共查询到19条相似文献,搜索用时 500 毫秒
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以井冈霉素生产菌株7105#为出发菌株,采用氮离子注入诱变处理,经摇瓶筛选,得到一株特性优良且A组分含量较高的9263#菌株。 相似文献
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为提高棘白霉素B脱酰基酶产生菌的转化率,通过紫外诱变复合Cu2+抗性处理筛选转化高产菌种,利用均匀设计方法优化菌种的转化工艺。结果表明,筛选得到的突变株132#的棘白霉素B转化率比出发菌株提高了22.6%,优化后的转化条件为:底物浓度2 400μg·mL-1,转化时间56h,转化温度30℃,发酵培养基pH值6.0。132#菌株在优化转化条件下的转化率较出发菌株在原始转化条件下提高了57.3%。优化的转化条件更有利于高产菌株的转化,转化率得到大幅提高,也为其工业化应用打下基础。 相似文献
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探讨了自行培养的2#、4#、9#菌在降解芘过程中的相互作用关系。分别对单一菌株、菌株间两两混合、三株菌混合三种情形对芘的降解作用进行研究。研究结果表明:三株菌中2#菌株的降解效果最好,5d内累积去除率为55.99%;菌株间两两混合时2#菌株的降解受到抑制,说明菌株间有相互抑制的作用;三株菌混合对芘降解时同样表现出相互抑制作用,抑制程度大于两两混合的情况,在降解过程中,pH值总体是下降的,并周期性地交替出现产酸和脱羧反应。 相似文献
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通过细菌的初筛和复筛,得到了硫化钠脱硫效果较好的一株菌株命名为S1#。通过对菌株S1#培养条件的测定,得到菌株的最适培养条件:pH值,8;温度,45℃;摇床转速,200r/min;代谢负荷,(Na2S)3~5g/L,通过实验发现该菌株为兼氧菌株,能在氧气不足的环境下良好生长。在最适培养条件下对菌株S1#进行了生长曲线的测定,实验表明在S1#在12h进入对数生长期,菌株S1#的浊度值在30h的时候达到了最高峰。通过对菌株脱硫效果的测定,菌液中的硫化钠能很好地降低,将细菌对硫化钠脱硫效果测定时的溶液中沉淀烘干XRD衍射检测,发现有单质硫的存在,说明细菌能代谢硫化钠,并得到了单质硫。 相似文献
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Yugo Iwasaki Wanda Brown Ching T. Hou 《Journal of the American Oil Chemists' Society》2002,79(4):369-372
The biosynthetic pathway of two bicyclic FA, 12∶17, 13∶17-diepoxy-9(Z)-octadecenoic acid (DEOA) and 7-hydroxy-12∶17, 13∶17-diepoxy-9(Z)-octadecenoic acid (hDEOA), by Clavibacter sp. ALA2 was investigated. When cultivated with linoleic acid as a substrate, the strain produced 12,13,17-trihydroxy-9(Z)-octadecenoic acid (THOA), DEOA, and hDEOA as well as other FA. To clarify the synthetic route to these bicyclic FA, the
strain was cultivated with purified THOA as a starting substrate. THOA was consumed almost completely by the strain with sequential
generation of DEOA and hDEOA. Moreover, the strain produced hDEOA when cultivated with purified DEOA. Therefore, it was confirmed
that THOA was a precursor of these bicyclic FA and that hDEOA was generated from DEOA. Based on our previously reported result
that linoleic acid is first converted to 12,13-dihydroxy-9(Z)-octadecenoic acid (DHOA) and the present results, the overall biosynthetic pathway for the diepoxy bicyclic FA from linoleic
acid was postulated as: linoleic acid→DHOA→THOA→DEOA→hDEOA. 相似文献
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Y. S. Zhao Y. Yang J. T. Li I. P. Borovinskaya K. L. Smirnov 《International Journal of Self-Propagating High-Temperature Synthesis》2009,18(2):87-91
Silicon was used as the only combustible agent, and single-phase Y-α-SiAlON powders were fabricated from a proper starting
composition, but the grain morphology of the products was represented by equiaxed microcrystals. For the same starting materials,
as a modified method of combustion synthesis was applied, single-phase Y-α-SiAlON powders were also obtained and the grain
morphology of the products changed to rod-like microcrystals. It is of great importance to control the reaction temperature
to tailor the grain morphology of Y-α-SiAlON microcrystals.
The article is published in the original. 相似文献
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磷细菌突变株生理特性的研究 总被引:6,自引:0,他引:6
本文对经过筛选培育出的一株解磷能力较强的磷细菌突变株D017P的生理特性进行了研究。其中,最佳氮源为麦麸汁,与出发菌株所用马铃薯汁相比,生物量增加9.8倍,解磷能力提高71.4%,最佳碳源为蔗糖,与出发菌株所用碳源葡萄糖比较,解磷能力提高28.8%,金属离子,培养温度,pH,气/液比等也对突变株的生长有一定影响,其结果可作为选定工业化条件的参考。 相似文献
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Horlick R.A.; George H.J.; Cooke G.M.; Tritch R.J.; Newton R.C.; Dwivedi A.; Lischwe M.; Salemme F.R.; Weber P.C.; Horuk R. 《Protein engineering, design & selection : PEDS》1992,5(5):427-431
A technique for the rapid and simple generation of permutatedversions of the interleukin-1ß (IL-1ß) geneis described. In this method, the human IL-1ß cDNAis twice amplified by the polymerase chain reaction (PCR) andthe resulting DNA fragments are ligated in tandem. Between thetwo genes, the DNA sequence encodes a short four amino acidloop to link the native N- and C-terminal ends of the IL-1ßprotein. By using PCR amplification from this starting template,a new version of the IL-1ß cDNA was obtained thatencodes a permutated form of the IL-1ß protein wherethe new N- and C-terminal amino acids correspond to residues65 and 64 of the native IL-1ß sequence, respectively.The name permutein is proposed to describe proteinsgenerated by this technology. The molecular profile (IL-1 receptorbinding, biologic activity and solution properties) of the IL-1permutein produced by this technology, permutein 65/64, is shownto be identical to that of native IL-1ß The approachshould be useful to define further the structural features ofthis protein that are important for its function. 相似文献
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A method of combined approximation of isothermal “families” of creep and relaxation curves is proposed for obtaining more
precise numerical values of the viscoelastic characteristics of cloth. The applicability of the equation of nonlinear-hereditary
elasticity designed for analytical prediction of the stress—strain state of the fabric and in particular, stress—strain diagrams
and more complex strain regimes was demonstrated.
St. Petersburg State University of Technology and Design. Translated fromKhimicheskie Volokna, No. 1, pp. 40–43, January–February, 1999. 相似文献