cut leaves (25 μmol g⁻¹ FW) after 8 days of storage. Anthocyanins increased after 8 days and reached 30 mg 100 g⁻¹ FW without significant difference between treatments. Ascorbic acid (AsA) and dehydroascorbic (DHA) acid increased in cut leaves compared to control. After 1 day AsA concentration was 3 300 nmol g⁻¹ FW in cut leaves, while in control leaves was 1 500 nmol g⁻¹ FW. Analogously AsA + DHA was higher in cut leaves, 4 100 nmol g⁻¹ FW, while in control leaves the mean was 3 000 nmol g⁻¹ FW. After 5 days of storage the values of AsA returned to initial values, while AsA + DHA were lower.     

Development of Biogenic Amines in Yellowfin Tuna (Thunnus albacares): Effect of Storage and Correlation with Decarboxylase-Positive Bacterial Flora

ABSTRACT: The effects of storage at 0,4,10, and 22°C for 0,1,3,5, and 9 d on the quality of yellowfin tuna fillets as determined by microbiological assessment, development of some biogenic amines, and sensory analysis were studied. Tuna fillets stored at 22 °C for 3 d, 10 °C for 5 d, and 4 °C for 9 d were rated unacceptable for consumption. Those stored at 22 °C for 3 d had total aerobic bacterial count of > 8 log₁₀ CFU/g, a histamine-producing bacterial population of 7 log₁₀ CFU/g, and 832 ppm of histamine, 35.8 ppm of putrescine, and 147 ppm of cadaverine. A comparison of the capillary electrophoresis, AOAC fluorometric method, and gas chromatography showed a very good correlation (r² > 0.99) among these 3 methods for histamine quantitation in tuna samples. Morganella morganii, Enterobacter agglomerans, Enterobacter intermedium, Pseudomonas fluorescens, Proteins vulgaris , and Serratia liquefaciens were the decarboxylase-positive bacterial species isolated by using the Niven's medium and identified during storage, which were responsible for histamine production in test tuna fillets.     

Selected polycyclic aromatic hydrocarbons in smoked tuna, swordfish and Atlantic salmon fillets

The presence of polycyclic aromatic hydrocarbons (PAHs) in commercial smoked fillets of tuna, swordfish and Atlantic salmon was studied. In Europe, the smoking technique is widely used in fish processing and these species are the most representative of the taste of consumers for smoked products. Samples were purchased on the Italian market and analysed by HPLC. Mean concentrations (ng g⁻¹) of acenaphthene (4.4, 6.2, 11.2), phenanthrene (11.5, 18.5, 8.9), anthracene (2.4, 5.2, 1.8), fluoranthene (17.0, 9.4, 4.7), benzo( k )fluoranthene (0.3, 0.1, 0.2) and benzo( a )pyrene (1.3, 0.1, 0.4) were found in Atlantic salmon, tuna and swordfish respectively. Benzo( b )fluoranthene (1.2 ng g⁻¹) was detected only in Atlantic salmon whereas dibenz( a,h )anthracene was never detected in this species, but only in tuna (0.5 ng g⁻¹) and swordfish (1.1 ng g⁻¹). Atlantic salmon contained the highest level of benzo( a )pyrene (2.8 ng g⁻¹) which is below the European regulatory level of 5 ng g⁻¹.     

Dynamic changes of the content of biogenic amines in Chinese rice wine during the brewing process

This study examined the biogenic amine content of the brewing raw material (wheat Qu, seed starter, mash or liquid) during fermentation and during the maturation and storage process. Pre‐column derivatization and high‐performance liquid chromatography were employed to analyse the dynamic changes in biogenic amines during the Chinese rice wine brewing process. Some aliphatic amines (putrescine, cadaverine, spermine) were already found to be present in the glutinous rice and wheat Qu, but the content was below 2.88 mg/kg. None of the amines were detected in the brewing water. The biogenic amine content in the seed starter exhibited a large variation range (16.43–87.72 mg/L), which could influence the total content of biogenic amines prior to fermentation. A much more obvious increase in biogenic amines stemmed from the fermentation process, with the presence of a biogenic amine‐producing bacteria and precursor amino amines. In the first 2 days, the biogenic amine content increased sharply, followed by a slow increase over the next 4 days, and the content reached a maximum value on day 6; subsequently there was a decrease or fluctuation in the concentration. After clarification and cooking, the biogenic amine content increased significantly; however, a slight decrease was observed during storage, especially for putrescine and histamine. Putrescine and tyramine were the most prevalent amines in all of the samples. Overall, the content of biogenic amines was found to be within the safe level for human health, ranging from 54.52 to 146. 35 mg/L. Copyright © 2013 The Institute of Brewing & Distilling     

The effects of different incubation temperatures on the acetaldehyde content and viable bacteria counts of bio-yogurt made from ewe's milk

Yogurt and bio-yogurt were manufactured from ewe's milk using a starter culture and a probiotic culture. Incubation was carried out at 37°C and 42°C until pH 4.6 was reached and the yogurts were stored at 4 +1°C for 14 days. Analysis after 1, 7 and 14 days showed that incubation temperature and storage time significantly influenced overall properties of the samples. During the storage, whey separation and pH decreased, but titratable acidity, lactic acid and volatile fatty acid contents increased. Viable bacterial counts in all bio-yogurts were above 10⁷ cfu g⁻¹ at the end of storage.     

Biogenic amines formation in Atlantic bonito (<Emphasis Type="Italic">Sarda sarda</Emphasis>) fillets packaged with modified atmosphere and vacuum,wrapped in chitosan and cling film at 4 °C

A study was carried out to determine the effect of vacuum packed (VP) and modified atmosphere (MAP) (100% CO₂) packing, chitosan film and cling film (CF) wrapping on the formation of biogenic amines and trimethylamine (TMA) during storage of Atlantic bonito (Sarda sarda) fillets at 4 °C. The biogenic amine contents generally increased in all treatments with increasing storage period. Significant differences were found (p < 0.01) in the levels of cadaverine, tyramine and histamine among the four treatments but it was not detected significant difference for putrescine, phenylethylamine and tryptamine in this level. Spermidine and spermine did not detect throughout the storage period for all experimental conditions. The amine contents of Atlantic bonito were highest in stored in cling film, followed by VP, MAP and chitosan film.     

Use of volatile and non‐volatile amines to evaluate the freshness of anchovies stored in ice

Amounts of volatile and biogenic amines, pH and microbial counts were monitored in Mediterranean anchovy (Engraulis encrasicholus) over 23 days of ice storage with the aim of examining their suitability as indicators of freshness. Counts of Enterobacteriaceae reached the maximum limits stipulated by Spanish regulations after 5 days of storage in ice, while those of mesophilic bacteria took 7 days. Levels of volatile amines hardly increased during ice storage. Amounts of biogenic amines did increase, though there were differences from one amine to the other. Cadaverine, the most abundant biogenic amine, showing the earliest increase, coinciding with the shelf‐life time as determined through Enterobacteriaceae counts. Amounts of histamine remained below the maximum levels established by both European Union and Food and Drug Administration regulations. Other biogenic amines were found at much higher levels than histamine. Results demonstrated the suitability of the biogenic amine index (as the sum of histamine + tyramine + putrescine + cadaverine) as a freshness indicator for anchovies stored in ice, with a limit of acceptability set at 15 mg kg^?1. Copyright © 2005 Society of Chemical Industry     

利用高效液相色谱法测定不同发酵时长郫县豆瓣酱中的8种生物胺

本研究基于高效液相色谱(HPLC)技术,测定了不同发酵时长(1~96个月)郫县豆瓣酱中色胺、β-苯乙胺、腐胺、尸胺、组胺、酪胺、亚精胺和精胺8种生物胺的含量。结果显示,HPLC分析中,8种生物胺的检出限在0.17~0.35 mg/L之间,定量限在0.55~0.92 mg/L之间,各生物胺标品在1~60 mg/L的质量浓度范围内线性关系良好,可被准确定量;各生物胺回收率在73.10%~104.13%之间,说明本方法具有较高的重复性和灵敏度。腐胺、尸胺、酪胺和组胺是郫县豆瓣酱中的主要生物胺;随着发酵时间的延长,腐胺含量快速上升,尸胺含量在发酵前54个月迅速上升,之后明显下降,酪胺和组胺在发酵54个月后含量有所增加;其他生物胺含量较低,且发酵期间变化不明显;郫县豆瓣酱中总生物胺含量随发酵时间的延长不断积累,从(37.86±6.69) mg/kg增加到(155.09±8.53) mg/kg,其含量在发酵前54个月快速上升,之后趋于稳定。目前食品中的多数生物胺尚没有限量标准,但参考现有的毒理学数据来看,本研究中郫县豆瓣酱的生物胺含量水平相对安全。     

Shelf life of several marine fish species of Bangladesh during ice storage

Organoleptic, biochemical and bacteriological aspects of five tropical marine fish such as silver jewfish, Bombay duck, big-eye tuna, Chinese pomfret and ribbon fish stored in ice were studied. Organoleptically all fish were found acceptable for 10–13 days before becoming inedible. Proximate analysis showed that moisture content increased slightly; protein and lipid content decreased gradually and ash had little or no significant change. pH, TVB-N, peroxide and aerobic plate count values increased with increasing storage period but the values were within acceptable range up to 10 days of storage. Changes in myofibrillar ATPase activity and solubility of these fishes showed that Ca²⁺ ATPase in the presence of both 0.1  m and 0.5  m KCl decreased gradually whereas no marked changes were observed in the Mg⁺² ATPase activities in the presence/absence of Ca⁺². Myofibrillar protein solubility of all fish samples also decreased considerably during ice storage. The decrement in Ca²⁺ ATPase activity and solubility indicates denaturation of myofibrillar protein of fish samples during ice storage.     

Effect of starter culture, spice mix and storage time and temperature on biogenic amine content of dry fermented sausages

Two types of dry fermented sausage differing in spicing mixture and the diameter (low content of red pepper + diameter 80 mm, H-sausage; high content of red pepper + diameter 55 mm, P-sausage, respectively) were produced in parallel with two different starter cultures (Pediococcus pentosaceus + Staphylococcus carnosus, B-samples and S. carnosus + Staphylococcus xylosus + Lactobacillus farciminis, F-samples, respectively). The sausages were ripened 21 days and subsequently stored 91 days at the room temperature. Concentration of both most abundant amines, putrescine and tyramine (y; mg/kg DM) increased significantly (P<0.01) in HB-sausage during ripening (x; days): y=2.5+18.13x−0.3144x² (R²=0.99) and y=0.7+8.17x−0.1130x² (R²=0.99), and also during storage: y=127.3+5.123x (R²=0.79) and y=26.0+3.211x (R²=0.74), respectively. At the end of ripening, putrescine (247 mg/kg DM) and tyramine (123 mg/kg DM) content in the HB-sausage was higher (P<0.05) than in the PB-sausage (12 and 9 mg/kg DM, respectively), concentration of either of these amines was negligible (1 mg/kg DM) in either type of F-inoculated sausage. Both starter culture and sausage type influenced significantly (P<0.001) both putrescine and tyramine content in the sausage; starter accounted for 57% and 55% of total variability in putrescine and tyramine content, respectively. Due to the significant (P<0.05) increase of total aerobic counts in the HB-sausage between the end of ripening and the 7th day of storage, followed by the significant (P<0.01) increase of the sum of total biogenic amines between the end of ripening (425 mg/kg DM) and the end of storage (1029 mg/kg DM), the storage of the dry fermented sausages at the room temperature should not be recommended.     

Biogenic amine content and biogenic amine quality indices of sardines (Sardina pilchardus) stored in modified atmosphere packaging and vacuum packaging

A comparative study of the effects of packaging on the formation of biogenic amines during storage of sardines (Sardina pilchardus) at 4 °C in air, modified atmosphere pack (MAP) and vacuum pack (VP) was carried out. Sardines were organoleptically acceptable for up to 3 days in air, 12 days in MAP and 9 days in VP. The biogenic amine content generally increased in all treatments with increasing storage time. The concentrations of putrescine and/cadaverine in fish stored in air reached maximum levels of 12.2 mg/100g at 12 days and 10.0 mg/l00 g at 15 days. Significant differences were found (P < 0.05) in the levels of cadaverine and putrescine among the three treatments. Spermidine and spermine levels increased slightly and did not change much throughout the storage period for all experimental conditions. The amine contents of sardine were highest in sardine stored in air, followed by VP and MAP. Quality indices related to the contents of the major biogenic amines were calculated and they correlated well with organoleptic qualities.     

Changes in free amino acids and biogenic amines of Egyptian salted-fermented fish (Feseekh) during ripening and storage

The aim of this study was to investigate changes in the formation of amino acids and biogenic amines in Egyptian salted-fermented fish (Feseekh) during ripening (20 days) and storage (40–60 days). The total concentration of free amino acids increased from 8 (dry weight; DW) to 72 g/kg (DW) after 60 days of storage. The predominant free amino acids were leucine, glutamic acid, lysine, alanine, valine, aspartic acid, isoleucine and citrulline. Their concentrations accounted for 68% of the total concentration of amino acids after 60 days. The total contents of biogenic amines ranged from 84 to 1633 mg/kg (DW) during the investigated period. Cadaverine was the major amine detected in Feseekh at all sampling stages and its concentration varied between 21 and 997 mg/kg (DW). The histamine content (211 mg/kg DW) only exceeded the maximum tolerance level (200 mg/kg) after 60 days. It could be concluded that Feseekh can be consumed without any health risks between 20 and 40 days but it can be hazardous after 60 days due to the biogenic amine content.     

不同贮藏温度下三文鱼鱼片生物胺和品质的变化

为了有效控制三文鱼的品质及质量安全,本文研究了25、4、0 ℃和?20 ℃贮藏温度下三文鱼鱼片生物胺和品质变化。将三文鱼鱼片分别贮藏于 25、4、0 ℃和?20 ℃,测定生物胺含量,菌落总数、TBA、pH及感官评分等鲜度指标。结果表明:三文鱼鱼片中主要生物胺为苯乙胺、腐胺、尸胺、酪胺和组胺,贮藏初期三文鱼鱼片中5种生物胺均处于较低水平;随着贮藏时间的延长,25、4和0 ℃贮藏下生物胺含量具有明显增加趋势,25 ℃贮藏末期5种生物胺含量分别增加了5.6、32.65、15.81、7.22倍和54.12倍;精胺和亚精胺在贮藏期间变化不明显;?20 ℃贮藏期间生物胺含量无明显变化,始终维持在较低水平。25、4和0 ℃贮藏下三文鱼鱼片的微生物及硫代巴比妥酸值呈现明显上升趋势,温度越高上升越快;感官评分呈下降趋势,且温度越低下降越慢;?20 ℃贮藏下各指标均优于25、4和0 ℃贮藏下三文鱼鱼片的新鲜度指标。温度对三文鱼鱼片在贮藏期间的生物胺变化影响明显,低温能够有效控制三文鱼中生物胺的产生并减缓品质劣化。     

Influence of different exopolysaccharide-producing strains on the physicochemical, sensory and syneresis characteristics of reduced-fat stirred yoghurt

Reduced-fat stirred yoghurts were prepared by using ropy and nonropy exopolysaccharide (EPS)-producing strains or nonexopolysaccharide-producing strains. Yoghurts were evaluated for pH, titrable acidity, acetaldehyde, whey separation, viscosity, EPS concentration, sensory and microbiological analyses. Determinations of these parameters were made at 1, 7, 14 and 21 days of storage. Reduced-fat yoghurts made with EPS⁺ strains exhibited lower syneresis and acetaldehyde contents, but higher viscosity than those made with the EPS ⁻ strain. Physico-chemical results correlated with the sensory results in that panellists scored the EPS⁺ yoghurts as having an overall better mouthfeel, but a worse flavour than EPS ⁻ yoghurt.     

Influence of hygienic quality of raw materials on biogenic amine production during ripening and storage of dry fermented sausages

The effect of the hygienic status of raw materials on biogenic amine production during ripening and storage (at 4 and 15 degrees C) of fermented sausages was studied. Two portions of fresh lean and back fat pork were stored for 5 days at -20 degrees C (treatment A) and at 4 degrees C (treatment B), respectively. Raw materials of treatment A maintained their hygienic quality high and low amine content. Raw materials of treatment B showed from 1 to 3 log (CFU/g) higher microbial counts and a biogenic amine index near 50 mg/kg, indicating poorer hygienic quality. The quality of raw materials influenced the composition and the concentration of biogenic amines produced during the ripening sausages. Sausages of treatment A (A-sausages) showed a large accumulation of tyramine (up to 100 mg/kg dm) followed by putrescine and cadaverine (<15 mg/kg). In contrast, B-sausages resulted in earlier and much greater amine production, and cadaverine, tyramine, and putrescine levels were 50-, 2.6-, and 6.5-fold higher than those of A-sausages. Other biogenic amines, such as octopamine, tryptamine, phenylethylamine, and histamine were also produced in B-sausages. The higher proteolysis and the lower pH of B-sausages might have favored the decarboxylase activity of microorganisms. Biogenic amine contents of sausages during storage depended on the raw materials used and storage temperature. No significant modification on the amine contents was observed during the storage of A-sausages at either temperature. Greater changes occurred in B-sausages stored at 15 degrees C than in those stored at 4 degrees C. Higher temperatures favored proteolytic and decarboxylase reactions, resulting in increased amine concentrations after storage.     

COMPARISON OF MARGIN OF SAFETY BETWEEN SENSORY SPOILAGE AND ONSET OF CLOSTRIDIUM BOTULINUM TOXIN DEVELOPMENT DURING STORAGE OF MODIFIED ATMOSPHERE (MA)-PACKAGED FRESH MARINE COD FILLETS WITH MA-PACKAGED AQUACULTURED FISH FILLETS

The margin of safety between shelf-life (onset of sensory spoilage) and potential time to toxin production by Clostridium botulinum type E in retail type packages of fresh marine cod fillets packaged in high barrier film under selected atmospheres [100% air, a modified atmosphere (MA) containing 75% CO₂:25% N₂, and vacuum] and stored under refrigeration (4C) and temperature-abuse conditions (8 and 16C) was investigated. Margin of safety data of MA-packaged marine cod fillets was also compared with MA-packaged aquacultured tilapia, catfish, and salmon fillets. Sensory spoilage preceded onset of toxin presence for the marine cod fillets packaged in any of the atmospheres and storage temperatures tested. At 4C, none of the marine cod fillets packaged in either atmosphere developed toxin, even 20 days after spoilage, as determined by sensory characteristics. During storage at refrigeration and mild (8C) temperature-abuse conditions, MA-packaged marine cod showed a greater margin of safety compared to aquacultured tilapia, catfish, and salmon packaged under the same atmospheres and conditions. Fat content appeared to potentially influence the margin of safety in MA-packaged aquacultured fresh fish fillets during storage.     

Shelf Life Assessment of Modified Atmosphere Packaged Turbot (Psetta maxima) Fillets: Evaluation of Microbial, Physical and Chemical Quality Parameters

Quality and safety of turbot fillets under modified atmosphere packaging (MAP) were assessed by microbial (total viable counts), physical (drip loss, pH, colour CIE Lab) and chemical parameters (total volatile basic nitrogen (TVBN), trimethylamine nitrogen (TMAN), biogenic amine contents). Three different atmospheres (MAP 1, 10/40/50 % O₂/CO₂/N₂; MAP 2, 10/60/30 % O₂/CO₂/N₂; MAP 3, 10/80/10 % O₂/CO₂/N₂) were tested. Packaged turbot fillets were stored at 2?±?1 °C and monitored over 30 days, at intervals of 5 days. Fillets from the control group, packaged with air (AIR), were the first to present signs of degradation reaching rejection threshold values for all evaluated parameters. In MAP fillets, total bacterial count was lower than 10⁶ cfu/g for a longer period. After 10 days of storage, MAP and AIR fillets showed significant differences (p?<?0.05) on the values of TVBN, TMAN and biogenic amines. MAP fillets presented a higher drip loss, and fillets in AIR became more yellowish (upper b* values) while those in MAP looked whitish for an increased period (upper L* values). All microbial, chemical and physical traits revealed the protective effect of the different MAP studied, especially those with a higher percentage of CO₂. MAP application added, at least, 5 days to shelf life of turbot fillets.