Characterization of tricalcium phosphate solubilization by Stenotrophomonas maltophilia YC isolated from phosphate mines

The phosphate solubilizing characteristics of a strain YC, which was isolated from phosphate mines (Hubei, China), were studied in National Botanical Research Institute’s phosphate (NBRIP) growth medium containing tricalcium phosphate (TCP) as sole phosphorus (P) source. The strain YC is identified as Stenotrophomonas maltophilia (S. maltophilia) based upon the results of morphologic, physiological and biochemical characteristics and 16S rRNA sequences analysis. The results show that the strain S. maltophilia YC can solubilize TCP and release soluble P in NBRIP growth medium. A positive correlation between concentration of soluble P and population of the isolate and a negative correlation between concentration of soluble P and pH in the culture medium are observed from statistical analysis results. Moreover, gluconic acid is detected in the culture medium by HPLC analysis. It indicates that the isolate can release gluconic acid during the solubilizing experiment, which causes acidification of the culture medium and then TCP solubilization. S. maltophilia YC has a maximal TCP solubilizing capability when using maltose as carbon source and ammonium nitrate as nitrogen source, respectively, in NBRIP growth medium. Foundation item: Project(2004CB619201) supported by the Major State Basic Research and Development Program of China; Project(Z200515002) supported by the Key Project Foundation of the Education Department of Hubei Province, China; Project(GCP200801) supported by the Open Research Fund of Key Laboratory for Green Chemical Process of Ministry of Education, China; Project(Q200811) supported by the Youths Science Foundation of Wuhan Institute of Technology, China     

Bioleaching of sphalerite by Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans cultured in 9K medium modified with pyrrhotite

Elective culture of Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans in 9K medium modified with pyrrhotite was studied. Bioleaching of flotation concentrate of sphalerite by the selected bacteria was carried out. The results show that the microorganisms cultured by pyrrhotite are a mixture of Acidithiobacillusferrooxidans and Acidithiobacillus thiooxidans, of which the capability to oxidize ferrous to ferric irons is enhanced by the high mass ratio of Fe to S in pyrrhotite. Three pyrrhotite samples were separated into various parts with corresponding S/Fe ratios by magnetic separation and were used to culture the elective bacteria as the substrate. The association of the cultures could provide a more rapid and complete oxidation of sphalerite than that of bacteria cultivated by conventional methods.     

Isolation of Leptospirillum ferriphilum by single-layered solid medium

According to physiological and biochemical characteristics of Leptospirillum ferriphilum,a strain of object bacteria was isolated successfully.Bacteria were enriched by selective liquid medium and plated on designed single-layered agar solid medium.Colony was cultured and bacteria were collected.The morphologies of the object bacteria were observed using crystal violet staining,scanning electron microscope(SEM)and transmission electron microscope(TEM).The result of 16S rDNA identification shows that this bacterium belongs to Leptospirillum ferriphilum and it is named as Leptospirillum ferriphilum strain D1.These results indicate that this new single-layered agar solid medium is efficient and simple for isolation of Leptospirillum ferriphilum.Additionally,physiological-biochemical characteristics show that the optimum initial pH value and its growth temperature are 1.68 and 40 ℃,respectively.The culture of it is used to leach a complex concentrate chalcopyrite,the leaching efficiencies of copper and iron are 1.93 % and 13.74 %,respectively,and it is more effective than the A.ferrooxidans culture in the leaching of the complex concentrate chalcopyrite.     

Isolation and characterization of YNTC-1, a novel Alicyclobacillus sendaiensis strain

A heterotrophic acidothermophilic bacterial strain, YNTC-1, was isolated from an acidic hot spring in Tengchong, Yunan, China. YNTC-1 grows at pH value of 1.5-8.0 and temperature of 40-70 ℃, with optimal pH and temperature at 3.0 and 55 ℃, respectively. The cells of the strain are in shape of short rod, with 1.0-1.2 μm in length and 0.7-0.8 μm in diameter, and with distinct spores at both poles of each cell. The predominant fatty acids in cellular membrane of the strain are C18：1 ω7c. 16s rRNA gene analysis reveals that this strain is closely related to Alicyclobacillus sendaiensis, with over 99% sequence similarity. Based on phenotypic and genotypic analyses, YNTC-1 is identified as a member ofA. sendaiensis. Considering some important morphological and biochemical differences between strain YNTC-1 and A. sendaiensis ATCC 27009T, YNTC-1 may be proposed to be a novel subspecies of A. sendaiensis. However, this viewpoint has to be confirmed by further studies. Co-bioleaching of pyrite and chalcopyrite with strain YN22, Sulfobacillus thermosulfidooxidans, shows that strain YNTC-1 has no evident influence on bioleaching rates of these two sulphide minerals.     

Residual stresses within sprayed coatings

Some important developments of residual stress researches for coating-based systems were studied. The following topics were included the sources of residual stresses in coatings: error analysis of Stoney’s equation in the curvature method used for the measurement of coating residual stress, the modeling of residual stress and some analytical models for predicting the residual stresses in coatings. These topics should provide some important insights for the fail-safe design of the coating-based systems. Foundation item: Project(50235030) supported by the National Natural Science Foundation of China; Project(2003AA331130) supported by the National High-Tech Research and Development Program of China     

Screening, identification and desilication of a silicate bacterium

The strain Lv（1- z） isolated from the Henan bauxite was characterized by morphological observation, biochemical and physiological identification, and 16S rDNA sequence analysis. The influences of temperature, initial pH value, the volume of medium, shaking speed and illite concentration on the desilicating ability of the strain Lv（1- z） were investigated. The results show that the bacterium is a Gram-negative rod-shaped bacterium with oval endspores and thick capsule, but without flagellum. The biochemical and physiological tests indicate that the strain Lv（1- z） is similar to Bacillus rnucilaginosus. In GenBank the 16S rDNA sequence similarity of the strain Lv（1- z） and the B. rnucilaginosus YNUCC0001（AY571332） is more than 99%. Based on the above results, the strain Lv（1- z） is identified as B. rnucilaginosus. The optimum conditions for the strain L（1- z） to remove silicon from illite are as follows., temperature is 30℃ ;initial pH value is 7.5; medium volume in 200 mL bottle is 60 mL; shaking speed of rotary shaker is 220 r/m ; illite concentration is 1%.     

Effect of homogenization treatment on microstrucmre and properties of Al-Mg-Mn-Sc-Zr alloy

The effect of homogenization on the hardness, tensile properties, electrical conductivity and microstructure of as-cast Al-6Mg-0.4Mn-0.25Sc-0.12Zr alloy was studied. The results show that during homogenization as-cast studied alloy has obviously hardening effect that is similar to aging hardening behavior in traditional Al alloys. The precipitates are mainly Al3（Sc,Zr） and Al6Mn When homogenization temperature increases the hardness peak value is declined and the time corresponding to hardness peak value is shortened. The electrical conductivity of the alloy monotonously increases with increasing homogenization temperature and time. The decomposition of the supersaturated solid solution containing Sc and Zr which is formed during direct chilling casting and the precipitation of Al3（Sc, Zr） cause hardness increasing. The depletion of the matrix solid solubility decreases the ability of electron scattering in the alloy, resulting in the electrical conductivity increased. Tensile property result at hot rolling state shows that the optimal homogenization treatment processing is holding at 300-350℃ for 6-8 h.     

Deformation behavior and microstructural evolution of Al-Zn-Cu-Mg-Sc-Zr alloy during high temperature compression

The deformation behavior of a new Al-Zn-Cu-Mg-Sc-Zr alloy was investigated with compression tests in temperature range of 380–470 °C and strain rate range of 0.001–10 s⁻¹ using Gleeble 1500 system, and the associated microstructural evolutions were studied by metallographic microscopy and transmission electron microscopy. The results show that true stress—strain curves exhibit a peak stress, followed by a dynamic flow softening at low strains (ɛ<0.05). The stress decreases with increasing deformation temperature and decreasing strain rate, which can be represented by a Zener-Hollomon exponential equation with the activation energy for deformation of 157.9 kJ/mol. The substructure in the deformed specimens consists of few fine precipitates with equaixed polygonized subgrains in the elongated grains and developed serrations at the grain boundaries. The dynamic flow softening is attributed mainly to dynamic recovery and dynamic recrystallization. Foundation item: Project(2006AA03Z523) supported by the National High-Tech Research and Development Program of China     

A sink moving scheme based on local residual energy of nodes in wireless sensor networks

In the application of periodic data-gathering in sensor networks, sensor nodes located near the sink have to forward the data received from all other nodes to the sink, which depletes their energy very quickly. A moving scheme for the sink based on local residual energy was proposed. In the scheme, the sink periodically moves to a new location with the highest stay-value defined by the average residual energy and the number of neighbors. The scheme can balance energy consumption and prevent nodes around sink from draining their energy very quickly in the networks. The simulation results show that the scheme can prolong the network lifetime by 26%–65% compared with the earlier schemes where the sink is static or moves randomly. Foundation item: Project(60673164) supported by the National Natural Science Foundation of China; Project(20060533057) supported by the Specialized Research Foundation for the Doctoral Program of Higher Education of China     

Electron bandstructure of kaolinite and its mechanism of flotation using dodecylamine as collector

The bulk electronic structure of kaolinite (001) plane was studied with quantum mechanical calculations. The CASTEP parameterization of ultrasoft pseudopotentials without core corrections was used to optimize the structure of kaolinite bulk and slab models. The results show that Fermi energy of kaolinite (001) plane is 3.05 eV, and the band gap is 4.52 eV. The partial density of states (PDOS) of kaolinite (001) plane indicates that Al—O and Si—O bonds on the mineral surface are highly polar. The oxygen atoms of hydroxyl groups in surface layer are capable of forming hydrogen bond with the head group of cationic collectors. The properties of dodecylamine (DDA) cation were also calculated by density function theory (DFT) method at B3LYP/6-31G (d) level for illuminating the flotation processes of kaolinite. Besides the electrostatic attraction, the mechanism between kaolinite and DDA is found to be hydrogen bonds under acidic condition. Foundation item: Project(2005CB623701) supported by the Major State Basic Research and Development Program of China; Project(50874118) supported by the National Nature Science Foundation of China; Project(2007B52) supported by the Foundation for the Author of National Excellent Doctoral Dissertation of China     

Advanced sludge reduction and phosphorous removal process

An advanced sludge reduction process, i.e. sludge reduction and phosphorous removal process, was developed. The results show that excellent sludge reduction and biological phosphorous removal can be achieved perfectly in this system. When chemical oxygen demand ρ（COD） is 332 - 420 mg/L, concentration of ammonia p（NH3-N） is 30 - 40 mg/L and concentration of total phosphorous p（TP） is 6.0 -9.0 mg/L in influent, the system still ensures ρ（COD）〈23 mg/L, ρ（NH3-N）〈3.2 mg/L and ρ（TP）〈0. 72 mg/L in effluent. Besides, when the concentration of dissolved oxygen ρ（DO） is around 1.0 mg/L, sludge production is less than 0. 140 g with the consumption of 1 g COD, and the phosphorous removal exceeds 91 %. Also, 48.4% of total nitrogen is removed by simultaneous nitrification and denitrification.     

Adsorption characteristics of thiobacillus ferrooxidans on surface of sulfide minerals

By using thiobacillus ferroxidans (T. f) from Qixiashan, Hubei Province, China, the adsorption characteristics of T. f on surface of sulfide mineral were studied. The influences of adsorption time, pH value, temperature, initial inoculated concentration of bacteria, concentration of sulfide mineral powder, and variety of minerals on the adsorption characteristics were firstly investigated by using the ninhydrin colorimetric method, and the changes of contact angles and Zeta potentials of mineral surface during the bacterial adsorption were then determined. The results show that when the leaching experiments are performed for a long time from several days to a month, the maximal quantity of adsorption of T f on the surface of pyrite is obtained under the following conditions: leaching for 20 d, pH value in range of 1–2 and temperature at 30 °C, respectively; when the bio-leaching experiments are performed for a shorter leaching time, the maximal quantity of adsorption is obtained under the conditions: bio-leaching for 2 h, at 2.4×10⁷ cell/mL of initial inoculated bacteria concentration, and at 10% of mineral powder concentration; and the adsorption quantities are different from one sulfide mineral to another, and the adsorption of T. f on the surface of sulfide minerals includes three phases: increasing phase, stationary phase and decreasing phase. Foundation item: Project(50321402) supported by the National Natural Science Foundation of China; project (2004CB619204) supported by the National Key Fundamental Research and Development Program of China     

Modeling and simulation of crushing process of spiral mining head

1 INTRODUCTIONThe research of mining deep seabed cobalt-richcrust is a hotspot of international oceanology[1].Based on the mining manganese nodule ,lots of re-searches on mining cobalt crust have been madedeeply and many kinds of methods ,such as thei m-proved CLB, mining vehicle +lifting system +surface supporting ship have been put forward.Mining vehicle consists of collecting machine ,tracked treading machine and spiral mininghead[2 ,3].The first step of mining cobalt crust is tocrush…     

Design and analysis of two-layer anonymous communication system

A new architecture for scalable anonymous communication system（SACS） was proposed. The users were divided into several subgroups managed by different sub-blenders, and all sub-blenders were managed by the main-blender using two layers management scheme. The identity information of members are distributed on different sub-blenders, which makes each member keep much less information and network overload greatly reduce. The anonymity and the overhead of the new scheme were analyzed and compared with that of Crowds, which shows the cost of storage and network overhead for the new scheme largely decreases while the anonymity is little degraded. The experiment results also show that the new system architecture is well scalable. The ratio of management cost of SACS to that of Crowds is about 1：25 while the value of P（1｜H1＋） only increases by 0.001-0.020, which shows that SACS keeps almost the same anonymity with Crowds.     

Microstructure and wear resistance of electro-thermal explosion sprayed stellite coating used for remanufacturing

Electro-thermal explosion directional spraying was used to prepare the stellite coating on substrate of the AISI 1045 steel. The morphologies of cross-section and worn scar, porosity, distribution of elements, micro-hardness and wear resistance of the coating were determined by means of SEM, EDAX, micro-hardness tester and sliding wear tester. Because of the compact construction, good bonding and high hardness, the coating is characterized by good wear resistance. The results show that the mainly failure mode of the stellite coating is micro-plowing. Foundation item: Project(2003AA331130) supported by Hi-tech Research and Development Program of China; Project(50235030) supported by the National Natural Science Foundation of China     

Bi-level programming model for reconstruction of urban branch road network

Considering the decision-making variables of the capacities of branch roads and the optimization targets of lowering the saturation of arterial roads and the reconstruction expense of branch roads, the bi-level programming model for reconstructing the branch roads was set up. The upper level model was for determining the enlarged capacities of the branch roads, and the lower level model was for calculating the flows of road sections via the user equilibrium traffic assignment method. The genetic algorithm for solving the bi-level model was designed to obtain the reconstruction capacities of the branch roads. The results show that by the bi-level model and its algorithm, the optimum scheme of urban branch roads reconstruction can be gained, which reduces the saturation of arterial roads apparently, and alleviates traffic congestion. In the data analysis the arterial saturation decreases from 1.100 to 0.996, which verifies the micro-circulation transportation’s function of urban branch road network. Foundation item: Project(2006CB705507) supported by the National Basic Research and Development Program of China; Project(20060533036) supported by the Specialized Research Foundation for the Doctoral Program of Higher Education of China     

Key technologies in whole lifecycle of electromechanical products: state of art

Nowadays, issues on population, environment and resource have become the world’s attentive focus. Sustainable development has been widely accepted around the world. As one of the biggest waste generator and resources consumer, manufacturing industry is facing more challenges as well as opportunities. In order to reduce both resource consuming and waste discharging effectively, traditionally manufacturing mode should be improved. Newly emerged creative technologies and strategies, including green design, green manufacturing and cleaner production, are regarded as the radical resolvent for current environment and resource issues and they will be the only way in the future that manufacturing will evolve. In this paper, a serial of research on green design/manufacturing and cleaner product theoretically and practically was presented. These creative technology and strategy will give strong support to ‘green’ the whole electromechanical designing and manufacturing processes. Foundation item: Project (2002AA421240, 2004AA420120) supported by the National High Technology Research and Development Program of China; project (50445003) the supported by National Natural Science Foundation of China     

Heating and melting mechanism of stainless steelmaking dust pellet in liquid slag

The heating and melting mechanisms of the pellets immersed in liquid slag were investigated, and the effect of the pellet heating and the melting conditions were studied. The results show that the dust component in the pellet is melted from the surface and no metallic elements are melted before the dust component, the time for the pellet completely melted is reduced as the iron powder content increases since the metallic iron has high thermal conductivity. These are four stages of heating and melting of pellet in liquid slag, they are the growth and melt of solid slag shell, penetration of liquid slag, dissolving of dust component and melting of reduced metals. The lifetime of the solid slag shell is in the range of 7-16 s and increasing the pre-heating temperature of the pellet and the slag temperature can shorten the slag shell lifetime. The time for the dust component in the pellet to be melted completely is in the range of 20-45 s and increasing the pre-heating temperature, especially in the range of 600-800 ℃, can obviously reduce the melting time. A higher slag temperature can also improve the pellet melting and the melting time is reduced by 10-15 s when the slag temperature is increased from 1 450 to 1 550 ℃. The pellet with higher content of iron powder is beneficial to the melting by improving the heat conductivity.     

Digital characterization and mathematic model of sodium penetration into cathode material for aluminum electrolysis

The sodium expansion curves of semi-graphitic cathode and TiB₂/C composite cathode with different TiB₂ contents were measured with the improved Rapoport-Samoilenko apparatus. The mathematic model of the sodium expansion was deduced on the basis of the experimental results. The sodium expansion parameter (a) and penetration rate factor (Q), were introduced into the model. The model was validated with the experimental sodium expansion curves self-measured and reported. The results show that the variation tendency of the sodium expansion parameter (a) and penetration rate factor (Q) is consistent with that of the experimental curves. The model is capable of not only conveniently judging the cathode quality, but also favorably establishing a unified standard of the resistance to sodium penetration of cathode. Foundation item: Project(2005CB623703) supported by the Major State Basic Research and Development Program of China; Project(2008AA030502) supported by the National High-Tech Research and Development Program of China; Project(GUIKEJI0639032) supported by the Science Fund Project of Guangxi Province, China     

Control-oriented dynamic fuzzy model and predictive control for proton exchange membrane fuel cell stack

Proton exchange membrane fuel cell （PEMFC） stack temperature and cathode stoichiometric oxygen are very important control parameters. The performance and lifespan of PEMFC stack are greatly dependent on the parameters. So, in order to improve the performance index, tight control of two parameters within a given range and reducing their fluctuation are indispensable, However, control-oriented models and control strategies are very weak junctures in the PEMFC development. A predictive control algorithm was presented based on their model established by input-output data and operating experiences. It adjusts the operating temperature to 80℃. At the same time, the optimized region of stoichiometric oxygen is kept between 1.8-2.2. Furthermore, the control algorithm adjusts the variants quickly to the destination value and makes the fluctuation of the variants the least. According to the test results, compared with traditional fuzzy and PID controllers, the designed controller shows much better performance.