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Click‐Chemistry Based Allergen Arrays Generated by Polymer Pen Lithography for Mast Cell Activation Studies
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Ravi Kumar Alice Bonicelli Sylwia Sekula‐Neuner Andrew C. B. Cato Michael Hirtz Harald Fuchs 《Small (Weinheim an der Bergstrasse, Germany)》2016,12(38):5330-5338
The profiling of allergic responses is a powerful tool in biomedical research and in judging therapeutic outcome in patients suffering from allergy. Novel insights into the signaling cascades and easier readouts can be achieved by shifting activation studies of bulk immune cells to the single cell level on patterned surfaces. The functionality of dinitrophenol (DNP) as a hapten in the induction of allergic reactions has allowed the activation process of single mast cells seeded on patterned surfaces to be studied following treatment with allergen specific Immunoglobulin E antibodies. Here, a click‐chemistry approach is applied in combination with polymer pen lithography (PPL) to pattern DNP‐azide on alkyne‐terminated surfaces to generate arrays of allergen. The large area functionalization offered by PPL allows an easy incorporation of such arrays into microfluidic chips. In such a setup, easy handling of cell suspension, incubation process, and read‐out by fluorescence microscopy will allow immune cell activation screening to be easily adapted for diagnostics and biomedical research. 相似文献
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《Journal of Modern Optics》2013,60(7):815-826
Any modification of the uniform amplitude distribution in the pupil of an optical system by a non-uniformly absorbing filter leads to changes in its spread function. Radially symmetrical pupil masks with a continuously varying transmittance have been used for this purpose. These can be made by vacuum deposition or photographic integration, but the manufacturing process is difficult and involves the use of rotating masks. An alternative is to use a binary mask consisting of a concentric array of annuli which approximates to the continuously varying absorption. The potential of such annular pupils for modifying the point spread of a confocally scanned imaging system is discussed, and some experimental results are presented. 相似文献
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Heyou Zhang Calum Kinnear Paul Mulvaney 《Advanced materials (Deerfield Beach, Fla.)》2020,32(18):1904551
To realize the full potential of nanocrystals in nanotechnology, it is necessary to integrate single nanocrystals into addressable structures; for example, arrays and periodic lattices. The current methods for achieving this are reviewed. It is shown that a combination of top-down lithography techniques with directed assembly offers a platform for attaining this goal. The most promising of these directed assembly methods are reviewed: capillary force assembly, electrostatic assembly, optical printing, DNA-based assembly, and electrophoretic deposition. The last of these appears to offer a generic approach to fabrication of single-nanocrystal arrays. 相似文献
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In this paper, we present a simple method to fabricate ultra-high-density hexagonal arrays of ferromagnetic nanorings having 13?nm outer diameter, 5?nm inner diameter and 5 nm thickness. Cobalt magnetic nanorings were fabricated using a self-assembled diblock copolymer template with an angular evaporation of metal followed by an ion-beam etching. Magnetic measurements and theoretical calculations suggest that, at low fields, only the single domain and vortex states are important for rings of this size. The measured magnetization as a function of applied field shows a hysteresis that is consistent. These ultrasmall ferromagnetic rings have potential use in magnetic memory devices due to the simplicity of the preparation coupled with the ultra-high-density and geometry-controlled switching. This fabrication technique can be extended to other materials for applications in optics, sensing and nanoscale research. 相似文献
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S. J. Smith S. R. Bandler A.-D. Brown J. A. Chervenak E. Figueroa-Feliciano F. Finkbeiner N. Iyomoto R. L. Kelley C. A. Kilbourne F. S. Porter 《Journal of Low Temperature Physics》2008,151(3-4):1009-1014
We are developing position-sensitive transition-edge sensors (PoSTs) for future X-ray astronomy missions such as NASAs Constellation-X.
The PoST consists of 1 or more transitions-edge sensors (TESs) thermally connected to a large X-ray absorber, which through
heat diffusion, gives rise to position dependence. The development of PoSTs is motivated by the desire to achieve the largest
focal-plane coverage with the fewest number of readout channels. In this paper, we investigate optimized signal processing
algorithms for single channel PoSTs or ‘Hydras’ (consisting of 4-absorbers connected to 1 TES). Using simulated data, we investigate
the impact different parameters such as the thermal conductances and the inductance of the bias circuit have on device performance.
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Abstract Imaging in a scanning optical microscope with a detector consisting of an array of rings is considered. It is found that both transverse and axial resolution can be increased simultaneously. 相似文献
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Two dimensional Josephson junction arrays (JJAs) offer theopportunity to study a variety of basic physical concepts. Thepresent review focuses on recent experimental work on thedynamics of JJAs, as characterized by ac conductancemeasurements. The review starts with a discussion of basicphysics necessary to describe JJAs. Some experimentalissues, array fabrication and measurement techniques areconsidered next. In a perpendicular magnetic field, a JJA isan experimental realization of the frustrated XY model, withthe frustration parameter f, corresponding to the number offlux quanta in a unit cell of the array, adjusted by themagnetic field. It is thereby possible to investigate thenature of the ground states at arbitrary frustrations. Phasetransitions are the next topic: the vortex unbindingtransition is observed at integer f-values, while, if thejunction coupling energies are appropriately varied across thearray, at half-integer f-values the Ising transition,associated with chiral symmetry breaking, may be observed.Some aspects of vortex dynamics, a subject which is not yetcompletely understood, are then considered. Under certainconditions there is virtually no pinning in JJAs, they aretherefore ideally suited for the study of vortex dynamics. Thenext topic of this review is concerned with the influence ofdisorder on the ground states and on the phase transitions inJJAs. Site percolation in JJAs has provided some insightsinto the physics of disordered systems and allowed to verifysome theoretical predictions on percolation in two dimensions.A quick look at JJAs in the underdamped regime concludesthis review. 相似文献
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For the first time single crystalline gold nanobelt arrays with identical crystallographic orientation were obtained. A combined method consisting of directional solid-state transformation of a Fe-Au eutectoid and a well controlled electrochemical treatment enables production of arrays of nanobelts with a desired length. They have an average thickness of 25 nm and width of 200-250 nm, respectively. The obtained gold nanobelt arrays were characterized by electron backscattered diffraction (EBSD), X-ray diffraction, and XPS. The underlying mechanisms and the potential of this method for the production of nanosensors are discussed. 相似文献
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Yafang Tu Qiuming Fu Xiaojuan Niu Jianping Sang Zhijie Tan Xianwu Zou 《材料科学技术学报》2013,29(11):1053-1058
SnO2 nanotube arrays have been synthesized by means of a simple and low-cost method. The ZnO nanorod arrays prepared by aqueous chemical growth method were used as templates. By liquid phase deposition, SnO2 nanotubes were obtained with proper deposition time. Scanning electron microscopy, transmission electron microscopy and X-ray diffraction were used to characterize the morphologies and structures of the products, and the formation mechanism was discussed according to the experimental results. 相似文献