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1.
综述微生物发酵甘油生产1,3-丙二醇的研究进展,内容包括生产菌种、发酵方式、优良生产菌株的选育和培养条件,并展望1,3-丙二醇发酵的前景。 相似文献
2.
Yumi Lee 《木材化学与工艺学杂志》2016,36(5):353-364
Red pine wood, Pinus densiflora, biomass was liquefied through liquefaction using a solvent mixture of crude glycerol and PEG-400 with a sulfuric acid catalyst. The liquefaction process parameters of crude glycerol/PEG-400 blending ratio, biomass loading, acid loading, reaction temperature, and reaction time were optimized. Biopolyol with 61.9% biomass conversion was produced at 170°C within 1 h using a co-solvent of crude glycerol and PEG-400 (5/5, w/w), 15% biomass loading, and 3% sulfuric acid loading. The biopolyol possessed a 4.2 mg KOH/g acid number and 892.4 mg KOH/g hydroxyl number. Polyurethane foam was successfully synthesized from the liquefied red pine wood biomass with toluene diisocyanate. The synthesis of biopolyurethane derived from red pine wood biopolyol was confirmed with FT-IR. 相似文献
3.
Irina Lascu Ana Maria Tnase Piotr Jablonski Iulia Chiciudean Maria Irina Preda Sorin Avramescu Knut Irgum Ileana Stoica 《International journal of molecular sciences》2022,23(22)
Polyhydroxyalkanoates (PHA) are promising biodegradable and biocompatible bioplastics, and extensive knowledge of the employed bacterial strain’s metabolic capabilities is necessary in choosing economically feasible production conditions. This study aimed to create an in-depth view of the utilization of Photobacterium ganghwense C2.2 for PHA production by linking a wide array of characterization methods: metabolic pathway annotation from the strain’s complete genome, high-throughput phenotypic tests, and biomass analyses through plate-based assays and flask and bioreactor cultivations. We confirmed, in PHA production conditions, urea catabolization, fatty acid degradation and synthesis, and high pH variation and osmotic stress tolerance. With urea as a nitrogen source, pure and rapeseed-biodiesel crude glycerol were analyzed comparatively as carbon sources for fermentation at 20 °C. Flask cultivations yielded 2.2 g/L and 2 g/L PHA at 120 h, respectively, with molecular weights of 428,629 g/mol and 81,515 g/mol. Bioreactor batch cultivation doubled biomass accumulation (10 g/L and 13.2 g/L) in 48 h, with a PHA productivity of 0.133 g/(L·h) and 0.05 g/(L·h). Thus, phenotypic and genomic analyses determined the successful use of Photobacterium ganghwense C2.2 for PHA production using urea and crude glycerol and 20 g/L NaCl, without pH adjustment, providing the basis for a viable fermentation process. 相似文献
4.
生物发酵甘油生产1,3-丙二醇的自动化控制是其工业化应用亟待解决的关键问题。首先,采用数学函数连续性分析深入研究了克雷伯氏杆菌连续发酵甘油生产1,3-丙二醇过程的多稳态特性。在不同的初始甘油浓度或稀释速率下,系统均会出现多稳态现象,通过双因素分析确定了多稳态出现的临界区域,该区域内部的稳态是不稳定的。之后,基于反馈控制理论和多稳态分析结果,设计优化了受残余甘油和产物浓度影响的稀释速率控制策略。在连续发酵过程中,调整时间从81.27 h缩短到34.11 h,显著提高了发酵初期阶段甘油的利用率;同时甘油转化率由0.478 mmol·mmol-1提高至0.563 mmol·mmol-1,生产强度由85.70 mmol·L-1·h-1提高到101.10 mmol·L-1·h-1,显著提高了生产性能。 相似文献