Milk ejection in dairy cows at different degrees of udder filling.

Occurrence of milk ejection and course of milk removal were investigated in 18 dairy cows at milking intervals of 4, 8 and 12 h in early, mid or late lactation. Milk ejection occurred fastest in early lactation at a milking interval of 12 h and was delayed at short milking intervals and in late lactation. Storage capacity of the udder was estimated and the actual milk yields of experimental milkings were calculated as a percentage of storage capacity, i.e. degree of udder filling. It was shown that the occurrence of milk ejection after the start of teat stimulation is a function of udder filling. The relationship between the degree of udder filling and the delay from the start of milking until commencement of milk ejection followed a linear regression curve. Changes in occurrence and course of milk ejection have to be considered in practical milking, mainly in late stages of lactation and after short milking intervals. In automatic milking systems where variable and sometimes extremely short milking intervals occur, the duration of pre-milking udder preparation should be adapted to the expected milk yield at each individual milking procedure.     

The interplay of continuous milk ejection and milking system with and without prestimulation at different vacuum settings

Efficient machine milking requires an optimal interaction of alveolar milk ejection in the udder and milk removal by the milking machine. The aim of the present study was to test whether the equilibrium between continuous milk ejection and milk removal can also be maintained at very fast milking through a particularly high vacuum. Eight Holstein dairy cows were milked at 42, 52, or 60 kPa, with (PS) or without (nPS) prestimulation. Each of the 6 treatments was conducted at 2 afternoon milkings in each animal. The prestimulation lasted 40 s and consisted of forestripping and teat cleaning. The cluster attachment followed after a 20-s latency period. Throughout each milking, B-mode ultrasound videos of the gland cistern of 1 front quarter as well as milk flow and claw vacuum curves were recorded. Total milk yield was neither affected by nPS or PS nor by the vacuum level. Milk removed within the first minute and the first 2 min of milking and average milk flow were higher, and the duration of incline and time until peak milk flow were shorter at PS than at nPS milkings at all vacuum levels. Machine-on time was shorter at PS than at nPS milkings, although only at 42 and 52 kPa vacuum, obviously caused by the high percentage of bimodalities occurring in nPS milkings (17% bimodalities in PS vs. 92% bimodalities in nPS milkings). The frequency of bimodalities was higher at high than at low vacuum both in PS and nPS milkings. Peak flow rate and average milk flow were both higher at higher vacuum levels. The duration of milk flow plateau was shorter at 60 kPa than at 42 kPa milkings. At the highest vacuum (60 kPa), the shorter plateau phase indicated a declining milk ejection rate toward the end of the plateau phase, and milk ejection could no longer keep up with the fast milk removal; hence, a higher milking efficiency at a higher vacuum level could only be achieved as long as the gland cistern remained sufficiently filled by the continuous milk ejection. The ultrasound imaging confirmed this finding as the duration of cisternal area plateau in the recorded front quarter was shorter at high than at low vacuum. Thus, the highest vacuum of 60 kPa did not cause a shorter machine-on time than 52 kPa. In conclusion, milking at a very high vacuum can increase milking efficiency compared with a low vacuum. However, a vacuum reduction at the start and toward the end of milking is required to prevent overmilking if milking is performed at a very high vacuum.     

The effect of pre-milking teat-brushing on milk processing time in an automated milking system

Cow throughput in an automatic milking system (AMS) is limited by system parameters such as the time required for pre-milking udder preparation and cup attachment, physiological responses of the cow (such as milk let-down and milking-out rate), milking machine features and cow behaviour. A single-factor cross-over design was used to investigate the effect of pre-milking teat brushing on milk processing time in an AMS operating in an extensive grazing farming system. Teat brushing consisted of two roller brushes tracking up each teat three times (total brushing time of up to 45 s/cow). Cows were allocated to one of two treatment groups with either no brushing (NB) or brushing (B) for a 4-week period before being changed to the other treatment. Teat brushing resulted in shorter average cups-on-time (B = 506.1 s, NB = 541.0 s, P = 0.0001), longer average milk processing time (B = 10.30 min, NB = 9.76 min, P = 0.001) and no difference in daily milk yield (B = 14.67, NB = 14.71 kg/cow, P = 0.826). There was no difference between the two treatments in the success of cup attachment (B = 3.76%, NB = 5.10% unsuccessful milking attempts, P = 0.285). The estimated time cost of pre-milking teat brushing was 53 min for every 100 milkings, equivalent to an additional 5-6 milkings for every 100 milkings by an AMS. The importance of these potential time savings is discussed in relation to automatic milking in farming systems that aim for a lower per cow milking frequency and high ratio of cows to AMS.     

Effects of cleaning duration and water temperature on oxytocin release and milk removal in an automatic milking system

Four different methods of teat preparation during milking in an automatic milking system were studied in 2 experiments on Red Holstein/German Fleckvieh cross-breed cows. Milking routines used were milking: 1) without premilking teat preparation; 2) with one cleaning cycle (58 to 60 s) with cold (13 to 15 degrees C) water; 3) with one cleaning cycle with warm water (30 to 32 degrees C); or 4) with 2 cleaning cycles (122 s) with warm water. In experiment 1, milking characteristics were evaluated and milking routines were randomly assigned to 62 cows during 3 measuring periods of 24 h each. In experiment 2, 10 randomly selected cows were assigned to the same milking routines during 4 d and blood samples for oxytocin (OT) determination were taken during milking in addition to milk flow recording. Milk production, peak flow rate, total, and quarter milk yields showed no differences among treatments. Premilking preparation with cold water compared with warm water showed no differences in OT release, milk yield, peak flow rate, main milking time, average flow rate, or time until main milk flow. Baseline OT concentrations were consistently low. At the start of teat cup attachment without premilking teat preparation OT concentrations remained on the basal level but were elevated in all other treatments. By 30 s from the start of milking, OT concentrations were markedly increased in all treatments and were no longer different between treatments. In conclusion, the teat cleaning device used in the automatic milking system, either with warm or cold water, was suitable to induce milk ejection in cows before the start of milking.     

Individual differences in behavioral and physiological responsiveness of primiparous dairy cows to machine milking

An experiment was performed in primiparous dairy cows (n = 23) to examine consistency of individual differences in reactivity to milking, and correlations between measures of behavior, physiology, and milk ejection. Responsiveness to milking was monitored during the first machine milking, on d 2 of lactation, and during milkings on d 4 and 130 of lactation. Measurements included kicking and stepping behavior, plasma cortisol and plasma oxytocin, heart rate, milk yield, milking time, milk flow rate, and residual milk obtained after administration of exogenous oxytocin. With repeated early lactation milkings, residual milk and the incidence of abnormal milk flow curves decreased. On d 130 of lactation all heifers exhibited normal milk ejection. Except for higher plasma cortisol concentrations on d 2, all measures were consistent over time between d 2 and 4 of lactation as indicated by significant rank correlations. Individual differences in the behavioral response to udder preparation were consistent over time between early lactation milkings and d 130 of lactation. Residual milk, milk yield, maximum milk flow rate, plasma oxytocin and heart rate during udder preparation were similarly interrelated on d 2 and 4 of lactation. High heart rate responses on d 2 and 4 were associated with enhanced inhibition of milk ejection. In contrast, behavior recorded during the milking process was unrelated to ease of milk removal. Our results indicate that milking at the beginning of lactation may be stressful to some heifers, to the extent that milk ejection is inhibited, but less disturbing to others. The existence of consistent behavioral and physiological responses in the present study suggests that responsiveness of dairy heifers to milking is mediated by stable animal characteristics.     

Reduced liner-open phase and vacuum instead of prestimulation increase parlor efficiency in dairy cows

Adequate prestimulation is considered a requirement for a fast, gentle, and complete udder emptying at machine milking. Reduced vacuum or reduced liner-open phase of pulsation (or both) may replace prestimulation and consequently reduce work load of the milker and increase parlor efficiency. In the present study we compared 2 milking routines (MR) with manual prestimulation (LPrep = long preparation: 15 s of forestripping, teat cleaning, and stimulation; SPrep = short preparation: 5 s of teat cleaning) followed by 1-min latency period and milking at standard vacuum and pulsation settings (claw vacuum 44 kPa, pulsation rate 60 cycles/min, pulsation ratio 65/35) with 2 MR consisting of 5 s of teat cleaning, immediate cluster attachment and milking at reduced vacuum with or without shortened liner-open phase of pulsation until milk flow exceeded 400 g/min (RP = reduced pulsation: pulsation ratio 30/70, pulsation rate 50 cycles/min, claw vacuum 44 kPa; RPV = reduced pulsation and vacuum: pulsation ratio 30/70, pulsation rate 50 cycles/min, claw vacuum 34 kPa). Cluster detachment was performed at 400 g/min in all MR. Ten Holstein dairy cows were milked twice daily at 14-h and 10-h milking intervals. Milk flow and electrical conductivity (EC) were recorded throughout milking. During the first 2 min of each milking ultrasound cross-section images of the gland cistern of one front quarter were recorded, and 5 min after the end of milking teat tissue thickness of both front teats was measured by using a cutimeter. Most milking characteristics such as total milk yield, average milk flow, and machine-on time reached higher values at 14-h than 10-h milking intervals, but did not differ among MR. However, the occupancy time (time from the first touch of the udder until cluster detachment) was considerably shorter in RP and RPV compared with LPrep and SPrep. Ultrasound cross section areas of the gland cistern were larger in LPrep than in RP and RPV indicating that milk ejection already occurred at cluster attachment in LPrep. This assumption is also supported by the lower EC at cluster attachment in LPrep than in RP and RPV, which was caused by the presence of alveolar milk in the gland cistern after milk ejection. The MR RP and RPV increase parlor efficiency and are work-saving alternatives to MR, which include an adequate prestimulation (LPrep). However, shortening prestimulation to a 5-s teat cleaning followed by a latency period and milking at regular vacuum and pulsation is not adequate to save occupancy time. Because milking was performed at a relatively low vacuum (44 kPa) and at a detachment level of 400 g/min, teat tissue thickness did not differ among MR, and the vacuum reduction in RPV did not cause an additional advantage for teat condition compared with RP.     

Effects on milk yield of milking interval regularity and teat cup attachment failures with robotic milking systems

A database consisting of 35291 milking records from 83 cows was built over a period of 10 months with the objectives of studying the effect of teat cup attachment failures and milking interval regularity on milk production with an automated milking system (AMS). The database collected records of lactation number, days in milk (DIM), milk production, interval between milkings (for both the entire udder and individual quarters in case of a teat cup attachment failure) and average and peak milk flows for each milking. The weekly coefficient of variation (CV) of milking intervals was used as a measure of milking regularity. DIM, milking intervals, and CV of milking intervals were divided into four categories coinciding with the four quartiles of their respective distributions. The data were analysed by analysis of variance with cow as a random effect and lactation number, DIM, the occurrence of a milking failure, and the intervals between milkings or the weekly CV of milking intervals as fixed effects. The incidence of attachment failures was 7.6% of total milkings. Milk production by quarters affected by a milking failure following the failure was numerically greater owing to the longer interval between milkings. When accounting for the effect of milking intervals, milk production by affected quarters following a milking failure was 26% lower than with regular milkings. However, the decrease in milk production by quarters affected by milking failures was more severe as DIM increased. Average and peak milk flows by quarters affected by a milking failure were lower than when milkings occurred normally. However, milk production recovered its former level within seven milkings following a milking failure. Uneven frequency (weekly CV of milking intervals >27%) decreased daily milk yield, and affected multiparous more negatively than primiparous cows.     

Effects of duration of udder stimulation on milking dynamics and oxytocin release

For effects of varying duration of premilking manual udder massage on milking performance and oxytocin release, five Holstein cows were subjected to a) no stimulation, b) stimulation by manual udder massage before machine attachment for 15 s, c) for 30 s, d) for 60 s, and e) for 120 s. Milk yield, fat, protein, and somatic cell content were not affected by treatments. The 30-, 60-, and 120-s treatments resulted in higher peak milk flow rate as compared to no stimulation. Stimulation for 60 or 120 s did not achieve higher peak milk flow rates than 15 or 30 s. Average milk flow rate increased with duration of stimulation, and no stimulation differed from 30-, 60-, and 120-s treatments. Machine-on time decreased with increasing stimulation. However, differences in average flow rates and machine-on times became nonsignificant when premilking stimulation time was added to machine-on time. No differences were significant between treatments and mean oxytocin concentrations. The marked difference in milk flow variables and machine-on time suggested a small yet finite threshold concentration of oxytocin leads to milk ejection. Udder massage of 30 s prior to machine attachment is more than adequate for preparing cows for milking with machine stripping.     

Relationship among udder and teat morphology and milking characteristics.

Teat cup liner slips, manual milking machine adjustments, milk yields, and milking times were recorded during both morning and evening milkings for 8 d on 97 Holstein cows in The Pennsylvania State University dairy herd. Fore and rear udder heights (distance from floor to udder), udder levelness, distances between teats (before and after milking), teat lengths, teat diameters, and teat end shapes were measured on the same cows. Product-moment correlations among the morphological characteristics, linear slips, manual adjustments, milk yields, and milking times were determined. Residual correlations from a model including lactation number and DIM (linear and quadratic) were also calculated. The variation among cows in machine liner slips and manual adjustments within and across lactation number and DIM can be partially explained by udder and teat morphology. Wider teats were associated with increased linear slips and increased manual adjustments. More tilted udders (rear quarters lower than front quarters) were associated with increased liner slips and tended to be associated with increased manual adjustments. In addition, larger teat diameters and longer teats tended to be associated with increased liner slips.     

Effect of milking interval on alveolar versus cisternal milk accumulation and milk production and composition in dairy ewes

Cisternal and alveolar milk fractions were measured in East Friesian crossbred dairy ewes (n = 32) after 4, 8, 12, 16, 20, or 24 h of milk accumulation in a 6 x 6 Latin square design by administration of an oxytocin receptor antagonist for recuperation of cisternal milk followed by injection of oxytocin to remove the alveolar fraction. Less than half (38 to 47%) of the total milk yield was stored within the cistern for the first 12 h of udder filling compared with up to 57% after 24 h of udder filling. Subsequent milk yield was significantly reduced following the 16-, 20-, and 24-h treatments. Cisternal milk fat percentage, but not milk protein percentage, was lower than in alveolar milk (4.49 vs. 7.92% milk fat, respectively), indicating that casein micelles pass more freely from the alveoli to the cistern between milkings compared with fat globules. Alveolar compared to cisternal somatic cell count was higher for the 16-, 20-, and 24-h treatments. Significant increases in cisternal milk yield and milk composition observed for the 24-h compared with the 20-h treatment demonstrated the importance of the cistern as a storage space when the alveoli and small intramammary ducts became full. The main difference between cisternal and alveolar milk fractions is the poor fat content of cisternal milk, which is an important reason for the milk ejection reflex to be present during machine milking of dairy ewes. In a second experiment, milking every 16 h compared with every 12 h during mid- to late-lactation did not effect milk yield, milk composition, and quality, or lactation length; however, a 25% savings in labor was achieved with the longer milking interval.     

Milk production, health, behavior, and endocrine responses of cows exposed to electrical current during milking

Six cows were exposed during milkings to electrical current to assess its effects on behavior, health, milking performance, and endocrine responses. Three treatments (0, 4, and 8 mA) were applied in a changeover design over three consecutive 1-wk periods. A cow received the same current treatment during 14 consecutive milkings, beginning with the evening milking (d 1) and ending with the morning milking (d 8). Treatments began 5 min before milking and continued until milking unit removal. Treatments consisted of 60 Hz square wave current of 5-s duration applied every 30 s from udder to hooves. Milk accumulation curves provided information about milk yields, milking times, peak milk flow rates, and times of peak milk flow. Residual milk yields also were measured. Milk was analyzed for protein, fat, and somatic cells. Blood samples from 60 min before to 60 min after treatment were collected, and oxytocin, prolactin, and cortisol concentrations were measured. Behavioral responses to current decreased with time. Changes of milking performance and milk composition were not significant. Changes of milking related cortisol responses during 8-mA current stimulation were significant. Oxytocin release was delayed during 8-mA treatments. Current treatments did not affect prolactin.     

Optimization of individual prestimulation in dairy cows

The application of prestimulation results in enhanced milking performance compared with milking without prestimulation. In the present study oxytocin (OT) release and milking characteristics were investigated in 43 dairy cows after the application of various prestimulation routines by vibration stimulation lasting between 0 and 90 s. Additionally, different maximum pulsation vacuum settings during vibration stimulation were investigated. The actual degree of udder fill was calculated as a percentage of the estimated storage capacity. The amplitude of OT release, total milk yield, and stripping milk yield did not differ between prestimulation routines. Increased maximum pulsation vacuum during vibration stimulation resulted in milk flow during prestimulation, but did not negatively influence milking characteristics. The lag time from the start of teat stimulation until the start of milk ejection was negatively correlated with the degree of udder fill. This relationship was the reason for variations in optimal duration of prestimulation. The optimal duration of prestimulation to receive immediate and continuous milk flow at the start of milking was 90 s in udders containing small amounts of milk, whereas the optimal duration was only 20 s in well-filled udders. A short prestimulation enhances milking stall capacity when milking full udders, and a prolonged prestimulation reduces the total vacuum load on the teat when milking udders that are not full.     

Technical note: Reduced pulsation chamber vacuum at normal pulsation rate and ratio provides adequate prestimulation to induce oxytocin release and milk ejection while simultaneous milk flow is prevented

In the present study we investigated the milking characteristics and the oxytocin release in dairy cows milked after either manual prestimulation or a premilking period with pulsating liners at normal pulsation rate (60) and ratio (60:40) while the pulsation chamber vacuum (PCV) was reduced to 20 kPa to prevent the opening of the liners. During the milking trial with 8 cows the PCV reduction was started either before attachment (PCV-1) or immediately after attachment (PCV-2) of the teat cups. Milk yields, total milking times, average milk flows, peak flow rates, the duration of milk flow plateaus, and the duration of milk flow declines did not differ among the 3 treatments. Only the time to reach peak milk flow was prolonged when the vacuum reduction was started after teat cup attachment (PCV-2). In this treatment, milk flow >200 g/min already occurred during the premilking period, resulting in bimodal milk flow curves. In 5 of the 8 cows, plasma oxytocin (OT) concentrations were measured from ?2 min before the start of milking until 3 min of milking to compare the OT release in response to manual prestimulation and during PCV-1. In both treatments, OT increased similarly and remained elevated until the end of measurements. Consequently, the areas under the curve of OT concentrations did not differ between treatments. In conclusion, milking performance is similar if milking is performed after manual prestimulation or after normal pulsation at reduced PCV. To prevent milk flow during the prestimulation period, it is of crucial importance to start the reduction of the PCV before cluster attachment.     

Effect of different milking routines on milking-related release of the hormones oxytocin, prolactin and cortisol, and on milk yield and milking performance in Murrah buffaloes

Milking-related release of oxytocin, prolactin, and cortisol was studied following three premilking treatments. Six Murrah buffaloes were treated with direct application of milking cluster (O), a 1-min pre-stimulation (M), and combined feeding and pre-stimulation (MF). Machine milk yield, stripping yield and milk composition were recorded. Milk ejection occurred significantly earlier with MF than M and O (P<0.05; 2.50, 5.10 and 6.33 min, respectively). In all treatments, milk ejection occurred with small increases >3-5 ng/l in oxytocin concentration. Increase in oxytocin concentration over a threshold level and milk ejection occurred simultaneously and were closely correlated (r=0.83, P<0.05). There was a positive correlation between total time oxytocin concentration remained elevated over threshold levels and machine yield (r=0.86, P<0.05). For treatment O, milk ejection was inhibited during machine milking, while a marked increase in oxytocin occurred during hand stripping (6 and 16 ng/l, respectively). For treatment M, mean oxytocin concentrations remained unchanged during prestimulation but increased during subsequent machine milking and hand stripping (6.38, 18.06 and 12.36 ng/l, respectively). For treatment MF, although there was a 3.6-fold increase during pre-stimulation, oxytocin increased by 10-fold and 3-fold during machine milking and hand stripping, and was significant for machine milking (P<0.05, 17.32, 47.86, 18.13 ng/l, respectively). Milk-ejection-related cortisol release was visible only in treatment MF. For treatments O and M, prolactin concentration increased prior to the increase in oxytocin. The stripping yield was higher, and fat content in the stripping yield significantly lower, for treatment O indicating incomplete milking. Thus buffaloes are easily disturbed even by small changes in milking routines.     

Risk factors for delayed milk ejection in Holstein dairy cows milked 3 times per day

Delayed milk ejection, manifested most often as bimodal milk flow, occurs when the cisternal milk fraction is removed before the alveolar milk reaches the gland cistern. It is thought to be a consequence of not meeting cows' physiological needs, due to insufficient premilking teat stimulation, inadequate timing of milking unit attachment, or both. It has been associated with decreased milking efficiency, reduced milk yield, and impaired teat and udder health. Traditionally, portable electronic milk meters have been used to assess the presence of delayed milk ejection in dairy cows. By contrast, incremental milk flow rates from on-farm milk meters and their suitability as a measure to assess delayed milk ejection have not been studied by rigorous methods. The objectives were (1) to describe a protocol for identification of cows with chronically delayed milk ejection (CDME) and (2) to investigate risk factors for CDME using incremental milk flow rates obtained from automated on-farm milk meters. In a retrospective case control study, milk flow data from a 4,300-cow dairy with a thrice-daily milking schedule were obtained over a 1-wk period. Incremental milk flow rates (0–15 s, 15–30 s, 30–60 s, and 60–120 s) were used to identify cows with delayed milk ejection. Cases of CDME were defined as presence of delayed milk ejection at all 21 milking observations. Cows that had no delayed milk ejection at any of the same 21 milking observations were included as controls. A total of 171 cases and 393 controls were included in the study based on these criteria. A logistic regression model was used to evaluate associations of the following risk factors with CDME: parity (1, 2, ≥3), stage of lactation (<100, 101–200, >200 DIM), presence of a nonlactating quarter, milk somatic cell count, average daily milk production, and health and management events. Parity and CDME were associated such that compared with cows in their third or greater lactation, the odds (95% confidence intervals, 95% CI) of CDME were 1.27 (0.71–2.25) for cows in their first and 4.77 (2.47–9.22) for animals in their second lactation. The odds of CDME increased with increasing stage of lactation, with an odds ratio of 0.20 (0.11–0.36) for early and 0.28 (0.15–0.52) for mid-lactation animals, respectively, compared with late lactation cows. A 1-kg increase in average daily milk production was associated with decreased odds of CDME [odds ratio (95% CI): 0.89 (0.87–0.92)]. A lameness event during the study period increased the odds of CDME [odds ratio (95% CI): 8.04 (1.20–53.83)], as did a vaccination event 1 wk before the study period [odds ratio (95% CI): 4.07 (0.99–16.71)]. This study confirmed associations between CDME and previously reported risk factors and identified several previously less rigorously investigated health and management events that could be associated with CDME. Incremental milk flow rates from individual cows serve as an automated tool to evaluate milk flow dynamics. This information could be used to improve individual premilking udder preparation to meet the animal's physiological requirements, improve teat and udder health, and enhance parlor efficiency.     

Release of oxytocin in the cow during milking.

Jugular vein oxytocin concentrations were determined at 15-s intervals on 10 Holstein cows during milking. Oxytocin ranged from .5 muU/ml plasma prior to udder washing to 652.7 muU/ml plasma at 1 min after application of teat cups. The results indicate a gradually increasing release of oxyctocin after stimulation and a maximal oxytocin release 1.5 min after start of udder washing and 1 min after teat cup application.     

Aetiology of disturbed milk ejection in parturient primiparous cows.

Milk flow in nine primiparous cows with disturbed milk ejection (D) and in six corresponding control animals (C) with normal milk removal was recorded during machine milking and blood samples were taken before and during milking to determine plasma oxytocin, vasopressin, prolactin, cortisol, oestradiol-17 beta, luteinizing hormone, progesterone and beta-endorphin concentrations. Manual teat stimulation before milking lasted for 1 min. After milk flow had stopped, air was blown into the vagina for 2 min. When milk flow had stopped again, 1 i.u. oxytocin and finally 10 i.u. oxytocin were injected to remove residual milk. During and after teat stimulation, oxytocin remained basal in D, but increased in C, whereas prolactin increased in both groups. While 94% of total milk was obtained in C during this period, only 9% could be removed from D, indicating lack of alveolar milk ejection. During vaginal stimulation, oxytocin increased transiently in D and more than by teat stimulation in C. This allowed the removal of 75% of milk in D, whereas almost no more milk was available in C. After oxytocin injections, 3 and 16% of residual milk were obtained in C and D respectively. Basal oestradiol-17 beta concentration was higher in D than in C (11.6 and 2.0 ng/l respectively), whereas beta-endorphin level was lower (24.1 and 86.6 micrograms/l respectively). Basal concentration of luteinizing hormone and progesterone, and concentration of cortisol and vasopressin before and during milking were comparable in C and D. We conclude that in cows with disturbed milk ejection afferent nervous pathways to the hypothalamus were intact, because prolactin was released by teat stimulation. However, oxytocin was only released by vaginal stimulation, i.e. milk ejection was centrally inhibited during teat stimulation.     

Technical note: Effects of attachment of hind teats before cleaning and attachment of front teats on milking characteristics in automatic milking systems

Milking characteristics differ between the 4 quarters of a dairy cow udder. In particular, milking time is mostly prolonged in hind quarters compared with front quarters because of the usually higher amount of stored milk. The standard milking routine (STDMR) in both conventional and automatic milking systems (AMS) consists of teat preparation of all 4 quarters, followed by attachment of the 4 teat cups, regardless of the distribution of milk between quarters. In the current study, an alternative teat preparation and milking routine (ALTMR) in AMS was tested, which consisted of cleaning and starting the milking of hind teats before cleaning and attachment of front teats. The hypothesis was based on the fact that hind quarters have usually a longer milking time than front quarters. Starting the milking of hind quarters while the front teats are being cleaned may reduce the difference in the end of milking between front and hind quarters and thus reduce total milking time. Both routines were tested on 5 Swedish dairy farms equipped with AMS in a 4-wk experiment in which treatments were alternated weekly. Total milk yield did not differ between treatments. Machine-on time (MOT) was longer in ALTMR than in STDMR because the difference in milking time between hind and front quarters was less than the time needed to prepare the front teats. However, the longer MOT in ALTMR was compensated by a shorter total preparation time, including the attachment of the first teat cup, as only the hind teats (instead of all 4 teats) were cleaned before milking was started. This resulted in a similar total milking time from start of cleaning of the first quarter until the end of milking of the last quarter in both treatments. Because of the prolonged MOT, average milk flow rate was lower in ALTMR than STDMR. Peak flow rate was higher in ALTMR than STDMR, but only in teat cups 1 (first attached, hind quarter) and 3 (third attached, front quarter), whereas main milk flow was higher in ALTMR than STDMR in both front quarters. In conclusion, splitting teat cleaning and the start of milking between hind and front quarters does not prolong total milking time, including teat cleaning. The partially positive effect on peak and main milk flow indicates that the ALTMR is a suitable milking routine in AMS. In herds with a greater difference of milk stored in hind compared with front quarters, a reduced total milking time can be expected for ALTMR.     

Effect of weaning system on milk composition and distribution of milk fat within the udder of East Friesian dairy ewes

We investigated whether the inhibition of milk ejection during and/or between machine milkings is responsible for the low milk fat observed in commercial milk obtained from dairy ewes managed with a mixed system (MIX) of partial daily suckling (10 h) and once daily machine milking (after 14 h of udder filling). East Friesian crossbred dairy ewes were randomly allocated postpartum to the MIX system (n = 9) or to exclusive twice-daily machine milking (DY1, n = 8). Following wk 4, MIX ewes were permanently weaned from their lambs and milked twice daily. All ewes were injected with saline, oxytocin, or an oxytocin-receptor antagonist prior to three morning milkings during wk 2,4, and 6 of lactation to study cisternal and alveolar milk distribution. Overall milk yield (cisternal + alveolar) for MIX ewes was 42% greater than for DY1 ewes during wk 2 and 4, which demonstrates the beneficial effect of lamb suckling on milk production of dairy ewes. However, during normal machine milking, only the cisternal fraction was obtained from MIX ewes, confirming that milk ejection did not occur for as long as these ewes remained in partial daily contact with their lambs. Although the volume of milk stored within the cistern, and its concentration of milk protein was similar for the two weaning systems, milk of MIX ewes was significantly inferior in cisternal milk fat concentration and yield compared to DY1 ewes. This provides evidence that not only is there inhibition of milk ejection during machine milking of MIX ewes, there is additional inhibition of transfer of milk fat, but not milk protein, from the alveoli to the cistern during the evening when MIX ewes a reseparated from their lambs. Following weaning of MIX ewes, the majority of lactation traits studied were similar compared to DY1 ewes.     

Effect of milk flow rate switch-point settings on cow comfort and milking duration

Automatic cluster removers (ACR) operate by ceasing vacuum to the cluster and detaching the milking unit from the udder by means of a retracting cord once the milk flow has decreased to a predefined level (i.e., the milk flow rate switch-point). There is a large body of literature on this topic indicating that increasing the flow rate switch-point (e.g., from 0.2 kg/min to 0.8 kg/min at the udder level) is effective in reducing milking duration while having little effect on milk yield or milk somatic cell count (SCC). However, despite these findings many farms still use a switch-point of 0.2 kg/min because it is believed that emptying the udder completely at each milking is a prerequisite for good dairy cow management, especially in relation to maintaining a low milk SCC. However, there may be additional undocumented benefits in terms of cow comfort to increasing the milk flow rate switch-point, because the low milk flow period at the end of milking is a high-risk time for inducing teat-barrel congestion. The objective of this study was to quantify the effect of 4 milk flow rate switch-point settings on cow comfort, milking duration, and milk yield. In this study, we applied 4 treatments consisting of different milk flow rate switch-points to cows in a crossover design in a spring calving grass based dairy herd in Ireland. The treatments were (1) MFR0.2, where the cluster was removed at a milk flow rate of 0.2 kg/min; (2) MFR0.4, where the cluster was removed at 0.4 kg/min; (3) MFR0.6, where the cluster was removed at 0.6 kg/min, and (4) MFR0.8, where the cluster was removed at 0.8 kg/min. Milking parameters were recorded by the parlor software and leg movements (i.e., kicks or steps) during milking were recorded with an accelerometer. These data were used as a proxy for cow comfort during milking. The results of this study showed significant differences in cow comfort across treatments, as indicated by cow stepping during milking, for a.m. milkings, but these differences were not detected for p.m. milkings, possibly because a.m. milkings were longer than p.m. milkings due to a 16:8 h milking interval on the research farm. Differences tended to distinguish the 2 lower-flow switch-point settings with greater leg movement against the 2 higher-flow switch-point settings with less leg movement during milking. The effect of treatment (milk flow rate switch-point) on daily milking duration was significant. The milk duration for MFR0.8 was 89 s (14%) shorter than MFR0.2. There was no significant effect of treatment on SCC in this study.