叶绿素铜钠盐牙膏的抗炎止血效果试验

目的：研究叶绿素铜钠盐牙膏的抗炎止血效果。方法：以二甲苯致小白鼠耳廓急性炎症肿胀为模型，在急性炎症肿胀耳廓局部涂抹受试物，观察样品对急性炎症肿胀的抗炎效果；用小白兔实质器官肝脏局部创面损伤出血的止血时间为评价指标，观察样品的止血效果。结果：与牙膏空白基质组比较，牙膏试验组的抗炎、止血效果均具有显著性差异。结论：叶绿素铜钠盐牙膏具有较好的抗炎止血效果。     

墨旱莲的药理作用及牙膏的止血效果药理试验

介绍了墨旱莲的药理作用。试验在牙膏中添加墨旱莲提取物,通过兔肝脏局部创面损伤出血模型,在出血创面局部,分别敷涂牙膏样品,观察记录样品的止血时间,计算止血效果的分值,比较各试验牙膏的止血效果。试验结果显示：与空白对照组比较,含墨旱莲提取物的牙膏对兔肝脏局部创面损伤出血有明显的止血作用。表明含墨旱莲提取物的牙膏具有止血效果。     

超临界萃取沙棘油的研究

以沙棘果渣和沙棘籽为原料,分别进行沙棘果油和沙棘籽油的CO2超临界萃取的工艺研究,实验表明沙棘果油的最佳萃取条件为：温度40℃,压力30MPa,时间2h,CO2流量25kg／h;沙棘籽油适宜萃取条件为：温度45℃,压力30MPa,时间0．5h,CO2流量20kg／h。     

乳铁蛋白抗炎及美白功效的体外试验研究

建立了丙二醇甲醚醋酸酯(PMA)诱导的人急性单核白血病细胞(THP-1)炎症模型,通过细胞毒性试验确定乳铁蛋白对THP-1的安全质量浓度,考察了乳铁蛋白对THP-1分泌白介素6(IL-6)的影响.研究结果表明:乳铁蛋白在最大安全质量浓度为0.1 g/L时能有效抑制IL-6的分泌,使IL-6的相对分泌量降至21.7％,抑...     

广藿香精油抗炎祛痘功效研究

采用气相色谱-质谱联用技术对广藿香精油成分进行分析。通过细胞毒性实验确定广藿香精油对人急性单核细胞(THP-1)的安全质量浓度。利用痤疮丙酸杆菌(P.acnes)刺激THP-1建立的特异性痤疮炎症细胞模型考察广藿香精油对炎症因子IL-1β的影响。通过荧光实时定量逆转录聚合酶链反应(qRT-PCR)初步探讨广藿香精油抗炎祛痘的作用机制。研究结果表明:广藿香精油在最大安全质量浓度12.5 mg/L时,对炎症细胞模型中P.acnes活性影响较小,对IL-1β的分泌具有明显的抑制作用(抑制率66.54%),可显著降低细胞内Toll样受体2(TLR2)mRNA的转录(抑制率73.73%),从而起到抑制炎症信号转导和继发性炎症介质生成的作用。     

大麻叶提取物的抗炎功效研究

通过观察以大麻叶提取物作用后斑马鱼尾鳍损伤部位巨噬细胞和中性粒细胞聚集和清除情况,研究大麻叶提取物的抗炎功效.结果表明:(1)在质量浓度1.0μg/mL和2.0μg/mL时,大麻叶提取物对斑马鱼巨噬细胞和中性粒细胞在斑马鱼尾鳍伤口处聚集有抑制作用,并相比对照具有显著性差异;(2)在质量浓度2.0μg/mL时,大麻叶提取...     

沙棘油的综合开发利用

沙棘油中含有维生素、三萜、脂类、黄酮类、酚类和微量元素等大量营养和生物活性物质,这些物质对于保护心血管系统、抗肿瘤、抗炎和搞衰老等方面有明显作用。作者根据沙棘油的这些特点,提出了在保健食品、药品和化妆品等方面综合开发和利用沙棘油的建议和意见。     

金银花野菊花牙膏的制备及抗炎作用评价

本文以金银花和野菊花提取物为基础,拟开发一款具有清热去火、消炎止痛抑菌作用的中草药牙膏。以摩擦剂碳酸钙、发泡剂十二烷基硫酸钠、稳定剂羧甲基纤维素钠、润湿剂丙三醇的添加量以及金银花野菊花提取物加入先后顺序为主要影响因素,通过单因素实验和正交实验优化金银花野菊花牙膏制备工艺,并对其抗炎效果进行评价。结果表明金银花野菊花牙膏的最佳配方组成为野菊花叶提取物0.5 g、金银花提取物0.5 g、丙三醇12.0 g、碳酸钙20.75 g、羧甲基纤维素钠0.6 g、十二烷基硫酸钠1.4 g、甲苯酸钠0.1 g、糖精钠0.2 g、香精0.4 g,按此配方制备得到的牙膏膏体细腻光亮、气味清香、黏稠度适中。同时金银花野菊花提取物对二甲苯致小鼠耳肿胀有明显的抑制作用,具有较为明显的抗炎效果。     

甘油磷酸肌醇胆碱盐抗敏感抗炎体外功效研究

以RBL-2H3肥大细胞模型测定了甘油磷酸肌醇胆碱盐对肥大细胞脱颗粒的影响;在PMA诱导的THP-1巨噬细胞模型上进一步测定了甘油磷酸肌醇胆碱盐对脂多糖诱导下的促炎因子、趋化因子释放的影响。结果表明,甘油磷酸肌醇胆碱盐在0.3%浓度时可显著抑制肥大细胞脱颗粒,从而表现出抗敏感活性;在0.01%～0.3%浓度内甘油磷酸肌醇胆碱盐可显著抑制炎症因子IL-6、TNF-α的释放,在0.1%～0.3%浓度内可显著抑制趋化因子IL-8、炎症因子IL-1β的释放,从而表现出优异的抗炎活性。     

Encapsulation of sea buckthorn kernel oil in modified starches

Encapsulation of CO₂-extracted sea buckthorn kernel oil and the stability of the products were investigated. Maltodextrin and an emulsifying starch derivative were used for encapsulation by spray drying. Both shell materials significantly increased the storage stability of sea buckthorn kernel oil, even though in maltodextrin capsules 10% of the total oil was extractable from the surface of the capsule. The cornstarch sodiium octenyl succinate derivative capsules contained essentially no surface oil. After 9 wk storage at controlled conditions (20°C, RH 50%), PV of the unencapsulated oil was above 90 meq/kg, whereas in the encapsulated oils, the PV was still around 20 meq/kg. The PV of the encapsulated oil was dependent on the storage conditions. A small increase in temperature (from 20 to 25–30°C) and a significant increase in humidity (from RH 50 to RH 50–70%) decreased the stability of capsules. This was associated with the physical state of the microcapsule matrix and may be linked with glass transition of the wall polymers.     

Tocopherols in sea buckthorn (<Emphasis Type="Italic">Hippophaë rhamnoides</Emphasis> L.) berry oil

The free tocopherol content in whole berries of six sea buckthorn cultivars grown in northeastern Poland and Belorussia was determined with HPLC. The total free tocopherol content in oil from whole berries was 101.4–128.3 mg/100 g of oil. α-Tocopherol was the predominant tocopherol of sea buckthorn berries, and only traces of γ-tocopherol were detected in the oil. α- and δ-Tocopherols constituted 62.5–67.9% and 32.1–37.5% of total tocopherol, respectively. The total free tocopherol content in oil of sea buckthorn cv. Nadbaltycka increased during maturation from 40.4 to 109.8 mg/100 g of oil. Green berries contained a marked amount of γ-tocopherol, but its content rapidly declined to traces when the color of berries turned from green to olive-yellow.     

Inclusion Complex of Sea Buckthorn Fruit Oil with β-Cyclodextrin: Preparation Characterization and Antioxidant Activity

This study aims to produce an inclusion complex between β-cyclodextrin (β-CD) and sea buckthorn fruit oil (SBFO) by using the saturated aqueous solution method and then to compare the resultant solution with the crude mixtures. In this study, the SBFO is extracted by Soxhlet extraction, which is optimized through the single-factor test. The main constituent of the SBFO is identified by gas chromatography-mass spectrometry (GC-MS), which confirms eight fatty acids. Fourier transform infrared spectroscopy, thermogravimetric analysis-differential scanning calorimetry, optical microscopy, scanning electron microscopy, and the result-characterized synthesized compounds confirm the formation of stable inclusion compounds by the SBFO with β-CD. The yield values of the inclusion complex and the SBFO loading content are 64.87 ± 2.26% and 9.2 ± 1.58%, respectively. Comparing the crude fruit oil, β-CD–SBFO, and saponified fruit oil reveals differences in their 2,2-diphenyl-1-pycrylhydrazyl and 2,2′-azinobis-(3-ethylbenzthiazoline-6-sulfonate) antioxidant activities. These findings suggest that the SBFO inclusion complex after β-cyclodextrin is stabilized and is a good antioxidant. This solution improves the inadequacy of the water solution and the instability of the SBFO. This study also implies that β-CD is a cost-effective and straightforward carrier for extending the applications of SBFO as a food or medicinal additive. Practical applications: In this experiment, the SBFO is converted from oil to powder to increase its efficacy as an additive to health and food products.     

Composition of phenolic acids in sea buckthorn (<Emphasis Type="Italic">Hippophae rhamnoides</Emphasis> L.) berries

The composition of phenolic acids in several varieties of sea buckthorn berries was determined by GC and MS. In six cultivars the total content of phenolic acids ranged from 3570±282 to 4439±405 mg per kg of berries, on a dry basis. Seventeen phenolic acids were tentatively identified in the berries. Salicylic acid was the principal phenolic acid in sea buckthorn berries, accounting for 55 to 74.3% of the total phenolic acids present. The phenolic acids liberated from esters and glycosidic bonds were the major fractions of phenolic acids in the berries, whereas free phenolic acids constituted only up to 2.3% of total phenolic acids present.     

Method for characterization of triacylglycerols and fat-soluble vitamins in edible oils and fats by supercritical fluid chromatography

This study demonstrates the usefulness of capillary supercritical fluid chromatography (SFC) for the characterization of triacylglycerols of edible oils and fats. Triacylglycerols were separated according to the acyl carbon number and the degree of unsaturation on a 25% cyanopropyl/25% phenyl/50% methylpolysiloxane stationary phase. Valuable information concerning the triacylglycerol composition of berry oils was obtained, despite the overlapping of certain triacylglycerol peaks. Simultaneous analysis of fat-soluble vitamins and triacylglycerols is not practical by capillary SFC with flame-ionization detection because of the low concentration of naturally-occurring fat-soluble vitamins in edible oils. Therefore, higher loading of the sample, which led to overloading of triacylglycerols, was required to get reasonable peaks for fat-soluble vitamins. The method was applied to the characterization of triacylglycerols and tocopherols in sea buckthorn pulp and seed oil, and cloudberry seed oil without any sample purification prior to SFC. In addition, the stationary phase proved useful for separating the more complex mixtures of triacylglycerols found in milk fat and in fish oil.     

全球牙膏市场与产品的最新进展

全球牙膏市场被划分为美国、日本、两欧、东欧、亚太、拉美和中东7个区域市场,介绍了各区域市场的牙膏销售最和产品特点。全球牙膏产品通常被划分为儿童、抗龋、抗过敏、牙龈保护、除牙斑、抗菌、美自、多效、新感觉和天然10个类别,同时,介绍了2003／2004年度各类别上市新品的最新进展。最后,文章对中外牙膏在市场、产品及管理方面的差异进行了分析,并提出相关的对策建议。     

对牙膏安全性评价的思考

从企业应重视产品安全性的角度出发,论述了对牙膏原料及牙膏产品安全性评价的重要性。同时阐明了功效型牙膏与特殊用途化妆品和保健食品在产品定位和作用特点上的相似之处,并且参考目前国家对特殊用途化妆品和保健食品安全性评价要求以及评价方法,提出了牙膏原料和牙膏产品的安全性评价建议。     

磨擦剂和牙膏pH值的控制

围绕牙膏磨擦剂在液相中的离解率和牙膏在口腔中的卫生安全性,通过建立牙膏酸碱度理论模型,对不同类型牙膏pH的合理控制范围进行了探讨,计算结果表明：CaCO3型、Al(OH)3型、CaHPO4型及SiO2型牙膏pH值合理控制分别为8．17～10．00、5．00～8．88、5．00～9．15和5．00～10．00。