Increasing the folate content in Egyptian baladi bread using germinated wheat flour

The main objective of this study was to increase the folate content in Egyptian baladi bread using germinated wheat flour (GWF). The effect of germination temperature and drying conditions on the folate content of wheat grains was studied. Wheat flour was substituted with unsieved and sieved GWF at different levels and the effects on folate content and the rheological properties of dough were determined. Germination of wheat grains resulted in a 3- to 4-fold higher folate content depending on the germination temperature. Maximum folate content (61 μg/100 g dry matter (DM)) occurred at 30 °C. Drying did not affect folate content in germinated grains. After replacement with GWF, folate content in both flour and bread increased 1.5- to 4-fold depending on the level of replacement. Rheological properties of dough were adversely affected by increasing replacement level (as determined by farinograph). While the folate content in bread was as high as 66 μg/100 g DM at complete replacement of flour with sieved GWF, the bread was dark and layers were not separated. After replacement of half of the flour with sieved GWF (50 g/100 g), the baladi bread was acceptable with respect to colour and layer separation. The folate content in this bread was 50 μg/100 g DM, compared with 30 μg/100 g DM in bread without GWF (0 g/100 g).     

In situ dextran synthesis by Weissella confusa Ck15 and Leuconostoc pseudomesenteroides DSM 20193 and their effect on chickpea sourdough bread

This work evaluated, for the first time, the impact of in situ dextran (with different branching degree) produced by Weissella confusa Ck15 and Leuconostoc pseudomesenteroides DSM 20193 strains on the technological properties of chickpea–wheat sourdough bread prepared with three levels of chickpea flour (20, 30 and 40 g/100 g). In addition Lactiplantibacillus plantarum F8 strain (not dextran producing) and a control without sourdough fermentation were used. Specific volume, crumb hardness and moisture content of breads were evaluated during six days of storage. At the increase of chickpea flour from 20 to 40 g/100 g in the samples, the lowest decrease in bread volume (15%) occurred when W. confusa Ck15 was used. Moreover, these breads showed the lowest crumb hardness at each chickpea flour percentage, 46, 80 and 98 N. Hence, in situ dextran synthesis by W. confusa Ck15 might counteract negative effects caused by gluten-free chickpea flour on technological properties of bread.     

Antihypertensive properties of aqueous extracts of vegetable leaf‐fortified bread after oral administration to spontaneously hypertensive rats

This study investigated the potential cardiovascular health benefits of leavened bread produced from wheat flour that contained 1%, 2% and 3% additions of leafy vegetable powders obtained from Amaranthus viridis (AO), Solanum macrocarpon (SM) or Telfairia occidentalis (TO). Dried breads were extracted with water at 60 °C followed by analysis for total polyphenolic content (TPC), as well as in vitro inhibitions of angiotensin‐converting enzyme and renin activities. HPLC analysis of the bread extracts indicated the presence of mainly rutin, gallic acid, myricetin and caffeic acid. TPC of the vegetable‐fortified breads was significantly (P < 0.05) higher (5.8–7.6 mg gallic acid equivalent, GAE/g) than that of control bread (5.5 mg GAE/g). Oral administration of 100 mg dried extract/kg body weight to spontaneously hypertensive rats led to reductions (up to 42 mmHg) in systolic, diastolic and mean arterial blood pressure in comparison with 20 mmHg for the control bread.     

Low folate content in gluten-free cereal products and their main ingredients

Folate content in some gluten-free cereal products and their main ingredients was determined using a validated method based on reversed-phase high performance liquid chromatography (HPLC) with fluorescence and diode array detection. The main folate forms found in gluten-free products were 5-methyl-tetrahydrofolate and tetrahydrofolate. Starches and low protein flours commonly used as main components in gluten-free products appeared to be poor folate sources with folate content ?6 μg/100 g fresh weight. Folate content in gluten-free breads was higher (15.1–35.9 μg folate/100 g fresh weight) due to use of bakery yeast which is a rich folate source. Overall, folate content in gluten-free products was lower than in their gluten-containing counterparts. Therefore, fortification of gluten-free products with folic acid or enrichment of these products with nutrient-dense fractions of cereals naturally free from gluten (such as buckwheat, quinoa, amaranth or millet) can be of interest.     

Effect of defatted maize germ flour addition on the physical and sensory quality of wheat bread

Maize (Zea mays L.) processing produces large quantities of defatted maize germ (DMG) that is being used mainly for animal feed. The objective of this study was to exploit use of this nutrient-rich by-product in bread by replacing wheat flour at 5-20 g/100 g levels. Breads prepared with wheat-DMG flour blends were analyzed for loaf volume, density, instrumental dough hardness and bread firmness, Hunter color (“L”, “a”, “b”, chroma, and hue angle), and selected sensory attributes. Loaf volumes decreased significantly, from 318.8 ml to 216.3 ml, as the DMG flour supplementation was increased from 0 to 20 g/100 g; a similar effect was observed for bread specific volume. Increase in dough hardness (7.56-71.32 N) was directly related to increase in DMG flour levels. Instrumental firmness values were significantly higher for breads containing DMG flours, 61.58 N in 20 g/100 g DMG bread versus 32.84 N for the control bread, made with wheat flour only. The control bread was lighter in color, as shown by higher “L” values, than those having DMG flour, with chroma and hue angle values significantly higher in treatment breads. In general, no differences were observed for the sensory attributes of crumb color, cells uniformity, aroma, firmness, mouthfeel, and off-flavor in breads with up to 15 g/100 g DMG flour, while the overall acceptability scores showed a mixed pattern. The results of this study demonstrated that acceptable quality bread could be made with DMG flour addition at ≤15 g/100 g.     

Effect of extruded wheat bran on dough rheology and bread quality

Bran extrusion is mainly under study and works should be performed to know the effect on dough and bread. This study was designed to investigate the effect of bran extrusion (addition of 2.5 g/100 g to 20 g/100g bran) on the rheological characteristics of bread dough, behaviour during fermentation, and bread quality. Extruded bran increased dough development time and tenacity to a greater extent than non-extruded bran, and minimized the loss of stability if over-mixing occurred. Extruded bran, due to its greater gas production, also reduced loss of dough height during fermentation to a greater extent than untreated bran. However, breads with extruded bran showed a higher volume and lower initial firmness than breads with normal bran if improver was added. However, no differences were found in the organoleptic evaluation. Bran extrusion therefore modified dough rheology but did not negatively affect bread quality. It could even improve the quality of breads with bran when improvers are added.     

Total phenolic content, consumer acceptance, and instrumental analysis of bread made with grape seed flour

Grape seed flour (GSF) from grape pomace, a waste product generated during winemaking, was explored for use in bread production due to its potential health benefits. This study evaluated the consumer acceptance and physical properties of bread, including total phenolic content (TPC), made with varying levels of GSF. Dough and breads were prepared using different levels of replacement of hard red spring wheat flour (HRS) with GSF (0 to 10 g GSF/100 g HRS) and stored for 0, 2, or 6 wk at -20 °C. Replacement of 10 g GSF/100 g HRS increased the bread TPC from 0.064 mg tannic acid/g dry weight to 4.25 mg tannic acid/g dry weight. Consumer acceptance and instrumental analyses were used to investigate changes in sensory and texture properties due to GSF replacement. Replacement above 5 g GSF/100 g HRS decreased the loaf brightness and volume, with an increase in the bread hardness and porosity. Generally, breads containing ≥ 7.5 g GSF/100 g HRS were characterized by lower consumer acceptance. A reduction in overall and bitterness acceptance was observed in bread at 10 g GSF/100 g HRS, with decreased acceptance of astringency and sweetness at 7.5 and 10 g GSF/100 g HRS. Based on these results, the replacement of 5 g GSF/100 g HRS is recommended for the production of fortified breads with acceptable physical and sensory properties and high TPC activity compared to refined bread. PRACTICAL APPLICATIONS: This study shows that grape seed flour (GSF) can be used to replace hard red spring wheat flour (HRS) in bread production, with moderate impact on the physical and sensory properties of the bread. Replacement of up to 10 g GSF/100 g HRS significantly decreased overall consumer acceptance of the bread, with lower consumer acceptance of sweetness and astringency at 7.5 and 10 g GSF/100 g HRS. Thus, a replacement value of 5 g GSF/100 g HRS is recommended for the production of fortified breads.     

Nutritional,microbial and sensory attributes of bread fortified with defatted watermelon seed flour

The utilisation of natural food supplements to fortify staples is considered as one of the effective means of dealing with global food nutrient deficiency. Herein, we report the nutritional, microbial and sensorial characteristics of defatted watermelon seed fortified bread. The protein content and level of micronutrients such as phosphorus, potassium, iron and calcium increased with increasing proportion of watermelon seed flour in the bread. Notably, calcium resulted as the highest micronutrient in the bread samples, whereas the content of iron in the fortified samples improved significantly. The defatted watermelon seed flour had a high iron content of about 12.1 mg/100 g as compared to the normal (unfortified) bread of 0.01 mg/100 g. The total microbial count results for all the fortification levels complied with the acceptable microbial quality requirement. Lastly, the consumer acceptability test did not show any significant difference between all levels of fortification with watermelon seed flour.     

Biofortification of folates in white wheat bread by selection of yeast strain and process

We here demonstrate that folate content in yeast fermented food can be dramatically increased by using a proper (i) yeast strain and (ii) cultivation procedure for the selected strain prior to food fermentation. Folate levels were 3 to 5-fold higher in white wheat bread leavened with a Saccharomyces cerevisiae strain CBS7764, cultured in defined medium and harvested in the respiro-fermentative phase of growth prior to dough preparation (135-139 microg/100 dry matter), compared to white wheat bread leavened with commercial Baker's yeast (27-43 microg/100 g). The commercial Baker's yeast strain had been industrially produced, using a fed-batch process, thereafter compressed and stored in the refrigerator until bakings were initiated. This strategy is an attractive alternative to fortification of bread with synthetically produced folic acid. By using a high folate producing strain cultured a suitable way folate levels obtained were in accordance with folic acid content in fortified cereal products.     

Quality and antioxidant property of buckwheat enhanced wheat bread

Common buckwheat (Fagopyrium esculentum Moench) was used to substitute 15% of wheat flour to make buckwheat enhanced wheat breads. Proximate composition, physical quality, functional components and antioxidant properties of buckwheat enhanced wheat breads were analysed and compared with those of white bread. Specific volumes of three breads were 6.10–6.75 cm³/g. Buckwheat enhanced wheat bread showed lower lightness and whiteness index values and higher redness and yellowness values. On a seven-point hedonic scale, all sensory results were 5.33–5.91, indicating that three breads were moderately acceptable. No differences were found in appearance, colour and overall sensory attributes for three breads, whereas both buckwheat enhanced wheat breads were rated higher in flavour and mouth feel. Buckwheat enhanced wheat bread contained more rutin and quercetin as expected. Buckwheat enhanced wheat bread was good in antioxidant activity, reducing power and 1,1-diphenyl-2-picrylhydrazyl radical scavenging ability with unhusked buckwheat enhanced wheat bread being the most effective. Overall, buckwheat enhanced wheat bread could be developed as a food with more effective antioxidant properties.     

The consumer acceptance of novel vegetable-enriched bread products as a potential vehicle to increase vegetable consumption

Currently UK fruit and vegetable intakes are below recommendations. Bread is a staple food consumed by approximately 95% of adults in western countries. In addition, bread provides an ideal matrix by which functionality can be delivered to the consumer in an accepted and convenient food. Therefore, enriching bread with vegetables may be an effective strategy to increase vegetable consumption. This study evaluated bread enriched with red beetroot, carrot with coriander, red pepper with tomato or white beetroot (40 g vegetable per 100 g) compared to white control bread (0 g vegetable) for consumer acceptance. Consumers (n = 120) rated their liking of the breads overall, as well as their liking of appearance, flavour and texture using nine-point hedonic scales. Product replacement and purchase intent of the breads were rated using five-point scales. The effect of providing consumers with health information about the breads was also evaluated. There were significant differences in overall liking (P < 0.0001), as well as liking of appearance (P < 0.0001), flavour (P = 0.0002) and texture (P = 0.04), between the breads. However, the significant differences resulted from the red beetroot bread which was significantly (P < 0.05) less liked compared to control bread. There were no significant differences in overall liking between any of the other vegetable-enriched breads compared with the control bread (no vegetable inclusion). The provision of health information about the breads did not increase consumer liking of the vegetable-enriched breads. In conclusion, this study demonstrated that vegetable-enriched bread appeared to be an acceptable strategy to increase vegetable intake, however, liking depended on vegetable type.     

The impacts of germinating organic wheat: effects on phytic acid,resistant starch,and functional properties of flour,and sensory attributes of sourdough bread

This study assessed the impact of germination duration on two varieties of wheat grown organically (Expresso and Syngenta SY Basalt (Basalt)). In Expresso, d -glucose content increased from 0.06 g/100 g in the control to 0.55 g/100 g after a 24-h germination period (P < 0.05). In both varieties, similar increases were observed for sucrose and maltose, and no pattern emerged for resistant starch or phytate. In Basalt, baseline phytate (0 h 1.07 g/100 g) did not differ from treatment groups (6 h 0.97 g/100 g; 12 h 0.98 g/100 g; 24 h 1.09 g/100 g; 36 h 0.98 g/100 g). Functional properties of flour decreased with germination duration and resulted in bread which panellists perceived to have inferior crumb (24 h Expresso; 36 h Basalt; P < 0.05). However, there was no difference in the overall acceptability of breads across treatments and inclusion of germinated wheat improved bread sweetness.     

Effect of flaxseed flour addition on physicochemical and sensory properties of functional bread

The importance of polyunsaturated fatty acids (PUFA) in health and nutrition is well recognised. Flaxseed (Linum usitatissimum) has recently gained a lot of attention as functional food because of its unique nutrient profile. In the present work efforts were made to develop omega-3 enriched functional bread using raw and roasted ground flaxseed flour. Initially optimisation of each bread ingredient viz., salt, sugar, and shortening, GMS, yeast and water was carried out on the basis of sensory overall acceptability score. The standardised bread was incorporated with raw and roasted ground flaxseed (5, 10, and 15 g/100 g) flour. The effect of flaxseed incorporation on bread dough rheology parameters viz., dough stickiness and water absorption was studied. Increase in water absorption and dough stickiness was observed with increased flaxseed level. Further breads were evaluated for sensory parameters, colour and texture. The crumb softness increased with increase in flaxseed level. Bread was optimised at 10(g/100 g) flaxseed level based on sensory evaluation.     

Dry-fractionation of wheat bran increases the bioaccessibility of phenolic acids in breads made from processed bran fractions

This study evaluated the potential of using ultra-fine grinding and electrostatic separation of wheat bran as methods to improve the bioaccessibility of para-coumaric acid (pCA), sinapic acid (SA) and ferulic acid (FA) from bran-rich breads. Bran fractions were produced and used to bake white bread, whole-grain bread, and seven different bran-rich breads. The influence of bran particle size and bread composition on the bioaccessibility of pCA, SA and FA was studied using a dynamic computer-controlled in vitro gastro-intestinal model. The amount of bioaccessible phenolic acids was higher in whole-grain bread and bran-rich breads than in white bread, and the finer the bran particles in bran-rich breads, the more bioaccessible the phenolic acids. The highest amounts of bioaccessible phenolic acids were observed for two of the fractions obtained by electrostatic separation of ground bran. Only the free and conjugated phenolic acids forms were found to be bioaccessible, and the bioaccessibility of SA was much higher than that of FA, due to the higher solubility of SA. This study demonstrated that the use of bran fractionation to reduce the particle size, or to include only some parts of the bran in foods, can help developing grain-based products with increased nutritional potential.     

Gluten-Free Bread Making Trials from Cassava (Manihot Esculenta Crantz) Flour and Sensory Evaluation of the Final Product

The aim of this work was to evaluate the effectiveness of the use of cassava flour in bread making, and the sensory acceptability of the final product. Different baking trials were carried out by using egg white and extra-virgin olive oil, in consideration of their high nutritional value with respect to other food additives (i.e., hydrocolloids). Significant (p < 0.05) improvements of loaf specific volume (from 2.24 to 3.93 mL/g) and crumb firmness (from 9.14 to 4.67 N) were achieved by contemporarily including egg white and extra-virgin olive oil in the formulation. Cassava breads containing both these ingredients obtained the best scores from panelists for all the test breads examined and resulted attractive as the wheat bread prepared as reference.     

Determination of folate in some cereals and commercial cereal-grain products consumed in Poland using trienzyme extraction and high-performance liquid chromatography methods

A trienzyme treatment (rat plasma conjugase, -amylase, protease) followed by affinity chromatography (with folate-binding protein from bovine milk) and reversed-phase high-performance liquid chromatography with UV and fluorescence detections was performed for quantification of different folate derivatives in some commercial cereal-grain products and cereals cultivated in Poland. The method described allowed good separation of the main folate vitamins. Of the commercial cereal-grain products analyzed, buckwheat groats (30 g/100 g) had the highest content of total folate, followed by rye flour (29 g/100 g), barley groats (21 g/100 g) and wheat flours (19–20 g/100 g). Two of the ready-to eat breakfast products were found to be fortified with folic acid with the level slightly exceeding the label declaration. The total folate content of whole-grain wheat flours was more than half as much as in white flours (33–40 g/100 g), and the highest folate content was found in rye whole grains (123–135 g/100 g). 5-Formyltetrahydrofolate was the predominating folate form in the cereal products analyzed, above all in rye flour.     

Effects of hull-less barley flour and flakes on bread nutritional composition and sensory properties

Barley is a desirable food ingredient, with health benefits provided by a β-glucan fibre fraction. A hull-less barley flour and flakes were incorporated into white and wholegrain wheat bread in quantities usually applied in practice. The breads were evaluated for nutritional composition and sensory properties and compared to standard products as controls. The supplemented breads were high in fibre, zinc and selenium content. It was estimated that a 300-gram daily portion of such breads could meet up to 40% of dietary recommended intakes for selenium and 70–75% of recommended daily values for β-glucan. Regarding sensory quality, the only significant differences (p < 0.05) were higher taste and lower volume in the white supplemented breads and lower crumb elasticity in the white bread made with barley flour. Hull-less barley can substantially contribute to an adequate intake of selenium and β-glucan. In addition, supplemented breads were not found to pose a significant risk, with regard to excessive intakes of heavy elements (Pb, Cd, As).     

Enrichment of Egyptian ‘Balady’ bread. Part 2. Nutritional values and biological evaluation of enrichment with decorticated cracked broadbeans flour (Vicia fabaL./ 1

Decorticated cracked broadbeans flour (DCBF) was used to replace 5%, 10%, 15% and 20% of the wheat flour (WF) in the Egyptian ‘Balady’ bread. The nutrient composition of DCBF, WF, wheat bread, and DCBF‐fortified bread was studied. When DCBF fortification was increased from 0 to 20%, there was an increase of 36% in protein, 18% in fat, 123% in calcium, 52% in phosphorus and 40% in iron contents. DCBF contained greater amounts [g/16 g N] of lysine and histidine compared to WF. All essential amino acids increased when DCBF was substituted for WF from 0 to 20%, except methionine, which decreased. The biological quality of the breads was investigated. The protein efficiency ratio for 10%. DCBF bread (1.60) was found to be significantly greater (P > 0.05) than that of 5%‐DCBF bread (1.48) and wheat bread (1.17).     

Glycemic Index and Chemical Composition of Traditional Omani Breads

This study evaluated the chemical composition and glycemic index (GI) of eight different types of traditional Omani breads made from wheat flour. Significant (P < 0.05) differences were observed in the proximate chemical composition, dietary fibre content, and gross energy value of these breads. The moisture, crude protein, total fat, ash, crude fibre and nitrogen free extract (NFE) contents ranged between 21.6–51.6, 4.3–10.5, 0.8–13.8, 1.2–2.3, 1.0–2.0, and 34.5–61.8 g/100 g of sample respectively. The values for dietary fibre were between 5.6, 9.6 g/100g, and the gross energy contents ranged between 897 and 1468 kJ/100 g. The GI value of these breads also differed significantly (P < 0.05). No significant differences were however observed among the GI values for White Toast Bread (local commercial bread), Brown Toast Bread (local commercial bread), White Khubz Lebanani (local commercial bread), Brown Khubz Lebanani (local commercial bread), and Chapati (local homemade bread), which were 63 ± 12, 58 ± 15, 63 ± 10, 57 ± 20, and 58 ± 10, respectively. Paratha (local restaurant made bread with fat), Goleh (local homemade soft bread), and Rekhal (local homemade thin bread) showed significantly (P < 0.05) lower GI values as compared to other breads, and these were 32 ± 13, 43 ± 13 and 39 ± 7, respectively. No specific correlation was observed in the chemical composition and glycemic index of these breads. These breads fall within low to medium GI category of foods.     

X-ray micro-computed tomography evaluation of bubble structure of freeze-dried dough and foam properties of bread produced from roasted wheat flour

This study evaluated the bubble structure of freeze-dried dough and foam properties of bread produced from the roasted wheat flour by means of X-ray micro-computed tomography (μCT). Wheat was roasted at 90 °C and 86 Hz, of which the conditions was determined by means of a central composite design, and the flour used to produce dough proofed for 20- and 40 min as well as bread. The use of 10 g dough and bread samples enabled scanning at a much higher resolution. Roasting resulted in a decreased strut thickness of the bread, suggesting a finer crumb structure and softer texture, compared to the control. Porosity increases were observed for the roasted wheat samples. This suggested a slightly weaker gluten strength as was observed with the lower mixograph peak height. Roasting wheat, at the conditions determined by the central composite design, did not negatively affect the foam properties of the breads produced from the roasted wheat flour.