漳州圣女果皮渣水不溶性膳食纤维提取工艺优化及理化性质测定

以漳州龙海圣女果为原料,利用酶法提取皮渣中的水不溶性膳食纤维(insoluble dietary fiber,IDF)。通过考察料液比,糖化酶的用量、温度、时间与蛋白酶的用量、温度、时间七个单因素对IDF得率的影响,以及进一步经响应面优化实验,得到最优提取工艺为:料液比1∶20 g/mL、糖化酶酶解温度65℃、酶解时间45 min、蛋白酶酶解时间60 min,此条件下IDF得率达59.4%。此外,测得所提IDF的持水力为8.62 g/g,持油力为2.43 g/g,膨胀力为5.06 mL/g;番茄红素含量为7.12 mg/100g;且在10～90μg/mL范围内IDF具有良好的羟自由基清除效果。     

酶法提取鸡蛋壳膜中透明质酸的研究

对酶法提取鸡蛋壳膜中透明质酸的工艺进行了研究,通过单因素试验和正交试验考察了酶解温度、pH、酶用量、料液比和酶解时间对透明质酸提取量影响,确定了胰蛋白酶提取透明质酸的最佳工艺条件为温度50℃,pH为8.5,酶用量为8 000 U,料液比1∶40,提取时间7 h.在此条件下,蛋壳膜中透明质酸的提取量为13.294 mg/g.     

茶叶非水溶性膳食纤维的提取及其理化特性研究

对酶法提物茶叶非水溶性膳食纤维的工艺及其理化性能进行研究,探讨料液比、pH值、酶添加量、酶解温度及酶解时间对茶叶非水溶性膳食纤维(IDF)提取的影响,在单因素试验基础上,通过L16(45)正交试验确定最佳提取条件。结果表明:pH值对茶叶IDF的提取影响最大,其次依次为酶解时间、酶添加量、酶解温度、料液比;其最佳提取工艺为:酶解温度65℃、酶添加量0.004g/g、pH4.0、酶解时间2h、料液比1:10(g/mL),提取率达94.12%;所获茶叶IDF具有良好的持水力、膨胀力、结合水力、持油力、阳离子交换能力及对胆固醇、胆酸钠的吸附能力等特性,是一种优质膳食纤维源。     

水酶法从巴旦木中提油及水解蛋白研究

采用水酶法从巴旦木中同时提取油与水解蛋白。依次使用复合细胞壁多糖水解酶(纤素酶;果胶酶=1:2)和碱性蛋白酶水解巴旦木浆,并对酶解工艺条件进行优化。通过单因素试验及正交试验,确定水酶法提取巴旦木油的最佳工艺条件为料液比1:5、粒径40目、复合细胞壁多糖水解酶用量3.5%、酶解温度40℃、酶解时间4h、碱提pH9.0、蛋白酶用量1.5%、酶解温度50℃、酶解时间2h;水酶法提取巴旦木水解蛋白的最佳工艺为料液比1:5、粒径30目、细胞多糖水解酶用量3%、提取温度50℃、提取时间3h、碱提pH8.5、蛋白酶用量1.5%、酶解温度50℃、酶解时间2.5h。在此最佳条件下进行实验验证,总的巴旦木游离油和水解蛋白得率分别为68.74%和74.39%。     

米糠蛋白提取工艺条件的优化

以米糠为原料,分别采用碱法和酶法对米糠蛋白进行提取。在提取过程中,以蛋白提取率为指标,确定料液比、酶用量、时间和温度对米糠蛋白提取率的影响,通过正交试验确定最佳提取工艺。结果表明：碱法最佳提取工艺为料液比1:10(g/mL)、提取时间2.5h、提取温度45℃,米糠蛋白提取率可达57.8%。酶法最佳提取工艺为酶用量2%(E/S)、酶解时间2.5h、酶解温度50℃,米糠蛋白提取率可达38.4%。     

紫薯不溶性膳食纤维超声辅助酶法提取工艺及抗氧化活性研究

研究超声波辅助酶法提取紫薯不溶性膳食纤维(IDF)的工艺,并对其体外抗氧化性进行评定。考察料液比、超声时间、超声功率、α-淀粉酶用量和酶解温度5个因素对紫薯IDF提取率的影响,在单因素试验的基础上进行正交优化试验,以羟自由基(·OH)体外清除率评价IDF的抗氧化水平。结果表明,紫薯IDF的最佳工艺参数为料液比115(g/mL)、超声时间40 min、超声功率160 W和酶解温度75℃,该条件下IDF的提取率可高达38.32%。体外抗氧化结果显示紫薯IDF对·OH具有良好的清除效果,并且呈现出一定的量效关系。经计算,其IC_(50)值为39.27 mg/mL,抗氧化性强于VC。     

响应面优化复合植物水解酶提取梅州金柚皮可溶性膳食纤维

采用复合植物水解酶结合酸法提取梅州金柚皮中可溶性膳食纤维,以单因素试验为基础,以可溶性膳食纤维提取率为指标,选择酸法适合提取条件,再根据Box-Behnken实验设计原理,通过响应面分析得到酶法提取优化组合条件。得到最佳工艺为:稀硫酸p H3、物料比固定为1:20、酸解温度为50℃,酸解时间2 h,酶解温度为44℃,酶添加量为0.25 mg/g,酶解时间为2 h,此时可溶性膳食纤维提取得率为20.2438 mg/g。膳食纤维的持水力为9.397 g/g,溶胀性为11.25 m L/g,梅州金柚皮可以作为一种优良的膳食纤维来源。     

武夷岩茶茶渣非水溶性膳食纤维提取工艺研究

以冲泡后的岩茶茶渣为原料,采用酶法研究膳食纤维(IDF)的提取工艺参数,得出用α-淀粉酶处理茶粉的粗膳食纤维提取条件是:酶解温度55℃、酶添加量0.06 g/g、p H 9、酶解时间1 h、料液比1∶15(g/m L);木瓜蛋白酶精制IDF的最佳工艺条件是:酶解温度45℃、酶添加量0.004 g/g、p H值为4、酶解时间2 h、料液比1∶15(g/m L),此条件下膳食纤维的提取率最高,为97.12%。     

联合酶法提取豆渣蛋白肽和可溶性膳食纤维

豆渣是大豆加工的主要副产物之一,含有丰富的蛋白质和膳食纤维。为促进豆渣高值化利用,采用联合酶法从豆渣中提取蛋白肽和可溶性膳食纤维（SDF）。首先用碱性蛋白酶酶解豆渣蛋白,以蛋白肽得率为指标,通过单因素试验优化了提取豆渣蛋白肽的工艺条件,再将脱蛋白豆渣用纤维素酶酶解制备SDF,以SDF提取率为指标,通过单因素试验优化提取SDF的工艺条件。结果表明：碱性蛋白酶酶解提取蛋白肽最佳工艺条件为料液比1∶ 35、酶与底物比2%、酶解时间5 h、酶解温度50 ℃、pH 95,在此条件下豆渣蛋白肽得率为66.81%;纤维素酶酶解提取SDF最佳工艺条件为料液比1∶ 30、酶与底物比3%、酶解温度50 ℃、酶解时间2 h、pH 4.0,在此条件下SDF提取率为1554%。利用碱性蛋白酶和纤维素酶依次酶解后,豆渣总利用率达到了89.81%,这为豆渣综合开发利用提供了一种新途径。     

榕树果总黄酮酶法辅助回流提取研究

对纤维素酶法提取榕树果总黄酮的工艺进行研究。在单因素试验的基础上,采用L9(34)正交试验法对酶法提取榕树果总黄酮的条件进行优化,考察酶用量、酶解温度、p H、酶解时间对榕树果总黄酮提取率的影响。结果表明:最佳提取工艺条件为酶用量25%,酶解时间30 min,酶解温度50℃,酶解p H5.5。在该条件下测定榕树果总黄酮的提取率为10.22 mg/g。     

Reliable identification of grapevine rootstock varieties using RAPD PCR on woody samples

Since grapevine ( Vitis spp .) rootstock material is being traded increasingly as disbudded woody material a lack of distinctive morphological features on such material necessitates an alternative and reliable means of identification. Methods described here were developed for rapid and efficient extraction of DNA from woody samples rich in phenolic compounds and polysaccharides, and for subsequent identification of varieties by RAPD PCR. Using these methods, and with the application of only one selected RAPD primer, we were able to differentiate sixteen rootstock varieties, including the seven varieties most commonly used in Germany. Problems commonly encountered with reproducibility of RAPD patterns were avoided by choosing primers with a dinucleotide sequence and a high G/C content that allowed a rather high annealing temperature of 45°C. Methods described here should also be useful for other horticultural crops, especially those with woody tissues rich in phenolic compounds and polysaccharides.     

An internet compendium of analytical methods and spectroscopic information for monomers and additives used in food packaging plastics

An internet website (http://cpf.jrc.it/smt/) has been produced as a means of dissemination of methods of analysis and supporting spectroscopic information on monomers and additives used for food contact materials (principally packaging). The site which is aimed primarily at assisting food control laboratories in the European Union contains analytical information on monomers, starting substances and additives used in the manufacture of plastics materials. A searchable index is provided giving PM and CAS numbers for each of 255 substances. For each substance a data sheet gives regulatory information, chemical structures, physico-chemical information and background information on the use of the substance in particular plastics, and the food packaging applications. For monomers and starting substances (155 compounds) the infra-red and mass spectra are provided, and for additives (100 compounds); additionally proton NMR are available for about 50% of the entries. Where analytical methods have been developed for determining these substances as residual amounts in plastics or as trace amounts in food simulants these methods are also on the website. All information is provided in portable document file (PDF) format which means that high quality copies can be readily printed, using freely available Adobe Acrobat Reader software. The website will in future be maintained and up-dated by the European Commission's Joint Research Centre (JRC) as new substances are authorized for use by the European Commission (DG-ENTR formerly DGIII). Where analytical laboratories (food control or other) require reference substances these can be obtained free-ofcharge from a reference collection housed at the JRC and maintained in conjunction with this website compendium.     

Aroma characterization of various apricot varieties using headspace–solid phase microextraction combined with gas chromatography–mass spectrometry and gas chromatography–olfactometry

The characterization of the aromatic profile of several apricot cultivars with molecular tracers in order to obtain objective data concerning the aromatic quality of this fruit was undertaken using headspace–solid phase microextraction (HS–SPME). Six apricot cultivars were selected according to their organoleptic characteristics: Iranien, Orangered, Goldrich, Hargrand, Rouge du Roussillon and A4025. The aromatic intensity of these varieties measured by HS–SPME–Olfactometry were defined and classified according to the presence and the intensity of grassy, fruity and apricot like notes. In the six varieties, 23 common volatile compounds were identified by HS–SPME–GC–MS. Finally, 10 compounds, ethyl acetate, hexyl acetate, limonene, β-cyclocitral, γ-decalactone, 6-methyl-5-hepten-2-one, linalool, β-ionone, menthone and (E)-hexen-2-al were recognized by HS–SPME–GC–O as responsible of the aromatic notes involved in apricot aroma and considered as molecular tracers of apricot aromatic quality which could be utilized to discriminate apricot varieties.     

Tests of potential functional barriers for laminated multilayer food packages. Part I: Low molecular weight permeants

The advent of the functional barrier concept in food packaging has brought with it a requirement for fast tests of permeation through potential barrier materials. In such tests it would be convenient for both foodstuffs and materials below the functional barrier (sub-barrier materials) to be represented by standard simulants. By means of inverse gas chromatography, liquid paraffin spiked with appropriate permeants was considered as a potential simulant of sub-barrier materials based on polypropylene (PP) or similar polyolefins. Experiments were performed to characterize the kinetics of the permeation of low molecular weight model permeants (octene, toluene and isopropanol) from liquid paraffin, through a surrogate potential functional barrier (25 μm-thick oriented PP) into the food simulants olive oil and 3% (w/v) acetic acid. These permeation results were interpreted in terms of three permeation kinetic models regarding the solubility of a particular model permeant in the post-barrier medium (i.e. the food simulant). The results obtained justify the development and evaluation of liquid sub-barrier simulants that would allow flexible yet rigorous testing of new laminated multilayer packaging materials.     

Analysis of benzo[a]pyrene in spiked fatty foods by second derivative synchronous spectrofluorimetry after microwave-assisted treatment of samples

A simple, rapid and inexpensive method has been developed for the determination of benzo[a     

The development of reference materials for paralytic shellfish poisoning toxins in lyophilized mussel. II: Certification study

This paper describes the second part of a project undertaken to develop certified mussel reference materials for paralytic shellfish poisoning toxins. In the first part two interlaboratory studies were undertaken to investigate the performance of the analytical methodology for several PSP toxins, in particular saxitoxin and decarbamoyl-saxitoxin in lyophilized mussels, and to set criteria for the acceptance of results to be applied during the certification exercise. Fifteen laboratories participated in this certification study and were asked to measure saxitoxin and decarbamoyl-saxitoxin in rehydrated lyophilized mussel material and in a saxitoxin-enriched mussel material. The participants were allowed to use a method of their choice but with an extraction procedure to be strictly followed. The study included extra experiments to verify the detection limits for both saxitoxin and decarbamoyl-saxitoxin. Most participants (13 of 15) were able to meet all the criteria set for the certification study. Results for saxitoxin.2HCl yielded a certified mass fraction of <0.07 mg/kg in the rehydrated lyophilized mussels. Results obtained for decarbamoyl-saxitoxin.2HCl yielded a certified mass fraction of 1.59+/-0.20 mg/kg. The results for saxitoxin.2HCl in enriched blank mussel yielded a certified mass fraction of 0.48 +/- 0.06 mg/kg. These certified reference materials for paralytic shellfish poisoning toxins in lyophilized mussel material are the first available for laboratories to test their method for accuracy and performance.     

Announcing a new international peer-reviewed journal:Food Science and Human Wellness now accepting manuscript submission in English

<正>We are pleased to announce the launch of a new international peer-reviewed journal-Food Science and Human Wellness,ISSN 2213-4530,which is an open access journal,produced and hosted by Elsevier B.V.on behalf of Beijing Academy of Food Sciences.Food Science and Human Wellness is an international peer-reviewed English journal that provides a forum for the dissemination of the     

Chinese CTP Market

According to Printing and Printing Equipment Industries Association of China（PEIAC）＇s statistics to the plate manufucturer in China, in 2013, the actual offset plate production has reached 346 million square meters in China. Among them, the CTP production volume was 245 million square meters, up by 11% than that of last year; the total sales of the CTP plate was 239 million square meters, up by 13%.     

Preparatory Work of Print China 2015 is Going Smoothly

正Held at Guangdong Modern International Exhibition Center,Print China 2015 will cover 7exhibition halls,besides the original Hall No.3,4,5,6,7,the newly built F zone of Hall 3 will be used too.The total area will be140,000 square meters.Hall 3:Offset and large printing equipment,package printing equipment,post press