亨氏马尾藻醇提取物及其不同溶剂萃取物的抗氧化活性研究

研究了亨氏马尾藻95%乙醇提取物不同溶剂级分的体外抗氧化活性。采用Folin-Ciocalteu法测定亨氏马尾藻醇提取物（总提取物）以及石油醚萃取物、乙酸乙酯萃取物、正丁醇萃取物和水萃取物五个部位的总酚含量,并通过DPPH·、ABTS⁺·清除及ORAC三种方法评价五种提取物的抗氧化活性。结果表明,乙酸乙酯萃取物的总酚含量最高,为（12.71±0.02）mg GAE/g（没食子酸当量）。在不同抗氧化测定方法中,亨氏马尾藻醇提取物及其不同溶剂萃取物均表现出不同程度的抗氧化活性。其中,乙酸乙酯萃取物抗氧化活性较强,其DPPH和ABTS半数清除率分别为（0.89±0.01）mg/m L和（0.73±0.03）mg/m L,ORAC值达到（1803.78±81.37）μmol TE/g。      

蓝靛果乙醇洗脱物的抗氧化活性研究

蓝靛果花色苷是一种很好的抗氧化剂,其花色苷种类繁多,为筛选出具有较高抗氧化活性的花色苷,将蓝靛果花色苷粗提物经X-5大孔树脂吸附后,用体积分数10%、20%、30%、40%、50%、60%、70%、80%乙醇溶液梯度洗脱获得8个洗脱物,研究了这8个洗脱物对.OH、O2-.、DPPH.和ABTS+.的清除作用。8个洗脱物均表现出较强的清除能力,并且清除率随各物质量浓度的升高而提高,而且清除率大都高于VC对照组,其中,60%乙醇洗脱物清除.OH和O2-.能力最强,40%乙醇洗脱物清除DPPH.能力最强,10%乙醇洗脱物清除ABTS+.能力最强。实验结果表明,蓝靛果提取物的乙醇洗脱物具有很强的清除自由基的能力,可以作为抗氧化剂应用在食品、化妆品、药品等领域。     

蓝莓叶不同溶剂提取物抗氧化活性研究

研究蓝莓叶不同溶剂提取物中总酚和黄酮的含量以及抗氧化活性。分别用水、甲醇、乙醇、乙酸乙酯、氯仿、石油醚等不同极性溶剂提取蓝莓叶中的活性物质,采用Folin-Cioeaile法、三氯化铝显色法分别测定不同提取物中总酚和黄酮的含量;应用1,1-二苯基-2-三硝基苯肼（DPPH）自由基和2,2-联氮基双（3-乙基苯并噻唑啉-6-磺酸）二铵盐（ABTS）自由基清除体系及β-胡萝卜素-亚油酸体系评价其抗氧化活性,考察活性物质含量与抗氧化活性的关系。结果表明,蓝莓叶提取物的抗氧化活性随提取溶剂极性的减小而减小。水提取物具有较强的抗氧化活性,其清除DPPH·和ABTS+·的EC50分别为（12.78±0.10）、（6.35±0.03）μg/mL,抑制β-胡萝卜素淬灭IC50值为（25.33±0.02）μg/mL。含量测定显示水提物中总酚含量最高,为（264.67±0.29）mg/g,而甲醇提取物中黄酮含量最高,为（68.94±0.08）mg/g,相关性分析表明,不同溶剂提取物抗氧化能力差别较大,总酚和黄酮含量与抗氧化能力具有相关性,提示水可作为蓝莓叶抗氧化活性物质提取的优选溶剂,而总酚和黄酮含量可作为蓝莓叶抗氧化提取物的质量评价指标。      

Antioxidative and anti-inflammatory activities of an ethanol extract from fig (Ficus carica) branches

The edible fruit of common fig (Ficus carica) or just the fig has been traditionally used for treating hemorrhoids, insect stings, gout, ulcers, and skin infections such as warts and viruses. An ethanol extract of fig branches and its ethyl acetate, hexane, butanol, and water fractions were prepared and examined for their abilities to scavenge free radicals and inhibit inflammatory reactions. Our data showed that the ethyl acetate fraction contained the largest amount of phenolic compounds and showed the highest free radical scavenging activity. Every fraction of fig, particularly the ethanol extract and the ethyl acetate and hexane fractions, inhibited nitric oxide production in RAW264.7 cells. Tumor necrosis factor-α level also decreased significantly in all groups tested. Our results demonstrate that fig branches possessed pharmacological activity and might be useful for developing antioxidant or antiinflammatory agents.     

Antioxidant and cytotoxic activities of various fractions of ethanol extract of Dianthus superbus

This study examined the antioxidant and cytotoxic activities of various fractions (prepared by using solvents of varying polarity) of ethanol extract of Dianthus superbus (DS). The antioxidant activities of various fractions were evaluated in terms of their free radical-scavenging activity. The total phenolics and reducing powers of the fractions were measured. The cytotoxic activities of the fractions on three human malignant cell lines were also investigated. Among all fractions, the ethyl acetate fraction (EE-DS), which had the highest phenolic content and the strongest reducing power, exhibited the strongest antioxidant and cytotoxic activities.     

蓝靛果花色苷乙醇洗脱物抗癌活性的研究

蓝靛果花色苷具有较好的抗癌功能,其花色苷种类繁多,为筛选出具有较高抗癌作用的花色苷,将蓝靛果花色苷粗提物经X-5大孔树脂吸附后,用体积分数10%、20%、30%、40%、50%和60%乙醇溶液梯度洗脱获得6种洗脱物,研究了这6种洗脱物对人肺癌细胞株A-549,人肝癌细胞株HepG2和人宫颈癌细胞株Hela的细胞抑制率并进行了比较。6种洗脱物对三种癌细胞都具有一定的抑制率,其中抑制率最强的是10%乙醇洗脱物,且对三种癌细胞抑制率从大到小顺序为：Hela〉HepG2〉A-549。实验结果表明,蓝靛果提取物的乙醇洗脱物具有很强的抑制癌细胞的能力,可以用来预防癌症,应用在医疗、食品、药品等领域。     

Antioxidant and antigenotoxic activities of ethanol extracts from Rhus chinensis Mill leaves

Ethanol extracts were obtained from Rhus chinensis Mill (RCM) leaves and used for antioxidant and antigenotoxic activity assays. IC₅₀ values in DPPH assays were 15.96, 18.83, 20.43, 27.93, 37.43, 46.21, and 141.84 μg/mL for TPP, IPE, LLE, Vc, CE, BHT, and Trolox. Similar results were obtained using ABTS and FRAP assays. In vivo testing showed strong antioxidant activities that were positively correlated with polyphenol contents. Leaf tissue contained abundant polyphenols, and more than 10 phenolic compounds were detected in extracts. Quantitative results showed that quercetin-3-rhamnoside (26.4±0.76 mg/g of extract) was the most abundant ingredient, followed by hyperoside (15.2±0.42 mg/g of extract), quercetin (1.5±0.07mg/g of extract), and kaempferol (0.48±0.05 mg/g of extract). This study increases the knowledge for possible uses of forest by-products as a substitute for gallnuts.     

红毛七乙醇提取物和不同极性部位抗氧化活性的研究

主要从清除DPPH自由基(DPPH.)、超氧阴离子自由基(O2-.)、羟基自由基(.OH)和亚硝酸盐(NO2-)四个方面,研究红毛七乙醇提取物和不同极性萃取部位的体外抗氧化活性。结果表明,红毛七乙醇提取物和不同极性部位对DPPH.、O2-.、.OH、NO2-均具有清除能力,其中乙醇提取物、正丁醇与乙酸乙酯中等极性部位抗氧化活性较强,而极性较小的石油醚部位和极性较大的水部位抗氧化作用较弱。     

金瓜多糖不同分级组分的抗氧化和降血糖活性

目的:研究金瓜多糖不同分级组分的理化特性、结构、抗氧化性和降血糖活性。方法:采用水提醇沉法分级纯化得到4种金瓜多糖(CP-40、CP-60、CP-70、CP-80),通过高效液相色谱、傅里叶变换红外光谱等对其分子量、单糖组成、基团构成、抗氧化和降血糖活性进行研究。结果:金瓜多糖组分随着乙醇体积分数的增加,总糖含量升高,糖醛酸含量降低。4种多糖分子量不同,单糖组成相同但组成比例不同,且均具有多糖类物质的特征吸收峰。金瓜多糖体外抗氧化能力和降血糖活性存在明显的量—效关系,CP-70对DPPH自由基、羟自由基和超氧阴离子自由基清除作用高于其他组,抗氧化性最强,CP-80对α-葡萄糖苷酶和α-淀粉酶抑制作用最强,降血糖活性最佳。结论:金瓜多糖具有良好的抗氧化性和降血糖活性。     

Antioxidant and anti-inflammatory activities of phenolic compounds extracted from lemon myrtle (Backhousia citriodora) leaves at various extraction conditions

Food Science and Biotechnology - Lemon myrtle leaves were extracted with ethanol at different temperatures (25, 50, and 80 °C) and times (2, 4, 6, and 10 h) to examine the...     

Antioxidant activities of different fractions of polysaccharide conjugates from green tea (Camellia Sinensis)

Three fractions of water-soluble polysaccharide conjugates, coded as TPC-1, TPC-2, and TPC-3, were isolated and purified from low-grade green tea (Camellia sinensis) by absorbent chromatography and ion exchange chromatography. Their chemical and physical characteristics were determined by chemical methods, gas chromatography and size exclusion chromatography with laser light scattering. Deoxyribose assay, photoreduction of Nitro Blue Tetrazolium (NBT) assay and lipid peroxidation inhibition assay were applied to test the antioxidant activities of tea polysaccharide conjugates in vitro. The results indicated that the three polysaccharide conjugates were heteropolysaccharides bounded with protein. TPC-1 was composed of l-arabinose (Ara), d-ribose (Rib), d-xylose (Xyl), d-glucose (Glc), d-galactose (Gal) and d-mannose (Man). TPC-2 was only composed of four monosaccharides: Ara, Rib, Glc and Man. There was no Man detected in TPC-3. The protein contents of TPC-1, TPC-2, and TPC-3 were 2.8%, 3.8% and 4.0% and the molecular weights were 26.8 × 10⁴, 11.8 × 10⁴, 4.2 × 10⁴, respectively. TPC-3 showed the highest antioxidant activities among the three fractions of polysaccharide conjugates, with an IC₅₀ of 182 μg/ml for the deoxyribose assay, and 93 μg/ml for the photoreduction of NBT assay, values which were lower than those of TPC-1 and TPC-2 (P < 0.01). The effects of the molecular weight and protein content of the polysaccharide conjugates on the improvement of the bioactivities appeared to be significant.     

人心果叶不同极性组分的抗氧化与抗菌活性

通过体外DPPH自由基、ABTS自由基清除能力和总抗氧化能力评价人心果叶不同极性萃取物(石油醚相、乙酸乙酯相、正丁醇相、水相)的抗氧化活性,并探究各极性组分对金黄色葡萄球菌、枯草芽孢杆菌和大肠杆菌的抑制能力.结果表明:乙酸乙酯组分的体外抗氧化活性最强,清除DPPH自由基能力IC50值为(0.019±0.010)mg/m...     

定心藤醇提物不同极性部分的体外抗氧化活性研究

研究定心藤75%乙醇提取物不同极性部位的体外抗氧化活性,采用5种不同极性溶剂萃取（石油醚、氯仿、乙酸乙酯、正丁醇、水）得到5种萃取物,分别测定黄酮含量,并通过还原能力测定、DPPH自由基清除能力、羟自由基清除能力和超氧阴离子自由基清除能力4种测定方法,对各萃取物抗氧化能力进行研究。结果显示,各提取物对Fe3+均有一定的还原能力,乙酸乙酯萃取物还原效果最为明显;定心藤不同极性部位均具有一定的抗氧化活性,乙酸乙酯萃取物抗氧化能力最强,DPPH·的半数清除浓度IC50为0.28mg/m L,羟自由基半数清除浓度IC50为0.41mg/m L,在0.2mg/m L浓度下,对超氧阴离子自由基的清除率达到42.88%,而其黄酮含量最高,达到609.96mg/g,这也许与它具有较高的抗氧化活性有关。说明乙酸乙酯萃取物具有较好的开发天然抗氧化剂的前景。      

Antioxidant activities of extract and fractions from Tuber indicum Cooke & Massee

The antioxidant activities of ethanolic crude extract (ECE) and its four different solvent sub-fractions (namely, petroleum ether fraction (PEF), ethyl acetate fraction (EAF), n-butyl alcohol fraction (BAF) and the rest fraction (RF)) from Tuber indicum were investigated using several in vitro antioxidant assays. ECE and four sub-fractions possessed different antioxidant and radical-scavenging activities in different assays. BAF showed the most potent radical-scavenging activity on DPPH radicals, superoxide anion radicals and reducing power, with EC₅₀ values of 1.38, 0.96, 16.0 mg/ml, respectively. EAF exhibited the highest hydroxyl radical-scavenging and ferrous ion chelating activities with EC₅₀ values of 3.31 and 0.70 mg/ml, respectively. The total phenolics (TP) and total flavonoids (TF) were also determined. BAF had the highest TP and TF contents, and the next was EAF. These results showed that the amounts of phenolics and flavonoids were in accordance with higher effectiveness in scavenging radicals and chelating ferrous ions.     

豆腐柴叶总黄酮不同溶剂级分抗氧化活性分析

采用1,1-二苯基-2-三硝基苯肼自由基、羟自由基、超氧阴离子、还原力、总抗等体外抗氧化活性模型和抗氧化活性综合评价指数,分析四川江油地区不同溶剂萃取的豆腐柴黄酮级分（乙醇、乙酸乙酯、正丁醇、氯仿、水）的抗氧化活性。结果显示,不同浓度乙醇提取总黄酮的1,1-二苯基-2-三硝基苯肼自由基清除活性最高。1,1-二苯基-2-三硝基苯肼自由基清除率、羟基自由基清除率、ABTS阳离子自由基清除率、总抗和还原力皆随乙醇浓度的升高而呈现先增强后减弱的趋势,超氧阴离子自由基清除率却随乙醇浓度增加一直降低。综合评价指数显示不同浓度乙醇抗氧化活性依次为75%醇提物>65%醇提物>55%醇提物>85%醇提物>95%醇提物。乙酸乙酯层萃取物的1,1-二苯基-2-三硝基苯肼自由基清除率、总抗、还原力及ABTS阳离子自由基清除率要优于其它各萃取层。正丁醇层萃取物的超氧阴离子自由基清除率要优于其它各萃取层。乙酸乙酯层抗氧化活性最佳。豆腐柴叶总黄酮有较好的抗氧化活性,可作为抗氧化剂或者健康食品原料开发。      

Evaluation of Synurus deltoides for antioxidant,antimicrobial, and anti-proliferative activities using In vitro assays

The antioxidant, antimicrobial, and anti-proliferative activities of water and ethanol extracts from leaves of Synurus deltoides (SD) were investigated. Extracts showed strong reductive powers, nitrite-scavenging activities, DPPH, ABTS, hydroxyl, and superoxide radical scavenging activities, and DNA damage prevention activities. SD extracts also showed strong cellular antioxidant activities in a dose-dependent manner with IC₅₀ values of 0.93 and 0.65 mg/mL for water and ethanol extracts, respectively. Antimicrobial activities were evaluated against 4 microorganisms. SD extracts showed high inhibitory activities against Pseudomonas aeruginosa and Staphylococcus aureus. The anti-proliferative activity was dose-dependent against human colon adenocarcinoma (HT-29) and mouse rectum carcinoma cells (CMT-93). In addition, HPLC analyses of SD leaf extracts revealed the presence of different phenolic compounds. SD can be considered as a source of functional foods and pharmaceuticals.     

Antioxidant,anti-inflammatory and acetylcholinesterase inhibitory activities of propolis from different regions of Morocco

Propolis is a bee product used since ancient times in folk medicine. Antioxidant activity is one of the properties attributed to this natural product, which varies depending on the botanical and geographic origin. The antioxidant capacities of propolis from different regions of Morocco were determined. Samples from the Khamissat and Immouzzer regions showed the highest antioxidant activities (ABTS assay), reducing power, and peroxyl and hydroxyl radical scavenging capacities. The best capacities to inhibit 5-lipoxygenase were found in propolis samples from Rabat. A strong correlation was found between the amounts of phenols, flavonoids, and antioxidant and anti-inflammatory activities. These results support the hypothesis that phenols contribute greatly to the pharma-cological properties of propolis and suggest that propolis could be important in prevention of diseases in which free radicals are implicated.     

Antioxidant activities of lotus leaves (Nelumbo nucifera) and barley leaves (Hordeum vulgare) extracts

This study evaluated the antioxidant activities of lotus leaf extracts by methanol (L/M) and ethanol (L/E), and barley leaf extracts, also by methanol (B/M), and ethanol (B/E). The 2,2-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity of L/M was higher than that of the other extracts at 250 ppm, with the exception of butylhydroxytoluene (BHT). The reducing powers of the treatments at 250 ppm were in the following order: BHT (2.91)>L/M (2.90)>L/E (2.26)>B/E (2.09)>B/M (2.01). In addition, L/M showed the highest concentration of total phenols, total flavonoids, and strongest superoxide dismutase (SOD)-like activity among the treatments. Thus, lotus and barley leaves extracted by methanol and ethanol that have antioxidant activities may be alternatives to synthetic antioxidants in the food industry.     

侧柏球果体外抑菌作用初探

目的：观察侧柏球果乙醇提取物的体外抑菌活性。方法：运用渗漉法提取并制备石油醚、乙酸乙酯、正丁醇和水萃取物,用滤纸片法对粗提物及各萃取物进行粗筛,有抑菌作用的萃取物进一步用微量肉汤稀释法测定其最低抑菌浓度（MIC）。结果：侧柏球果乙醇提取物及石油醚和乙酸乙酯萃取物具有抑制金黄色葡萄球菌等3种孳兰氏阳性菌和大肠埃希菌等3种举兰氏阴性菌生长繁殖的活性,乙醇提取物抑菌作用最强,对金黄色葡萄球菌、大肠埃希菌和肺炎克雷伯菌的MIC均为19．6μg·mL-1,对地衣芽孢杆菌、无乳链球菌和铜绿假单胞菌的MIC均为39．1μg·mL-1;石油醚萃取物对6种测试菌的MIC均为78．2ug·mL-1,乙酸乙酯萃取物对铜绿假单胞菌的MIC为625μg·mL,对无乳链球菌和肺炎克雷伯菌的MIC为312．5ug·mL-1,对其余3种测试菌的MIC为156．3ug·mL-1结论：侧柏球果乙醇提取物及石油醚和乙酸乙酯萃取物能湿著抑制金黄色葡萄球菌、地农芽抱杆菌、无乳链球菌、大肠埃希菌、肺炎克雷伯菌和铜绿假单胞菌的生长繁殖。